• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.289-293
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• 2010

3-MCPD created in manufacture process was regulated in our country about soy sauce and HVP. The latest paper reported that Bound 3-MCPD is created as intermediate. Germany common risk assesment reported that Bound 3-MCPD must be reduced because Bound 3-MCPD can be created in estimation circle when this is hydrolyzed in human body, but the data about the toxity of Bound 3-MCPD is lack. Therefore, We analysis about 209 items food such as soy sauce, seasoning food and meat-eating manufactured goods using bound 3-MCPD analysis method developed recently. As result of survey, bound 3-MCPD detected in 8 items among 44 traditional sauce (0.02~0.28ppm), 8 of soup 12 items (0.01~0.96ppm), in 22 items of sauce 60 items (0.01~0.55ppm), in 16 items of meat-eating manufactured foods 30 items (0.04~0.18ppm), in 20 items of snack cookies 28 items (0.09~1.43ppm), in 8 in roasted oil foods 10 items (0.04~1.22ppm), in 6 items of peanut processed food 10 items (0.06~0.25ppm), in 1 of vegetable cream 15 items (0.05ppm). Detected level was lower than the result of monitored by other countries.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.117-124
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• 1984

Sarine scrap usually comprises about $40\%$ of the raw fish in processing. The purpose of this study is to establish the desirable methods for proteinaceous materials from the sardine scrap through autolysis or enzymatic digestion and to convert them into useful by-products such as sardine sauce. Sardine scrap was chopped and mixed with equal weight of water, and be hydrolyzed them by autolysis and/or by addition of commercial proteolytic enzyme and various concentrations of sodium chloride. The optimal conditions for hydrolysis of sardine scrap were revealed in temperature at $55^{\circ}C$ and 4 hours digestion with bromelain($0.4\%$) and commercial complex enzyme ($6.0\%$), and those conditions were also applicated in autolysis. The maximum hydrolyzing rate of protein and released amino nitrogen were $82.5\%,\;5.2\%$ through autolysis, $84.3\%,\;5.8\%$ by bromelain digestion and $92.5\%,\;5.9\%$ by complex enzyme, respectively. In the products prepared from sardine scrap through autolysis or bromelain digestion, hypoxanthine was dominant, as $17.4 {\mu}mole/g$, dry matter for autolysis and $16.0 {\mu}mole/g$, dry matter, for bromelain digestion among the nucleotidcs and their related compounds, respectively. The abundant free amino acids were leucine, glutamic acid, lysnie, valine and alanine. The contents of those amino acids were $51.3\%,\;48.3\%$ of the total free amino acids, respectively. And the contents of 5'-IMP and TMAO were negligible but total creatinine was developed in value from $9.2\%\;to\;10\%$ of total extracted nitrogen. The flavor of sardine sauce prepared from sardine scrap by autolysis or enzyme digestion were not inferior to that of traditional Korean soy sauce by sensory evaluation.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.99-100
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• 2000

어간장은 어패류를 자체의 자가소화 효소 및 미생물이 분비하는 소화효소에 의해서 제조되는 전통적인 수산발효 조미료로서, 최근 일부 회사에서 액젓 및 진국간장 형태로 상품화하고 있다. 또한 식염농도를 낮추고 제조기간을 단축하기 위한 방법으로서 단백질 가수분해 효소를 이용한 속성 제조를 가장 많이 사용하고 있으며, 이와 관련하여 제조방법, 조건 및 제품의 품질 형가에 관해서 많은 연구가 수행되어 왔다. (중략)

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.157-157
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• 1993

TMI는 알라닌과 글리신이 풍부한 18개의 아미노산으로 이루어져 분자량이 약 1,400Da인 물질이었으며, 가수분해 효소 처리후 억제력의 변화를 보이지 않았고, Porcine liver, spleen, testis에서 정제한 PM II에 대하여 TMI가 비상경적 억제작용을 하는 것으로 나타났으며, Ki value는 각각 300 nM, 250 nM 297 nM이었다. 그리고 돼지 간장에서 부분정제한 PM I, PM II, PM III, phospholipid methytransferase에 대한 정제된 TMI의 영향을 조사해 보면 각각 33%, 47%, 31%, 35%의 억제효과를 나타내었다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.399-408
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• 1998

The effect of low molecular alginates feeding on the cholesterol levels and fatty acid compositions of rat serum and liver lipid were investigated. After one week basal diet feeding, four week old Sprague-Dawley male rats were fed with water soluble and acid $\cdot$alkali soluble alginate extracted from sea mustard (Undaria pinnatifida) and giant kelp (Macrocystis pyrifera), and their low molecular alginates prepared by the HCl partial hydrolysis. The feeding efficiency of the alginate fed group was ranged in 0.37$\~$0.44, which was 0.03$\~$0.05 lower than that of the basal diet group. Also, there was much less increase of liver weight in the alginate fed group. The water soluble alginate showed more significant effect in reducing the total cholesterol, free cholesterol, LDL-cholesterol, triglyceride and phospholipid of serum and liver lipid than the acid$\cdot$alkali soluble alginate. The effect was much better with low molecular alginate (reducing effect by the low-molecularization : Water soluble alginate - serum lipid; total cholesterol $59\%$, free cholesterol $65\%$, LDL-cholesterol $96\%$, triglyceride $50\%$, and phospholipid $36\%$. liver lipid: total cholesterol $4\%$, free cholesterol $62\%$, LDL-cholesterol $44\%$, triglyceride $33\%$, and phospholipid $44\%$. acid$\cdot$alkali soluble alginate - serum lipid; total cholesterol $52\%$: free cholesterol $97\%$, LDL-cholesterol $78\%$ triglyceride $32\%$, and phospholipid $64\%$. liver lipid; total cholesterol $11\%$, free cholesterol $12\%$, LDL-cholesterol $10\%$, triglyceride $27\%$, and phospholipid $21\%$). The effect of low molecular alginate feeding on the fatty acid composition of serum and liver lipid reflects the remarkable increase of polyenoic acid, over $44\%$ in serum lipid and about $70\%$ in liver lipid, comparing to the cholesterol fed group. The overall results indicated that feeding of low molecular alginates improves physiological function of rats by changing the serum and liver lipid composition.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.176-182
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• 1999

Whole soybean cereal was fermented with four fungal strains (Mucor and Rhizopus) in pilot meju fermentation system. The pH range of the fermented soybean cereal was $7.16{\sim}8.38$, the contents of reducing sugar and amino-nitrogen were $0.54{\sim}2.64%,\;93{\sim}312mg%$, respectively, and that of free fatty acid ranged $0.06{\sim}2.00%$. The components of the amino acid, organic acid, free sugars and fatty acid showed distinctive patterns among four groups of fermented soybean cereals. Amylase activity and carbohydrate degradation rate of R. oryzae, protease and protein degadation rate of R. stolonifer was higher than other strains. But lipase and lipid degradation rates of four strains were similar. The odor concentrates of the soybean cereals fermented with Mucor strains were similar to Aspergillus strains, but Rhizopus were possessed of the flavor components of Bacillus and Aspergillus. Soysauce, made from M. hiemalis and R. stolonifer fermented soybean cereal showed excellent sensory evaluation and it was proposed that the two strains will be useful in Korean soysauce process.

• KSBB Journal
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• v.18 no.6
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• pp.455-460
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• 2003

This study was designed to find out the rapid fermentation of soy sauce from koji hydrolyzates using air bubble column reactor packed with coimmobilized mixed culture system. Continuous ripid production was performed by coimmobilized Z. rouxiii BH-90 and C. versatilis BH-91. Coimmobilized cells of Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 mixture cells in the column reactor produced 2.8％ ethyl alcohol and 18mg/L 4-ethylguaiacol over 96 hours under the optimal conditions. Coimmobilized cells produced 2.30∼2.4% ethyl alcohol during 30 days, and decreased gradually from 40 days to 70 days. Also coimmobilized cells produced 4-ethylguaiacol at the constant rate of 16∼18mg/L and decreased gradually after 40 days. Final product of soy sauce contained 2.4% ethyl alcohol and 18mg/L 4-ethylguaiacol. However, amino acid compositions of soy sauce were consisted of predominantly glutamic acid, leucin, arginine, aspartic acid, Iysine and valine, which were more than 50% of total amino acid.

• Food Engineering Progress
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• v.21 no.2
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• pp.138-142
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• 2017

This study investigated the possible use of enzymatically hydrolyzed isolated soy protein (eHISP) to enhance the intensity of salty taste. The sodium chloride content of eHISP is 69.5 g/L. Yellowness (b) increased, and lightness (L) and redness (a) decreased with increasing eHISP concentration in sample solution. Also, perceived salty intensity of eHISP solution increased in sample solution with increasing added amount of eHISP with same NaCl concentration. The intensity of the salty taste was enhanced by 2-39% as the eHISP was added. The results suggest that it may be possible to reduce the content of sodium chloride in foods by enhancing the salty taste with eHISP.

• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1030-1035
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• 2002

Despite health benefits derived from fish oil, polyunsaturated fatty acids (PUFAs) contained in fish oil are susceptible to lipid oxidation. To determine the optimum condition for maintaining good quality cooked fish during storage, mackerels were broiled with salt or soysauce condiments, and the lipid oxidation during 12 days of storage at refrigerated condition was measured. Peroxide value of broiled mackerel group with salt significantly increased after immediate cooking and maintained higher value throughout the storage period compare to the soysauce-added group, but showed similar value to the control group. Conjugated diene content in the soysauce-group was lower than the control and salt-added groups. Malondialdehyde content of broiled mackerel increased twofold and showed similar values in soysauce-added and the control groups during storage, whereas increased in the salt-added group significantly. Fatty acid compositions of the three mackerel groups changed after cooking, whereas that of the control group was almost stable during storage. In comparison with raw mackerel, the ratio of PUFA and saturated fatty acids decreased significantly, and the content of n-3 family fatty acid decreased from 25.53 to 20.63% in salted broiled mackerel. Soysauce group showed no reduction of PUFA with increasing storage time and showed the highest ratio of n-3/n-6 among the three groups at 10 days storage. Results reveal soysauce condiment protects against lipid peroxidation of broiled mackerel. Maillard reaction products (MRPs) found in soysauce might be responsible for the inhibitory effect and is a good condiment for extending storage life of cooked fish containing high amount of PUFA.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.4
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• pp.326-335
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• 1988

In order to increase the availability of filefish scrap, the ordinary and low salt sauce were prepared, and identified their taste compounds in their products. To process the filefish scrap sauce, chopped filefish scrap was mixed with koji, 25% brine, slat and glucose (25.0 : 65.0 : 12.5 : 7.0, w/w) and fermented at $25{\pm}4^{\circ}C$ for 120 days. The same process was also carried out to process the low salt sauce adding sorbitol, lactic acid and ethyl alcohol (7.0 : 0.7 : 9.0. w/w) instead of salt. While amino nitrogen and volatile basic nitrogen(VBN) of products were decreased, pH and reducing sugar were increased all alone the fermentation period. The major free amino acids of products at final stage of fermentation were glutamic acid, alanine, leucine, lysine and aspartic acid. And the contents of total amino acid in the ordinary and low salt sauce were 4126.6(mg/100m1 sauce), 4519.5(mg/100m1 sauce) after fermentation. Hypoxanthine was revealed as the major constituent among nucleotides and their related compounds through fermentation. Free amino acid-N in the filefish scrap sauces were from 56.3%(ordinary) to 60.7%(low salted) of extractive nitrogen. From the sensory evaluation, the quality of products from filefish scrap sauce were almost equal to sold soy sauce on the market.