• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.117-122
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• 2015

Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

• Journal of KSNVE
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• v.5 no.3
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• pp.275-283
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• 1995

본 글에서는 지능구조물의 개념설명과 더불어 ERF의 특성, ERF를 함유란 함유 한 지능구조물 시스템의 구성, 동적 모델링과 진동제어 그리고 그 응용성에 관한 연구 현황과 방향에 대해 살펴보았다. 설명한 바와 같이 지능구조물은 새로운 차원의 신생 하는 첨단분야로서, 소음 및 진동에 관련된 무한한 잠재력과 다양한 응용성으로 미루 어 볼때 아주 매력적인 연구 분야이다. 그러나, 여러 응용 시스템의 상품화 단계로의 도약에 있어서 각 시스템 구성 요소 분야별 해결해야할 연구 사항들이 있다. 먼저, 액추에이팅을 수행하는 ERF 자체의 내구성 문제로서 고온에서 ERF의 효과 하락과 장시간 사용시 ERF에 의한 마멸, 고체 입자의 침전에 의한 초기 상태 불안정 등이 있다. 아울러 기존의 장치의 성능을 능가하기 위해 보다 큰 효과를 나타내는 새로운 차원의 ERF개발이 요구된다. 그리고 센서기술 분야에서는 호스트 재료에 보다 쉽게 결합이 되고 여러가지 형태의 요구조건을 만족시킬 수 있으며 외부 환경조건에 강건 하고 다양한 센서 개발이 요구된다. 또한, 보다 일번적인 동적 모델링을 통해 적용 시스템에 적합하고 강건한 제어기에 대한 연구가 진행되어야 한다. 마지막으로 능동 제어기를 실제로 구현하기 위한 호스트 재료 각 요소마다 센서의 설치, 페회로 피드백 시스템 장착, 상호간의 인터페이스 등의 기술 발전이 요구되며, 아울러 보다 효율적 인 시스템의 성능 특성을 실현할 수 있는 호스트 재료와 기계 메카니즘이 필요로 된다. 이상의 설명에서 알 수 있듯이 지능구조물에 대한 연구는 어느 한 분야에서만 아니라 기계, 전기전자, 토목, 물리, 재료과학 등 통합형식에 의한 접근 방향으로 추진되어야 할 것이다.서 세탁기의 진동 소음을 저감시키기 위해 진동 소음원에 대해 논술하고, 진동해석을 위해 컴퓨터 시뮬레이션 결과를 이용한 저진동 기술 개발에 대하여 기술하고자 한다.rotary piston)식 압축기는 약 20여년 전 부터 냉방용 압축기에서부터 널리 쓰이게 되었다. 약 10여년전부터 상용화 된 스크롤(scroll) 형 압축기도 현재 상대적으로 용량이 큰 가정용 냉방기를 중심으로 많이 쓰이고 있다. 스크류형 압축기는 보통 중대형 상업용에 주로 쓰인다. 해결하려 하였고, 수치해석은 피스톤의 운동을 배제한 단순화한 흡배기계의 정상상태 유동해석이 주를 이루어왔다. Taghaui and Dupont 등[5]은 KIVA코드를 사용하여 흡기포트와 연소실 그리고 밸브의 움직임을 동시에 고려한 수치해석을 도입하였다. 하지만 이들이 밸브의 운동을 고려하기 위해 사용한 이동격자는 격자점은 시간에 따라 변화하지만 그 격자의 수가 일정하게 유지되어 있어서 밸브의 완전개폐를 해석할 수가 없다. 강희정[6]은 단일 실린더와 단일 배기밸브를 갖는 문제로 단순화하여 피스톤과 밸브의 움직임을 고려하므로써 배기행정 후 소음이 어떻게 전파해 나가는가를 연구하였다. 본 연구에서도 최소밸브간격과 최대밸브간격 사이에서만 계산이 가능하나 흡기의 경우는 밸브가 닫힐 때 생기는 압력파가 중요하므로 실린더와 밸브사이에 벽면조건을 주어 밸브의 개폐를 모사하였다.술을 보유하고자 한다. 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS.

• The Journal of the Korea Contents Association
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• v.20 no.8
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• pp.499-512
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• 2020

The development of the economy and network environment has a great impact on physical stores, and physical bookstores are gradually upgrading and transforming with the pace of the times. This study takes the modern bookstore under the concept of the third space as the research object. The purpose of it is to understand the needs of consumers for the space of the modern bookstores, explore the characteristics and development direction of them, which provides theoretical guidance for the follow-up development of the modern bookstore. Based on the concept of the third space and the theory of lifestyle shops and reorganizing theoretical investigation and prior research, the researcher extracts the characteristic elements of modern bookstores in line with the concept of the third space. Moreover, the research analyzes five bookstores opened in large-scale commercial facilities after 2010 as research cases. Through the analysis of the results, it shows that the modern bookstore is a multi-functional and compounded space which is gradually transformed from a single bookseller to a seller of selling lifestyle proposal. And through the analysis of big data to predict the changes in market consumption, we can find out the different needs of consumers, so as to carry out the targeted design of bookstore space and then improve the value of it. In the context of the co-development of economy and culture, the transformation of modern bookstores actually conforms to the change of consumer demand and realizes a virtuous circle.

• Journal of Korean Ophthalmic Optics Society
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• v.4 no.2
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• pp.129-133
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• 1999

For the myopia eyes of the people which are ametropia, the classified distribution has showed % for the simple myopic, 50% for the compound myopic astigmatism, 15% for the simple myopic astigmatism and 19% for the mixed astigmatism. The myopic ametropia for the both eyes has the distribution of 35% for -0.50D~-2.00Dptr, 54% for -2.00~6.00Dptr, and 11% for over -6.00Dptr. The classifying distribution for the age for the myopic ametropia was 54% for 15~20, 22% for 21~40, 14% for 41~60 and 10% for 61. The occupational distribution for the myopic ametropia has showed 61.5% for the student(Middle, High, College), 13.5% for the office worker, and 15% for the house wives as well as the small business. The hour affecting the refraction most for a day was after P.M. 7 which recorded 45% as the highest value. The reason is that the myopia degree decreases in the morning as the cornea flats and the situation is reversed in the afternoon so that there is a difference of Sph -0.50D and as getting darker the refraction degree of the light coming through the enlarged pupil refraction around the cornea is high. For the seasons the highest myopic degree has been recorded for 68% in the summer due to the shortage of nutrition and the climination inside the body by the exhaustion of sweat. In the blood types A and B are distributed closely as 34% and more active man with O has recorded higher myopic degree than woman. However woman showed higher accommodation power than man regardless the blood types. In the characteristic factors of myopic eye, the character feels fatigue easily has showed the distribution for 42% which is the highest and it could be classified largely by two the.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.28-34
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• 2006

A native extracellular acid phosphatase, phytase (EC 3.1.3.8), from Bacillus coagulans IDCC 1201 (commercially known as Lactobacillus sporogenes) used as probiotics, was characterized. Though some strains of B. coagulans have been evaluated with regard to several health-promoting effects, it has not been reported to produce phytase. Partially purified phytase front the strain IDCC 1201 had a pH optimum of 4.0 and a temperature optimum of $50^{\circ}C$, respectively. The requirement for divalent cations was studied and cobalt ion remarkably increased the enzyme activity. The removal of metal ions from the enzyme by EDTA decreased activity below 50%. The enzyme activity depleted restored when the assay was performed in the presence of $Co^{2+}$. Also, $Co^{2+}$ is the most active stimulator and has unique activation effect at high temperature. The phytase was specific for sodium phytate and p-nitrophenylphosphate, which is different from other known Bacilli phytases. The putative amino acid sequences of the phytase from B. coagulans IDCC 1201 were very similar to that of the phytase from B. subtilis strain 168. Based on these data, we concluded that the phytase from B. coagulans IDCC 1201 is a $Co^{2+}$-dependent acid phosphatase. Therefore, the strain B. coagulans IDCC 1201 is thought to be a valuable addititive for livestocks as well as a beneficial probiotics for human.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.297-307
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• 2017

There are many underground spaces available in an urban environment and they represent an important factor for efficient land use. Especially, underground shopping centers perform both physical and commercial functions at the same time and are frequently used as a means of achieving the revitalization of the city center. However, the underground shopping centers that were developed in the past sometimes led to recession due to their deterioration. This study was carried out for the purpose of proposing alternative methods for the (revitalization) of underground shopping centers. A survey was conducted on seven underground shopping centers in the Busan metropolitan area to understand the factors that determine their user satisfaction and future improvement. The survey data was analyzed using an ordered logit model. As a result of the analysis, service satisfaction, underground environment satisfaction and public facility satisfaction were selected as significant variables. It was concluded that it is necessary to take these factors into consideration, appropriately, when developing a new or renewing an existing underground shopping mall.

• Journal of Conservation Science
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• v.27 no.2
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• pp.201-209
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• 2011

While consolidants based on tetraethoxysilane (TEOS) have been widely used for the consolidation of decaying stone heritages, TEOS-based consolidants suffer from practical drawbacks, such as crack formation of the gel during the drying phase due to the developed capillary force. We have prepared new TEOS-based consolidants containing flexible (3-glycidoxypropyl) trimethoxysilane (GPTMS) in order to reduce capillary force development during gel drying. In this study, we have prepared TEOS/GPTMS-based consolidants containing ETEOS in order to improve the surface hydrophobisity. The physical properties of the TEOS/GPTMS/ETEOS solution with different hydrolysis were compared with those of the commercial products Wacker OH$^{(R)}$. The contact angle of the surface increased with the addition of the ETEOS, which is higher than that of Wacker OH$^{(R)}$. The sol-gel mechanism was manipulated by the degree of hydrolysis as well as the amount of ETEOS. The properties and the applicability of the developed consolidants for the decayed Korean granites are also investigated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.12
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• pp.6420-6426
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• 2013

The indoor areas for the commercial use of automatic monitoring systems of mobile robot localization improves the cognitive abilities and the needs of the environment with this emerging and existing mobile robot localization, and object recognition methods commonly around its great sensor are leveraged. On the other hand, there is a difficulty with a problem-solving self-location estimation in indoor mobile robots using only the sensors of the robot. Therefore, in this paper, a self-position estimation method for an enhanced and effective mobile robot is proposed using a marker and CCTV video that is already installed in the building. In particular, after recognizing a square mobile robot and the object from the input image, and the vertices were confirmed, the feature points of the marker were found, and marker recognition was then performed. First, a self-position estimation of the mobile robot was performed according to the relationship of the image marker and a coordinate transformation was performed. In particular, the estimation was converted to an absolute coordinate value based on CCTV information, such as robots and obstacles. The study results can be used to make a convenient self-position estimation of the robot in the indoor areas to verify the self-position estimation method of the mobile robot. In addition, experimental operation was performed based on the actual robot system.

• Journal of KIISE:Databases
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• v.30 no.3
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• pp.296-309
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• 2003

One of commercial applications of mobile computing is ATIS(Advanced Traveler Information Systems) in ITS(Intelligent Transport Systems). In ATIS, a primary mobile computing task is to compute the shortest path from the current location to the destination. In this paper, we have studied the shortest path re-computation problem that arises in the DRGS(Dynamic Route Guidance System) in ATIS where the cost of topological digital road map is frequently updated as traffic condition changes dynamically. Previously suggested methods either re-compute the shortest path from scratch or re-compute the shortest path just between the two end nodes of the edge where the cost change occurs. However, these methods we trivial in that they do not intelligently utilize the previously computed shortest path information. In this paper, we propose an efficient approximate shortest path re-computation method based on the dynamic window scheme. The proposed method re-computes an approximate shortest path very quickly by utilizing the previously computed shortest path information. We first show the theoretical analysis of our methods and then present an in-depth experimental performance analysis by implementing it on grid graphs as well as a real digital road map.

• Journal of the Korean Wood Science and Technology
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• v.16 no.2
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• pp.50-61
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• 1988

접착제(接着劑) 제조(製造)를 위하여 펄프폐액(廢液)중의 리그닌을 이용(利用)하는 연구(硏究)가 1930년경(年傾)부터 진행(進行)되여 아황산펄프폐액(廢液)중의 Lignosulfonate의 경우는 페놀수지(樹脂)나 요소수지(要素樹脂)에 증량(增量)시키거난 반응(反應)시키는 연구(硏究)인데 비하여 kraft 리그닌을 이용(利用)하는 경우는 페놀수지(樹脂)의 보문(報文)이 주류(主流)를 이루고 있으나 요소계(要素系) 공축합수지(共縮合樹脂)는 아직 발표(發表)된 바 없으며 또한 아직까지 리그닌을 이용(利用)한 상업적(商業的)인 접착제(接着劑)가 제조(製造)되어 활용(活用)되지 못하고 있는 실정(實情)이다. 따라서 본(本) 연구(硏究)는 합판제조(合板製造)의 원가절감(原價節減), 폐액이용(廢液利用)의 환경보존관점(環境保存觀點)에서 Krfat Pulp 폐액(廢液)중의 리그닌접착제(接着劑)의 제조(製造)하고져 실시(實施)하였으며, Urea-Lignin 접착제(接着劑)의 도포량(塗布量)은 일반적(一般的)으로 합판(合板)에 도포(塗布)되는 요소수지(要素樹脂)의 량(量)(320g/$m^2$)과 동일(同一)하고 압축응력(壓縮應力)은 균일(均一)하게 12kg/$cm^2$으로 하여 열압조건(熱壓條件)(온도(溫度), 시간(時間))의 영향(影響))을 아울러 규명(糾明)하였다. Urea-Formaldehyde와 Kraft lignin의 혼합비(混合比)는 중량비(重量比)(N.V.C)로 7:3으로 합성수지(合成樹脂)를 만들어 합판제조(合板製造)를 통(通)하여 구명(究明)를 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. 본(本) 실험(實驗)의 결과(結果) 요소수지(要素樹脂)의 약(約) 30%가 krfat lignin으로 대체(代替)할 수 있음을 보여준 바, Pulping 공정(工程)의 부산물(副産物)로 얻어진 폐액(廢液)은 합판제조시(合板製造時) 접착제(接着劑)의 대체원(代替源)으로 유효(有效)하다. 2. 경제적관점(經濟的觀點)에서 합판제조(合板製造)를 위한 최적열압조건(最適熱壓條件)은 $160^{\circ}C$에서 3분(分)으로 압착(壓搾)하는 것이 적절(適切)하였다.(상태(常態)): 16.49kg/$cm^2$, 내수(耐水) : 18.56kg/$cm^2$, 내온수(內溫水) : 12.53kg/$cm^2$). 3. 요소-리그닌 접착제(接着劑)로 제조(製造)된 합판(合板)의 전단인장강도(剪斷引長强度)는 양호(良好)한 접착력(接着力)을 나타냈으며, 내수접착력(耐水接着力)($30^{\circ}C$에서 3시간(時間) 심적후(沈積後) 시험(試驗)은 상태접착력(狀態接着力)과 별차이가 없거나 더 높은 인장강도력(引長强度力)을 보여준 바, 내수합판용(耐水合板用) 접착제(接着劑)로 상당한 전망(展望)을 나타냈다.