• The Korean Journal of Ecology
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• v.27 no.6 s.122
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• pp.347-353
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• 2004

This study was carried out to analyze pH, Electrical conductivity(EC), cation and anion on rainfall and throughfall raining at industrial area(Onsan and Nongso of Ulsan city), suburban area(Myeongsek-myeon of Jinju city) and rural area(Haman and Changyoung-gun), and to investigate $SO_2\;and\;NO_2$ concentration in the air of industrial area and rural area, and to survey degree of decline of Pinus thunbergii growing at each area. The rainfall pH was higher than the throughfall pH at the all surveyed areas. The rainfall pH and throughfall pH was $4.76{\sim}5.15\;and\;4.64{\sim}4.98,\;4.95{\sim}5.30\;and\;4.87{\sim}5.13,\;5.29{\sim}5.76\;and\;5.12{\sim}5.73$ at the industrial area, suburban area and rural area, respectively. The EC of rainfall and throughfall was $13.64{\sim}17.22\;and\;41.60{\sim}103.59,\;4.18{\sim}8.65\;and\;17.02{\sim}23.89,\;4.09{\sim}6.65\;and\;13.75{\sim}21.44$ at the industrial area, suburban area and rural area, respectively. Generally EC at the industrial area was higher than those of suburban area and rural area. In the correlation analysis among Degree of decline on Pinus thunbergii and ingredients of rainfall, it showed that there was a negative correlation between Degree of decline and pH. There was a positive correlation between pH and $Cl^-$ respectively.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.81-87
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• 2003

The digestive system of the abalone, Haliotis discus hannai consists of radula sac, esophagus, crop, stomach, intestine (anterior, mid and posterior intestine) and hepatopancreas. The epithelial layer was composed of ciliated columnar cells, mucous cells and granular cells. And epithelium thickness of the crop was thicker (90.80 ${\mu}$m) than those of other regions. Mucous cells of PAS positive in the esophagus were more advanced than those of other regions. The contents of mucous cell were neutral and acid mucosubstance in the esophagus and the anterior, mid and posterior intestine. And it seem to be lipid in the crop and stomach.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.177-190
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• 2011

This study was conducted to determine effects of dietary herbaceous peat on in vitro fermentation and milk production in dairy cows. Ruminal pH, gas production, VFA (volatile fatty acid), Ammonia-N, and rumen degradability were examined by the addition of three times over 0, 1, and 5% herbaceous peat with substrate of timothy hay, and the change of rumen fermentation characteristics were evaluated. In 0, 3, 12 and 24 hours cultivation, all treatments did not show a significant difference but the control at 6 hours appeared significantly lower pH compared to 1 and 5% treatments (p<0.05). The gas production of the treatments significantly increased until 12 hours of cultivation compared to control (p<0.05), the rumen ammonia concentration showed a tendency to increase until 24 hours in all treatment groups, and there was no significant difference between treatments. About the rumen degradability, 5% treatment showed higher rumen degradability in all hours than control and 1% treatment (p<0.05). Meanwhile, for in vivo trial, 16 heads of Holstein lactation dairy cows were selected for experiment for four weeks in order to research the change of milk yield, milk compositions and change of somatic cell counts of lactation dairy cows by herbaceous peat feeding. The milk yield of vitamin C and herbaceous peat treatments (T3) was 25.0 kg but the control was 23.2 kg, herbaceous peat treatment (T1) was 23.1 kg, and vitamin C treatment (T2) was 23.4 kg, so there was linear increase effect of milk yield by T3. The partial significance of the milk (fat, milk protein, lactose, MUN and SNF) and change of somatic cell count before and after experiment by the control and treatments about change of milk and somatic cell counts (p<0.05) were recognized. About change of milk in the first half (1~2 weeks) and latter half (3~4 weeks) during four weeks of experiments period, the herbaceous peat supplement treatments showed a tendency of significant decrease of quality of milk protein and SNF. The control and treatments did not show significant change of blood nutrients (total protein, cholesterol, NEFA, BUN), liver function component (AST, GGT) and minerals (Ca, P, Mg) before and after experiment. In summary, it is judged that herbaceous peat feeding for lactation dairy cows would be recommendable based on the results of milk, somatic cell count physiologically.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.72-81
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• 2012

We investigated the effects of acid mine drainage (AMD) from abandoned coal mines on benthic macroinvertebrate communities in the upper reaches of the Nakdong River from May to October in 2009. Qualitative and quantitative sampling (Surber sampler: $50{\times}50$ cm; mesh size, 0.2 mm) was conducted at 7 study sites and 3 control sites in the study area. We thus sampled 117 species belonging to 53 families, 15 orders, 6 classes, and 5 phyla; the Ephemeroptera, Plecoptera, and Trichoptera group (EPT-group) represented the majority of the benthic macroinvertebrate community (71 species; 64.5%). In the quantitative sampling, a total of 11,575 individuals belonging to 58 species of benthic maroinvertebrates were sampled from the study sites (Sites 1-7), whereas 2,844 individuals belonging to 79 species were sampled from the control sites (Sites A-C). Tolerant species such as oligochaetes, $Epeorus$ $pellucidus$, $Baetis$ $fuscatus$, Hydropsychidae species, and Chironomidae species were predominant in the study sites. The community indices for the study sites, such as MacNaughton's dominance index (DI) (mean${\pm}$SD, $0.52{\pm}0.21$; range, 0.33-0.85) and the Shannon diversity index ($H^{\prime}$) ($2.06{\pm}0.60$; 1.06-2.57), were different from those for the control sites (DI: $0.29{\pm}0.07$, 0.22-0.35; $H^{\prime}$: $3.13{\pm}0.14$; 3.03-3.30). In the study sites, shredders and scrapers were scarce, whereas gathering-collectors (mainly Chironomidae species) were relatively abundant, as were clingers and burrowers. The detrended correspondence analysis (DCA) and similarity analyses showed that benthic macroinvertebrate communities in the study sites were clearly separated from those of the control sites, with the greatest dissimilarity being noted at the uppermost study site (Site 1), which is located close to an abandoned coal mine. The Korean saprobic index (KSI) and the ecological score using benthic macroinvertebrates (ESB) showed that the uppermost study site (Site 1) was ${\alpha}$-mesosaprobic or heavily polluted, whereas other study sites were in a fair or relatively good condition.

• Journal of Korean Society of Forest Science
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• v.85 no.1
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• pp.84-95
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• 1996

Environmental pollution has recently been progressed in the metropolitan and industrial areas of Korea and concerns have been evolved against the chronic effects of the pollution on natural ecosystem. This study was carried out to investigate the environmental pollution impacts on ion input into forest ecosystems and soil environmental changes. Study plots were established at Seoul, Ulsan, Yeochon, and Seosan for pollution sites and at Pyungchang for a non-pollution site. Atmospheric deposition was measured with rain, throughfall, and stem flow samples collected in the forest areas. Soil chemical properties were investigated to compare the pollution impacts on the sites. Precipitation acidity in the metropolitan and industrial areas ranged from pH 4.5 to 5.5, showing the levels lower than pH 5.8 of mountain area. Ion concentrations in the precipitation had increased significantly while passing the crown layer in the metropolitan and industrial areas, showing the increase by 4 times at the maximum. Total ion input in the metropolitan and industrial areas was greater than that in mountain area by approximately 2-3 times. Soil acidification caused by acidic ion input seemed to be greatest at Seoul, showing pH 1 decrease compared to that of Pyungchang. Soil canon contents were relatively high in the metropolitan and industrial areas. Although the canon leaching loss was not apparent, soil acidification process seemed to be continued by acidic ion input. Environmental pollution in the metropolitan and industrial areas exerted changes in ion input into the forest ecosystems and soil conditions. The chronic effects of environmental pollution should be monitored and investigated further to explain the processes of ecosystem change and the impacts on plant growth.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.4
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• pp.304-312
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• 2013

This study was conducted to investigate the effect of total neutral detergent fiber (NDF) and forage-derived NDF (fNDF) on nutrient intake and milk production in Holstein dairy cows. Thirty-six Holstein dairy cows at weeks 4 to 20 of lactation were included in this study. The cows were arranged in a randomized complete block design and assigned to one of six diets: 1) 38% NDF with 18% fNDF (HN-HF); 2) 38% NDF with 15% fNDF (HN-MF); 3) 38% with 12% fNDF (HN-LF); 4) 34% NDF with 18% fNDF (LN-HF); 5) 34% NDF with 15% fNDF (LN-MF); and 6) 34% with 12% fNDF (LN-LF). The cows were fed a total mixed ration twice daily (07:00 and 18:00 h) and given ad libitum access to feed and water throughout the experiment. Data on the intake of nutrients (DM, NEl, CP, NDF, fNDF, NFC), milk yield, composition, fat-corrected milk (FCM), fat and protein corrected milk (FPCM), and energy corrected milk (ECM) were analyzed. Lower dietary concentrations of total NDF or fNDF resulted in an increase in DM, NEl, and NFC intake (p<0.01), milk yield (p<0.05) and a lower milk fat concentration (p<0.01). The LN treatment showed no significant differences in milk fat concentration compared to the HN however, further reduction in fNDF decreased milk fat content (p<0.01). The lowest level of milk fat was observed in the LN-LF diet group. Changes in the dietary concentrations of NDF or fNDF did not affect the concentrations of milk protein or SNF (solid not fat), although the yield of milk protein and SNF increased with higher milk yield (p<0.01). The level of fNDF influenced the level of FCM (p=0.07), as well as FPCM and ECM yield (p<0.05). A highly positive correlation between fNDF intake and milk fat concentration was observed in animals with low NDF compared to those offered high NDF. A strong correlation was also observed between milk yield and low NDF intake compared to high NDF intake.

• Journal of Life Science
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• v.20 no.4
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• pp.487-495
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• 2010

A microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as Psychrobacter aquimaris by analysis of 16S rDNA sequences, and named P. aquimari LBH-10. This strain produced an acidic carboxymethylcellulase (CMCase), which hydrolyzed carboxymethylcellulose (CMC), cellobiose, curdlan, filter paper, p-nitrophenyl-$\beta$-D-glucopyranoside (pNPG), pullulan, and xylan, but there was no detectable activity on avicel and cellulose. The optimal temperature for CMCase produced by P. aquimari LBH-10 was $50^{\circ}C$ and more than 90% of its original activity was maintained at broad temperatures ranging from 20 to $50^{\circ}C$ after 24 hr. The optimal pH of the CMCase was 3.5, and more than 70% of its original activity was maintained under acidic conditions between pH 2.5 and 7.0 at $50^{\circ}C$ after 24 hr. The optimal pH of CMCase produced by P. aquimaris LBH-10 seems to be lower than those produced by any other bacterial and fungal strain. $CoCl_2$, EDTA, and $PbCl_2$ at a concentration of 0.1 M enhanced CMCase-produced P. aquimaris LBH-10, whereas $HgCl_2$, KCl, $MnCl_2$, $NiCl_2$, and $SrCl_2$ inhibited it.

• Journal of the Korean GEO-environmental Society
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• v.5 no.4
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• pp.13-24
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• 2004

This study was carried out to plan the prevention of the generation and discharge of Acid Rock Drainage (ARD). The Acid Base Accounting(ABA) test was performed for geological materials such as pit wall, waste rock and stream sediments near the Imgi abandoned pyrophyllite mine in Busan, Korea. In addition, hydraulic characteristics were tested with the disk tension infiltrometer around the waste rock dump. Maximum Potential Acidity(MPA) of geological materials near the Imgi mine was 246.942kg $H_2SO_4/t$, and maximum Acid Neutralising Capacity(ANC) was 8.7kg $H_2SO_4/t$. These results indicate the pit wall and waste rock, except most of stream sediments are acid generating geological materials. These have salt and free hydrogen ion which resulted from oxidation of sulfides. Hence they could be convert rain water to acid rock drainage. Although the waste rock dump of the Imgi mine have very low infiltration rate, slopes of the waste rock dump have many "V" type erosion gullies and multi-layers. These gullies and multi-layers have coarse clastic particle layers which have very large hydraulic conductivity. Through this coarse clastic particle layer a large part of rain flow into ground. And also this layer could function as aeration path which induced oxidation of sulfide minerals and generation of ARD continuously.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.237-245
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• 1991

Artificial acid rain (pH 3.0, 4.0 and 5.0) was treated on thd seeds of 5 coniferous species to examine its effects on germination and seedling growth. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water(pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots three times per week. About 5mm of artificial acid rain was treated each time from early April to early October, 1990. Germination, seedling establishment and seedling growth were measured and compared among the treatments. The results were summarized as follows ; 1. Germination, seedling establishment rate of Larix leptolepis and Picea koraiensis were highest on the pH 5.0 plot whereeas those of Abies holophylla and Cryptomeria japonica were highest on the control plot, and those of Abies koreana showed the highest value on the pH 3.0 plot. 2. The differences in seedling height growth of Larix leptolepis was signif- icant at 1% level among the treatment levels of acid rain, whereas those of other species were not significant, Height of Larix leptolepis, Abies koreana and Picea koraiensis were highest on the pH 4.0 plot, and that of Abies holuphylla and Cryptomeria japonica were highest on the pH 5.0 plot. 3. Seedling dry weight of Larix leptolepis and Abies holophylla differed significantly at 1% level among the treatments, but those of other species did not differ. Highest seedling dry weight of Larix leptolepis and Abies koreana were observed on the pH 4.0 plot, and that of Abies holophylla and Cryptomeria japonica did on the pH 3.0 plot, and that of Picea koraiensis did on the control plot. 4. With decrease of pH value of artificial acid rain, the number of injured needle and injured individual tended to be increased, obviously. 5. The differences of soil acidity were highly significant among the treatments for all species.

• Journal of Conservation Science
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• v.25 no.4
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• pp.451-464
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• 2009

To do conservation for ceramics, sometimes it needs to clean the ceramics chemically to remove stains on the ceramics, which should be performed with caution. This study will provide quantitative data about the extraction characteristics of major elements from ceramics through the soaking test to evaluate the stability of ceramic cleaning agents. As soft textured pottery(fired at $700^{\circ}C$) had high absorption, it showed higher extraction characteristics in short soaking time. Especially the strong acid among chemical cleaning agents caused the expoliation and the color change on the surface of soft textured pottery. But these color change had no effect on the physical characteristics of soft textured pottery. The hard textured pottery(fired at $1,000^{\circ}C$) is relatively stable in the cleaning agent because it is ascertained the extraction of that was not enough and also it had little chromatic and physical change by strong acid. Porcelains(Celadon, Whiteware and Puncheong ware) was safer than the soft textured pottery due to their low extraction. But it should take precautions to clean the porcelains with lots of cracklewares such like Puncheong ware chemically in connection with the extraction characteristics and their cracklewares.