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http://dx.doi.org/10.7235/hort.2014.13191

Major Metabolites Involved in Skin Blackening of 'Niitaka' Pear Stored under Cold Temperature  

Lee, Eun Jin (Department of Horticultural Science and Biotechnology, Seoul National University)
Publication Information
Horticultural Science & Technology / v.32, no.3, 2014 , pp. 359-365 More about this Journal
Abstract
Oriental pear (Pyrus communis L. cv 'Niitaka') was stored at $0^{\circ}C$ for 5 months and major metabolites involved in blackening of the peel were analyzed by untargeted GC-MS and targeted HPLC methods. In this study, peels of sound and skin-blackened pears were analyzed and compared. Skin-blackened fruit was clearly characterized by a distinctive pattern in changes which included a decrease of malic acid, succinic acid, and ascorbic acid, while an increase of fumaric acid, threonine, and gluconic acid, which indicated both reduced metabolic activity and anti-oxidative capacity of the cells. Chlorogenic acid was a major phenolic compound and the peel of sound fruit showed high levels of free phenolic compounds compared than the peel of skin-blackened fruit which are believed to be related to oxidation of phenolics in skin-blackened tissue. The changes or profiling of major metabolites by targeted or untargeted analysis method could become a useful tool for understanding physiology, disorder mechanism, and identifying metabolic networks connecting primary and secondary metabolism in postharvest research.
Keywords
discoloration; GC-MS; physiological disorder; storage;
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