• The Science & Technology
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• v.31 no.3 s.346
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• pp.80-84
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• 1998

생물산업(생명과학기술)은 정보산업과 함께 21세기를 주도할 대 산업중의 하나이다. 지난 수십년간 급속히 발달한 분자생물학과 유전공학에 힘입어 생명과학기술은 폭발적으로 팽창해왔다. 생명과학기술은 그동안 인슐린을 비롯해 성장호르몬, 혈약응고인자 등 세상을 놀라게 했다. '인간복제'를 둘러싸고 전 세계를 떠들썩하게 했던 이 생명과학기술에 새 세기엔 어떻게 펼쳐질 것인가 알아본다.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.78-78
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• 2018

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.188-188
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• 2004

근래에 많은 형질전환 실험동물이 생산되어 인간을 대체하여 전임상실험에 사용되고 있으며, 그 요구는 점차 증가되고 있다. 그러나 지금까지 생산된 것은 소형 실험동물에 국한되고 있어, 사람과의 그 크기와 기능에 거리가 있었던 것이 사실이다. 본 실험은 중형 실험동물이자 사람과 여러 기관 및 조직이 유사하다고 알려진 돼지로부터 난자를 채취하여 난자의 핵을 GFP가 발현된 세포의 핵으로 치환하여 태어난 산자를 재복제하여 생산된 돼지 신생자를 이용하여 각 기관 및 조직에서 GFP의 발현을 RT-PCR, Northern blot 및 immunocytochemistry 방법으로 분석하였다. (중략)

• Journal of Apiculture
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• v.34 no.1
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• pp.27-37
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• 2019

Rapid detection of Tropilaelaps, an external parasite of honeybees that lead to malformation of honeybee or colony collapse disorder, is becoming important. But it is very difficult to find with the naked eye of Tropilaelaps. In this study, we have developed a method to detect the specific gene of Tropilaelaps from the hive debris and to know the number of Tropilaelaps in the hive through Tropilaelaps-specific quantitative detection. Tropilaelaps-specific gene amplified in DNA extracted from hive debris by consecutive PCR (1st detection, 2nd nested PCR). It could detect 101 molecules level of Tropilaelaps-specific gene and confirm the amplification of the Tropilaelaps-specific gene. It was possible to accurately quantify the number of Tropilaelaps from the hive debris sample, which is difficult to discriminate the presence of Tropilaelaps visually, through Tropilaelaps-specific detection. Under the microscope, Tropilaelaps was collected and quantitative detection of Tropilaelaps-specific genes was performed. It was possible to quantify the number of Tropilaelaps present in the hive through the molecules of the quantified Tropilaelaps-specific genes. We suggest that hive debris can represent as a micro-environment to hive and show that it can be a simpler and more accurate sample than using a parasitic host honeybee. We expect that hive debris should facilitate the monitoring of Tropilaelaps in hive.

• Journal of Apiculture
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• v.34 no.1
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• pp.39-46
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• 2019

BQCV multi-point PCR was developed as a rapid multiplex detection method for BQCV, one of the viral pathogens of honeybees. It could detect BQCV specific genes qualitative as well as quantitative detection based on ultra-rapid PCR. Three primer pairs (RNA dependent RNA polymerase, capsid protein, 3C like protease) were specifically designed for accurate the detection and were optimized for minimizing the detection time and increasing the sensitivity. Our advanced diagnostic system have the accuracy by lowering the concern about the variation in the BQCV detection site. In addition, it should be an opportunity to identify mutations that are mixed with other viruses.

• Journal of Soil and Groundwater Environment
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• v.27 no.4
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• pp.37-48
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• 2022

Accurate analysis of petroleum hydrocarbons in soil is an important prerequisite for proper source tracking of contamination. Identification of petroleum compounds is commonly carried out by peak pattern matching in gas chromatography. However, this method has several technical limitations, especially when the soils underwent biological, physical and chemical transformation. For instance, it is very difficult to distinguish jet fuel (JP-8) from kerosene because JP-8 is derivatized from secondary reaction between chemical agents (e.g. anti-oxidants, antifreezer and so on) and kerosene. In this study, an alternative method to separately analyze JP-8 and kerosene in the petroleum hydrocarbon contaminated soil was proposed. Qualitative analyses were performed for representative phenolic antioxidants [2,6-di-tert-butyl phenol (2,6-DTBP), 2,4-di-tert- butylphenol(2,4-DTBP), 2,6-di-tert-butyl-4-methyl phenol (2,6-DTBMP)] using a two dimensional gas chromatograph mass spectrometer (2D GC×GC-TOF-MS). This qualitative analysis of antioxidants in soil would be a useful complementary tool for the peak pattern matching method to identify JP-8 contamination in soil.

• Journal of Information Management
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• v.36 no.2
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• pp.125-151
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• 2005

The eight types of Korean bibliographic information of biology and life science are included in PubMed that serves universal medical bibliographic information. Other domestic bibliographic information are served on its own format at different organizations. To fulfill the CCBB homepage user's request that it is necessary that integration of bibliographic service for quick acquisition of research information, we intends to construct metadata registry and service about bibliographic information of biology and life science. This paper describes the design and implementation of bibliographic information network prototype for biology and life science that can be used to share latest research result, seminar presentation data, research note, and research paper etc as well as to exchange information between researchers through Open Archiving Community.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.169-169
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.41-41
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.115-115
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• 2019