• Title/Summary/Keyword: 살충

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Bioactivities of Korean Ginkgo (Ginkgo biloba L.) Extract and Its Potential as a Natural Pesticide (은행나무 추출물의 생물활성 및 천연물농약으로 이용 가능성)

  • Lee, Hyang-Burm;Kim, Han-Nah;Kim, Mi-Kyung;Kim, Chang-Jin;Kwon, Oh-Sung
    • Research in Plant Disease
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    • v.9 no.2
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    • pp.99-103
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    • 2003
  • Bioactivities of Korean ginkgo (Ginkgo biloba L.) extract were investigated against several fungi, general bacteria and insect pests. Crude methanolic extracts of different parts of Korean ginkgo showed different bioactivities depending on the target organisms. The methanolic extract showed in vitro antimicrobial activity at dose of 200 ug per paper disc. The extract of ginkgo stalk was some higher than seed coat and root. The extract also showed a remarkable in vivo antifungal activity against green mold (Trichoderma harzianum) on compost surface of spawn bags and in vivo insecticidal activity to Nilaparvata lugens, Plutella xylostella and Tetranychus urticae. This study suggests that Korean ginkgo extracts have a potential as a natural pesticide.

Insecticidal Effect of Dermatoohagoides pteronyssinus using Ginkgo biloba Leaves Extracts (은행잎 추출물의 집먼지 진드기 Dermatophagoides pteronyssinus에 대한 살충효과)

  • Lee, In-Hwa;Park, Ju-Young;Choi, Seung-Hyun
    • KSBB Journal
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    • v.22 no.1
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    • pp.58-61
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    • 2007
  • A Insecticidal effect of Ginkgo biloba leaves extract was conducted for Dermatophagoides pteronyssinus as a predominant species in korea. D. pteronyssinus has been cultured in constant temperature and humidity chamber at the 25$^{\circ}C$ and 75% of relative humidity. The mortality of mites was determined by counting the dead bodies for every hour within 48 hours with pin hall microscope after treated by Ginkgo biloba leaves extract spreaded on 0.1 g of mass cultured media. The sequence of mortality for D. pteronyssinus are as follows, bilobalide was 91.6%, 80$^{\circ}C$ water extract was 82.8%, second water fraction was 75%, ethyl acetate fraction from 80$^{\circ}C$ water fraction was 73%, first ethyl acetate fraction from 80$^{\circ}C$ water extract was 69.4%, putaltrin was 65%, distilled water was 58%, methanol extract was 57.8%, Ginkgolide-A was 57.1 %, ethyl acetate fraction of 80$^{\circ}C$ water extract was 55%, respectively. From the these results we conclude that the bilobalide is the most effective component in the Ginkgo biloba leaves extract having insecticidal effect on house dust mite.

Insecticidal and Acaricidal Activities of Plant Extracts (식물체 추출물의 살충 및 살비활성)

  • Kwon, Min;Lee, Seong-Baek;Ahn, Young-Joon;Park, No-Jung;Cho, Kwang-Yun
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.492-497
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    • 1994
  • Extracts of 43 species of plants were tested for their insecticidal and acaricidal activities against six species of insect pests and one mite species. The methanol extract of Ginkgo biloba leaves selectively was found to have potent insecticidal activity against Nilaparvata lugens (Stal), whereas steam distillate of Thujopsis dolabarata var. hondai sawdust showed potent insecticidal activities with a broad spectrum. The methanol extract of Pinus densiflora leaves and steam distillate of T. dolabrata var. hondai sawdust exhibited potent activities against Tetranychus urticae (Koch).

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Larvicidal and Antifeeding Activities of Oriental Medicinal Plant Extracts against Plutella xylostella and Spodoptera litura (배추좀나방과 담배거세미나방 유충에 대한 한방식물체 추출물의 살충 및 섭식저해활성)

  • Kwon, Hyung-Wook;Ahn, Young-Joon;Kweon, Jeong-Hyun;Lee, Sang-Gil;Byun, Byung-Ho
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.503-508
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    • 1994
  • Methanol extracts from 45 species of oriental medicinal plants belonging to 17 families were subjected to a screening test for their larvicidal and antifeeding activities against two species of lepidopteran larvae, diamondback moth (Plutella xylostella L.) and tobacco cutworm (Spodoptera litura F.) by a leaf-dipping method. At a concentration of 8,000 ppm, methanol extract of Corydalis turrschaninovii roots only was found to have potent larvicidal activity against S. litura. At 5,000 ppm, strong antifeeding activity against both larvae was observed from the extracts of Clerodendron trichotomum, C. trichotomum var. esculentum, Inura helenium, Arctium lappa, Artemisia messerschmidtiana, Anthriscus sylvestris, Angelica dahurica, and C. turrschaninovii.

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Toxicity of several insecticides to Dichromothrips smithi Zimmermann(Thysanoptera : Thripidae) (난총채벌레의 살충제 감수성)

  • Ahn, Ki-Su;Lee, Ki-Yeol;Kang, Hyu-Jung;Park, Sung-Kyu;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.6 no.4
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    • pp.244-249
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    • 2002
  • This study was carried out to investigate the toxicities of 22 insecticides to Dichromothrips smithi. Insecticidal activity was evaluated by testing systemic action and residual effect in laboratory. All insecticides used in this study did not affect on the egg of D. smithi, although organophosphates such as fenitrothion, fenthion, methidathion, phenthoate, and phenthoate+ethofenprox suppressed the egg hatchability completely. On D. smithi larva fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, thiamethoxam, abamectin, chlorfenapyr, emamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. On D. smithi adult fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, abamectin, emamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. Root-uptake systemic effects of phenthoate on the larva of D. smithi was 43.3%. Whereas, systemic effect of other insecticides was less than 20%. Insecticide with more than 80% residual effect for 7 days after treatment were fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, emamectin benzoate, fipronil, spinosad, and phenthoate.

Linear Free Energy Relationship on the Phosphorylation of Acetylcholinesterase by Insecticidal O,O-Diethylphenylphosphate Derivatives (살충성(殺蟲性) O,O-Diethylphenylphosphate유도체(誘導體)들에 의(依)한 Acetylcholinesterase의 Phosphorylation에 미치는 자유(自由)에너지 관계(關係))

  • Sung, Nack-Do
    • Korean Journal of Agricultural Science
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    • v.11 no.1
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    • pp.176-181
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    • 1984
  • Linear free energy relation ship(LFER) on the insecticidal activity of O,O-diethylphenylphosphate (A) and 3,5-dimethylphenyl-O,O-diethylphosphate (B) derivatives were studied by EHT MO calculation method and regression analysis method. LFER between varying substituent constants and $pI_{50}$ constants of phosphates, (A) & (B) were calculated with applying Hammett, Okamoto-Brown, Taft and Swain-Lupton's DSP equations;percent resonance effect(R) and field effect(F) of (A) were %R=33.5 & %F=66.5 and also that of (B) were %R=2 & %F=98, respectively. On the basis of above findings, the insecticidal activities were similar for both (A) and (B), but (B) have larger field and inductive contribution than (A), due to the 3,5-dimethyl group of (B).

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Studies on the Cholinesterase Inhibition and Toxicity of Various Organophosphorus Insecticides to the Hibernating Rice Stem Borer Larvae, Chilo suppressalis WALKER (이화명충에 대한 유기인살충제의 Cholinesterase 저해작용 및 살충력에 관하여)

  • Chang Chang Hyo;Saito Tetso;Iyatomi Kisabu
    • Korean journal of applied entomology
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    • v.10 no.1
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    • pp.13-22
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    • 1971
  • This experiment was conducted to investigate the differences of the in vitro inhibitory effect of various organephobphorua insecticides on the chlinesterase from rice stem borer and those of the toxicity of them against the insect, with special references to the relationship between the cholinesterase inhibition and the toxicity. The results obtained were summarized as follows: Phosphate compounds shelved stronger inhibitory effect on the cholinesterase than thhiophosphate compounds, but was not stronger in toxicity than the latter. Diethoxy compounds were not always stronger than dimethoxy in cholinesterase inhibition and the toxicity of organophosphorus insecticides. The organophosphorus insecticides that inhibited strongly the cholinesterase were not always stronger in the toxicity.

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Herbicidal and Insecticidal Potentials of 5-Aminolevulinic acid, a Biodegradable Substance (생분해성 생리활성물질 5-aminolevulinic acid의 제초 및 살충활성)

  • Chon, Sang-Uk
    • The Korean Journal of Pesticide Science
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    • v.11 no.1
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    • pp.52-58
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    • 2007
  • ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide and insecticide by the action of the protoporphyrinogen IX oxidase (Protox IX). The present study was conducted to determine growth responses of plant and insects to ALA, biodegradable biopesticidal substance. In the paddy condition experiment, plant height and shoot fresh weight of barnyardgrass (Echinochloa crus-galli) was more reduced by ALA than rice plants, even though both plant species show great phytotoxicity. Hairy crabgrass (Digitaria sanguinalis), a monocot weed, was more sensitive to ALA at 5mM under upland condition when ALA applied on the foliage, compared with soybean (Glycine max) as a dicot crop. ALA solutions were tested for their insecticidal and larvicidal activities against Spodaptera exigua (Hubner) and Tetranychus urticae Koch. by foliar application and leaf-dipping method. The result showed higher insecticidal activity of ALA at 10mM and its mixture with insecticide luferon against S. exigua. Strongest insecticidal activity against T. urticae was observed from the ALA solution at 10mM 72 days after application. This results show that ALA solution had potent herbicidal and insecticidal activities against agricultural pests even though their activities were lower than those of synthetic pesticides.

Contact and Residual Toxicities of 26 Insecticides Against the Cigarette Beetle, Lasioderma serricorne and the Maize Weevil, Sitophilus zeamais (권연벌레와 어리쌀바구미에 대한 26종 살충제의 접촉독성 및 잔효성)

  • Cho, Sun-Ran;Shin, Youn-Ho;Yoon, Chang-Mann;Kim, Gil-Ha
    • The Korean Journal of Pesticide Science
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    • v.15 no.3
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    • pp.310-316
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    • 2011
  • This study was performed to evaluate the contact toxicity and residual toxicity of the 26 commercially registered insecticides against cigarette beetle, Lasioderma serricorne and maize weevil, Sitophilus zeamais adult. Among 26 insecticides, seven insecticides (chlorpyrifos, chlorpyrifos-methyl, fenitrothion, fenthion, phenthoate, cypermethrin, ${\alpha}$-cyhalothrin) against L. serricorne adult and five insecticides (chlorpyrifos, chlorpyrifos-methyl, fenitrothion, fenthion, phenthoate) against S. zeamais adult showed perfect mortality, $LC_{50}$ (ppm) values of those selected insecticides were appeared lowest value on ${\alpha}$-cyhalothrin (1.46) against L. serricorne adult and chlorpyrifos-methyl (0.64) against S, zeamais adult, respectively. However, chlorpyrifos-methyl still remain high toxicity until 90th days after treatment against both L. serricorne and S. zeamais adults. From above results, it will be useful information to select insecticides effective against L. serricorne and S. zeamais adults.

Mosquitocidal Proteins from Escheriachia coli pSL 2-1 Clone and Bacillus sphaericus 1593 (Escheriachia coli pSL 2-1 클론과 Bacillus sphaericus 1593 균주가 생산한 모기치사 단백질)

  • Lee, Hong-Sup;Kim, Soo-Young;Lee, Hyung-Hoan
    • Microbiology and Biotechnology Letters
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    • v.16 no.5
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    • pp.389-392
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    • 1988
  • A clone pSL 2-1, which is a recombinant plasmid believed to contain the mosquitocidal crystal-line protein gene of the Bacillus sphaericus 1593, was expressed in Escherichia coli JM83 and the product of the clone was purified and identified. The unsolubilized mosquitocidal crystal proteins from the B. sphaericus had formed 43, 58, 64, 100, 113, and 130 Kd bands in the SDS-polyacrylamide gel, but the NaOH-solublized proteins at pH 12 formed 2 protein bands of 43- and 64Kd in the gel because the larger protein (precursor) bands were cleaved. The products of the pSL 2-1 clone was purified by Sephadex G-200 and only the fractions having lethal activity to the 3rd in-star larvae of mosquito Culex pipiens were analyzed by the gel. The only single protein band of 42 Kd toxic to the larvae was formed. The major toxic protein being produced from the B. sphaericus 1593 and the pSL 2-1 clone was found to be the 42 Kd.

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