• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.437-446
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• 2012

As a part of establishing suitability classification for forage production, use of the national soil and climate database was attempted for Italian ryegrass (Lolium multiflorum Lam., IRG) in Gangwon Province. The soil data base were from Heugtoram of the National Academy of Agricultural Science, and the climate data base were from the National Center for Agro-Meteorology, respectively. Soil physical properties including soil texture, drainage, slope available depth and surface rock contents, and soil chemical properties including soil acidity and salinity, organic matter content were selected as soil factors. The crieria and weighting factors of these elements were scored. Climate factors including average daily minimum temperature, average temperature from March to May, the number of days of which average temperature was higher than $5^{\circ}C$ from September to December, the number of days of precipitation and its amount from October to May of the following year were selected, and criteria and weighting factors were scored. The electronic maps were developed with these scores using the national data base of soil and climate. Based on soil scores, the area of Goseong, Sogcho, Gangreung, and Samcheog in east coastal region with gentle slope were classified as the possible and/or the proper area for IRG cultivation in Gangwon Province. The lands with gentle or moderate slope of Cheolwon, Yanggu, Chuncheon, Hweongseong, Pyungchang and Jeongsun in west side slope of Taebaeg mountains were classified as the possible and/or proper area as well. Based on climate score, the east coastal area of Goseong, Sogcho, Yangyang, Gangreung and Samcheog could be classified as the possible or proper area. Most area located on west side of the Taebaeg mountains were classified as not suitable for IRG production. In scattered area in Chuncheon and Weonju, where the scores exceeded 60, the IRG cultivation should be carefully managed for good production. For better application of electronic maps.

• Journal of Animal Science and Technology
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• v.53 no.2
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• pp.127-132
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• 2011

This study was conducted to investigate the effect of feeding colored barley (CB) and whole crop barley (WCB) on performance, egg quality and blood composition of laying hens. A total of 280, 35 weeks old Brown Nick laying hens were allocated into the individual cage for the period of four weeks. Experimental diets contained 2,750 kcal/kg ME and 16% CP, respectively. The treatments consisted of 5, 10, 15% CB and WCB as feed ingredients with control and there were five replications in each treatment. Higher egg production was noticed at 10% level of CB and 5% level of WCB in the diets, respectively. Feed intake was higher in WCB and CB treatments compared to those of the control (P<0.05), but the feed conversion was not different. Yolk color tended to increase both in WCB and CB groups. Haugh unit seemed to increase WCB treatments. When the CB and WCB were fed at 15% level, blood total proteins, albumin, total cholesterol and triglyceride contents were decreased than that of the control birds, but total cholesterol and HDL-cholesterol tended to be higher with the increasing level of WCB. As results, no significant differences were observed in performance and egg quality with different levels of dietary WCB and CB. Thus, CB and WCB can be substituted as a feed ingredient up to 15% level in the laying hens' diet. However, further studies are required by feeding more than 15% levels of CB and WCB in the diet of laying hens.

• Journal of Animal Science and Technology
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• v.53 no.2
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• pp.147-154
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• 2011

This study was conducted to evaluate the effects of saponin contained plant extracts on in vitro rumen fermentation characteristics and methane production. Ruminal fluid was collected from rumen cannulated Hanwoo steers fed rice straw and concentrate (5:5). Collected rumen fluids, corn starch and buffer including saponin contained plant extracts (ginseng, Ogapi, soapwort, tea plant and yucca; 0.5%/15 ml) were incubated at $39^{\circ}C$ for 24 h. All incubations were repeated five times. Rumen pH in all treatments was lower (p<0.05) compared with that of the control (no addition) during incubation time. The concentration of total VFA in all treatments was higher (p<0.05) than that of the control after 12h incubation. Compared with the control, the concentration of acetate and propionate in all treatments was lower and higher after 6h incubation, respectively. The concentration of $NH_3$-N in all treatments was lower (p<0.05) than that of the control except for Ogapi or yucca extracts supplementation. The number of protozoa in all treatments was significantly (p<0.05) lower than that of the control except for soapwort extract supplementation. The total gas production and methane production in all treatments was higher (p<0.05) and lower (p<0.05) compared with the control, except for ogapi or soapwort extracts supplementation after 12h incubation, respectively. Therefore, reduction in methane production by saponins may could be results from decreased protozoal population without any negative in vitro fermentation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.211-220
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• 1999

The corn is mostly planted in mid-April in single-cropping silage system if possible, however, in a double-cropping silage system, the corn planting date is delayed until after the mid-May. The objective of this study was to evaluate agronomic characteristics and forage performance of the eight government recommended corn hybrids for silage at two dates of planting. Split-plot design replicated three times was used, that is, main plots were planting dates, sub-plots were eight hybrids at Suweon in 1997. Days from planting to silking of optimum planting was 84 days and that of late was 69 days. The difference in silking day among the hybrids was 6 days at optimum planting and 2 days at late planting. Plant height of optimum planting corn was higher than that of late one, however, ear height and stem diameter of optimum planting were lower than those of late planting corn. Among the corn hybrids tested, plant heights of 'P3156' and 'P3163' were higher than those of the other hybrids. Ear height of 'DK713' was the lowest among the corn hybrids and stem diameter of 'P3394' was thicker than that of the other hybrids. European corn borer(ECB) damage(16.6%) at optimum planting was lower than that(49.9%) of late, however, there were no significant ECB damage differences among the corn hybrids tested. Whole plant dry matter(DM) contents of 'P3525', 'P3394' and 'P3352' hybrids were higher than those of the other corn hybrids at harvest. Ear percentages of the total dry matter(DM) of 'P3394' and 'P3156' were higher than those of the other corn hybrids. Dry matter(DM) and total digestible nutrients(TDN) yields(19,696 and 14,621kg/ha) at optimum planting were higher than those (17,163 and 12,570kg/ha) of late planting, while there were no significant differences in DM and TDN yields among the corn hybrids tested. And those hybrids with greater proportion of grain in the whole plant have higher DM and TDN yields. Correlation coefficients for days from planting to silking with DM and TDN yields were $0.84^{**}$ and $0.87^{**}$, and those for ear percent were $0.86^{**}$ and $0.87^{**}$. Results of this study indicate that optimum planting date(15 April) is better than late planting date(16 May) in agronomic characteristics and forage production of silage corn. Days from planting to silking and ear percent as welI as other characters are important factors screening the corn hybrid for silage.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.247-254
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• 2011

The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.645-654
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• 2014

This study was conducted to investigate the effect of 1-methylcyclopropene (1-MCP, $1.0{\mu}L{\cdot}L^{-1}$), a known ethylene action inhibitor, on fruit quality and incidence of physiological disorders during a simulated marketing period at $25^{\circ}C$ for 20 days in early-season Asian pear (Pyrus pyrifolia Nakai) 'Hanareum' that had been treated with 0, 0.5, 1.2 or 2.4% $GA_{4+7}$. Weight loss of stored fruits increased with $GA_{4+7}$ concentration, and the 1-MCP treatment slightly reduced the weight loss rates during the marketing period. Flesh firmness decreased abruptly in all 1-MCP-untreated fruits as the storage period extended to 10 d, whereas the firmness of 1-MCP-treated fruits remained high (> 30 N) during 15 days shelf-life. The effect of 1-MCP was significantly reduced when fruits were subjected to increased GA concentration. Higher soluble solids content and acidity during extended shelf-life were also apparent in 1-MCP-treated 'Hanareum' pears. The L-values (lightness) and hue angles of 1-MCP treated samples were higher than those of controls during 20 days shelf-life, but the a-value (redness) was lower in 1-MCP treated fruits. 1-MCP treatment did not decrease the level of ethylene evolution regardless of $GA_{4+7}$ concentration during shelf-life in early-season Asian pear 'Hanareum'. By contrast, 1-MCP treatment decreased the respiration rate significantly during shelf-life. The efficacy of 1-MCP was greatest in the GA-untreated fruit and was reduced as the $GA_{4+7}$ concentration increased. 1-MCP treatment influenced the severity of physiological disorders including core browning and mealiness: 1-MCP treatment completely blocked the incidence of core browning of during 15 days shelf-life, and reduced the severity of mealiness during 20 days shelf-life regardless of $GA_{4+7}$ concentration. Based on our results, we conclude that the use of $1{\mu}L{\cdot}L^{-1}$ 1-MCP can be of great benefit for maintaining quality and preventing physiological disorders in early-season pear cultivar 'Hanareum' pear, whereas its efficacy decreases with the concentration of $GA_{4+7}$ whereas its efficacy gradually decreases when the concentration of $GA_{4+7}$ paste increased.

• The Korean Journal of Food And Nutrition
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• v.23 no.3
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• pp.352-360
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• 2010

This study was performed to develop a Korean natural cheese with traditional medical wine, making it different from foreign natural cheese. The effects of cheese with Sasam(Codonopsis lanceolate) wine(CLW) on the quality properties during the ripening period of natural cheese were investigated. The properties investigated were growth of lactic acid bacteria, characteristics of ripening, and sensory characteristics. Four vats of cheese were made on the same day from the same tank of fresh milk. Cheese samples were prepared with CLW at 2.0%, 4.0% and 6.0% of raw milk. Changes in gross composition, viable cell counts, pH, water soluble nitrogen(WSN), non casein nitrogen(NCN), non protein nitrogen(NPN), and proteolysis during maturation were measured. Polyacrylamide gel electrophoresis(PAGE) patterns were determined with control cheese. Viable cell counts of control and CLW cheese were not significantly different. The pH of CLW cheese increased gradually during maturation, and saponin levels and levels of NPN, NCN, and WSN were higher in CLW cheeses than control cheese. For most compositional data, the 4.0% CLW cheese was most similar to the control cheese. The PAGE pattern of cheese caseins indicated that the CLW cheeses degraded more rapidly than the control cheese. Control and 2.0% CLW cheese had good sensory scores, while scores for 4.0% and 6.0% CLW cheese were lower. However, sensory data depreciated with added levels of CLW, especially at a level of 4.0% or more. Further studies on levels of CLW and processing methods are required to improve sensory quality.

• Food Science of Animal Resources
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• v.20 no.2
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• pp.159-165
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• 2000

Glycomacropeptide(GMP) was purified from cheese whey which is obtaining as a byproduct in cheese producing. Cheese whey was first concentrated 10 times with a ultrafiltration aparratus, and then heated at 95$^{\circ}C$ for 5 min. The concentrated fraction was centrifuged at 20,000$\times$g for 30 min to remove fat layer. The supernatant layer enriched GMP protein was fractionated by ion exchange chromatography on DEAE-Sepharose Fast Flow column. GMP was bound to DEAE resin and eluted with 0.1~0.25 M NaCl when using a linear NaCl gradient from 0 M to 0.5 M. The purified GMP gave a single band of 24 kDa which seems to be trimer molecular weight in SDS-PAGE, and migrated to the same molecular weight with control GMP obtained commercially. Its amino acid composition were consistent with that of standard GMP. About 0.71 g of GMP was recovered from 1 L of cheese whey. These results indicate that glycomacropeptide could be simply purified from cheese whey by using ultrafiltration and DEAE column chromatography.

• Journal of Aquaculture
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• v.11 no.4
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• pp.573-580
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• 1998

Red spotted grouper, Epinephelus akaara is distributed in the south and west coasts of Korea. The natural stocks of the fish are decreasing sharply year by uear because of reckless overfishing. This research was carried out to understand general informations on maturation, sex composition and sex-reversals of the fish. Annual fishing uields of red spotted grouper in the castal area of Byonsan Peninsular of Kora decreased over 10% from 1992 to 1994. The main fishing season was from May to July with fishing gear of Hand-lines. Gonadosomatic index (GSI) and condition factor were highest on early and late July, respectively, thus main spawning reriod was assumed from late July to early August. The relationship between total length (X) and body weight (Y) for wild adults was represented as a regression, Y=$0.0169X^{2.9705}$, ($r^2$=0.96). Frequency of sex of wild red spotted gouper showed that the number of female below 38cm in total length was more than that of male, and hermaphrodite mainly occurred from 28cm to 32cm in total length the frequency of male and female were almost same. Also hermaphrodite occurred mainly between 25~29cm. Sex reversal ration of the adults reared in a tank for a year with different sexual compositions revealted that the frequency of female reversed from male was more than that of male reversed from female at 1:1 and 1:2 stocking densities of female and male, respectively. Also, about 20% of female was reversed to male when all females were reared. And the size of the fish reversed to male was larger than that of non-reversed female.

• Journal of Aquaculture
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• v.20 no.1
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• pp.19-25
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• 2007

For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.