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http://dx.doi.org/10.14578/jkfs.2015.104.3.512

Selection and Characterization of Bacillus licheniformis MH48 for the Biocontrol of Pine Wood Nematode (Bursaphelenchus xylophilus)  

Jeong, Min-Hae (Division of Applied Bioscience and Biotechnology, Chonnam National University)
Yang, Seo-Young (Division of Applied Bioscience and Biotechnology, Chonnam National University)
Lee, Yong-Sung (Division of Applied Bioscience and Biotechnology, Chonnam National University)
Ahn, Young-Sang (Division of Forest Resources, Chonnam National University)
Park, Yun-Serk (Purne Co., Ltd., Institute of Environmentally-Friendly Agriculture, Chonnam National University)
Han, Hye-rim (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Chonnam National University)
Publication Information
Journal of Korean Society of Forest Science / v.104, no.3, 2015 , pp. 512-518 More about this Journal
Abstract
Pine wilt disease (PWD) caused by pine wood nematode, Bursaphelenchus xylophilus, has become the most serious threat to pine trees in Korea. This study was subjected to investigate effective biological control agent against PWD. To select nematocidal bacteria against PWD, Bacillus licheniformis MH48 was selected among five bacteria due to its high nematocidal potential. B. licheniformis MH48 was tested for cell growth and protease activity to evaluate its nematicidal potential. In the B. licheniformis MH48, cell numbers were highest three days after incubation, while protease activity was highest after seven days. In the effect of different concentrations of B. licheniformis MH48 culture broth against B. xylophilus, 20% concentration of culture broth showed approximately 80% of pine wood nematode mortality compared to the control. Especially, pine wood nematode's cuticle layers were degraded two days after treatment of B. licheniformis MH48 culture broth. The present study suggests that B. licheniformis MH48 can be one of the potential biocontrol candidates against pine wood nematode due to its ability to produce protease.
Keywords
pine forest; pine wood nematode; Bursaphelenchus xylophilus; pine wilt disease; biological control; Bacillus licheniformis;
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1 Abo-Elyousr, K.A., Khan, Z., El-Morsi Award, M., and Abedel-Moneim, M.F. 2010. Evaluation of plant extracts and Pseudomonas spp. for control of root-knot nematode, Meloidogyne incognita on tomato. Nematropica 40(2): 289-299.
2 Choi, I.H., Park, J.D., Shin S.C., and Park, I.K. 2006. Nematicidal activity of medicinal plant extracts and two cinnamates isolated from Kaempferia galangal L. (proh Hom) against the pine wood nematode, Bursaphelenchus xylophilus. Nematology 8: 359-365.   DOI
3 Dong, J., Zhou, Y., Li, Ru., Zhou, Wei., Li, L., Zhu, Yangui, Huang, Rong, and Zhang, Keqin. 2006. New nematicidal azaphilones from the aquatic fungus Pseudohalonectria adversaria YMF1.01019. FEMS Microbiology Letters 264: 65-69.   DOI
4 Dwinell, L.D. and Nickle, W.R. 1989. An overview of the pinewood nematode ban in North America. General technical report SE-55, North America Forestry Commission Publication No. 2, Southeastern Forest Experimental Station, Forest service, United states Department of Agriculture. pp. 13.
5 Edward, O.R. and Linit, M.J. 1992. Transmission of Bursaphelenchus xylophilus through oviposition wounds of Monochamm carolinensis (Coleoptera: Cerambycidae). Journal of Nematology 24: 133-139.
6 Elbadri, G.A.A., Lee, D.W., Park, J.C., Yu, H.B., Choo, H.Y., Lee, S.M., and Lim, T.H. 2008. Nematicidal screening of essentials oils and herbal extracts against Bursaphelenchus xylophilus. Plant Pathology Journal 24: 178-182.   DOI
7 Gabriel P., Diogo N.P., Romeu F., Paula V., Susana S.S., Luis F., Isabel M.O.A., and Paula V.M. 2013. Nematicidal Bacteria Associated to Pindwood namatode produce extracellular proteases. PLoS Onel 8(11): e79705.   DOI
8 Hong, S.H., Anees, M., and Kim, K.Y. 2013. Biocontrol of Meloidogyne incognita inciting disease in tomato by using a mixed compost inoculated with Paenibacillus ehimensis RS820. Biocontrol Science and Technology 23: 1024-1039.   DOI
9 Kamata, N. 2008. Intergrated pest management of pine wilt disease in Japan: tactics and strategies. In: Zhao, B.G., K. Futai, J.R. Sutherland and Y. takeuchi(eds.). Pine wilt disease, Springer, Tokyo. pp. 304-322.
10 Kembhavi, A.A., Kulkarni, A., and Pant, A. 1993. Salt-tolerant and thermostable alkalien protease from Bacillus subtilis NCIM No. 64. Applied Biochemistry and Biotechnology 38: 83-92.   DOI
11 Korea Forest Service. 2014. Statistical yearbook of forestry. No.44 http://www.forest.go.kr (2015.01.11).
12 Khan, Z., Kim, S.G., Jeon, Y.H., Khan, H.U., Son, S.H., and Kim, Y.H. 2008. A plant growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode. Bioresource Technology 99: 3016-3023.   DOI
13 Kishi, Y. 1955. The pine wood nematode and the Japanese pine sawyer. Thomas Company, Tokyo, Japan. pp. 302.
14 Kong, J.O., Lee, S.M., Moon, Y.S., Lee, S.G. and Ahn, Y.J. 2007. Nematicidal activity of cassia and cinnamon oil compounds and related compounds toward Bursaphelenchus Lignicolus (Nematoda: Aphelenchoididae). Journal of Nematology 39: 31-36.
15 Kuroda, K. 1989. Terpenoids causing tracheid-cavitation in Pinus thunbergii infected by the pine wood nematode (Bursaphelenchus xylophilus). Annals of the Phytopathological Society of Japan 55: 170-178.   DOI
16 Lee, S.M., Chung, Y.J., Moon, Y.S., Lee, D.W., Choo, H.Y., and Lee, C.K. 2003. Insecticidal activity and fumigation conditions of several insecticides against Japanese pine sawyer (Monochamus alternatus) larvae. Journal of Korean Forestry Society 92: 191-198. (in Korean abstract)
17 Lee, S.M., Kim, D.S., Kim, C.S., Choo, H.Y., and Lee, D.W. 2008. Possibility of simultaneous control of pine wilt disease and Thecodiplosis japonensis and or Matsucoccus thunbergianae on black pine (Pinus thunbergii) by abamectin and emamectin benzoate. Korean Journal of Pesticide Science 12: 363-367. (in Korean abstract)
18 Lee, Y.S., Nguyen, X.H., Naing, K.W., Park, Y.S., and Kim. K.Y. 2015. Role of lytic enzymes secreted by Lysobacter capsici YS12115 in the control of root-knot nematode of tomato plants. Indian Journal of Microbiology 55(1): 74-80.   DOI
19 Li, W., Roberts, D.P., Derby, P.D., Meyer, S.L.F., Lohrke, S., Lumsden, R.D., and Hebbar, K.P. 2002. Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F. Crop Protection 21: 129-135.   DOI
20 Lee, Y.S. and Kim, K.Y. 2015. Antagonistic potential of Bacillus pumilus L1 against root-knot nematode, Meloidogyne arenaria. Journal of phytopathology doi: 10.111/jph.12421.
21 Mamiya, Y. and Enda, N. 1972. Transmission of Bursaphelenchus Lignicolus (Nematoda: Aphelenchoididae) By Monochamus Alternatus (Coleoptera: Cerambycidae). Nematologica 18: 159-162.   DOI
22 Morimoto, K. and Iwasaki, A. 1972. Role of Monachamus alternatus (Coleoptera: Cerambycidae) as a vector of Bursaphelenchus Lignicolus (Nematoda: Aphelenchoididae). Journal of Japanese Forestry 54: 177-183.
23 Nguyen, X.H., Naing, K.W., Lee, Y.S., Jung, W.J., Anees, M., and Kim, K.Y. 2013. Antagonistic potential of Paenibacillus elgii HOA73 agasint the root-knot nematode, Meloidogyne incognita. Nematology 15(8): 991-1000.   DOI
24 Oku, H., Shiraishi, T., Ouchi, S., Kurozumi, S., and Ohta, H. 1980. Pine wilt toxin, the metabolite of a bacterium associated with a nematode. Naturwissenschaften 67: 198-199.
25 Park, I.K., Kim, J.H., Lee, S.G., and Shin, S.C. 2007. Nematicidal activity of plant essential oils and components from Ajowan (Trachyspermum ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) essential oils against pine wood nematode (Bursaphelenchus Xylophilus). Journal of Nematology 39(3): 275-279.
26 Sasaki, S., Odani, K., Nishiyama, Y., and Hayashi, Y. 1984. Development and recovery of pine wilt disease studied by tracing ascending sap flow marked with water soluble strains. Journal of Japanese Forestry Society 66: 141-148.
27 Woo, S.M. and Kim, S.D. 2007. Confirmation of Non-Siderophore Antifungal Substance and Cellulase from Bacillus licheniformis K11 Containing Antagonistic Ability and Plant Growth Promoting Activity. Journal of Life Science 17(7): 983-989. (in Korean abstract)   DOI
28 Sela, S., Schickler, H., Chet, I., and Spigel, Y. 1998. Purification and characterization of Bacillus cereus collagenolytic/proteolytic enzyme and its effect on Meloidogyne jananica cuticular proteins. European Journal of Plant Pathology 104: 59-67.   DOI
29 Siddiqui, Z.A. and Mahmood, I. 1999. Role of bacteria in the management of plant parasitic nematodes: a review. Bioresource Technology 69: 167-179.   DOI
30 Takeuchi, Y. 2008. Host fate following infection by the pine wood nematode. In: Zhao, B.G., K. Futai, J.R. Sutherland and Y. takeuchi(eds.). Pine wilt disease, Springer, Japan. pp. 235-249.
31 Yang, J., Li, J., Liang, L., Tian, B., Zhang, Y., Cheng, C., and Zhang, K. 2007. Cloning and characterization of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys conoides. Archives of Microbiology 188: 168-174.
32 Yi, C.K., Byun, B.H., Park, J.D., Yang, S.I., and Chang, K.H. 1989. First finding of the pinewood nematode, Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle and its insect vector in Korea. Research Reports of the Forestry Research Institute. 38: 141-149. (in Korean abstract)
33 Yik, C.P. and Birchfield. W. 1981. Observations on the morphology of the pine wood nematode, Bursaphelenchus xylophilus. Journal of Nematology 13(3): 376-384.