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http://dx.doi.org/10.3795/KSME-B.2012.36.11.1119

Analysis of Three-Dimensional Profile of Bacterial Colony and Visualization of Fluidic Biofilm Using Fluorescent Microbeads  

Kim, Kyung-Hoon (Dept. of Mechanical Engineering, Graduate School, Kookmin Univ.)
Park, Eun-Jung (Dept. of Mechanical Engineering, Graduate School, Kookmin Univ.)
Kim, Jung-Kyung (School of Mechanical Systems Engineering, Kookmin Univ.)
Publication Information
Transactions of the Korean Society of Mechanical Engineers B / v.36, no.11, 2012 , pp. 1119-1126 More about this Journal
Abstract
The collective behavior of bacteria plays an important role in biofilm development. In this study, the fluidic properties of biofilms formed in Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) colonies were compared by visualizing 200-nm fluorescent beads that were initially embedded in an agar plate and distributed spontaneously on the upper surface of the growing colonies. We conducted experiments to measure the three-dimensional profile of the E. coli colony using fluorescent microbeads that did not flow in the colony. Vortical flow patterns near the edge of the B. subtilis colony were observed clearly by tracking the movement of the beads in the biofilm of the colony. The present study should be the first step toward determining the effect of fluidic biofilms on the growth and swarming dynamics of bacteria.
Keywords
Bacterial Colony Growth; Bacterial Swarming; Biofilm; Fluorescent Particles; Diffusion;
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