• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.226-230
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• 2007

In order to achieve high-level expression of interferon-${\alpha}1$ (IFN-${\alpha}1$) during fed-batch fermentation of recombinant E. coli, effects of oxygen supply and induction temperature on the expression of recombinant proteins were evaluated. Supplementation of oxygen and its transfer into cells is one of the most important parameters involved in the design and operation of mixing-sparging equipment for bioreactors. Generally, higher oxygen supply stimulates cell growth of aerobic microorganism and consequently the amount of products is increased. In this study, the optimum aeration strategy for the higher production of IFN-${\alpha}1$ during fed-batch fermentation of recombinant E. coli was surveyed. The growth of the cells was also monitored with four different concentrations of dissolved oxygen (DO; limiting, 20%, 35%, 50%) conditions. The DO was controlled by varying aeration rates of air and pure oxygen. Oxygen uptake rate (OUR) and specific oxygen uptake rate (SOUR) were evaluated and compared for the enhanced growth and induction of the cells and IFN-${\alpha}1$, respectively. We confirmed that increased DO by additional oxygen supply, up to 35%, can improve the production of IFN-${\alpha}1$ during the fermentation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.69-73
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• 1976

Mortality of clam, Meretrix lusoria was measured during the experimental cultures in different sediments compositions and in different salinities of sea water. Experimental groups maintained in silt and clay showed significant mortality, while those treated in sand and in sand-silt showed little mortality. In the groups cultured in silt and clay, young groups of approximately 2.5cm in shell length were subjected to early and high mortality (approximately eighty-five per cent in foully-one days). The larger the shell size was, the later and the lower the mortality occurred. In different salinity, the groups maintained in silt and clay showed different mortalities. It is observed that the survival rate of the shell in the sea water of low salinity was higher than that in the high salinity water. Oxygen consumption of isolated gill tissue showed a little difference between the groups maintained in silt and clay and those in sand (the control-group). Consequently, it may be stated that the mortality results mainly from the deposition of silt and clay, although the survival rate of Meretrix lusoria depends also on water temperature, salinity, dissolved oxygen, body size ana other factors.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.138-144
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• 2008

The effects of salinity on oxygen consumption, operculum movement number and ammonia nitrogen excretion were examined in cultured tilapia, Oreochromis mossambicus (total length 10$\pm$1.0 cm, total weight 17.3$\pm$4.2 g). The fish were exposured to certain salinities (0, 3, 9, 15, 21, 27 and 32 psu) step by step with 3 psu$\cdot$d$^{-1}$ and changed rapidly from certain salinity to another salinity. The oxygen consumption of the fish which was transferred step by step showed increasing tendency in the range of 3 $\sim$ 27 psu, but markedly appeared very low value at 32 psu. The tolerance limit in tilapia by salinity change without acclimation was shown 23.3 psu from 96-h TL$_{50}$. The oxygen consumption, operculum movement and ammonia nitrogen excretion of fish which was transferred rapidly from certain salinity to each salinity (0, 9, 15, 21 and 32 psu) showed a changing point at 15 psu; they showed increasing and/or decreasing pattern before 15 psu, and showed decreasing pattern after 15 psu. From these results, it was concluded that the appropriate salinity without physiological change for Java tilapia was below 15 psu.

• Journal of Life Science
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• v.13 no.1
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• pp.21-28
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• 2003

The purpose of this experiment was to compare the energy expenditure and the physiological response among two groups by percent body fat(group A: 30-35% body fat, B: 35-40% body fat) to walking and running at several equivalent speeds. Subjects in group A and B followed A group(mean$\pm$SD, age; 24.0$\pm$0.4yrs, body fat; 32.3$\pm$0.7) and B group (age; 25.2$\pm$0.7yrs, body fat; 36.7$\pm$0.9). The walking and running protocol consisted of treadmill speeds for five min at each of the following speeds: 5.0, 5.5, 6.0, 6.5, 7.0 km.$hr^{-1}$. The obtained data reveal in group A, the rate of oxygen consumption and energy expenditure was higher during walking compared to running ate treadmill speeds $\geq$ 6.6km.$hr^{-1}$. In group 5, the rate of oxygen consumption and energy expenditure was higher during walking compared to running ate treadmill speeds $\geq$ 6.8km.$hr^{-1}$. Heart rates and respiratory exchange ratio were higher at treadmill speeds $\geq$5.8 in group A and $\geq$5.5 in group B. these findings demonstrated that a difference of percent body fat in obese women have no large effect on energy efficiency of walking, but walking within speeds 6.5~7.0km/hr resulted in rates of energy expenditure that were as high or higher than jogging at the same speeds even though the relative stress was greater during walking.

• Ocean and Polar Research
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• v.36 no.1
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• pp.39-47
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• 2014

The black scraper Thamnaconus modestus was a commercially important fish species in the 1980s, but suddenly its commercial significance decreased in the 1990s mainly due to continuous overfishing. Recently, in order to reverse the depleted stocks of the black scraper and help the species recover, seed production technology has emerged. This has led to the farming of the black scraper in several parts of the southern coast of Korea. Since detailed research on its metabolism in relation to water temperature has been scanty, this was the investigative focus of the present study. The standard metabolism rates of the black scraper (9-10 months old, total length=$22.6{\pm}0.8cm$, wet weight=$140.3{\pm}13.9g$) were measured at seven different water temperature settings (12, 15, 17, 20, 23, 26, $28^{\circ}C$) to understand the relationship between metabolism and water temperature. Relationships between water temperature (WT) and oxygen consumption rate (OCR) were obtained as SOCR (weight-specific oxygen consumption rate) = 0.0117WT - 0.0135 ($r^2=0.9351$) and IOCR (oxygen consumption rate per individual) = 1.8160WT - 5.4007 ($r^2$ = 9428). The $Q_{10}$ (temperature sensitivity), an indicator of the sensitivity of biological function to temperature, was analyzed. In our experiment, when the water temperature increased, the $Q_{10}$ value decreased. The $Q_{10}$ value was 6.27 in waters where the temperature ranged from $12-15^{\circ}C$ and this was much higher than the values obtained in waters where temperatures ranged between (1) $15-23^{\circ}C$ and (2) $23-28^{\circ}C$. Consequently, it was shown that the black scraper is a warm water species and inhabiting waters in the temperature range from $15-28^{\circ}C$ is deemed appropriate.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.41-41
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• 1992

새로이 합성된 1,4-Naphthoquinone(1,4-NQ) 화합물의 세포독성의 확인과 그 작용기전을 규명코져 본 실험을 수행하였다. 분리된 흰쥐 간세포를 1,4-NQ유도체들과 반응 시켰을때 이들 중 YC 001과 YC 008은 독성을 나타냈으나 YC 012는 독성율 나타내지 아니하였다. 세포독성은 세포치사, lactate dehydrogenase 유출 및 산소소비의 억제등으로 확인하였다. 세포 독성의 기전은 mitochondria에서의 전자흐름 차단작용과 microsome에서 redox-cycling 기전에 의한 산소리디칼 생성의 두가지에 대하여 검토하였다. 분리된 mitochondria를 YC 001과 YC 008은 반응 시켰을때 mitochondria의 호흡은 완전히 억제되었다. 그러나 YC 012는 전혀 작용을 나타내지 아니하였다. 이는 간세포에 대한 독성작용과 일치하는 결과이며 따라서 mitochondria에 대한 억제 작용은 이들의 세포독성에 주요한 요인이라고 추측되었다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.293-296
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• 1979

The effects of temperature and salinity on tile rates of ammonia and amino nitrogen excretion, and oxygen consumption were measured for Crassostrea gigas. There was variability with temperature and salinity changes in both the rates of nitrogen excretion and the proportionality between ,ammonia and amino acids in the excreta, and also in the rates of oxygen consumption. Rates of nitrogen excretion and oxygen consumption were markedly decreased with increase in salinity, especially at high salinity-high temperature, whereas at low salinity-high temperature condition they were significantly increased. These changes are considered as the responses of physiological tolerances to the high temperature stress and the results of the metabolic temperature compensation at the low salinity-high temperature condition. Most of nitrogenous excretory products was ammonia, and large amounts of amino-nitrogen was excreted, and especially the rate of amino-nitrogen excretion was dominant at $32.5\%_{\circ}-22^{\circ}C$. The amounts of amino-nitrogen excreted by animals were decreased in the medium of high salinity and increased in the medium of low salinity through the experimental temperature. The atomic ratios of oxygen consumed to ammonia-nitrogen excreted (O: N ratio) was low at the low temperature $(15^{\circ}C)$, and was high at $22^{\circ}$ and $29^{\circ}C$ in the medium of 32.5 and $37.5\%_{\circ}$ but low in the low salinity $27.5\%_{\circ}$.

• Journal of the Korea Safety Management & Science
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• v.12 no.3
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• pp.135-143
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• 2010

본 연구에서는 대기오염물질인 유해 황산화물 가스를 이산화티탄 촉매 반응기와 연면 방전 반응기를 조합한 반응기에서 플라즈마 방전반응에 의하여 주파수 변화, 체류시간, 전극의 굵기, 첨가 모의가스 등의 공정 변수를 변화 시켜 분해제거 실험을 하였다. 실험 결과 황산화물의 분해제거 실험에서 주파수 10kHz에서 소비전력 19W에서 분해제거율은 99%이었으며 이산화티탄 촉매반응기를 부착한 경우가 없는 경우보다 5%이상 증가효과가 이었다. 첨가가스로 메탄을 첨가한 경우 분해제거율이 증가하였고, 산소농도가 높아질수록 증가하였다 또한 이산화 탄소를 첨가한 경우 분해율은 감소하였다.

• Journal of Korean Society of Water and Wastewater
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• v.24 no.4
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• pp.387-394
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• 2010

The influent COD of municipal wastewater has been divided into 4 fractions; readily soluble biodegradable, slowly particulate biodegradable, soluble and particulate unbiodegradable COD. The mathematical modeling of biological wastewater treatment processes and the design and operation of nutrient removal plants require a reliable and accurate estimate of the composition of influent wastewater COD. COD utilization rate is proportional to the oxygen uptake rate(OUR), so a batch biodegradation test with OUR measurement has been effectively used for the determination of COD fractionation. But the mathematical model of COD utilization and heterotrophs synthesis is essential to interpret the OUR measurement. Mamais method is another method for determining readily biodegradable soluble COD. Like the OUR test method, batch biodegradation test is necessary but it does not require mathematical model. These two methods for determining COD fractionation are introduced here in detail. Experimental results showed that COD composition by Mamais method is not different to that by OUR test method so, either of them can be used.

• Korean Journal of Environmental Biology
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• v.31 no.3
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• pp.204-212
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• 2013

This study analyzed the occurrence of tunic softness, survival rate, metabolic rate and histopathologic changes arising from the effect of hypoxic environment in order to find the causes of occurrences of tunic softness, which manifests as the key phenomenon of mass mortality of Halocynthia roretzi. Regarding the survival of H. roretzi with reduction in dissolved oxygen, all the entities died on the 4th day of exposure to the dissolved oxygen concentration of $2mg\;L^{-1}$ while 50% mortality was observed on the 5th day of exposure to the dissolved oxygen concentration of $3mg\;L^{-1}$. Therefore the 5 days-$LC_{50}$ was found to be $3.55mg\;L^{-1}$ (1.86~$4.96mg\;L^{-1}$). However, occurrence of tunic softness was not observed during the period of exposure to low oxygen concentration. The oxygen consumption rate significantly decreases at the dissolved oxygen concentration of less than $5mg\;L^{-1}$ in comparison to the control group. Therefore, it is presumed that H. roretzi controls the respiration rate for prescribed period of time when exposed to hypoxic environment. Regarding the histopathologic changes in the gill, digestive gland and cyst of H. roretzi due to hypoxic environment, necrosis of epithelial layer, in filtration of blood cells, and condensation of nucleus that compose each of the organs were observed. Regarding morphological changes, the decrease in volume with shrinking of the tunic, discoloration of the internal organs and necrosis of gill and hepatopancreas were observed.