• Horticultural Science & Technology
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• v.18 no.3
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• pp.342-347
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• 2000

The objectives of this study were to investigate the genetic and phenotypic features of male sterile transformants by pollen-specific expression of diphtheria toxin gene and to find out inheritance patterns of transgene to the next generation. When backcrossed (BC) progenies were tested for expression of kanamycin resistance ($Km^R$), 9 lines out of 13 lines, except 4 lines ($BC_{1}5-13,\;BC_{1}5-23,\;BC_{1}5-28,\;BC_{1}5-32$), showed the ratio of $Km^R$ to kanamycin sensitive ($Km^S$), from 1:30 to all $Km^S$. As a result, they were much lower than Mendelian segregation of a dominant gene. To determine whether male sterility is a heritable and stable trait, 5 male sterile plants ($BC_{1}5-13,\;BC_{1}5-14,\;BC_{1}5-23,\;BC_{1}5-32,\;BC_{1}5-33$ lines) which had different transgene copy numbers were backcrossed as female parents with pollens from wild type. To confirm the existence of the DTx-A gene in the genome of the progenies, PCR was conducted using specific primers of the DTx-A coding region. A PCR band of 428 bp was obtained from each generation, which is the predicted size of the DTx-A gene fragment. Trangenes were inherited to the next $BC_4T_0$ progenies and showed male sterility, however, based on the copy numbers of DTx-A gene male sterile plants did not show predicted ratio. When male sterile plants were backcrossed with fertile plants, fruit capsule sizes and seed settings were relatively reduced from those of selfing wild type plants. The fruit sizes and seed settings were reduced in proportion to the increase in the copy number of DTx-A gene.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.59-68
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• 2011

Research was initiated to investigate the effect of high water-absorbing polymer on turf grass quality of three major turfgrasses. A total of 12 treatment combinations were used in the study. Treatments were made with different rates of sand, soil organic amendment (SOA), and water-swelling polymer (WSP). Visual turf grass quality was evaluated in creeping bentgrass (Agrostis palustris Huds., CB), Kentucky bluegrass (Poa pratensis L., KB), and zoysiagrass (Zoysia japonica Steud., Zoy) grown under greenhouse conditions. Significant differences were observed among the treatments in CB, KB, and Zoy. Visual quality ratings varied with mixing rates of SOA and WSP, being maximum 5.6 in differences among them. At the end of study it ranged from 0.3 to 9.6 in CB, 0.3 to 4.0 in KB, and 0.9 to 5.8 in Zoy. Turfgrass quality pattern changed with time after seeding among treatments influenced by WSP rates. From this study, a proper rate of WSP is considered to be 5%, 5~10%, and 5% for CB, KB and Zoy, respectively. In general, overall treatment effect of WSP on turfgrass quality was highly associated with SOA 20% in three turtgrass species. When mixing sand with SOA and WSP for rootzone soil, a proper rate of SOA is considered to be 15 to 20% for CB and KB, while 20% for Zoy of warm-season grass. A further study would be required to investigate the effect of varied, gradual mixing rates of WSP on growth characteristics of turfgrasses grown on mixtures of sand, SOA, and WSP before a field application.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.859-862
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• 2010

본 연구는 대전광역시의 응급의료서비스 취약지를 도출하고 취약지역의 보건학적 특성 및 응급의료서비스 취약여부와의 관련성을 분석하여 응급의료 관련 정책의 의사결정에 유용한 기초자료로 제공하기 위한 연구이다. 응급의료서비스 취약지 도출은 Arc GIS의 공간분석 방법 중 가중분석(Cost Weighted distance) 방법으로 응급의료센터로부터의 접근성 분석을 하였으며, 응급의료서비스 취약지의 보건학적 특성 및 응급의료서비스 취약여부와의 관련성은 SPSS 17.0을 이용하여 비모수 t-검정 및 다중회귀분석을 시행하였다. 본 연구의 주요 결과는 다음과 같다. 연구지역의 응급의료기관 분포는 동구와 유성구, 대덕구는 지정된 응급의료센터가 없으나 서구와 중구는 응급의료센터가 2개소 이상 위치하고 있어 응급의료기관 분포가 편중되어 있으며, GIS를 활용하여 응급의료센터와의 접근성 분석을 수행한 결과, 대전광역시 자치구별 전체 면적 대비 응급의료서비스 취약지의 비율이 높은 자치구는 동구가 41.2%로 가장 높았다. GIS를 활용하여 행정동별 응급의료서비스 취약지를 분석한 결과, 대덕구 신탄진동, 동구 대청동과 산내동, 유성구 구즉동과 노은2동, 서구 기성동, 중구 산성동으로 나타났으며, 응급의료서비스 취약지 중 기성동, 대청동이 노인 인구밀도가 높게 나타났다. 응급의료서비스 취약여부에 따른 보건학적 특성별 차이를 분석한 결과, 국민기초생활수급권자, 장애인등록자, 농업인구 비율의 평균은 취약지가 비취약지에 비해 높았으며 통계적으로 유의한 차이를 보였다(p<0.01). 응급의료서비스 취약여부를 종속변수로 하고 지역별 보건학적 특성을 독립변수로 하여 로지스틱 회귀분석을 시행한 결과, 농업인구 비율과 국민기초생활수급권자 비율이 높았으며 이는 통계적으로 유의하여 응급의료서비스 취약여부를 설명할 수 있는 변수인 것으로 나타났다(p<0.01, p<0.05). 이상의 결과를 종합하면 대전광역시 5개 자치구의 행정동 중 응급의료서비스 접근 불평등지역이 도출되었고 이러한 지역은 보건학적 특성 중 농업인구 비율과 국민기초생활수급권자의 비율이 높았으며 이는 통계적으로 유의하여 응급의료서비스 취약여부와 관련성이 있는 것으로 나타났다. 향후 효율적인 응급의료 자원 분배를 위해서는 GIS를 활용한 의사결정이 필요하며, 응급의료서비스 이용의 형평성을 증진시키기 위해서 응급의료서비스의 사각지대에 놓여있는 지역의 보건학적 특성을 고려한 정책이 시행되어야 할 것으로 사료된다.

• Research in Plant Disease
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• v.18 no.4
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• pp.324-330
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• 2012

This study was conducted to investigate the association of race distribution of Pyricularia oryzae with rice cultivar and location. Races present in a given location were different in cultivars, and the races from a given cultivars were different by location. For precise study of race distribution, it needs to identify isolates by considering cultivation ratio and collection area. There are great differences between the resistant degree in the upland blast nursery and on the resistant degree of the leaf blast and panical blast in the paddy field. The number of virulent races in a rice cultivar was not related with the resistant degree in blast nursery or in paddy field.

• Korean Journal of Environment and Ecology
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• v.15 no.2
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• pp.173-185
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• 2001

마편초과의 좀목형 엽에 함유된 물질의 allelopathy 효과를 구명하기 위해 수용성 엽 추출액, 메탄올 엽 추출액, 엽 분말과 토양과의 혼화된 pot에 각각 상치, 이탈이란라이그라스, 개솔새, 억새 종자를 발아실험하여 발아억제물질을 분석한 결과는다음과 같다. 1. 좀목형 엽 분말을 증류수에 각각 24, 48, 72 시간 침지 후 추출물에 상치, 이탈리안라이그라스, 개솔개, 억새 종자를 파종한 후의 발아율은 각 처리구마다 대조구마다 높은 발아억제를 보였고, 48시간 침지시에는 각각 26.9%, 58.8%, 6.6%, 22.2%의 발아율을 보여 강한 발아억제현상르 보였다. 2. 좀목형 엽 분말을 메탄올 75%에 24, 48시간 침지 후 추출물에 이탈리아라이그사스, 개솔새, 억새 종자를 파종한 후의 발아율은 48시간 추출액때 각각 33.3%, 15.4%, 7.7%를 보여 48시간 수용성 추출액과 24시간 메탄올 추출액보다 강한 발아억제효과가 있었다. 3. 좀목형 엽 분말과 Vermiculite를 1: 99.5: 95, 10:90, 20:80(w/w)으로 구분한 pot에 공시수종을 파종한 결과 엽 분말의 비율이 높을수록 발아억제효과가 컸으며, 엽 분말이 20%인 경우 발아된 부분의 건중량의 비율은 실험구와 대조구간에 비교한 발아율의 비율보다 훨씬 낮아 생장억제효과가 있는 것으로 나타났다. 4. Column chromatography의 TLC를 이용하여 최종 7 fraction을 찾고 생물검정 결과, 5, 6번 fraction에 강한 활성을 나타냈다. 5. UV 와 IR분광분석 결과 UV 분광분석은 256 nm과 336nm에서 흡수 최대를 나타내었으며, 이들은 시르시트시약을 사용하여 검토한 결과 7위에 에테르 결합한 전형적인 플라본류로 추정되었고, IR 분광분석 결과 지방족에 에테르결합이 존재하는 것으로 생각되었다. 6. Py-GC-MS 분석으로부터 5, 6 fraction의 경우 플라본류에 1개의 당이 결합한 배당체 구조이고 B환의 경우 1개 또는 2개 이상의 페놀성 수산기의 존재가 예상되었다.

• Korean journal of applied entomology
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• v.27 no.2
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• pp.63-67
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• 1988

Biotypes of the brown planthopper(BPH), Nilaparvata lugens St l, population migrated from maninand China in to southern coastal regions of Korea were investigated in 1985-1987 by honeydew excretion test using Korean cultvars,, 'Milyang 23'(no resistant gene), 'Cheongcheo-ngboy'(bph 1 gene), 'Milyang 63'(bph 2 gene) and 'Gayabyeo'(bph 1 and bph 2 gene). There was a correlation between the amount of honeydew excreted and the ability of each biotype to infest resistant cultivars: 'Milyang 23' with no resistant gene was highiy susceptible to bioty-pe-1, 2 and 3 ; 'cheongcheongbyeo' with resistant bph 1 was susceptible to biotype-2: and 'Milyang 63' with resistant bph 2 gene was highiy resistant to biotype-1, 2 and 3. Percent biotype dis-tribution of the female BPH collected in the southern coastal regions of Korea showed that in 1985, 1986 and 1987, the BPH biotype-1 was predominant accounting for 64.7%, 61.2% and 57.9%, respectivley, whereas bitype-2 was 22.0%, 21.9% and 29.7%, and biotype-3 was 13.3%, 17.0% and 12.6%, repestively.

• Management & Information Systems Review
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• v.32 no.5
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• pp.287-300
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• 2013

In this paper, price discovery between spot and futures in crude oil markets investigated using the Gonzalo and Granger and Hasbrouck common-factor models. The main findings are as follows. 1) Crude oil futures and spot market are cointegrated. 2) Following the preceding studies, we judged that Dubai(WTI) futures markets contribute to the price discovery process than Dubai(WTI) spot market when this Gonzalo-Granger and Hasbrouck information ratio for Dubai(WTI) market are larger than 0.5. In other words, the futures markets of Dubai and WTI plays a more dominant role in price discovery than the spot market. 3) But Brent futures market does not contribute to the price discovery process.

• Korean Journal of Psychosomatic Medicine
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• v.28 no.2
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• pp.116-125
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• 2020

Objectives : Self-poisoning is the leading cause of visits to the emergency departments after a suicide attempts. This study is aimed to compare the patient characteristics according to the category of drugs ingested by the patients who attempted suicide. Methods : All medical charts were retrospectively reviewed from patients who visited the emergency center, at Seoul Medical Center, due to intentional self-poisoning from April of 2011 to July of 2019. We investigated the information regarding the subtype and quantity of the intoxication drug, how it was obtained, suicidal history, and psychiatric history, as well as, sociodemographic information. Variables were compared between prescription drug (PD) and non-prescription drug (NPD) poisoning groups. Results : The mean age of the NPD poisoning group was significantly lower than that of the PD poisoning group. The patient ratio of those enrolled in national health insurance and living with spouses were significantly higher in the NPD poisoning group. Compared to the NPD poisoning group, the PD poisoning group had a higher incidence of mental illnesses, underlying diseases and ratio of involuntary visit to the emergency department. Among the prescription drugs, the benzodiazepine poisoning group had a higher rate of self-prescription than the non-poisoning group, while the zolpidem poisoning group had a higher rate of the using someone else's prescription than other drugs. Each single drug poisoning group (benzodiazepine, zolpidem, and antidepressant single-agent) had a higher rate of no mental illness than each of the mixed-poisoning group. Conclusions : Guidelines for regulating non-prescription drugs are needed as a matter of suicide prevention. Also, this study suggests that clinicians need to be careful when issuing prescriptions and should suicidal risk according to patients' characteristics, duration of follow-up and type of drug packaging.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.156-160
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• 2008

This study was conducted to find on the screening of a optimal variety after compare with characteristics of a colored soybean recommended and a vegetable soybean by pure line isolation from the indigenous variety in Chungnam area. The budding period in results compared with a budding property of testing varieties between the recommended colored soybean 6 varieties and the indigenous 3 varieties, was showed that it was delayed by increasing of a grain weight but the budding rate was not significant. And the harvest period was the most early day at the black colored soybean plot at the 30th July and was the latest day at the native variety (Nonsan jaerae) at the 21 th October. The stem length was showed a wide range from 25 to 104 cm, and the Asan jaerae in this result was the highest on 104 cm, but the Geomjeongolkong was the lowest on 25 cm. The number of pod per plant was searched about 9 to 111 range, and the most numerous plot in this experimental varieties was the Ilpumgeomjeongkong as 111 number, on the other hand, the least variety was the Asan jaerae as 9 number. The rate of more than 2 seed per pod in the vegetable soybean property was the highest at the Geomjeongolkong about 82%. Additionally, the Hwangkuemkong and Geomjeongkong 1 was relatively the high levels as 78% and 73%, respectively. At the pod yield per $1000\;m^2$ (10a) was the most much as 1,567 kg at the Ilpumgeomjeongkong. Also, the Sunheukkong and the Galmikong was 1,468 kg, 1,397 kg, respectively. Therefore, the Ilpumgeomjeongkong, Sunheukkong, and Galmikong were proper varieties for the vegetable cultivation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.10
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• pp.1594-1598
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• 2015

This study was performed to investigate the effects of various acidic treatment for anthocyanin and proanthocyanidin extraction from black bean. Extracts were prepared with 80% methanol solutions containing HCl, acetic, formic, phosphoric, and citric acids of different concentrations (0.1, 0.2, 0.3, 0.5, and 0.7%). Total anthocyanin content ranged from 0.74 mg/g in 0.7% citric acid to 1.74 mg/g in 0.3% HCl, depending on acid type and concentration. The major anthocyanins were delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), and petunidin-3-glucoside (Pt3G). The highest C3G content was 1.12 mg/g in 0.3% HCl. Proanthocyanidin content ranged from 2.01 mg/g in 0.5% acetic acid to 5.29 mg/g in 0.3% HCl. Thus, acidic condition is a significant factor affecting anthocyanin and proanthocyanidin extraction from black bean, and the optimum extraction conditions were determined as 80% methanol containing 0.3% HCl.