Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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A Study of Salty Enhanceability of Enzymatically Hydrolyzed Isolated Soy Protein

분리 대두 단백 효소가수분해물의 강도평가를 통한 짠맛증진효과 연구

  • Received : 2017.05.12
  • Accepted : 2017.05.15
  • Published : 2017.05.31
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Abstract

This study investigated the possible use of enzymatically hydrolyzed isolated soy protein (eHISP) to enhance the intensity of salty taste. The sodium chloride content of eHISP is 69.5 g/L. Yellowness (b) increased, and lightness (L) and redness (a) decreased with increasing eHISP concentration in sample solution. Also, perceived salty intensity of eHISP solution increased in sample solution with increasing added amount of eHISP with same NaCl concentration. The intensity of the salty taste was enhanced by 2-39% as the eHISP was added. The results suggest that it may be possible to reduce the content of sodium chloride in foods by enhancing the salty taste with eHISP.

소금의 과량 섭취가 성인병, 생활습관병 등 여러 건강상에 문제가 있다는 보고에 의해 이를 해결하기 위한 다양한 연구가 진행되고 있다. 우리나라도 전통적인 식생활 습관에 의해 많은 소금을 섭취하고 있어 이를 개선하기 위한 범국가적으로 노력하고 있다. 최근 콩단백질을 활용한 우리나라의 발효 식품인 간장 등이 짠맛을 증진한다는 보고가 있어 이에 대한 연구가 활발히 이루어지고 있다. 본 연구에서는 간장의 주원료인 콩단백질을 가수분해하여 얻어진 효소가수분해(eHISP)의 짠맛 증진 효과에 대해 살펴보았다. 동일한 소금농도의 용액에 eHISP의 첨가량을 달리하였을 때 첨가량이 증가할수록 짠맛 증진효과도 증가하여, 50 mmol/L의 용액에 2%의 eHISP를 첨가하였을 경우 $70.13%{\pm}5.45%$의 짠맛 강도를 인지하였으며, 용액의 소금농도가 낮을수록 eHISP의 첨가에 따른 짠맛 증진효과가 더 크게 나타났다. 이러한 짠맛 증진효과는 콩단백질의 주요 아미노산 성분인 glutamic acid, aspartic acid와 효소가 수분해에 포함되어 있는 arginyl dipeptide와 같은 저분자량의 펩타이드 등의 상호작용에 의한 것으로 보인다. 0.1%에서 2%까지 eHISP의 첨가량을 달리하였을 경우 최소 2%에서 최대 39%의 짠맛 증진 효과를 나타내어 분리대두단백질의 효소가수분해물이 짠맛 증진물질로서 활용 가능성이 있는 것으로 판단된다.

Keywords

References

  1. Braschi A, Gill L, Naismith DJ. 2009. Partial substitution of sodium with potassium in white bread : feasibility and bioavailability. Int. J. Food Sci. Nutr. 60: 507-521. https://doi.org/10.1080/09637480701782118
  2. Brown IJ, Tzoulaki I, Candeias V, Elliot P. 2009. Salt intakes around the world: implication for public health. Int. J. Epidemiol. 38: 791-813. https://doi.org/10.1093/ije/dyp139
  3. Chae HJ, In MJ, Kim MH. 1997. Production and characteristics of enzymatically hydrolyzed soy sauce by the treatment using protease. J. Korean Soc. Food Nutr. 26: 784-787.
  4. Choi JY. 2012. Sensory characteristics and synergistic effect from natural flavor enhancers in complex food system. MS thesis, Seoul Women's University, Seoul, Korea.
  5. Choi NE, Rho JS. 2013. Nobody not told the story about umami and MSG. Reebook, Paju, Korea. pp 25-140.
  6. Hwang YH, Cho HY, Kim KR, Lee SH, Choi MJ, Shin JK. 2015. Hydrolysis of isolate soybean protein using subcritical water. Korean J. Food Sci. Technol. 47: 772-778. https://doi.org/10.9721/KJFST.2015.47.6.772
  7. Jeong DH, Lee HC, Shim SK, Han BR. 2006. Fermented soy paste. Hongikjae, Seoul, Korea. pp 500-502.
  8. Jo YJ, Jeong YJ. Development trend of sodium reduction material. Food Ind. Nutr. 20: 8-12.
  9. Joo JH. 2002. Purification of antimicrobial peptide from hydrolysates of soybean protein. MS thesis, Chungnam University, Daejeon, Korea.
  10. KCDC. 2016. Korea health statistics 2015: Korea national health and nutrition examination survey. Korea Center for Disease Control and Prevention, Cheongju, Korea.
  11. Kearney PM, Whelton M, Reynlods K, Muntner P, Whelton PK, He J. 2005. Global burden of hypertension: analysis of worldwide data. Lancet 365: 217-223. https://doi.org/10.1016/S0140-6736(05)17741-1
  12. Kim HS. 2013. Baking properties and physiological characteristics of white pan bread treated with fermented soybean protein powder. MS thesis, Hanseong University, Seoul, Korea.
  13. Kim JS. 2016. A studies on the sensory characteristics and salty enhancing effect of enzymatically hydrolyzed isolate soybean protein. MS thesis, Jeonju University, Jeonju, Korea.
  14. Kim JW. 2012. A study on visualization of taste and color of tablewares. J. Korean Soc. Color Stud. 26: 107-119.
  15. Kim MH, Yoon SK, Kim HY, Park SK. 2007. Development of functional traditional food using hypoallergenic soy protein hydrolyzate. IPET report 105004-2, Kyonggi University, Suwon, Korea.
  16. Kim MR. 2010. Bitterness and solubility of soy protein, casein, gluten, and gelatin hydrolysates treated with various enzymes. J. Korean Soc. Food Nutr. 39: 587-594. https://doi.org/10.3746/jkfn.2010.39.4.587
  17. Kim YH. 2005. Studies on optimization of fermentation conditions for degradation of soyprotein. MS thesis, Jeonbuk University, Jeonju, Korea.
  18. Lee CH. 2001. Bitter peptide structure and characteristics of hydrolyzed soybean protein. J. Life Sci. 32: 3-41.
  19. Lee EH, Kim SK, Jeon JK, Cha YJ, Chung SH. 1981. The taste compounds in boiled-dried anchovy. Bull. Korean Fish. Soc. 14: 194-200.
  20. Lee JH, Noh SJ, Jeong YI, Lee Dh. 2007. Method of preparating composition comprising fermented soybean and peptide derived therefrom. Korea Patent No. 1007879490000.
  21. Lee MY. 2015. Reduced sodium contents of processed food. Food Ind. Nutr. 20: 1-5.
  22. Lim HJ, 2011. Sodium and nutrition problem. 179-183. In: Background information on dietary reference intakes and intake status for sodium and potassium of Korean. 2011 International Symposium and Annual Meeting, April 27, Ilsan, The Korean Society of Food Science and Nutrition. Seoul, Korea.
  23. McGough, MM, Sato T, Rankin SA, Sindelar JJ. 2012. Reducing sodium levels in frankfurters using a natural flavor enhancer. Meat Sci. 91: 185-194. https://doi.org/10.1016/j.meatsci.2012.01.018
  24. McNeely JD, Windham BG, Anderson DE. 2008. Dietary sodium effects on heart rate variability in salt sensitivity of blood pressure. Psychophysiology 45: 405-411. https://doi.org/10.1111/j.1469-8986.2007.00629.x
  25. Na BJ, Ha SD. 2009. Effectiveness and safety of salt. Food Sci. Ind. 42: 60-73.
  26. Park YW. 1993. Characteristics of the soybean protein and its utilization. J. Korean Soc. Food Nutr. 22: 643-649.
  27. Schindler A, Dunkel A, Stahler F, Backes M. 2011. Discovery of salt taste enhancing arginyl dipeptides in protein digests and fermented fish sauces by means of a sensomics approach. J. Agric. Food Chem. 59: 12578-1258. https://doi.org/10.1021/jf2041593
  28. Shin MG, Lee GH. 2010. Sensory and anti-oxidative properties of the spice combinations as salty taste substitute. J. Korean Soc. Food Sci. Nutr. 39: 428-434. https://doi.org/10.3746/jkfn.2010.39.3.428
  29. Shin MG, Song IS, Lee KH, Song SM, Kim CK. 2007. Natural plant spice for NaC substitute and prevention of oxidation. Korea Patent No. 1020070106056.
  30. WHO. 2012. Guideline: Sodium intake for adults and children. World Health Organization. Geneva, Switzerland.
  31. Yokotsuka T. 1986. Soy sauce biochemistry. Vol. 30. Academic Press Inc., Orlando, FL, USA. pp. 195-329.
  32. Yoon SJ. 2015. A studies on the sensory charateristics and salty enhancing effect of enzymatically hydrolyzed anchovy protein. MS thesis, Jeonju University, Jeonju, Korea.

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