• Journal of Plant Biotechnology
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• 제43권4호
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• pp.473-478
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• 2016

With the increasing development and commercial use of genetically modified maize, it is essential to develop an appropriate method for detection of individual LMO (Living modified organism) events for monitoring the samples. In South Korea, commercial planting and accidental or unintentional releases of LMOs into the environment were not approved. In this study, to increase the efficiency of LMO detection, we developed simultaneous detection methods for 11 LM maize events. This multiplex PCR detection method is economical, as it saves time, cost and labor. We developed 11 individual LM maize events, and applied 4 multiplex PCR sets to the LM maize samples. These results are confirmed by applying the multiplex analysis of LMO environmental monitoring from 2012 to 2014, which represents the unintentionally released LM maize samples. The data were correlated with event specific PCR results. Our results indicate that the multiplex PCR method developed is suitable for detection of LM maize in LMO monitoring.

• Korean Journal of Remote Sensing
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• 제32권2호
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• pp.171-183
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• 2016

Land Surface Temperature (LST) retrieved from Landsat 8 measured from 2013 to 2014 and it is corrected by surface temperature observed from ground. LST maps are retrieved from Landsat 8 calculate using the linear regression function between raw Landsat 8 LST and ground surface temperature. Seasonal and annual LST maps developed an average LST from season to annual, respectively. While the higher LSTs distribute on the industrial and commercial area in urban, lower LSTs locate in surrounding rural, sea, river and high altitude mountain area over Seoul and surrounding area. In order to correct the LST, linear regression function calculate between Landsat 8 LST and ground surface temperature observed 3 Korea Meteorological Administration (KMA) synoptic stations (Seoul(ID: 108), Incheon(ID: 112) and Suwon(ID: 119)) on the Seoul and surrounding area. The slopes of regression function are 0.78 with all data and 0.88 with clear sky except 5 cloudy pixel data. And the original Landsat 8 LST have a correlation coefficient with 0.88 and Root Mean Square Error (RMSE) with $5.33^{\circ}C$. After LST correction, the LST have correlation coefficient with 0.98 and RMSE with $2.34^{\circ}C$ and the slope of regression equation improve the 0.95. Seasonal and annual LST maps represent from urban to rural area and from commercial to industrial region clearly. As a result, the Landsat 8 LST is more similar to the real state when corrected by surface temperature observed ground.

• Journal of the Korean Society of Food Science and Nutrition
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• 제36권12호
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• pp.1596-1603
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• 2007

This study was conducted to improve functional properties of salmon frame extracts using various commercial enzymes (Alkalase 2.4 L FG, Flavourzyme 500 MG, Neutrase 0.8 L and Protamex 1.5 MG). The ACE (angiotensin I converting enzyme) inhibitory activity was the highest ($IC_{50}=0.67mg/mL$) in the product incubated with Neutrase for 4 hrs (N4-treated hydrolysates) among the various extracts incubated with commercial enzymes for different times. However, antioxidant activities of all salmon frame extracts were less than 15%. There were no significant differences in the proximate composition and sensory evaluation of the fish odor and taste. However, N4-treated hydrolysate was improved in the extractive-nitrogen content and transmission compared to the other enzymatic hydrolysates. When compared to commercial Gomtang products, N4-treated hydrolysate was also high in protein, extractive-nitrogen, total amino acid, and calcium contents, while low in taste sensory score. There were no differences in transmission and sensory score on the fish odor between N4-treated hydrolysates and commercial Gomtang.

• Journal of Dairy Science and Biotechnology
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• 제38권2호
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• pp.89-98
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• 2020

The purpose of this study was to investigate the probiotic properties of lactic acid bacteria (LAB) isolated from a Korean traditional food kimchi. Gram staining was performed by Macrogen (Macrogen, Inc.) for identification of the LAB. Five strains of LAB were identified, including DKGF9 (Lactobacillus plantarum), DKGF1 (L. paracasei ), DKGF8 (L. casei ), DK207 (L. casei ), and DK211 (L. casei ). The biological activities of the isolated strains were assessed. The results showed that heat resistance of the strains was similar to or higher than the commercial strain L. acidophilus LA-5. Indirect testing of the ability of the strains to attach to the mucin layer revealed that DKGF9, DKGF1, and DKGF8 have high binding affinities for the mucous layer. All strains showed antimicrobial activity similar to or higher than the commercial strain LA-5. In proteolysis experiments, the diameters of proteolysis zones of the five strains increased in the period of 24-72 h, with DKGF1 exhibiting the largest zone diameter. Three strains were selected based on their antioxidant activities. Among the five isolated strains, L. paracasei DKGF1 showed potential probiotic activity, and thus, it may be useful for the development of health-promoting products.

• Journal of the Korean Society of Food Science and Nutrition
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• 제32권8호
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• pp.1245-1252
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• 2003

A starter formulation was developed to substitute a baker's yeast with natural starter when manufacturing bread products. To develop an active starlet, starter was formulated varying with types of wheat flours, level of water contents and various nutrients. Activities of starter were investigated in terms of viable counts of microbes and change of pH and total titratible acidity Domestic wheat flours contain 100 times more number of lactic acid bacteria than yeast regardless of types of wheat flours. The more protein contents in wheat flours, the more stable microbes in starter. This was considered to be the result of buffering effect of wheat proteins. The optimum level of protein content to ensure the activity of starter was more than 12.0%. Optimum level of water content in active starter was 110% based on strong flour. The more water or the less water had the tendency of decreasing viable counts of microbes. Addition of salt and sucrose had increased the activity of starters. However oligosaccharides did not affect the activity of starter. The optimum concentrations of salt and sucrose were 1.0% and 5.0% respectively. Bread with the starter was higher scored than breads with yeast in terms of all the quality and sensory characteristics except their volumes. In conclusion, a starter formulated with strong flour 100%, water 110%, salt 1% and sucrose 5% was considered to have high potential as a substitute of yeast in making natural bread.

• Journal of the Korean Institute of Landscape Architecture
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• 제49권1호
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• pp.42-53
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• 2021

Street trees improve the cityscape and air quality, reduce heat islands, and create wildlife habitats. Hence, they are essential parts of a city's green infrastructure. Therefore, several trees that are well adapted to the urban environment were planted. However, this caused the problem of simple trees being planted around the world. This study is to select more various street trees. To accomplish this, evaluation criteria and detailed indexes were created. The importance was indicated through the Analytic Hierarchy Process. For commercial roads, the priority of landscape characteristics is 0.2640, and among detailed indicators, the priority of shape is 0.1245. For work roads, the priority of landscape characteristics is 0.2496, and among detailed indicators, the priority of shape is 0.1177. For work roads, the priority of characteristics of civil service is 0.2250, and among detailed indicators, the priority of shape is 0.1177. For general roads, the priority of maintenance characteristics is 0.2479, and among detailed indicators, the priority of shape is 0.1062. For historical and cultural roads, the priority of regional characteristics is 0.3488, and among detailed indicators, the priority of regional characteristics is 0.1643. For ecological roads, the priority of ecosystem characteristics is 0.3488, and among detailed indicators, the priority of the diversity of species is 0.1643. For automotive-only roads, the priority of the ecosystem characteristics is 0.4639, and among detailed indicators, the priority of reducing emissions is 0.1643. This study will provide objective criteria for the selection of street trees.

• Food Science and Preservation
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• 제24권8호
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• pp.1168-1179
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• 2017

In this study, we tried to screen the Bacillus strain having safety probability by isolation of strains from traditional fermented food, measurement of probiotic properties, and the fermentative characteristics of Cheonggukjang. We isolated 400 Bacillus-like isolates from traditional fermented foods. Selected strains examined on the prevalent characteristic such as extracellular enzyme and antibacterial activities, and their safety probability was confirmed by biogenic amine productivity, hemolytic, and harmful substances and enzyme productivity. We selected the 5 strains by analysis of biogenic amine, antibacterial and B. cereus toxic associated gene. Five selected strains were examined on cell surface hydrophobicity, and bile and acid tolerance, and we selected the SRCM100730 as the final strain. SRCM100730 was confirmed B. amyloliquefaciens by 16S rRNA sequencing, and named the B. amyloloquefaciens SRCM100730 (KCCM11966P). Finally, we manufactured Cheonggukjang using SRCM100730 for confirmation of fermentation properties. Manufactured Cheonggukjang did not contain B. cereus, and showed that ${\gamma}$-PGA and extracellular enzyme activities were superior to commercial Chunggukjang. Amino nitrogen content was 544.02 mg% and 26 free amino acid were detected, and the bitterness-related amino acid content was lower than commercial Cheonggukjang. Especially, the amount of GABA was 3 fold higher than commercial Cheonggukjang. These results suggest that SRCM100730 have high availability in commercial probiotics market and fermented food industry.

• Korean Journal of Environmental Biology
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• 제41권2호
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• pp.109-125
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• 2023

This study was conducted to select target fish species as baseline research for accumulation analysis of major hazardous chemicals entering the aquatic ecosystem in Korea and to analyze the impact on fish community. The test bed was selected from a sewage treatment plant, which could directly confirm the impact of the inflow of harmful chemicals, and the Geum River estuary where harmful chemicals introduced into the water system were concentrated. A multivariable metric model was developed to select target candidate fish species for hazardous chemical analysis. Details consisted of seven metrics: (1) commercially useful metric, (2) top-carnivorous species metric, (3) pollution fish indicator metric, (4) tolerance fish metric, (5) common abundant metric, (6) sampling availability (collectability) metric, and (7) widely distributed fish metric. Based on seven metric models for candidate fish species, eight species were selected as target candidates. The co-occurring dominant fish with target candidates was tolerant (50%), indicating that the highest abundance of tolerant species could be used as a water pollution indicator. A multi-metric fish-based model analysis for aquatic ecosystem health evaluation showed that the ecosystem health was diagnosed as "bad conditions". Physicochemical water quality variables also influenced fish feeding and tolerance guild in the testbed. Eight water quality parameters appeared high at the T1 site, indicating a large impact of discharging water from the sewage treatment plant. T2 site showed massive algal bloom, with chlorophyll concentration about 15 times higher compared to the reference site.

• Journal of Korean Society of Environmental Engineers
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• 제32권7호
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• pp.656-664
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• 2010

We investigated the levels and distribution patterns of 16 priority polycyclic aromatic hydrocarbons (PAHs), 12 Coplanar polychlorinated biphenyls (Co-PCBs) and organochlorine pesticides (OCPs) in the surface sediments from the Nakdong river basin, Korea. The levels of 16 PAHs and 12 Co-PCBs in the river sediment samples ranged from not detected (N.D.)~969.3 ng/g-dry, 4.2~7716.5 pg/g-dry (0.0~10.1 pg-TEQ/g-dry), respectively. Also, the levels of 16 PAHs were from 5.8~2987.2 ng/g-dry and 4.3~461.1 pg/g-dry (0.0~0.6 pg-TEQ/g-dry) for 12 Co-PCBs in the lake sediment samples. Only dichloro-diphenyl-trichloroethane (DDT) were detected among target OCPs and the concentration ranged from N.D.~1.5 ng/g-dry in the river sediment samples. These contamination levels were far below the guideline values suggested for environmental quality assessment. Low molecular weight PAHs were dominant in the river sediment samples, while middle and high molecular weight PAHs were dominant among 16 PAHs in the lake sediment samples. PCB-118 and PCB-105 were predominant congeners in the sediment, which were similar to the results obtained from previous studies. With these results, the assessment of potential sources of PAHs and Co-PCBs contamination in the sediments of the Nakdong river basin was performed. The pyrogenic-PAHs originated from combustion of fossil fuel and biomass were related with the PAHs contribution in most of the sediment samples, and Co-PCBs in the sediment samples were related with commercial PCB products.

• Korean Journal of Environment and Ecology
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• 제24권2호
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• pp.166-177
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• 2010

This study is to identify potential factors to impact on appearance of wildbirds which live in the core forest around urban area in Gangdong-gu, Seoul. As a result of analysis of, studies on biotope showed most of urbanization biotope was biotope of residential areas with high green coverage and biotope of residential and business areas with low green coverage while most of biotope of green and openspace was core green biotope. The research area was divided into several blocks based on biotope types in the urbanization areas excluding green and openspace. As a result of research on wildbirds, total 51 species 3,419 individuals appeared in spring and total 35 species 4,213 individuals appeared in winter. 24 wild bird species were selected as subjects of this study among 31 species seen in urbanization areas, since urban birds, rapacious birds, waterside birds were excluded from the study for the proper consideration. Then this study looked at how many species and individuals of the subjects were observed at each research block in urbanization areas during spring and winter separately. Landuse structure and green structure in each block were examined to see whether these structures affect the number of wild birds observed in urbanization areas of Gangdong-gu. Furthermore, the distance between these blocks and green was assessed. While studying the potential links between the landuse structure and the number of wild birds observed in urbanization areas of Gangdong-gu, block area, green coverage, and building-to-land ratio were believed to affect the number of types and species of wild birds in the research area. In terms of correlation analysis of whether green structure affected the number of wild birds observed in urbanization areas of Gangdong-gu, crown volume of layers, the average green patch area, the average height of canopy layer were found to have an impact not only on the number of types but also species of wild birds in the research area.