• Title/Summary/Keyword: 살균제 활성

Search Result 143, Processing Time 0.028 seconds

Correlations Between In Vitro and In Vivo Methods for Screening Fungicides Against Corn Disease (옥수수 깜부기병균에 대한 살균제 활성검정시 실내와 생체검정 결과와의 상관)

  • 김충회;박경석
    • Korean Journal Plant Pathology
    • /
    • v.12 no.3
    • /
    • pp.297-301
    • /
    • 1996
  • 옥수수 깜부기병균에 대한 살균제 활성검정시 실내 검정과 온실 유묘검정과의 상관정도를 조사하기 위하여 5가지 살균제를 공시하여 실내와 온실에서 깜부기병균에 대한 억제효과를 비교하였다. 실내검정방법으로 사용한 최소생육억제농도법, 저지원법, 소생자발아검정법의 결과는 모두 온실유묘검정에서 얻어진 결과와 상관이 높았으며 특히 저지원법은 유묘검정결과와 매우 높은 상관관계를 보였다. 따라서 저지원법과 같은 실내검정방법은 대량의 활성검정시 간이검정방법으로 유용하게 이용될 수 있으리라 생각된다.

  • PDF

Synthesis and fungicidal activity of new ${\beta}$-methoxyacrylate derivatives having thio-enol side chain (티오엔을 곁가지를 가진 메톡시아크릴레이트 화합물의 합성 및 살균활성 연구)

  • Lee, Hyeon-Kyu;Kim, Ji-A;Choi, Eun-Bok;Park, Chwang-Siek;Choi, Gyung-Ja
    • The Korean Journal of Pesticide Science
    • /
    • v.9 no.2
    • /
    • pp.132-139
    • /
    • 2005
  • New ${\beta}$-methoxyacrylate derivatives 1-4 having thio-enol side chain were prepared and subjected to in vivo screening for fungicidal activity against phytopathogenic fungi and many of them showed good fungicidal activities against especially rice blast and wheat leaf rust at 100 ppm.

Selection of Fungicides for the Control of Soybean Black Root Rot Caused by Calonectria ilicicola (콩 검은뿌리썩음병 방제를 위한 살균제 선발)

  • Park, Seong-Woo;Kang, Beom-Kwan;Kim, Hong-Sik;Woo, Sun-Hee;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
    • /
    • v.11 no.1
    • /
    • pp.18-26
    • /
    • 2007
  • Fungicidal screening was performed to control soybean black root rot caused by Calonectria ilicicola through in vitro and greenhouse assays. In in vitro assay, 25 fungicides were assessed by an agar dilution method and a 96-well microtiter plate method. While protective fungicides including dithianon, dichlofluanid, mancozeb, and captan showed a very low activity against the mycelial growth C. ilicicola SC03-15 in the agar dilution method, they displayed potent inhibitory activity against spore germination in a 96-well microtiter plate method with $EC_{50}$ values of 4.65, 0.61, 4.64, and $0.29{\mu}g\;mL^{-1}$, respectively. Ergosterol biosynthesis-inhibiting (EBI) fungicides showed different antifungal activity against mycelial growth and spore germination according to molecules. Difenconazole displayed higher antifungal activity against spore germination rather than mycelial growth, and prochloraz inhibited potently both mycelial growth and spore germination with EC50 values less than $1.8{\mu}g\;ml^{-1}$. In contrast, the other EBI fungicides inhibited more highly mycelial growth than spore germination. Carbendazim+diethofencarb and dazomet also inhibited both mycelial growth and spore germination of C. ilicicola SC03-15 at very low concentrations. In greenhouse assay, carbendazim+diethofencarb effectively controlled a soybean black root rot by drenching 2 days before or after inoculation. In addition, tebuconazole showed potent curative activity against soybean black root rot.

Antifungal activities of sulphamide and dicarboximide fungicides against Botrytis cinerea in several in vitro bioassays (여러 종류의 in vitro 생물검정에서 Botrytis cinerea에 대한 sulphamide계와 dicarboximide계 살균제의 활성 특성)

  • Choi, Gyung-Ja;Kim, Heung-Tae;Kim, Jin-Cheol;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
    • /
    • v.3 no.3
    • /
    • pp.37-44
    • /
    • 1999
  • Two sulphamide (dichlofluanid and tolylfluanid) and three dicarboximide fungicides (iprodione, vinclozolin, procymidone) were used to investigate the correlation between in vitro antifungal activities and in vivo disease controlling activities against Botrytis cinerea, a causal agent of tomato gray mold and to develop efficient in vitro assays. They controlled effectively the development of tomato gray mold disease in vivo and their controlling activities were similar one another. However, several in vitro assays revealed that their in vitro antifungal activities were quite different between sulphamide and dicarboximide fungicides; the formers showed stronger inhibition activities for spore germination than the latters, whereas the formers inhibited mycelial growth less severely than the latters. The results indicate that the fungicides having different modes of action can show different in vitro antifungal activities according to in vitro assays, even if they have similar in vivo disease controlling activities. On the other hand, two rapid and efficient in vitro assays named Microtiter plate methods I (MPM I) and II (MPM II) were developed for the evaluation of fungicides for inhibitory activities against spore germination and mycelial growth of B. cinerea, respectively. The antifungal activities of five fungicides of two chemical groups in MPM I and II were correlated with the inhibitory activities against spore germination and mycelial growth using solid media, respectively.

  • PDF

The Range of Physicochemical Parameters for the Active Ingredients of Fungicides and Insecticides as Crop Protection Agents (작물 보호제로서 살균제와 살충제의 활성 성분에 대한 물리-화학 파라미터의 범위)

  • Song, Sun-Sup;Sung, Nack-Do
    • Applied Biological Chemistry
    • /
    • v.46 no.4
    • /
    • pp.280-284
    • /
    • 2003
  • To develope of diagnosis and estimation system for utility of fungicides and insecticides as crop protection agents, various 10 physicochemical parameters, hydrophobicity (LogP), dipole moment (DM), HOMO energy, LUMO energy, molar refractivity $(MR:\;cm^3/mol)$, polarizability $(Pol:\;A^3)$, van der Waals molecular surface area $(A^2)$, van der Waals molecular volume $(Vol:\;cm^3)$, molecular weight (amu), hydration energy (Kcal/mol) for the active ingredients of 133 fungicides and 152 insecticides were calculated. And then the distribution ranges for each of the physicochemical parameters in fungicides, sterol biosynthesis inhibitors (DMI: demethylation inhibitor), insecticides and acetylcholine esterase inhibitors (AChE) were confirmed. It is suggested that the various compounds based on the range of the physicochemical parametes could be predicted for possibilities as fungicides and insecticides.

Efficient assay for respiration inhibitor using Saccharomyces cerevisiae (Saccharomyces cerevisiae를 이용한 효율적인 호흡저해제 검정법)

  • Choi, Gyung-Ja;Kim, Jin-Cheol;Kim, Heung-Tae;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
    • /
    • v.4 no.3
    • /
    • pp.52-59
    • /
    • 2000
  • A rapid assay to determine respiration inhibition of Saccharomyces cerevisiae by chemicals was developed. S. cerevisiae was harvested with two different liquid media, yeast extract-peptone-dextrose (YPD) medium capable of occurring both glucose fermentation and mitochondrial respiration, and non-fermentable carbon-yeast extract (NFY) medium capable of occurring respiration only Wells in 96-well plate were loaded with each cell suspension and various concentrations of 46 fungicides with various modes of action. n NFY medium, the non-fermentable carbon source, ethanol (NFY-E medium), glycerol (NFY-G medium) or lactate (NFY-L medium), was used. After incubation for $1{\sim}3$ days, minimum inhibitory concentrations (MICs) of the chemicals were recorded in the media. Of the 46 inhibitors employed in this study, four inhibitors of fungal respiration by blockage of electron flux in the mitochondrial respiratory chain, azoxystrobin, kresoxim-methyl, metominostrobin, and trifloxystrobin, exhibited strong antifungal activity in all of NFY media, but no activity in YPD medium. In contrast to this, five N-trihalomethylthio fungicides showed much stronger antifungal activities in YPD medium than three NFY media. Eleven fungicides inhibited growth of S. cerevisiae in all media and the other 26 fungicides showed no antifungal activity in all media. Thus, our rapid and efficient in vitro method can be considered as an alternative assay system for respiration inhibitor.

  • PDF

Evaluation of Toxicity of 23 Pesticides against Harmonia axyridis (Coleoptera: Coccinellidae) Eggs and Adults: Effect on Esterase Activity, Hatchability, and Fecundity (포식성 무당벌레(Harmonia axyridis) 난(卵)의 일부살충제와 살균제에 대한 esterase 활성 및 산란율, 부화율 조사)

  • Cho, Sae-Youll;Park, Young-Man;Park, Yong-Chul
    • The Korean Journal of Pesticide Science
    • /
    • v.11 no.2
    • /
    • pp.117-124
    • /
    • 2007
  • Esterase activity was observed after pesticides treatment in eggs of H. axyridis to select low toxicity pesticide. Egg esterases of H. axyridis were examined using an esterase substrate(${\alpha}$-naphthyl acetate). Three esterase isozymes were detected and the activities were inhibited by organophosphorus insecticide (Chlorpyrifos and Phenthoate), organochlorine insecticide(Methidation), triazole fungicide(Hexaconazole and Triflumizole), and pyrimidine fungicide(Nuarlmol). Fecundity and hatchability in adults and eggs of H. axyridis were examined on selected pesticides. Fecundity and hatchability were significantly reduced from H. axyridis adults and eggs treated with the pesticides and the fungicides showed strong inhibition of esterase isozymes activities. However, we also observed the pesticides and the fungicides showed low or non-inhibition of esterase isozymes activities affected on fecundity and hatchability in adults and eggs.

In Vitro Antifungal Activities of 13 Fungicides against Pepper Anthracnose Fungi (고추 탄저병균에 대한 13종 살균제의 살균 활성)

  • Choi, Yong-Ho;Kim, Heung-Tae;Kim, Jin-Cheol;Jang, Kyoung-Soo;Cho, Kwang-Yun;Choi, Gyung-Ja
    • The Korean Journal of Pesticide Science
    • /
    • v.10 no.1
    • /
    • pp.36-42
    • /
    • 2006
  • For the effective control of pepper anthracnose, in vitro antifungal activities of 13 commercial fungicides were tested on spore germination, mycelial growth, and sporulation of Colletotrichum gloeosporioides, anthracnose fungus. Among them, captan, chlorothalonil, dithianon, fluazinam and folpet completely inhibited the spore germination of C. gloeosporioides at the concentration of 0.8 ${\mu}g/ml$. They were followed by mancozeb and propineb, showing more than 80% inhibition of spore germination at 20 ${\mu}g/ml$. The mycelial growth of C. gloeosporioides was strongly inhibited by fluazinam and nuarimol. Except for nuarimol, most of the fungicides represented more antifungal activity on the spore germination than the mycelial growth of C. gloeosporioides. Azoxystrobin and metominostrobin, Strobilurin fungicides, were only moderately active against the spore germination and the mycelial growth of C. gloeosporioides, but they were effective antisporulant against C. gloeosporioides. From these results, control of pepper anthracnose will have achieved by preventive spray of the commercial fungicides.

lnfluence of Surfactants on Foliar Uptake of Dimethomorph into Cucumber Plant and Fungicidal Activity to Cucumber Downy Mildew (계면활성제가 살균제 Dimethomorph의 오이 엽면 침투성과 오이 노균병 방제 효과에 미치는 영향)

  • Choi, Gyung-Ja;Lim, He-Kyoung;Kim, Jeong-Han;Cho, Kwang-Yun;Yu, Ju-Hyun
    • Applied Biological Chemistry
    • /
    • v.44 no.2
    • /
    • pp.109-115
    • /
    • 2001
  • The foliar uptake of dimethomoiph induced by several nonionic surfactants was measured in order to study the correlations between the uptake rate of dimethomorph and the fungicidal activity to cucumber downy mildew. Dimethomorph was not absorbed in cucumber leaf in the absence of activator surfactant. And the curative effect of dimethomoiph WP to cucumber downy mildew was very low under the concentration of 250 ${\mu}g/ml$. But dimethomorph uptake was remarkably enhanced by addition of nonionic surfactants, such as polyoxyethylene cetyl ether, polyoxyethylene oleyl ether, and polyoxyethylene stearyl ether. And the curative effect to cucumber downy mildew was enhanced with proportion to uptake rate of dimethomorph. The protective effect to cucumber downy mildew, however, tends to decrease with the increase of foliar uptake of dimethomorph. The uptake rate of dimethomorph to cucumber leaf was proportional to the content of polyoxyethylene cetyl ether in formulation, but was decreased with dilution.

  • PDF

Effect of Insecticide and Fungicide on Phytotoxicity of Herbicide in Rice (수도(水稻)에 있어서 제초제(除草劑)의 약해(藥害)에 미치는 살충(殺蟲), 살균제(殺菌劑)의 영향(影響))

  • Chun, J.C.;Hwang, I.T.;Han, M.S.;Jang, B.C.
    • Korean Journal of Weed Science
    • /
    • v.6 no.1
    • /
    • pp.67-75
    • /
    • 1986
  • This study was undertaken to investigate the interrelation between herbicide and insecticide and fungicide on rice (Oryza saliva L.) at various growth stages. Carbamate insecticide BPMC (2-sec-butylphenylmethyl-carbamate) severely inhibited germination of rice when applied alone and in combination with herbicides tested. No germination inhibition was obtained with thio- and dithiocarbamate pesticides. Post-germination growth of rice was severely inhibited by the treatments which were combined with BPMC irrespective of herbicides studied. Phytotoxicity of pendimethalin (3,4-dimethyl-2,6-dinitro-N-1-ethyl propylamine) was reduced by antagonistic effect of organophosphorus compounds. When herbicides were applied with either insecticide or fungicide, post-germination growth of rice was more greatly affected by the concentration of herbicides than that of insecticides or fungicides. Most of herbicide-insecticide or herbicide-fungicide treatments did not cause great phytotoxicity on rice when applied 5 days after transplanting. Foliage activity of phenoxy herbicide 2,4-D (2,4-dichlorophenoxy acetic acid) and MCPA [(4-chloro-o-tolyl) oxy acetic acid] increased with addition of carbamate and urea pesticides.

  • PDF