• Food Science and Preservation
• /
• v.20 no.6
• /
• pp.877-885
• /
• 2013

Wine extracts of four different berry fruits, such as mulberry, blueberry, strawberry, and raspberry, were investigated for antioxidant, anti-tyrosinase, and ${\alpha}$-glucosidase activities by using in vitro assays. Additionally, quantitative changes of ${\alpha}$-glucosidase inhibitor in mulberry wine were determined by HPLC according to mulberry cultivars and fermentation process. Among four berry wines examined, mulberry wine showed the most potent ${\alpha}$-glucosidase inhibitory activity with 69.37% at 0.23 mg/mL, while blueberry and strawberry wines exhibited the strongest inhibition against DPPH radical and tyrosinase activity, respectively. Four compounds were isolated and purified from mulberry wine by a series of isolation procedures, such as solvent fractionation, and Diaion HP-20, ODS-A, and Sephadex LH-20 column chromatographies. Among them, Comp. 4 exerted the strongest ${\alpha}$-glucosidase inhibitory activity ($I_C_{50}=31.57{\mu}M$), and its chemical structure was identified as quercetin by UV and NMR spectral analysis. Finally, the "Daeseongppong" (16.83 ppm) muberry wine had larger amount of quercetin than the "Iksuppong" (14.85 ppm) and "Cheongilppong" (8.92 ppm) mulberry wines, but their contents of three mulberry wines decreased considerably with aging process. These results suggest that mulberry wine containing quercetin acted as ${\alpha}$-glucosidase inhibitor may be useful as a potential functional wine for improving diabetic disorder.

• Journal of Korean Society of Forest Science
• /
• v.107 no.1
• /
• pp.1-15
• /
• 2018

In order to determine the effects of thinning on mushroom fruiting, microclimate and air temperature in Quercus mongolica forests, this study investigated changes in crown density, soil temperature, air temperature and throughfall in the natural Q. mongolica forest which was thinned by approximately 45% four years ago. The results showed that crown density was smaller than control by 6% from April to October, The soil temperature and air temperature in the thinned stands were significantly higher than those of the control by $1{\sim}2^{\circ}C$ until August and differences in air and soil temperature. The average daily temperature difference was higher than control by $0.2{\sim}0.7^{\circ}C$ until October. Throughfall from July to September in the thinned was approximately 135 mm higher than in the control. The maximum difference in throughfall per unit time was 3.5 mm/h. Soil moisture in the thinned site increased by approximately 5% compared with the control and reduced to the normal moisture after 4 days in both sites. 55 mushroom species were found in the thinned area between July and September. The thinned site contained 10 mycorrhizal mushrooms more than the control and 1 saprophytic mushroom species more than the control. Shannon-Wiener Index was 3.2, approximately 0.5 higher than the control. Tylopilus neofelleus, etc., occurred in the thinned site more quickly, but Aaricus subrutilescens, Clitocybe sp, occurred later. In the thinned site, the dominance of Tylopilus neofellelus and Armillaria sp. increased approximately 6% and 30% and yield about 1.5 times and 20 times, respectively. In conclusion, thinning in the Q. mongolica forest increased the soil and air temperature, soil moisture, throughfall and the diversity of mushroom species, and advanced the period of occurrence, and increased dominance and yield of some mycorrhizal mushrooms and Amillaria sp.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2023.04a
• /
• pp.36-36
• /
• 2023

천마(天麻, Gastrodia elata Blume)는 난초과(蘭草科, Orchidaceae)에 속하는 식물로 잎과 뿌리가 없어 탄소동화능력이 없으며, 뽕나무버섯균과 공생하는 기생식물이다. 천마는 노지 재배에 따른 안정생산 문제가 지속적으로 발생하고 있다. 혹한, 폭우 등 기상환경에 따른 연차간 수량성 차가 673~1,175kg/10a로 크고, 연작에 따른 수량성이 연작 1회 시 29%, 연작 2회 시 68%가 감소하는 경향을 보이고 있다. 따라서, 본 연구는 천마의 연작에 따른 토양내 미생물상의 변화를 분석하기 위해 수행하였다. 본 실험은 초작지, 연작 1회지, 연작 2회지 및 자생지의 각각 3곳에 대한 토양을 Metagenome 분석법을 활용하여 미생물상을 분석하였다. metagenome 분석 결과, 초작지 3곳의 시료를 이용하여 확보한 총 read는 699,221개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,377개(0.2%), no hit, not assign된 read는 10,510개 (1.5%), Bacteria로 분류된 read는 342,916개(49.0%), Eukaryota로 분류된 read는 총 344,418개(49.3%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 3곳 포장에서 고르게 분포하고 있으며, 총 11,242 read로 전체의 3.3%를 차지하였다. 연작 1회지 3곳의 시료를 이용하여 확보한 총 read는 655,097개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,694개(0.3%), no hit, not assign된 read는 18,985개(2.9%), Bacteria로 분류된 read는 312,201개(47.7%), Eukaryota로 분류된 read는 총 322,217개 (49.2%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 3곳 포장에서 고르게 분포하고 있으며, 총 11,597 read로 전체의 3.6%를 차지하였다. 연작 2회지 3곳의 시료를 이용하여 확보한 총 read는 651,624개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 1,753개(0.3%), no hit, not assign된 read는 7,995개(1.2%), Bacteria로 분류된 read는 307,178개(47.1%), Eukaryota로 분류된 read는 총 334,698개(51.4%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 총 43,877 read로 전체의 13.1%를 차지하였다. 자생지 3곳의 시료를 이용하여 확보한 총 read는 731,719개였으며, 이 중에서 Eukaryota로 분류되지 않은 read는 2,828개(0.4%), no hit, not assign된 read는 585개(0.1%), Bacteria로 분류된 read는 356,690개(48.7%), Eukaryota로 분류된 read는 총 371,616개(50.8%)였다. 그리고 천마의 생육에 영향을 주는 Fusarium 속과 종은 MJH01 자생지에서만 전체의 0.2%(총 660 read)로 극히 일부가 분리되었다. 따라서, 천마의 생육에 영향을 미치는 Fusarium 속과 종은 초작지 3.3%, 연작 1회지 3.6%, 연작 2회지 13.1%, 자생지 0.2%로 나타났으며, 연작 2회 시 급격히 증가하는 것으로 판단되었다.

• Journal of Acupuncture Research
• /
• v.26 no.1
• /
• pp.91-98
• /
• 2009

목적 : 상지, 상백피, 상엽은 모두 뽕나무에서 유래한 것으로 한의학에서 수천 년 동안 사용되어왔다. 최근 이들의 미백효능이 발견되면서 더욱 주목을 받고 있다. 피부의 멜라닌 색소 침착은 자선에 대한 정상적인 방어기전이다. 그러나 비정상적인 색소침착은 심각한 미용적 문제를 가져온다. 최근 약침이 피부 미백에 이용되고 있다. 또한 발효와 열처리는 미백화장료 가공의 최신 경향이다. 이 연구에서는 상지, 상백피, 상엽 약침액이 발효와 열처리 후에 피부 미백에 어떠한 영향을 미치는지 알아보고자 한다. 방법 : 상지, 상백피, 상엽을 각각 4개의 그룹으로 나누었다. (1) 비발효, 비열처리, (2) 비발효, 열처리, (3) 발효, 비열처리, (4) 발효, 열처리한 그룹들에 타이로시나제 활성 및 도파 산화 활성, 그리고 멜라닌 함량을 측정하여 비교하였다. 결과 : 타이로시나제 활성 및 도파 산화 활성, 그리고 멜라닌 함량에 있어서, 발효, 열처리가 대체로 효과가 있는 것으로 나타났다. 특히 멜라닌 함량에 있어서 발효, 열처리한 군이 대조군에 비해 유의성 있는 감소를 보였다. 결론 : 주근깨, 흑색종, 간반(肝斑) 등 비정상적인 색소 침착 질환을 치료하기 위한 상지, 상백피, 상엽 약침액 제조에 있어, 발효 및 열처리 방식을 적용하면 보다 나은 미백효능을 발휘할 수 있을 것으로 생각된다.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.33 no.6
• /
• pp.349-355
• /
• 2019

The anti-inflammatory effects of 4'-O-methylalpinumisoflavone (OMAI) has been reported in recent years. To develop effective and safe skin whitening agents, we investigated the anti-oxidative and melanogenic effects of OMAI isolated from fruit of Maclura tricuspidata Carrière (Cudrania tricuspidata) in macrophage and melanoma cell lines. In our results, OMAI showed effective superoxide scavenging activity and suppressed production of lipopolysaccharide (LPS)-induced intracellular reactive oxygen species (ROS) in RAW264.7 cells. In addition, α-melanocyte stimulation hormone (MSH)-induced production of melanin was also reduced by OMAI in B16F10 cells. Finally, OMAI significantly inhibited tyrosinase activity in B16F10 cells. These results suggest that OMAI suppressed melanin production via scavenging reactive oxygen species and inhibition of tyrosinase activity.

• Journal of Sericultural and Entomological Science
• /
• v.32 no.2
• /
• pp.118-120
• /
• 1990

A mulberry field and 3 graftage nurseries in Puyo were flooded for 2 to 5 days in 1987 to investigate flood damage. The effect of fertilization upon fall yield in previously flood fields was also studied. The results were : 1. Graftage which received 2 days of flood were alive with decaying leaves submerged under water. Graftages submerged completely for 5 days died, whereas those whose top appeared above the water lived. Graftages which were knocked down by water and scratched by sand, following washing by a fire engine, died. 2. Mulberry trees flooded for 5 days were alive when tops were above the water. 3. Mud soil carried by the flood and deposited on the mulberry had a pH of 6.43, organic matter 2.4%, and available phosphorus of 124ppm. The original sandy soil of the mulberry field had a pH of 5.52, organic matter 0.3%, and available phosphorus of 467ppm. Mud, as a clayey soil with higth fertility, may play a role in soil building. 4. Mulberry from the flooded field showed 3.4% higher yield with additional fertilization than no fertilization. This suggests nitrogen a mobile element, was lost in the flood.

• Animal Systematics, Evolution and Diversity
• /
• v.3 no.2
• /
• pp.64-116
• /
• 1987

The purpose of the present paper is to record taxonomic notes on several Korean spider mites. The specimens on which this study is based were collected by the authors during the period from May, 1986 to June, 1987. The following species are the spider mites concerned in this paper. 1. Panonychus citri (McGregor), 2. Eotetranychus populi Koch, 3. Eotetranychus smithi Pritchard and Baker, 4. Shizotetranychus bambusae Reck, 5. Schizotetranychus celarius (Banks), 6. Shizotetranychus leguminosus Ehara, 7. Oligonychus orthis Rimando, 8. Tetranychus ranzawai Kishida, 9. Tetranychus phaselus Ehara, 10. Tetranychus trancatus Ehara , 11. Tetranychus vienensis Zacher, 12. Tetranychus urticae Koch . Among them, eight species with asterisk are newly recorded from Korea . In addition, empodiums of pretarsus were carefully observed and enzyme patterns by electrophoresis were compared on some species in order induce their systematic inter-relationship at either species or generic level.

• Journal of Sericultural and Entomological Science
• /
• v.25 no.1
• /
• pp.21-26
• /
• 1983

The mulberry variety, Jeonweon No. 1, has been cultivated in some parts of Ho-Nam District since the variety was selected at the Jeonbuk Provincial Silkworm Egg Production Station in the latter half of 1920s. However, the characteristics of the ariety have not been reported. Therefore, the cultivation experiment on the variety was carried out to investigate the characteristics of the variety at Jeonju and Suweon for four years from 1979 to 1982. 1. The mulberry variety, Jeonweon No. 1, belongs to the Morus alba L. and the sprouting stage is medium. 2. The branch growth of the variety was rather slow, however the number of branches was more and the posture of branches opened wide. 3. The rate of cold injury of shoot tips was a little high, but resistance to disease and the lodging of branches was weaker, compared with the Gaeryangbbong variety. 4. The annual yield of leaves of the variety per 10a was 1,474kg, 14% less than that of the Gaeryangbbong variety.

• Journal of Sericultural and Entomological Science
• /
• v.47 no.2
• /
• pp.62-67
• /
• 2005

Much attention has been focused on the activity of the natural antioxidants present in fruits and vegetables, because potentially these components may reduce the level of oxidative stress. Especially, mulberry leaves containing many natural components are considerable resource far natural antioxidants. The antioxidant capacity of mulberry leaves was investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The appropriate conditions for pre-treatments of mulberry leaves were 80% MeOH, 30 sec. vortex mixing. The antioxidant capacity of 16 varieties was 3303.4 nmol (ascorbic acid equivalents) at opening stage of five leaves in spring. The highest stage of antioxidant capacity (3708.0 nmol) and yield rate was just before the coloration stare with anthocyanin in fruits, whereas the lowest stage was middle of June (2231.6 nmol) and about two months growing stage after summer pruning (2064.6 nmol). But after summer pruning, the antioxidant capacity of mulberry.

• Culinary science and hospitality research
• /
• v.17 no.4
• /
• pp.285-294
• /
• 2011

Using mulberries, a kind of functional food rising these days, we made muffins that people often eat to find the best recipe for mulberry muffins. Also, to find their quality characteristics and show possibility of making new products using mulberries, tests of pH, specific gravity, specific volume, chromaticity, mechanical quality characteristics and preference were done. As more mulberry concentrate was added, pH of muffin dough was decreased and specific gravity was increased. Specific volume was decreased as more mulberry concentrate was added Weight became heavier while volume decreased; and it seems decrease of volume was caused by additives like roughage of concentrate. For chromaticity, all L, a, b values decreased. Acceptance test of muffins with 15% substituted sample showed the highest figure and the rest of the samples were similar to one another in general. For texture, hardness and chewiness were increased as more mulberry concentrate was added, but springiness and adhesiveness were decreased. For example, general interest was the highest in 20% added group, and the order is as follows; 20% > 15% > 10% > 0% > 25%. Addition of mulberry concentrate could lower the quality of products by decreasing their volumes and increasing their hardness. Therefore, this research suggests that the optimum addition rates of mulberry concentrate should be between 15~20%.