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http://dx.doi.org/10.5762/KAIS.2018.19.9.116

Association with Kinetic Characteristics of sperm in Duroc Boar and the Zygote Arrest 1 gene Polymorphism (g.2540T>C)  

Lee, Mi Jin (National Institute of Animal Science, RDA)
Ko, Jun Ho (National Institute of Animal Science, RDA)
Cho, Kyu Ho (National Institute of Animal Science, RDA)
Choi, Tae Jeong (National Institute of Animal Science, RDA)
Kim, Yong Min (National Institute of Animal Science, RDA)
Kim, Young Sin (National Institute of Animal Science, RDA)
Jin, Dong Il (Department of Animal Science & Biotechnology, Chungnam National University)
Cho, Eun seok (National Institute of Animal Science, RDA)
Kim, Nam Hyung (College of Animal Resource Sciences, Chungbuk National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.19, no.9, 2018 , pp. 116-123 More about this Journal
Abstract
The sperm quality is determined by the kinetic characteristics and acrosome integrity of the sperm. In the previous studies, analysis of semen quality had large errors because those experiments by using microscope had been conducted by people. In recent years, the molecular biological methods have been newly developed to complement the previous techniques. The ZAR1 gene is known to be a gene that affects early embryonic development in vertebrates, but there is no study of the association with semen. In this study, we analyzed the association between the kinetic characteristics and ZAR1 single nucleotide polymorphism (SNP) genotype. To detect the SNPs, we performed sequencing using genomic DNA from the whole bloods of Duroc pigs. We identified an SNP in the ZAR1 gene g.2540T>C. ZAR1 SNP genotypeing in 105 pigs revealed that the major and minor alleles were T and C, respectively. After we analyzed the association between the kinetic characteristics of sperm and the ZAR1 SNP genotype, we found a significant association in MOT (p<0.01), VSL (p<0.05) of the kinetic characteristics in the Duroc boars. It was confirmed that the boars with T allele were lower in MOT and VSL than C allele. Therefore, pigs with C allele are judged to be better at the MOT and VSL of semen. Based on these results, ZAR1 SNP genotyping may be a useful molecular biomarker to improve semen quality by applying molecular breeding technology.
Keywords
Boar; Candidate gene; Kinetic characteristic; Polymorphism; Zygote arret 1;
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