• 제목/요약/키워드: 생리적 대사

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수온 변화와 개체크기에 대한 바지락, Ruditapes philippinarum의 최적 성장에 대한 연구

  • 이순우;왕순영;한경남
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.305-306
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    • 2001
  • 수온은 개체들의 활성도와 분포를 제한하는 직접적인 요인이며, 에너지 수지에 포함되는 섭이 활동, 대사작용과 성장들을 포함한 대부분의 생리학적 과정들에 영향을 미친다(Fry, 1971). 적온 범위에 대한 변통은 종간 뿐만 아니라 종내에서도 크다. 그러므로 특정한 조건하에 생태적으로나 상업적으로 가치가 있는 종들에 대한 성장 실험(Scope for growth)은 필요하며, SFG는 넓은 범위의 환경상태에 대해서 민감하고, 정량적이며 통합적인 스트레스에 대한 반응을 제공한다. (중략)

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Strontium Metabolism in Higher Plants: Effect of Strontium of the Polyamine Biosynthesis during Germination of Wheat(Triticum aestivum L.) (식물체 내에서 Strontium의 대사 : 밀(Triticum aestivum L.)의 발아과정중 Polyamine 생합성에 미치는 Strontium의 영향)

  • Kim, Tae-Wan;Heinrich, Georg.
    • Korean Journal of Environmental Agriculture
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    • v.14 no.1
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    • pp.55-71
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    • 1995
  • Wheat (Triticum aestivum L.) seeds were used to study a possible relationship between strontium and polyamines (PAs) in the coleoptile, root, and endosperm during germination. When $Sr^{2+}$ (0.001 mM + 10 mM) was applied to the incubation medium with $10\;{\mu}M$ $GA_3$, great increases in putrescine (Put) were observed in root and spermidine (Spd) in the coleoptile, depending on the concentration. In germinating seeds, putrescine accumulation was induced even at a low concentration (0.01 mM Sr), whereas spermidine accumulation was stimulated considerably at a high concentration (10 mM Sr). The putrescine levels, on a gram fresh weight (g-fr-wt) basis, in the roots which were growth-inhibited by 1 and 10 mM $Sr^{2+}$ were 22.4 and 15.3 fold higher respectively than at the same concentrations of $Ca^{2+}$. The accumulation of total polyamine (TA), in particular Put and Spd, induced by Sr seemed to be an important physiological response not only on a g fr wt basis but also on an RNA basis. In contrast, the levels of agmatine (Agm) and cadaverine (Cad) were notably enhanced by 10 mM $Ca^{2+}$ in the coleoptile and root. Cadaverine was detected only in $Ca^{2+}-treated$ seedlings. However, $Ca^{2+}-treatment$ in the range of 0.001 mM to 1.0 mM resulted in reduction of TA content. The distinction of accumulated polyamines and the change in diamine (DA) / TA and tri- and tertiary (tPA) / TA ratios were likely to be a physiological difference between $Sr^{2+}$ and $Ca^{2+}$ during germination in wheat.

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Imitation of Phosphoenolpyruvate to Oxaloacetate Pathway Regulation of Rumen Bacteria in Enteric Escherichia coli and Effect on C4 Metabolism (반추위 미생물이 가진 Phosphoenolpyruvate에서 Oxaloacetate 경로 조절기작의 대장균에서의 모사와 C4대사의 영향)

  • Kwon Yeong-Deok;Kwon Oh-Hee;Lee Heung-Shick;Kim Pil
    • Microbiology and Biotechnology Letters
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    • v.34 no.1
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    • pp.35-39
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    • 2006
  • One of the fermentative metabolism of enteric Escherichia coli was imitated after rumen bacteria, which have high C4 metabolism. E. coli expresses phosphenolpyruvate carboxylase (PPC) for the pathway between phosphoenolpyruvate (PEP) and oxaloacetate (OAA) during glycolytic condition while expresses phosphoenolpyruvate carboxykinase (PCK) during gluconeogenic condition. In contrast to enteric E. coli, rumen bacteria express the PEP-OAA pathway only by PCK. To verify the effect of the regulation imitation on the C4 metabolism of E. coli, PPC-deficient E. coli strain with PCK expression in glycolytic condition was constructed. The PEP-OAA regulation modified E. coli strain increased 2.5-folds higher C4 metabolite than the wild type strain. The potential use of C4 metabolism by regulation control is discussed.

Major Metabolites Involved in Skin Blackening of 'Niitaka' Pear Stored under Cold Temperature (신고 배 저온 저장 중 발생하는 과피 흑변에 관여하는 주요 대사체)

  • Lee, Eun Jin
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.359-365
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    • 2014
  • Oriental pear (Pyrus communis L. cv 'Niitaka') was stored at $0^{\circ}C$ for 5 months and major metabolites involved in blackening of the peel were analyzed by untargeted GC-MS and targeted HPLC methods. In this study, peels of sound and skin-blackened pears were analyzed and compared. Skin-blackened fruit was clearly characterized by a distinctive pattern in changes which included a decrease of malic acid, succinic acid, and ascorbic acid, while an increase of fumaric acid, threonine, and gluconic acid, which indicated both reduced metabolic activity and anti-oxidative capacity of the cells. Chlorogenic acid was a major phenolic compound and the peel of sound fruit showed high levels of free phenolic compounds compared than the peel of skin-blackened fruit which are believed to be related to oxidation of phenolics in skin-blackened tissue. The changes or profiling of major metabolites by targeted or untargeted analysis method could become a useful tool for understanding physiology, disorder mechanism, and identifying metabolic networks connecting primary and secondary metabolism in postharvest research.

참치정소로부터 핵산복합물질의 추출 및 생리활성 연구

  • 신종헌;송주영;권오건;이승용;김병각;이은방
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.74-75
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    • 2000
  • 핵산의 생합성은 salvage합성이 우선되며 이 경우 섭취된 음식물유래의 핵산등이 이용되므로 섭취되는 핵산의 영양학적 가치는 매우 높다(송영정사, 1995). Van Buren이나 Kulkarni등은 mouse에의 핵산 경구투여가 면역능을 개선하여 세균감염에 대한 방어에 유효하다고 보고하였고(Van Buren C.T et al, 1983, Kulkarni A.D et a 1986), 이들의 연구가 계기가 되어 섭취 핵산의 유용성에 대한 연구보고가 계속적으로 행하여지고 있는데 그 생리활성을 살펴보면 세포부활작용(Matsunaga et al, 1987) 면역증강작용(Frederic B. Rudolph, 1994) 과산화지질 형성억제작용(Uauy R et al 1985), 장내균총 및 지질대사의 개선작용(Uauy R, 1994, Angel Gil et al, 1986)등이다 본 연구에서는 부산물로 폐기되어지는 참치정소를 이용하여 핵산복합물질을 분리·정제하는 방법 및 그 추출물의 생리활성을 연구하므로써 새로운 기능성 소재로서의 이용가능성을 탐색하였다. (중략)

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Biofunctional Activities of Citrus Flavonoids (감귤류 플라보노이드의 생리기능 활성)

  • Cha, Jae-Young;Cho, Young-Su
    • Applied Biological Chemistry
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    • v.44 no.2
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    • pp.122-128
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    • 2001
  • This review showed a discussion on the biofunctional activities of citrus flavonoids. The major flavonoids of citrus species, hesperidin, hesperetin, naringin, and naringenin, were selected to evaluate their biological effects on the lipid metabolism in rats and hamsters, the proliferation of human hepatocyte HepG2 cells, and the antioxidative effect in lipid peroxidation models. These flavonoids showed hypotriglyceridemic effect in hamsters and hypochloesterolemic effect in rats. They also significantly inhibited the activities of phosphatidate phophohydrolase and acyl-CoA: cholesterol acyltransferase, which are key enzymes for biosynthesis of triglyceride and cholesterol, repectively, in vivo and in vitro experiments. These biofunctional activities by citrus flavonoids were shown more potent in the aglycone flavonoids, hespreretin and naringenin, than their corresponding glycoside flavonoids, hesperidin and naringin. These aglycone flavonoids also have inhibitory effects on proliferation of human hepatocyte cancer HepG2 cells. Hesperidin showed lowering activities of cellular triglyceride and cholesterol concentrations in HepG2 cells. Citrus flavonoids have significant importance in functional food industry as biofunctional active ingredients.

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Daily Maximum Temperature Mapping in Complex Terrain by Applying "Overheating Index" (과열지수를 이용한 복잡지형의 일 최고기온분포 추정)

  • 정유란;정일빈;서형호;황범석
    • Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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    • 2002.11a
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    • pp.77-80
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    • 2002
  • 기온은 생물의 대사과정에 직접적인 영향을 끼침으로서 생장과 발육을 결정하는 중요한 환경요인이며, 특히 식물은 개체 및 군락 수준에서 기온의 일 변화, 계절변화, 혹은 영년 변화에 반응한다. 최근의 농업 및 삼림 생태계 연구는 기온을 비롯한 환경요인의 영향을 생리과정의 정량적 모의를 근거로 이해하고, 이를 넓은 지역으로 확대하여 다양한 시간적 주기로 예측하는 방향으로 나아가고 있다 (Chung et al., 2002).(중략)

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Study on White Rice Consumption and Metabolic Risk Factor in Korean Elderly: Focused on Muscle Mass (한국 노인의 쌀 섭취와 대사위험지표에 관한 연구: 근육량 중심으로)

  • Oh, Chorong;No, Jae-Kyung
    • Culinary science and hospitality research
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    • v.21 no.5
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    • pp.147-159
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    • 2015
  • White rice consumption, a staple food for the Korean influence the other food consumption and nutrition balance. The aim of this study was to investigate the association with rice consumption based dietary intake and muscle mass for the Korean elderly who are easily in mal-nutrition. A total of 1,433 subjects (658 male and 775 female) 60 years or older from the fifth Korea National Health and Nutritional Examination Survey 2010 participated in this study. One of the findings was that there was association white rice consumption and marital status (single/with/without spouse) as well as education for men. Other interesting finding was the member of highest white rice consumption group showed higher muscle mass in both sexes. The other finding was that the more white rice consumption group showed the less meat consumption as well as the less consumption of other grains, noodle & dumpling, and flours & bread in both sexes. We found the significant association between white rice consumption level and muscle mass and several metabolic syndrome related factors in the elderly. But the association with white rice consumption and glucose metabolism related factor had not shown consistently.

The Distribution of $Cd^2+$ and its Physiological Toxicity in Commelina communis L. (닭의장풀 내 $Cd^2+$의 분포와 생리적 독성)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.63-67
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    • 2000
  • The effect of $Cd^2+$ on chlorophyll content, water potentials, ion transport, photosynthesis, stomatal apertures and $Cd^2+$ accumulation of organs in Commelina communis was investigated. 3-weeks old Commelina. communis was transferred to and grown in Hoagland solution in the presence or absence of 5 mM $Cd^2+$ for 4 days. $Cd^2+$ was accumulated in all parts of the organs including leaves, roots and stem. The proximity from the root and the age of leaf were significant factors responsible for the distribution of cadmium. Most of $Cd^2+$ was accumulated in the first leaf which was the nearest from the root. $Cd^2+$) accumulation in the leaves led to significant reductions in a series of physiological metabolism. $Cd^2+$ reduced total chlorophyll content up to 70%, and changed chlorophyll a/b ratio to 2. $Cd^2+$ also reduced about 20% of water potential. The treatment of $Cd^2+$ showed about 60% inhibition of photosynthetic activity when measured at various light intensity (100-1,000 $\mu$mol $Em^-2s^-1$). Similar effect was found in terms of stomatal conductance. Therefore, it could be concluded that the treatment of $Cd^2+$ decrease or block various physiological activities. [Cadmium, Photosynthesis, Stomatal conductance].

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Starter Culture for the Meat Fermentation

  • Kim, Seung-Hwa;Min, Sang-Gi
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 1997.06a
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    • pp.40-49
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    • 1997
  • 육제품 제조용 starter culture에 대한 연구는 기초과학을 바탕으로 관련 미생물의 생리대사 특성은 물론 이들의 환경적응성이나 상업적인 생산공정개발에 이르기까지 상당한 발전을 거듭해 왔다. 특히, 최근에 와서 유전공학의 응용으로 starter균주의 대사능력을 조절할 수 있고 생물학적 안전성을 쉽게 검증할수 있을 뿐만 아니라 원하는 활력을 안전하게 보존할 수 있도록 하는 연구도 진행되고 있다. 미생물의 생육환경인 육의 발효과정에 대한 연구를 통하여 육제품 starter culture개발의 근간이 되는 지식을 얻게 되었으며 어떤 요인이 발효과정중 starter 균주의 대사능력과 성장에 영향을 주는지 또한 발효과정중 starter culture간의 상호작용 및 starter와 윈래 혼합육에 존재하는 균들사이에서 일어나는 상호작용에 관한연구, 나아가서는 Bacteriocine과 Bacteriophage가 육제품제조에서 어떤 의미를 갖고 있는지 등에 관한 연구도 활발히 이루어지고 있다. 특히 이런 연구결과들을 통하여 이들 starter의 실제 상업적인 이용에 있어 원료육이나 부재료, 향신료의 선정은 물론 제조 공정을 제어하여 제품의 안전성을 기대할 수 있을 뿐만아니라 하나의 starter culture로 여러 종류의 발효sausage를 제조하여도 제품마다 최상의 능력을 발휘할 수 있는 새로운 균주의 개발에도 기여할 수 있다. 스타타컬쳐에 대한 연구상황은 균주별로 다르나 유산균에 대하여 가장 많이 연구되어 있고 Micrococci에 대한 연구도 상당히 진전되어 있다. 특히할만한 점은 아시아의 발효생선이 육제품 발효에 이용되는 Staphylococcus carnosus종의 habitat로 밝혀진 것이다. 뿐만 아니라 곰팡이 균주도 실제 praxis에 적합하게 개발시킬수 있다. 따라서 앞으로 발효육제품제조에 있어 starter culture가 갖는 의미는 매우 중요하며 특히 짧은 숙성기간을 거치는 발효소시지의 제조에 있어서는 필수불가결한 공정의 한 분야로 자리잡게 될 것이다.

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