• Title/Summary/Keyword: 블루

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$In$ $vitro$ MRI and Characterization of Rat Mesenchymal Stem Cells Transduced with Ferritin as MR Reporter Gene (페리틴 리포터 유전자를 발현하는 백서 중간엽 줄기세포의 특성과 자기공명영상 연구)

  • Shin, Cheong-Il;Lee, Whal;Woo, Ji-Su;Park, Eun-Ah;Kim, Pan-Ki;Song, Hyun-Bok;Kim, Hoe-Suk
    • Investigative Magnetic Resonance Imaging
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    • v.16 no.1
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    • pp.47-54
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    • 2012
  • Purpose : This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate $in$ $vitro$ MRI detectability of ferritin-transduced RMSCs. Materials and Methods: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. $In$ $vitro$ magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. Results: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower $T_2$ relaxation time than non-transduced RMSCs. Conclusion: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.

Development of Wireless Ambulatory Measurement System based on Inertial Sensors for Gait Analysis and its Application for Diagnosis on Elderly People with Diabetes Mellitus (관성센서 기반의 무선보행측정시스템 개발 및 노인 당뇨 환자 보행 진단에의 응용)

  • Jung, Ji-Yong;Yang, Yoon-Seok;Won, Yong-Gwan;Kim, Jung-Ja
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.48 no.2
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    • pp.38-46
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    • 2011
  • 3D motion analysis system which is currently widely used for walking analysis has limitations due to both necessity of wide space for many cameras for measurement, high cost, and complicated preparation procedure, which results in low accessability in use and application for clinical diagnosis. To resolve this problem, we developed 3-dimensional wireless ambulatory measurement system based on inertial sensor which can be easily applicable for clinical diagnosis for lower extremity deformity and developed system was evaluated by applying for 10 elderly people with diabetes mellitus. Developed system was composed of wireless ambulatory measurement module that consists of inertial measurement unit (IMU) which measures the gait characteristics, microcontroller which collects and precesses the inertial data, bluetooth device which transfers the measured data to PC and Window's application for storing and processing and analyzing received data. This system will utilize not only to measure lower extremity (foot) problem conveniently in clinical medicine but also to analyze 3D motion of human in other areas as sports science, rehabilitation.

Design and Implementation of a Bluetooth Baseband Module with DMA Interface (DMA 인터페이스를 갖는 블루투스 기저대역 모듈의 설계 및 구현)

  • Cheon, Ik-Jae;O, Jong-Hwan;Im, Ji-Suk;Kim, Bo-Gwan;Park, In-Cheol
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.3
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    • pp.98-109
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    • 2002
  • Bluetooth technology is a publicly available specification proposed for Radio Frequency (RF) communication for short-range :1nd point-to-multipoint voice and data transfer. It operates in the 2.4㎓ ISM(Industrial, Scientific and Medical) band and offers the potential for low-cost, broadband wireless access for various mobile and portable devices at range of about 10 meters. In this paper, we describe the structure and the test results of the bluetooth baseband module with direct memory access method we have developed. This module consists of three blocks; link controller, UART interface, and audio CODEC. This module has a bus interface for data communication between this module and main processor and a RF interface for the transmission of bit-stream between this module and RF module. The bus interface includes DMA interface. Compared with the link controller with FIFOs, The module with DMA has a wide difference in size of module and speed of data processing. The small size module supplies lorr cost and various applications. In addition, this supports a firmware upgrade capability through UART. An FPGA and an ASIC implementation of this module, designed as soft If, are tested for file and bit-stream transfers between PCs.

Changes of Fresh Leaf Yield and Colorant Level with Different Transplanting and Harvest Time in Persicaria tinctoria H. Gross (이식 및 수확시기에 따른 쪽의 생엽수량 및 색소함량의 변화)

  • Ko, Jae-Hyung;Kim, Seong-Ju;Lee, Hoo-Kwan;Kim, Kwan-Su
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.2
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    • pp.177-184
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    • 2013
  • This study was conducted to determinate the optimum times of transplanting and harvest for enhancing the fresh leaf yield and colorant level of an indigo crop, Persicaria tinctoria H. Gross, containing the blue dye indigo. Two cultivars, Naju Local and a new cultivar, NaramBlue, were transplanted 5 times from May 30 to July 10 at an interval of 10 days, and all of experiment plots harvested on Aug. 23 in Muan, the south area of Korea. As transplanting time was delayed, fresh leaf yield were maximum at earliest transplanting (May 30) and then decreased, while Niram (blue dye extract) and indigo content of fresh leaf remained almost constant though showed a small variation. Also, two cultivars transplanted on May 23 were harvested 5 times from Jul. 20 to Sept. 20 at an interval of 15 days. As harvest time was delayed, plant height, No. of first branches, and fresh leaf yield changed increasingly, while Niram content was increased to Aug. 20 and then decreased slightly. Indigo level increased largely to Aug. 5, and then continuously decreased with more delayed harvest. These tendencies of changes in fresh leaf yield and colorant level with different transplanting and harvest times were shown similarly in both cultivars. The results indicate that early transplanting before May 30 and harvest in early August will be appropriate for improving fresh leaf yield and colorant level.

Enhanced Electrochemical Performance of NaxFe2(CN)6 Positive Electrode Materials for Lithium-ion Batteries (리튬이온 이차전지용 양극물질로서 NaxFe2(CN)6의 전기화학적 성능개선 연구)

  • Yoo, Seong Tae;Yoon, Seung Ju;Kang, Jeong Min;Kim, Haebeen;Ryu, Ji Heon
    • Journal of the Korean Electrochemical Society
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    • v.23 no.1
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    • pp.11-17
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    • 2020
  • The Prussian blue analogues of Fe2(CN)6 and NaxFe2(CN)6 are prepared by precipitation method and evaluated the electrochemical characteristics as positive electrode materials for lithium-ion batteries (LIBs) because of their low cost. Fe2(CN)6 shows a low reversible capacity of 34.6 mAh g-1, whereas sodium-containing NaxFe2(CN)6 exhibits a reversible capacity of 107.5 mAh g-1 when the discharge process proceeds first. When charging is first carried out to remove sodium in the structure, the reversible capacity of 114.1 mAh g-1 is achieved and the cycle performance is further improved. In addition, Nax-Fe2(CN)6 is synthesized at 0℃, room temperature (RT), and 60℃, respectively. Regardless of the synthesis temperature, NaxFe2(CN)6 shows similar initial reversible capacity, but the crystallite size is formed smaller and improved cycle performance when synthetic temperature is lower. The sample synthesized at 0℃ shows a reversible capacity of 86.4 mAh g-1 at the 120th cycle and maintains 76.8% of the initial capacity.

A Study on the Service Design for the Improvement of Living Service in the Temporary Residential Facilities for the Victims (이재민 맞춤형 실내 임시주거시설의 생활서비스 개선을 위한 서비스디자인 연구)

  • Kim, Chang Ryong;Kim, Jung Woo
    • Journal of the Society of Disaster Information
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    • v.16 no.3
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    • pp.526-541
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    • 2020
  • Purpose: The purpose of this paper is to identify and analyze the needs and complaints of the victims through the service design methodology and provide customized living services in indoor temporary residential facilities that can improve them. Method: The paper proceeded on the double diamond process. In the discovery phase, prior research analysis and surveys identified the needs and complaints of the victims. In the definition phase, persona and scenario were used to further clarify the problems of living service for indoor temporary residential facilities. In the development phase, wrote a service blueprint and found a section where problems occur during the service delivery process. In the delivery phase, compared the improved service with the existing service and proposed improvement plan. Result: Proposed service solutions that can improve privacy guarantee service, senior care service, and meal and laundry service problems. Conclusion: The proposed improvements are considered to provide improved living services in indoor temporary residential facilities.

Surface Modification of TiO2 Thin Films by N2 Atmospheric Plasma and Evaluation of Photocatalytic Activity (질소 상압플라즈마를 이용한 TiO2 박막의 표면개질 및 광활성 평가)

  • Lim, Gyeong-Taek;Kim, Kyung Hwan;Park, Jun;Kim, Kyoung Seok;Park, Yu Jeoung;Song, Sun-Jung;Kim, Jong-Ho;Cho, Dong Lyun
    • Applied Chemistry for Engineering
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    • v.20 no.4
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    • pp.402-406
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    • 2009
  • $TiO_2$ thin films were surface-modified with atmospheric plasma and their photocatalytic activities were evaluated. The films were deposited on glass plates by dip-coating in a $TiO_2$ sol-gel solution and sintered at various temperatures for various times. Nitrogen plasma was used for the modification and the experiments were carried out varying operational parameters such as discharge power and treatment time. Photocatalytic activity was evaluated based on the degradation efficiency of methylene blue (MB) under irradiation of UV-A and fluorescent light. According to XPS analysis, a little amount of nitrogen was found to be doped in the film surface after the modification. As a result, photocatalytic activity increased under irradiation of UV-A and fluorescent light, especially fluorescent light.

Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus (저온저장 음나무 배발생 캘러스로부터 체세포배 유도와 식물체 재생)

  • Lee, Na Nyum;Choi, Yong Eui;Moon, Heung Kyu
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.388-395
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    • 2015
  • Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at $4^{\circ}C$ in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at $4^{\circ}C$ without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

Status and Participation Plan of Economic Coorperation in Border Region of N.Korea and China (북·중 접경지역 경제협력 현황과 참여방안)

  • Yoon, Seung-Hyun
    • Land and Housing Review
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    • v.6 no.2
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    • pp.79-88
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    • 2015
  • North Korean leader Kim, Jung-Il visited Beijing, China, May 2010, when he made a common recognition with Chinese President Hu Jintao on construction of the Rasun SEZ and the Hwanggumpyong-Wyhwado SEZ for development of Economic cooperation between N.Korea and China and accelerating establishment of SEZs in N.Korea. However, after N.Korea's third nuclear test on Feb. 2013, the relationship between N.Korea and China became a little worse. Recently, three nations' border region near Rasun in N.Korea is reconsidered that it is very important place for collaboration between and among 2, 3 or 4 countries, S.Korea, N.Korea, China and Russia. This thesis examined these changes of cooperation and plans among the countries near the border region and proposed some measures for participation of S.Korea on the projects in the Rasun region.

Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $\textrm{CO}_2$ Concentration (스타티스 ‘오션 블루’의 자가영양배양시 광도, 환기횟수 및 $\textrm{CO}_2$ 농도가 소식물체의 기내 생육에 미치는 영향)

  • 정기원;정병룡
    • Journal of Bio-Environment Control
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    • v.11 no.3
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    • pp.115-120
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    • 2002
  • Growth and development of Limonium spp.‘Ocean Blue’plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $\mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$\pm$11$^{\circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $\mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ‘Ocean Blue’ plantlets.s.