• Proceedings of the Korean Society of Toxicology Conference
• /
• 1998.10a
• /
• pp.8-13
• /
• 1998

Polyoma Virus Enhancer core Binding Protein (PEBP2)는 유전자의 전사를 조절하는 hetero-dimeric transcription factor로서 $\alpha$와 $\beta$ subunit으로 구성되어 있다. $\alpha$ subunit을 coding 하는 유전자중 하나인 PEBP2aB는 급성백혈병과 관련되어있는 t(8;21) 또는 t(12;21)에 의하여 변형됨으로서 백혈병 발병의 원인이 되고 있다 (Miyoshi et al., 1993; .Romana et al., 1995). $\beta$ subunit을 coding 하는 PEBP2$\beta$도 inv(16)에 의하여 변형됨으로서 백혈병을 유도하는 주요 원인이 되고 있다 (Liu et al., 1993). 이 유전자들의 생물학적 활성을 밝히기 위한 연구가 gene targeting에 의한 knockout mouse 생산 방법으로 수행되었다. 그 결과 PEBP2$\alpha$B와 PEBP2$\beta$ 유전자가 definitive hematopoiesis에 있어서 결정적으로 중요한 역할을 하고 있음이 관찰되었다 (Okuda et al., 1996, Wang et al., 1996a, 1996b), 이는 이들 유전자가 bematopoietic master switch 유전자임을 밝힌 중요한 결과로서 이로부터 혈액학 연구 분야의 새로운 장이 열리게 되었다. 또한 이러한 연구 결과들은 PEBP2 family에 속하는 다른 유전자의 생물학적 활성의 연구를 촉진하는 계기가 되었다. 최근 PEBP2$\alpha$A 유전자가 결손된 마우스가 생산되었는데 이 유전자의 경우에는 모든 종류의 뼈의 생성이 완전히 결손됨이 관찰되었다 (Komori et al., 1997). 이는 PEBP2$\alpha$A 유전자가 뼈의 생성을 지배하는 master switch 유전자임을 보여주는 중요한 관찰로서 bone biologist 들의 큰 관심을 모으고 있다. 본 연구팀은 PEBP2 family 유전자 중 유일하게 아직 생물학적 활성이 규명되지 않은 PEBP2$\alpha$C 유전자의 활성을 knockout 마우스를 생산하는 방법에 의하여 분석하였으며 소화기관의 형성에 중요한 역할을 하고 있음을 확인하였다.

• Korean journal of food and cookery science
• /
• v.18 no.6
• /
• pp.698-703
• /
• 2002

The objective of this study was to determine the effects of wine and different meat bones added in brown sauce. Four kinds of brown sauce were prepared to find the changes of physiochemical characteristics, such as pH, $\alpha$-amylase activity, color, viscosity and microorganisms in brown sauce during storage at 4$\^{C}$. The pH of 4 kinds of brown sauce ranged from 5.0 to 5.6, and these pH ranges were stable during storage period. The activity of $\alpha$-amylase was the highest in the brown sauce made with wine and some of chicken bones and the sauces stored for 2 ∼ 6 days. The brown sauce made with wine and some of chicken bones had 35.5% of reducing sugar, which was the highest among 4 kinds of brown sauce, and had the darkest color. Also the brown sauce with wine and some of chicken bones shooed low values of L, a, b after storage and the lowest viscosity among 4 kinds of brown sauce. But they all tended to have higher viscosity during storage, especially two days after. The sauce made with wine and some of chicken bones showed the least change of viscosity and the addition of wine in the sauce appeared to inhibit the microbial growth.

• Journal of Food Hygiene and Safety
• /
• v.37 no.5
• /
• pp.364-371
• /
• 2022

The bones of the human body support the structures of the body and provide protection for a person's internal organs. Bone metabolic diseases are on the rise due to a significant increase in life expectancy over a short period of time. Therefore, we investigated the osteoblast differentiation promoting and osteoclastogenesis inhibitory activities of fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf). We evaluated the alkaline phosphatase (ALP) activity of MC3T3-E1 mouse calvarial-derived osteoblasts. We also evaluated expression of ALP, osteocalcin (OCN), and runt-related transcription factor 2 (Runx2), which regulate osteoblast differentiation. To assess effects on osteoclast formation, tartrate-resistant acid phosphatase (TRAP) activity in RAW264.7 cells was analyzed. ALP activity increased by 121-136% and 140-156%, respectively in the presence of HR1901-BS and HR1901-BSaf. Expression of osteoblast differentiation factor also increased significantly. We also confirmed that HR1901-BS and HR1901-BSaf decreased TRAP activity in osteoclasts by 35-47% and 23-39%, respectively. Our results showed that fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf) increase bone mineralization and osteoblast differentiation activity in MC3T3-E1 cells, and inhibit bone resorption activity in RAW264.7 cells. In conclusion, fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf) can be used as an effective natural resource for preventing and treating bone-related diseases.

• Nuclear Engineering and Technology
• /
• v.12 no.1
• /
• pp.19-28
• /
• 1980

An instant labelling technique for lyophilized pyrophosphate with $^{99m}$Tc is described labelling yield of about 90% is obtained at the pH range 3.5-5.5 on reconstitution with sodium pertechnetate-$^{99m}$ Tc solution. The final product is controlled by a modified two dimensional paper chromatography using 85% methanol and 0.85% saline, and biodynamic investigations are performed on white mice. Generally, the less amount of stannous chloride is used. the higher labelling yield is obtained. The molar ratio of pyrophosphate to stannous chloride of 10 : 1 ~ 50 : 1 is sufficient. The more amount of reduced unbound $^{99m}$Tc is injected, the more radioactivity is incorporated in the liver. Thus. the cause of the false bone-imaging is attributable to the presence of reduced unbound $^{99m}$Tc which is known to be well adsorbed to oxidized tin colloidals. The maximum uptake ratio of bone: liver in mice, in weight basis, 35 : 1 is achieved in lime of 60 min. or so. Tile preparation is suitable for clinical investigations on patients with bone diseases.iseases.

• Journal of Environmental Impact Assessment
• /
• v.25 no.6
• /
• pp.502-513
• /
• 2016

In this study, heavy metal distributions in the tissues of feral pigeon (Columba livia) were characterized using samples collected from bio-monitoring sites (Hangang Park and Hampyeong Park) of the NESB (National Environmental Specimen Bank), Korea, in order to evaluate the feasibility of feral pigeons as an indicator for the environmental monitoring. Cadmium (Cd) was analyzed to be accumulated in kidneys at higher concentration than in the other tissues. Such trend can also be found in the reviews on the Cd accumulations of the 34 cases including 17 avian species which showed that 31 cases had the highest Cd concentrations in the kidney among tissues. However, lead (Pb) was found to be richest in the bones in this study. 17 cases out of 30 reviewed cases had the highest Pb concentration in bones, whereas other 10 cases showed the highest concentration in kidneys, and 3 cases in livers. Therefore, kidneys together with bones can be a main target organ to test cadmium exposure to different habitat environments depending on physiological traits of birds. Zinc (Zn) was found to be the highest concentration in the pigeon livers of Hangang Park, but not in the bones. In contrast, the 13 cases of 16 reviewed cases had the highest Zn concentration in bones, and the 3 cases in livers. In addition, the heavy metal distribution patterns in relations to the metal accumulation mechanisms (a competition between Pb and Ca, a function of methallothionein protein, and etc.) were discussed.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2009.11a
• /
• pp.19.2-19.2
• /
• 2009

금속을 용해 응고시킬 때 생성되는 소위, 주조 결함이나 소결금속 내의 기공은 재료의 성능이나강도를 현저하게 낮추는 결함으로서 예전부터 기피되어 왔다. 또한, 재료공정에있어서도 여하의 기공이나 기포가 없는 치밀한 고강도 및 고기능성 재료를 개발하는 것에 최대한의 주의와 관심을 기울여 왔다. 그렇지만, 우리가 자연계의 천연물이나 인공물을 둘러보면 그 대부분이다공질임을 쉽게 눈치챌 수 있다. 예를 들어 목재, 지엽등의 생물을 시작해서 콘크리트 등의 인공물, 우리 체내의 뼈도 전형적인 다공질구조로 구성되어 있다. 이러한 구조로부터 재료의 재질제어 이외에 구조제어라는 새로운 어프로치를 고려할 수 있고, 최근 들어, 금속재료에 있어서도 이러한 다공질구조에 관한 연구가활성화되어 충격흡수재, 생체재료, 베어링재료 등의 다양한응용이 전개되고 있다. 특히, 원주상의 방향성 기공을 갖는 로터스금속은 기존의 복잡한구조의 다공질금속보다 뛰어난 기계적 성질을 갖는다. 이러한 다공질금속은 일방향응고할 때 생성하는 과포화가스원자를 석출시켜 기공을 일방향으로 성장시킨다. 즉, 융점에서의 고상과 액상의 가스 용해도 차를 이용하는 것으로서 응고시에 고용할 수 없는 가스원자가 기공을 형성한다. 이와같이 제조한 방향성 다공질금속은 BT (인플란트, 생체적합성, 저탄성, 경량), ST (초음속기엔진부품, 경량), IT (고성능수냉모듈), ET(고온촉매, 필터)의 분야로의 응용이 기대된다. 본 강연에서는 방향성 다공질금속의 제조법, 특성 및 응용을 포함하여그 동안의 연구성과 및 앞으로의 과제 등을 소개하고자 한다.

• Journal of the Korea Convergence Society
• /
• v.7 no.3
• /
• pp.177-184
• /
• 2016

The purpose of this study was to nutritional aspect of tofu. Tofu is a widely-enjoyed food made from soybeans, and it is a great example of how a simple food like soybeans can be woven into human food traditions in a way that is natural, inexpensive, and nourishing. One of the most important things to remember about tofu is its basic whole food nature. Tofu is produced with significantly less processing than most low-fat soymilks, it is a soy food that is much closer to a "whole foods" category than soy protein isolates and concentrates. From a health benefits standpoint, there are also benefits to tofu that has been fermented. Replacing meat and dairy with tofu and other soy products would also lower our total cholesterol intake by about 125 mg per day and our saturated fat by about 2.4 g per day. These nutritional changes, in turn, would lower our risk of several chronic diseases, including cardiovascular and cerebrovascular diseases.

• Ceramist
• /
• v.7 no.1
• /
• pp.47-60
• /
• 2004

골 대체재에서 생체활성재라 함은 이 물질이 체내에 식립 되었을 때 표면에 사람의 뼈와 물리적, 화학적으로 매우 유사한 성질을 갖는 저결정성 탄산 아파타이트가 스스로 형성되어 이 층을 매개로 하여 섬유성 조직의 생성 없이 신생골과 직접적으로 결합하는 능력을 갖는 물질을 말한다. 이와 같은 생체활성 물질은 1970년대 초 Hench가 Na$_2$O-CaO-SiO$_2$-P$_2$O$\^$5/ 글래스계에서 처음으로 발견하였으며 이를 Bioglass$\^$ⓡ/라고 명명한데서부터 유래한다. 그 후 결정성 아파타이트가 함유된 결정화 글래스인 Ceravital$\^$ⓡ/, 고결정성 아파타이트, 아파타이트와 wollastonite 결정을 포함한 Cerabone$\^$ⓡ/, 아파타이트와 phlogophite를 함유한 Bioverit$\^$ⓡ/, MgO-CaO-SiO$_2$-P$_2$O$\^$5/ 글래스, CaO-SiO$_2$ 글래스, ${\beta}$-tricalcium phosphate, 천연 calcite 등의 다양한 세라믹 물질이 생체활성을 갖는 것으로 보고되었다.(중략)

• Proceedings of the Korea Society for Simulation Conference
• /
• 2003.06a
• /
• pp.19-22
• /
• 2003

가상현실을 응용한 의료 시뮬레이션은 가상의 3차원 인체모델을 대상으로 위험부담 없이 정확하고 반복적인 훈련 및 교육을 할 수 있는 환경을 제공하기 때문에 의료 시뮬레이션 분야에 도입되어 효과적으로 활용될 수 있다. 본 논문에서는, 사용자와의 상호작용이 가능하고, 기존의 데스크탑 기반 모니터와 테이블 형태의 가상환경 보다 몰입된 가상공간을 체험할 수 있도록 해주는 CAVE$^{TM}$-like 시스템에서의 수술실 환경 프로토타입을 구현하였다. 이를 위하여, 인체의 주요 장기와 뼈를 삼차원 모델로 생성하고, 이 삼차원 모델과 동시에 이에 대한 이차원 단면 영상과 각각의 명칭 및 구조를 설명하는 해부도를 제공하도록 하였다. 또한 실제 수술실과 유사한 환경을 제공해 줌으로써 몰입감을 향상시킨다. 이에 덧붙여 VRML의 센서와 자바 스크립트를 사용하여 인터렉션을 제공하며 보다 현실감 있는 환경을 제공한다

• Journal of the Korea Convergence Society
• /
• v.8 no.11
• /
• pp.115-121
• /
• 2017

The present study aimed to obtain basic knowledge of a customized artificial joint based on the convergence research of Digital Imaging and Communications in Medicine(DICOM) and 5-axis machining technology. In the case of the research method, three-dimensional modeling was generated based on the medical image of the humerus bone, and the shape was machined using a chemical wood material. Then, the anatomical characteristics and the modeling machining computation times were compared. The results showed that the Stereolithography (STL) modeling required twice more time for semi-finishing and 10 times more time for finishing compared to the Initial Graphics Exchange Specification(IGES) modeling. For the 5-axis machining humerus bone, the anatomical structures of the anatomic neck, greater tubercle, lesser tubercle, and intertubercular groove were similar to those in the three-dimensional medical image. In the future, the convergence machining technology, where 5-axis machining of various structures(e.g., the surgical neck undercut of the humerus bone) is performed as described above, can be efficiently applied to the manufacture of a customized joint that pursues the precise model of a human body.