• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.10
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• pp.6567-6574
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• 2015

In order to estimate the characteristics of the growth and composition of phytoplankton according to the available nutrients, we added nitrate (0, 1, 5, 10, 20, 50, $100{\mu}M$) and phosphate (0, 0.1, 0.5, 1, 2, 5, $10{\mu}M$) to field samples in a eutrophic site (St. 1) and an oligotrophic site (St. 22) in 2010 as well as a eutrophic site (St. 1, 5), a mesotrophic site (St. 19), and an oligotrophic site (St. 22) in 2011 at Jinhae Bay, Korea. The phytoplankton growth in the areas with additional nitrates and phosphates on St. 1 were significantly different from the control (One-way ANOVA:P<0.01). The dominant species at St. 1 in 2010 were Heterocapsa triquetra and Pseudo-nitzchia spp., to which nitrate and phosphate were added, respectively. The dominant species at St. 22 in 2010 differed between treatment conditions as follows: nitrate treatment Chaetoceros spp. (${\leq}10{\mu}M$), Thalassiosira spp. ($20{\mu}M$), and Pseudo-nitzchia spp.(${\geq}50{\mu}M$) for nitrate treatment; Cylindrotheca spp. ($2{\mu}M$) and Pseudo-nitzchia spp. ($5{\mu}M$) for phosphate treatment. Phytoplankton growth in 2011 according to the added nutrient were significantly different with treatment concentrations (One-way ANOVA: P<0.01). Moreover, the beginning of exponential growth in phytoplanktons was different between the eutro-mesotrophic sites (St. 1, 5, and 19) and the oligotrophic sites (St. 22) on day 2 and day 6 respectively. This implies that phytoplankton growth in the low nutrient condition may be retarded. The dominant species at St. 1 were Eucampia spp. and Chaetoceros spp. in the low nutrient treatment compared to Skeletonema spp., and Thalassiosira spp in the high nutrient treatment. The dominant species at St. 5 and St. 19 were mostly Skeletonema spp. and Chaetoceros spp. However, the dominant species at St. 22 was Thalassiosira spp.. The results of this study showed that phytoplankton growth and composition were different in areas with different nutrient characteristics resulting from the additional nutrients. Therefore, the nutrients additional algal assay could be indirectly explained why the biomass and composition of phytoplankton in Jinhae Bay has shown spatial differences.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.116-123
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• 2018

The sperm quality is determined by the kinetic characteristics and acrosome integrity of the sperm. In the previous studies, analysis of semen quality had large errors because those experiments by using microscope had been conducted by people. In recent years, the molecular biological methods have been newly developed to complement the previous techniques. The ZAR1 gene is known to be a gene that affects early embryonic development in vertebrates, but there is no study of the association with semen. In this study, we analyzed the association between the kinetic characteristics and ZAR1 single nucleotide polymorphism (SNP) genotype. To detect the SNPs, we performed sequencing using genomic DNA from the whole bloods of Duroc pigs. We identified an SNP in the ZAR1 gene g.2540T>C. ZAR1 SNP genotypeing in 105 pigs revealed that the major and minor alleles were T and C, respectively. After we analyzed the association between the kinetic characteristics of sperm and the ZAR1 SNP genotype, we found a significant association in MOT (p<0.01), VSL (p<0.05) of the kinetic characteristics in the Duroc boars. It was confirmed that the boars with T allele were lower in MOT and VSL than C allele. Therefore, pigs with C allele are judged to be better at the MOT and VSL of semen. Based on these results, ZAR1 SNP genotyping may be a useful molecular biomarker to improve semen quality by applying molecular breeding technology.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.265-275
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• 2002

Flavonoid biosynthesis is one of the most extensively studied areas in the secondary metabolism. Due to the study of flavonoid metabolism in diverse plant system, the pathways become the best characterized secondary metabolites and can be excellent targets for metabolic engineering. These flavonoid-derived secondary metabolites have been considerably divergent functional roles: floral pigment, anticancer, antiviral, antitoxin, and hepatoprotective. Three species have been significant for elucidating the flavonoid metabolism and isolating the genes controlling the flavonoid genes: maize (Zea mays), snapdragon (Antirrhinum majus) and petunia (Prtunia hybrida). Recently, many genes involved in biosynthesis of flavonoid have been isolated and characterized using mutation and recombinant DNA technologies including transposon tagging and T-DNA tagging which are novel approaches for the discovery of uncharacterized genes. Metabolic engineering of flavonoid biosynthesis was approached by sense or antisense manipulation of the genes related with flavonoid pathway, or by modified expression of regulatory genes. So, the use of a variety of experimental tools and metabolic engineering facilitated the characterization of the flavonoid metabolism. Here we review recent progresses in flavonoid metabolism: confirmation of genes, metabolic engineering, and applications in the industrial use.

• Journal of Mushroom
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• v.18 no.1
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• pp.1-9
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• 2020

Mycorrhiza refers to the association between a plant and a fungus colonizing the cortical tissue of the plant's roots during periods of active plant growth. The benefits afforded by plants from mycorrhizal symbioses can be characterized either agronomically, based on increased growth and yield, or ecologically, based on improved fitness (i.e., reproductive ability). In either case, the benefit accrues primarily because mycorrhizal fungi form a critical linkage between plant roots and the soil. The soilborne or extramatrical hyphae take up nutrients from the soil solution and transport them to the root. This mycorrhizae-mediated mechanism increases the effective absorptive surface area of the plant. There are seven major types of mycorrhizae along with mycoheterotrophy: endomycorrhizae (arbuscular mycorrhizae, AM), ectomycorrhizae (EM), ectendomycorrhizae, monotropoid, arbutoid, orchid, and ericoid. Endomycorrhizal fungi form arbuscules or highly branched structures within root cortical cells, giving rise to arbuscular mycorrhiza, which may produce extensive extramatrical hyphae and significantly increase phosphorus inflow rates in the plants they colonize. Ectomycorrhizal fungi may produce large quantities of hyphae on the root and in the soil; these hyphae play a role in absorption and translocation of inorganic nutrients and water, and also release nutrients from litter layers by producing enzymes involved in mineralization of organic matters. Over 4,000 fungal species, primarily belonging to Basidiomycotina and to a lesser extent Ascomycotina, are able to form ectomycorrhizae. Many of these fungi produce various mushrooms on the forest floor that are traded at a high price. In this paper, we discuss the benefits, nutrient cycles, and artificial cultivation of mycorrhizae in Korea.

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.350-363
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• 2009

Inter- and intra-specific differences in removal activities, filtering rates (FR) and production of feces-and pseudo-feces (PF) between a native freshwater bivalve in Korea, Anodonta woodiana Lea and Unio douglasiae Griffith et Pidgeon, were compared using a continuous removal of organic matters (CROM) system. The CROM system comprised five steps; input of polluted water, control of water flow, mussel treatment, analysis of water quality and discharge of clean water. The study was designed to compare the removal activity of organic matters between A. woodiana and U. douglasiae, and the intra-specific differences between density and length in A. woordiana. Results clearly indicate that two kinds of mussels had obvious removal activities of seston in the eutrophic reservoir. First, if both are similar in shell length, there were no significant inter-specific differences in removal activity between A. woordiana and U. douglasiae (P>0.5), but FRs of U. douglasiae was relatively high due to low ash-fee dry weight. Second, if both are same in animal density, the smaller mussels (1$\sim$2 years old) showed a higher filtering rate and production of feces- and pseudo-feces and less release of ammonium than the larger mussels. Third, if both are same in biomass, FRs and PF of mussels were higher in the low-density tank than the high-density tank, While the Concentration of $NH_4$-N and $PO_4$-P released WRS similar to each other (P>0.5). Therefore, these results suggest that CROM system using a young bivalve A. woordiana can be applied to control the nuisance seston in eutrophic lake system, if a relevant species and density were selected. Additional pilot tests to optimize the age and density of domestic bivalves were needed for the generalization of CROM operation.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.158-167
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• 2016

The requirements for medical laboratories ISO 15189 is examined in organization and a quality management system, stressing the importance of evidence, document control, and control of records and clinical material. Medical services are provided from the areas of resource management, and pre-examination, examination and post-examination processes. The main goal of ISO 15189 accreditation is to improve the quality of laboratory services provided for patients and clinical users not only through compliance with consensually developed and harmonized requirements but also by adopting the philosophy of continual improvement using the Plan-Do-Check-Act cycle. Laboratory quality should be evaluated and improved in all steps of the testing process as the state-of-the art indicates that the pre- and post-analytical phases are more vulnerable to errors than the intra-analytical phase. The Korea Laboratory Accreditation Scheme (KOLAS), a national accreditation body, provides medical laboratory accreditations for appropriate approaches to evaluating the competence of a medical laboratory in providing effective services to its customers and clinical users. Adoption of ISO 15189 in 2010s as a government policy has been delayed, and only 5 laboratories have been accredited to date in Korea. The medical laboratories should seek the adoption of ISO 15189 with a positive attitude for quality improvement and strengthening of international competitiveness.

• Journal of Soil and Groundwater Environment
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• v.10 no.2
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• pp.35-43
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• 2005

Bioslurping combines the three remedial approaches of bioventing, vacuum-enhanced free-product recovery, and soil vapor extraction. Bioslurping is less effective in tight (low-permeability) soils. The greatest limitation to air permeability is excessive soil moisture. Optimum soil moisture is very soil-specific. Too much moisture can reduce air permeability of the soil and decrease its oxygen transfer capability. Too little moisture will inhibit microbial activity. So Modified Fenton reaction as chemical treatment which can overcome the weakness of Bioslurping was experimented for simultaneous treatment. Although the diesel removal efficiency of SVE process increased in proportion to applied vacuum pressure, SVE process was difficulty to remediation quickly semi- or non-volatile compounds absorbed soil strongly. And SVE process had variation of efficiency with distance from the extraction well and depth a air flow form of hemisphere centering around the well. Below 0.1 % hydrogen peroxide shows the potential of using hydrogen peroxide as oxygen source but the co-oxidation of chemical and biological treatment was impossible because of the low efficiency of Modified Fenton reaction at 0.1 % (wt) hydrogen peroxide. NTA was more efficiency than EDTA as chelating agent and diesel removal efficiency of Modified Fenton reaction increased in proportion to hydrogen peroxide concentration. Hexadecane as typical aliphatic compound was removed less than Toluene as aromatic compound because of its structural stability in Modified Fenton reaction. What minimum 10% hydrogen peroxide concentration has good remediation efficiency of diesel contaminated groundwater may show the potential use of Modified Fenton reaction after bioslurping treatment.

• The Korean Journal of Nuclear Medicine Technology
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• v.18 no.1
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• pp.3-9
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• 2014

Purpose: Our goals were to evaluate the effect of high dose radioiodine treatment for thyroid cancer by taking in laxatives. Materials and Methods: Twenty patients(M:F=13:7, age $46.3{\pm}8.1\;yrs$) who underwent high dose radioiodine treatment were seperated into Group 1 taking $^{131}I$ 5,500 MBq and Group 2 with the use of laxatives after taking $^{131}I$ 5,500 MBq. The whole body was scanned 16 hours and 40 hours after taking radioactive iodines by using gamma camera, the ROIs were drawn on the gastro-intestinal tract and thigh for calculation of reduction ratio. At particular time during hospitalization, the radioactivity remaining in the body was measured in 1 meter from patient by using survey meter (RadEye-G10, Thermo Fisher Scientific, USA). Schematic presentation of an Origin 8.5.1 software was used for spatial dose rate. Statistical comparison between groups were done using independent samples t-test. P value less than 0.05 was regarded as statistically significant. Results: The reduction ratio in gastro-intestinal 16 hours and 40 hours after taking laxatives is $42.1{\pm}6.3%$ in Group 1 and $72.1{\pm}6.4%$ in Group 2. The spatial dose rate measured when discharging from hospital was $23.8{\pm}6.7{\mu}Sv/h$ in Group 1 and $8.2{\pm}2.4{\mu}Sv/h$ in Group 2. The radioactivity remaining in the body is much decreased at the patient with laxatives(P<0.05). Conclusion: The use in combination with laxatives is helpful for decreasing radioactivity remaining in the body. The radioactive contamination could be decreased at marginal individuals from patients.

• The Korean Journal of Nuclear Medicine Technology
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• v.12 no.3
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• pp.157-170
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• 2008

Purpose: This prospective study was conducted to reveal the haematological index change by low level radiation exposure in radiological environment our hospital workers. Materials and Method: We gathered the cumulative dose by Thermoluminenscent Dosimeters (TLD) over 9-yr period and examined hematological index counts change (RBC, Hb, Platelet, WBC, Monocyte, Lymphocyte, Neutrophilic, Basophilic, Eosinophilic) both occupational workers and controls. Of a total 370 occupational workers and 335 controls were compared. Results: This analysis has led to the following general observations 1) The average cumulative dose in male and female were $9.65{\pm}15.2\;mSv$, $4.82{\pm}5.55\;mSv$ respectively. 2) In both male and female, there were very low relationship between occupation period and cumulative dose (r< ${\pm}0.25$). 3) Occupation period was more increased, in male, WBC counts decreased and increased workers, RBC counts decreased workers were more than controls group (p<0.05). In female, WBC counts decreased and increased workers and W-eosino counts decreased workers were more than controls group (p<0.01). 4) Cumulative dose was more increased, in male, W-Lympho counts decreased workers and Platelet counts deceased workers were more than controls group (p<0.05). In female, W-lympho counts decreased workers and RBC counts decreased workers were more than controls group (p<0.05). Conclusions: We can find some kinds of blood index abnormal distribution in occupational radiation workers by comparing with controls. Occupational workers cannot avoid radiation exposure, in spite of the control it. Actually low level radiation adverse effect occurred not dose but probability. So workers must always try to reduce exposure by ourselves, furthermore as long as possible the government should provide rapidly that national system on radiation control for worker's health.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.4
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• pp.298-310
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• 1997

The aim of this research was to evaluate the biotic environments based upon the community pattern of demersal fishes in the Masan bay which is heavily polluted area in the southern part of Korea. The baited traps or bottom gillnet sampled bottom fishes at four sites totally 14 tunes from March 1994 to December 1996 with 2~3 months interval. The baited trap caught 0~ 10 species which were mainly starfish, crabs, conger eel, rockfish, or blenny with 0~50 individuals per a trap. Small crab(Atergatis sp.) was significantly dominant in the S1 site, most heavily polluted area, and starfish(Asterias sp. and/or Asterina sp.) in the other three sites. There was no animals at the S1 site in the March and July in 1994 and August in 1996. The gillnets caught 0~7 species which were mainly mantis shrimp, jellyfish, flounder, starfish, crabs, or sea weeds with 0~30 individuals per a pannel of bottom gillnet. Jellyfish was significantly dominant in the S1 site and mantis shrimp in the all four sites. There was no animals in the July 1994 at S1 site and in the August and December 1996 at S4 site. But at the August in 1995 or 1996 in the S1 site, the bottom gillnets caught mackerel, anchovy, gizzard-shad or mullet which was dead all. The species diversity index of samlings by trap or gillnet showed less than 1.0 in the 80% of the totally 56 samplings. This means that the stability of demersal fishes' community is very low owing to the poor biotic environments. But most of fishes living at Masan bay appeared well fitting with the heavily polluted environments. And some fishes, like mackerel, anchovy, gizzard-shad or mullet migrated into polluted Masan bay with the seasonal or annual fluctuation.