• Korean Journal of Plant Resources
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• v.24 no.1
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• pp.17-22
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• 2011

This study was for developing leaf chlorophyll mutant cultivars of Cymbidium goeringii by ethyl-methanesulfonate(EMS) treatment. Chlorophyll mutant rhizomes were easily produced by 0.2% EMS treatment in this genus. Among the mutants, they became dark brown about 50% of the rhizomes. When the dark-brown rhizomes were cultured in a solidified MS medium, new rhizomes were formed from part of the old ones. Chlorophyll mutant rhizomes were obtained from subcultured meristem tissues of newly-formed rhizomes. The rhizomes were cut and subcultured for a year and then became mutant plants. As the results, they produced 4 kinds of leaf mutant cultivars; zigzag-striped, comb-striped, net-striped, and dwarf types, indicating that the EMS treatment in the rhizome could produce versatile leaf chlorophyll regulating mutants. These results suggest that our method is useful for developing leaf mutant cultivars of this planta which they are estimated as higher commercial values.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.73-78
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• 2021

We report a technique for obtaining seeds of 'Cheongnarae' (a variety of Hosta minor cultivated at the Korea National Arboretum in 2019) by artificial crossing of H. minor, collected from Jeju Island, as the female parent and H. minor 'Krossa Regal', cultivated outside Korea, as the male parent. Among the individuals obtained after sowing, those that showed different morphological characteristics, such as leaf shape and color, were selected. Among these individuals, those with thick leaves and light-colored flowers were further selected. Vegetative propagation and cultivation steps were repeated for evaluation of the characteristics. 'Cheongnarae' has relatively thicker leaves and a darker color (RHS 137A) than the control variety 'Black Hills' and has wings at the point where the petiole meets the leaf blade. The shape of the leaf blade edge is clearly sinuous, and the flower color is close to white (White N155B). 'Cheongnarae' cultivated in this way can be used for pot plant or gardening.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.217-222
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• 2010

Bark is a general medium for potted Cymbidium in Korea, but it is difficult to shake off or eliminate the medium from the roots before exporting the potted plants. This working process can injure the roots and then deteriorate plant marketability. This study was carried out to select the optimum medium easier to eliminate from the roots instead of bark and the optimum amount of applied fertilizer to improve the plant growth. Cymbidium young plants 'Honey Hot' and 'Desert Look' were planted in pots with bark, cocochip, and peatmoss. The plants were treated with 2, 4, and 6 g of slow release fertilizer. The plant growth characteristics were investigated in the first and second years during production period of three years. The medium characteristics and mineral nutrient content of the leaves were also examined in the second year. In the first year, the plant growth of 'Desert Look' was improved in all peatmoss treatments more than bark. 'Honey Hot' showed the highest plant growth values in the bark treatment. In the second year, the plant growth of the two cultivars was improved in peatmoss. Cocochip treatments showed the lower plant growth values than bark and peatmoss in the first and second year. There was no significant difference among fertilizer amounts in all the media. The higher CEC values of peatmoss medium resulted to higher capacity to hold more nutrients than bark, and the nutrient retention of the peatmoss improved the plant growth. The higher K and Ca contents in the leaves would contribute to improve the plant growth. Consequently, it would be possible to use peatmoss instead of bark for Cymbidium young plants, but there must be always attention to appropriately water the medium and manage the moisture.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.159-167
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• 2014

In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.

• Horticultural Science & Technology
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• v.18 no.1
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• pp.28-32
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• 2000

This study was carried out to investigate the effects of uniconazole treatment on the growth and flowering of potted Chrysanthemum indicum L. for high quality pot plant production. Uniconazole was drenched at 0.05, 0.01, or 0.15 mg a.i./pot at 14 days after planting (DAP) of rooted cuttings. Simultaneously the short-day treatment (SDT) and pinching were adapted. The same amount of uniconazole (0.05 mg a.i./pot) was spilt drenched at once, twice, and three times, respectively, at 1 week interval. Uniconazole markedly reduced plant height, branch length, and stem diameter. Plant height was reduced linearly with increasing uniconazole concentration at 0.05, 0.01, or 0.15 mg a.i./pot up-to 41.6%, 52.5%, and 58.5%, respectively. In 0.05 mg a.i./pot, the number of branches greatly increased and plant height of 22.6 cm was adequate for pot plant. However, higher concentrations (0.10, 0.15 mg a.i.) were not suitable for production of high quality pot plant (17.0, 14.8 cm, respectively). Pinching and SDT decreased the number of days to visible bud, while uniconazole treatments delayed days to visible bud by 5-9 days compared with pinching and SDT. Number of visible buds was highest at 0.05 mg a.i./pot uniconazole treatment. However, flower diameter was decreased by uniconazole treatment, resulting in compact form. Number of stomata was increased by uniconazole treatment. The length of vascular tissues of uniconazole-treated plants ($11.2{\mu}m$) was smaller than that of non-treated plants ($15.0{\mu}m$, and the size of xylem vessel was also decreased. Uniconazole treatment at 0.05 mg a.i./pot at 14 DAP with pinching and SDT were recommended for pot plant production of C. indicum L.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.449-455
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• 2010

'Moulinrouge' was selected as the best regenerating cultivar among 18 different spray-type chrysanthemum cultivars bred in the Gyeongnam Flowers Breeding Research Institute. When the leaf explants from standard- and spray-type chrysanthemum 'Jinba' and 'Moulinrouge' were incubated on MS basal medium supplemented with $0.5mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA, both 'Jinba' and 'Moulinrouge' induced adventitious shoots that can be regenerated into plantlets. Based on these regeneration conditions, we developed an efficient $Agrobacterium$-mediated chrysanthemum 'Moulinrouge' transformation method by using sequential selection of shoots from low ($10mg{\cdot}L^{-1}$) to high ($30mg{\cdot}L^{-1}$) concentrations of kanamycin after co-cultivation of leaf explants with $Agrobacterium$ for 10 days and induction of shoots. All kanamycin resistant plants investigated with genomic PCR analysis carried the report gene, $AtSICKLE$, in their genome. Although expression levels of the report gene in the transgenic plants investigated with RT-PCR were relatively low because of inefficiency of CaMV 35S promoter in chrysanthemum, transgenic lines expressing $AtSICKLE$ efficiently showed leaf epinasty phenotype. We expect that our results will provide a useful method that can perform a high-throughput investigation of genes isolated and studied well in model plants for molecular breeding of chrysanthemum.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.148-151
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• 2001

This study was conducted to investigate effect of activated charcoal on growth and yield of Scutelltaria baicalensis G. in different degree of activated charcoal consistency. The results obtained are summarized as follows. Stem length was shown the longest in activated charcoal 30%, and short in control. Also stem diameter was shown the thickest in activated charcoal 30%, and thin in control. Length of main root and thickness of main root were good in activated charcoal 30%. Quality and yield of Scutellaria baicalensis G. were the highest by activated charcoal 30%. Therefore, optimum consistency of activated charcoal was activated charcoal 30%.

• Journal of Bio-Environment Control
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• v.30 no.1
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• pp.65-71
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• 2021

This study was carried out to investigate the effect of the final irrigation timing (FIT) before packaging for long-term transportation on growth, flowering, and crop quality of Phalaenopsis after simulated dark shipping (SDS). Phalaenopsis Sogo Yukidian 'V3' plants grown in 11 cm-diameter plastic pots filled with potting media (sphagnum moss + bark or only sphagnum moss) were packaged in paper boxes for export at 3.5, 7, 10 days (FIT 3.5, 7,10; Experiment 1) and 4, 6, 8, 10 days (FIT 4, 6, 8, 10; Experiment 2) after the final irrigation and then stored in a growth chamber at 20 ± 1℃ and 70 ± 3% RH created for SDS. After 4 weeks, the plants were taken out and grown in a greenhouse at 23 ± 3℃ and 70 ± 5% RH, and crop characteristics were measured during cultivation. In Experiment 1, the survival rate of FIT 3.5 plants was lower than that of FIT 7 and FIT 10. There was no difference between treatments in days to first flower, the number of florets, and the elongation rate of flower stalks. In Experiment 2, the percentage of rotted leaves was lowest in FIT 6 when before forcing and at 12 weeks after forcing, and that of FIT 8 was similar to FIT 6 when before forcing, but slightly increased after 12 weeks. The percentage of rotted leaves of FIT 10 was highest and that of FIT 4 was also high. There was little difference in flowering characteristics among treatments. In conclusion, the FIT before packaging for long-term (4 weeks) transportation of potted Phalaenopsis 'V3' affected the leaf rot rather than the post-shipping growth and flowering. And it was considered appropriate to set the volumetric water content of the potting media just before packaging to about 30%.

• Journal of Life Science
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• v.29 no.11
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• pp.1281-1293
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• 2019

The rhizosphere is the active zone where plant roots communicate with the soil microbiome, each responding to the other's signals. The soil microbiome within the rhizosphere that is beneficial to plant growth and productivity is known as plant growth-promoting rhizobacteria (PGPR). PGPR take part in many pivotal plant processes, including plant growth, development, immunity, and productivity, by influencing acquisition and utilization of nutrient molecules, regulation of phytohormone biosynthesis, signaling, and response, and resistance to biotic- and abiotic-stresses. PGPR also produce secondary compounds and volatile organic compounds (VOCs) that elicit plant growth. Moreover, plant roots exude attractants that cause PGPR to aggregate in the rhizosphere zone for colonization, improving soil properties and protecting plants against pathogenic factors. The interactions between PGPR and plant roots in rhizosphere are essential and interdependent. Many studies have reported that PGPR function in multiple ways under the same or diverse conditions, directly and indirectly. This review focuses on the roles and strategies of PGPR in enhancing nutrient acquisition by nutrient fixation/solubilization/mineralization, inducing plant growth regulators/phytohormones, and promoting growth and development of root and shoot by affecting cell division, elongation, and differentiation. We also summarize the current knowledge of the effects of PGPR and the soil microbiota on plants.