• Title/Summary/Keyword: 분화상토

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Effect of Several Cultivation Condition on Growth of Brachythecium rivulare and Myuroclada maximoviczii (몇 가지 재배조건이 물가양털이끼와 쥐꼬리이끼의 생육에 미치는 영향)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.52-59
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    • 2013
  • This study was carried out to develop the proper cultivation methods of Brachythecium rivulare and Myuroclada maximowiczii which showed high-value for the interior landscaping and potting. Growth of two moss species cultivated in the compost covered with cloth was vigorous compared to that grown in containers only using cloth or compost, and their harvesting processes were easier. The growth and harvest easiness of mosses cultivated in compost were great rather than in bark or peatmoss. Compared to division, the spray of crushed mosses using mixer was effective for both gametophyte generation and their harvesting processes. In addition, the optimum inoculum for each container ($27{\times}17{\times}3cm$) was 2.0 g in B. rivulare and 4.0 g in M. maximowiczii. Overall growth of B. rivulare treated with nutrient solution (N:P:K=20:20:20) was inhibited compared to control, fresh-weight gain was reduced toward the higher concentration. But fresh-weight gain of M. maximowiczii was the highest with $0.25g{\cdot}L^{-1}$ treatment. Therefore, adequate moisture supply, after spraying crushed mosses (2.0 and 4.0 g each) in the compost covered with cloth, were the appropriate cultivation methods for B. rivulare and M. maximowiczii. Nutrient solution treatment with low concentration, during the cultivation period, would be the proper way only for M. maximowiczii.

Micropropagation and RAPD Analysis of Somaclonal Variants in Lavandula spica cv. Marino (라벤다의 기내증식과 RAPD에 의한 체세포 변이체 분석)

  • Li, Xian Ri;Seong, Eun-Soo;Kim, Il-Seop;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.2
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    • pp.94-100
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    • 1999
  • To establish the mass propagation system of Lavandula spica cv. Marino, shoot tip, node, internode and leaf segment cultures were carried out. RAPD was applied to detect the somaclonal variation. Callus induction was very high in the medium supplemented with 1 mg/l 2.4-D, 2 mg/l NAA. especially and combined with 0.05 mg/l BAP from leaves. Shoot formation was high with $2{\sim}4\;mg/l$ BAP or 4 mg/l BAP + 0.2 mg/l NAA from shoot tip. Shoot proliferation was 9.1 times in the $B_{5}$ medium with 0.5 mg/l BAP and 0.01 mg/l NAA. Root formation was improved in NAA, which was the concentration of 0.1 to 1 mg/l and 1 mg/l IAA. Nursery survival rate was enhanced over 90% and growth was looked good in the acclimation soil consisting of peatmoss : vermiculite : perlite (1:1:1, v:v:v). Randomly amplified polymorphic DNA banding patterns based on polymerase chain reaction (PCR) were used to assess the genetic variation in plants regenerated from in vitro culture.

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Multiple Shoot Induction from Radicle-derived Callus and in Vitro Propagation of Silene Acaulis Subsp. Arctics (극지식물 Silene acaulis subsp. arctica의 유근 유래 캘러스로부터 다신초 유도와 기내 증식)

  • Seo, Hyo-Won;Yi, Jung-Yoon;Park, Young-Eun;Kang, Sung-Ho;Chung, Ho-Sung;Kim, Ji-Hee
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.303-307
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    • 2006
  • We describe here an efficient in vitro propagation method of Silene acaulis subsp. arctica (Caryophyllaceae), one of the higher arctic angiosperms, through the multiple shoot regeneration after callus induction from the radicle. The seeds of S. acaulis subsp. arctica collected from Svalbard, the Norwegian Arctic, were germinated and calli were induced from the radicle on solid MS media supplemented with 0.25mg/L 2,4-D and 1mg/L $GA_3$ at both $10{\pm}1^{\circ}C\;and\;23{\pm}1^{\circ}C$ Two weeks after callus induction, the multiple shoots were efficiently regenerated on the MS media supplemented with 0.25 g/L BA and 0.05mg/L HPh. The total biomass increment of regenerated shoots increased most efficiently of S. acaulis subsp. afctica was showed the maximum efficiency in at $23{\pm}1^{\circ}C$ on 1/2 MS salt strength. The multiple regenerated plantlets of S. acaulis subsp. arctics were grown to normal plants on soil.

Effect of Culture Conditions on Pot Cultivation of Veronica longifolia L. (긴산꼬리풀의 분화재배에 미치는 재배조건의 영향)

  • Lee, Sang In;Yeon, Soo Ho;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.86-86
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    • 2019
  • 현삼과(Scrophulariaceae)에 속하는 긴산꼬리풀(Veronica longifolia L.)은 벽자색의 긴 꽃이 총상화서로 달려 청량감을 자아내어 분화소재로 이용한다면 단조로운 실내의 분위기를 환기할 수 있다. 따라서, 조경 및 관상용 식재에 용이한 긴산꼬리풀의 재배는 2018년 5월 15일에 파종하여 생산된 유묘를 2018년 7월 16일에 정식하여 2018년 10월 15일까지 수행하였다. 기비실험은 코트비료(싱싱코트)를 포트당 0, 10 및 20알 처리하였다. 추비는 hyponex (N-P-K, 4-6-6)를 4주 간격으로 총 3회 각 0, 1000 및 $2000mg{\cdot}L-1$로 엽면시비 하였다. 광량 실험은 무차광, 55 및 75% 차광막을 설치하여 수행하였다. 왜화제 종류 및 농도 실험은 dinizonazole(빈나리)과 daminozide (B-9)를 각 0, 1000 및 $2000mg{\cdot}L-1$ 농도로 처리하였으며, 적심처리는 4주차에 1회 처리 유무를 달리하였다. 모든 실험의 공통사항은 플라스틱 10호 화분에 원예상토를 충진한 후 200구 트레이에 종자를 셀당 4립씩 파종하여 생산된 유묘를 정식하였다. 연구의 결과, 기비처리는 초장, 마디수, 엽수가 증가하고, 절간장 및 측지수를 감소시켰으나, 유의적인 차이는 나타나지 않았다. 추비 처리는 농도가 높을수록 엽수가 증가하는 경향을 보였으며, $2000mg{\cdot}L-1$에서 무처리에 비해 유의적으로 많았다. 차광정도에 따른 긴산꼬리풀의 생육은 무차광 조건에서 생육이 가장 양호하였다. 차광률이 증가할수록 전반적인 생육이 억제되고, 개화율도 크게 감소하는 결과를 보였다. 왜화제 처리별로는 종류에 관계없이 전반적인 생육이 억제 되었다. Diniconazole 및 daminozide의 농도가 높을수록 초장이 억제되는 경향을 보였으나, 측지수, 마디수, 엽수도 감소하여 관상적 이용에 적합하지 않다. 적심을 할 경우 전반적인 생육은 큰 차이가 나타나지 않았으며, 측지수가 감소하였다. 따라서, 긴산꼬리풀의 분화생산을 위한 재배기술은 기비는 처리하지 않고, 추비는 $2000mg{\cdot}L-1$로 시비하며, 광이 충분한 곳에서 재배하는 것이 효과적이다. 또한, 왜화제와 적심은 일반적으로 줄기신장을 억제하고 마디수와 엽수를 증가시켜 관상적 이용효과가 있는 것으로 알려져 있지만 본 연구에서는 반대의 결과를 보여 처리 하지 않는 것이 효과적인 것으로 판단된다.

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Optimum Media of Sedum kamtschaticum for Pot Culture in an Ebb & Flow System (Ebb & Flow 시스템에서 기린초 분화재배에 적합한 배지 선발)

  • Kim, Su Jeong;Chang, Dong Chil;Suh, Jong Taek;Lee, Eung Ho
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.205-210
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    • 2008
  • Sedum kamtschaticum has been used as one of the native valuable plants for ornamental purpose. This experiment was conducted to find out the optimum media for pot cultured Sedum kamtschaticum using peatmoss and coir-based mixtures. Four mixtures such as peatmoss:perlite(5:5, v/v), peatmoss:perlite(7:3, v/v), coir:perlite(5:5, v/v), and coir:perlite(7:3, v/v) were used. Plants were irrigated with a Sonneveld solution in an ebb & flow system. The content of T-N, K, and Ca in the peatmoss-based media was higher than those of the coir-based media. The concentration of T-N, K, and Ca in the shoots of Sedum kamtschaticum increased when plants were cultured with peatmoss-based media. In the peatmoss-based media, plant growth, such as number of branches, leaf length and shoot fresh and dry weights, were significantly increased than those of plant grown in the coir-based media. As the results, the optimum media for pot cultured Sedum kamtschaticum in an ebb & flow system was considered to be the peatmoss-based mixtures.

Several Treatments of Veronica rotunda Nakai for Pot Cultivation (분화 재배를 위한 둥근산꼬리풀의 몇 가지 처리)

  • Yeon, Soo Ho;Lee, Sang In;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.89-89
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    • 2019
  • 둥근산꼬리풀(Veronica rotunda Nakai)은 다년생 초본으로 높은산 정상에서 자라며, 초장은 10~20 cm, 잎의 길이는 2~4 cm 정도이다. 또한, 기부의 잎은 엽병이 불분명하고, 줄기에는 털이 없는 것으로 알려져 있다. 관상소재로 이용되고 있으나, 식물정보는 매우 부족하며, 활용하기 위해서는 재배 연구와 식물에 대한 정보 수집이 필요하다. 이에 본 연구는 둥근산꼬리풀의 관상용 재배 시 적정 차광, 기비, 추비, 왜화제 및 적심 등의 처리 정도를 알아보고자 수행하였다. 재배방법의 확립을 위하여 선행연구에서 육묘한 유묘(2018년 5월 15일에 파종하여 생산)를 7월 16일에 정식하여 10월 15일까지 약 14주 동안 재배하였다. 공통조건은 플라스틱 화분 10호(이색포트, $9{\times}9cm$)에 원예상토를 충진하고, 200구 트레이에 셀 당 4립씩 파종하여 생산된 1셀을 정식하였다. 차광 정도 실험은 0, 55, 75% 차광막을 이용하였으며, 기비는 싱싱코트를 0, 10, 20알 처리하였고, 추비는 hyponex (N:P:K, 4:6:6)를 0, 1000, $2000mg{\cdot}L-1$의 농도로 엽면시비 하였다. 또한, 관상효과 증가를 위해 왜화제는 diniconazole, daminozide를 농도별(각 0, 1000, $2000mg{\cdot}L-1$)로 처리하였고, 적심은 4주차에 1회 처리 유무의 효과를 비교하였다. 연구의 결과, 둥근산꼬리풀에 기비 10알 처리는 생육에 유의적인 차이는 보이지 않았으나, 20알 처리는 무처리와 10알 처리에 비해 절간장, 경직경이 증가하는 결과가 나타났다. 추비는 절간장을 증가시켰으나, 측지수와 마디수는 유의적으로 감소시켰다. 차광처리별로는 55, 75% 차광 모두 무차광에 비해 측지수, 마디수, 엽수 및 개화율을 크게 감소시키는 결과를 보여, 둥근산꼬리풀는 광이 충분한 곳에서 재배하는 것이 적합한 것으로 생각된다. 왜화제의 처리는 종류와 농도에 관계없이 둥근산꼬리풀은 측지수, 마디수, 엽수를 감소시키는 결과를 보였다. 또한 초장이 짧아지고 경직경은 두꺼워졌으나, 유의적인 차이는 없었다. 종류별로는 daminozide $2000mg{\cdot}L-1$ 처리는 무처리에 비해 개화율이 증가하여, 둥근산꼬리풀의 분화용 재배시 daminozide $2000mg{\cdot}L-1$를 처리하는 것이 효과적이다. 적심처리는 무처리에 비해 전반적인 생육 및 개화를 억제하는 결과가 나타났다. 결론적으로 둥근산꼬리풀의 분화용 재배시 광량이 충분한 곳에서 재배하고, 기비와 추비는 절간장을 증가시키며, 측지수, 마디수, 엽수에 큰 차이가 없거나 감소하는 결과를 보여 관상용 재배에 적합하지 않은 것으로 판단된다. 추가적으로 daminozide $2000mg{\cdot}L-1$를 처리하며, 적심처리를 하지 않는 것이 좋을 것으로 생각된다.

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The Effect of Plant Growth Regulators on Regeneration Rate during Tissue Culture of Hop (Humulus lupulus L.) (홉(Humulus lupulus L.) 조직배양 시 재분화율에 미치는 식물생장조절제의 영향)

  • Tae Hyun Ha;Jun-Hyung Lee;So Young Yi;Si-Yong Kang
    • Korean Journal of Plant Resources
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    • v.37 no.4
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    • pp.431-438
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    • 2024
  • Hops enhance beer flavor and bitterness, acting as a preservative. In recent years, the booming trend of craft beer has prompted the introduction of foreign hop varieties for cultivation and production in South Korea. This study focuses on developing efficient in vitro culture condition of the hop (Humulus lupulus L.) variety 'Cascade' for treatments of plant growth regulators, i.e. IAA and Cytokinin. Using Auxin IAA and Cytokinin 2iP, Zeatin, BAP, and TDZ on MS medium as plant growth regulators, the experiment involved removing three nodes from the shoot apex. In vitro hop culture showed the highest shoot proliferation rate when only IAA was added, with approximately 21% higher compared to the combination with Cytokinin. Notably, IAA 0.1 mg/L + BAP 1 mg/L resulted in a superior shoot proliferation rate of around 91%. IAA 0.1 mg/L + BAP 1 mg/L was advantageous for shoot elongation. Callus induction occurred with TDZ, while control or IAA-only conditions exhibited shoot and root growth. Cytokinin addition led to callus formation and increased weight. Assessing survival and soil adaptation during in vitro hop seedling acclimatization involved maintaining near 100% humidity initially, gradually reducing it over three weeks. When transferred outdoors, 9 out of 10 seedlings acclimated successfully, confirming a 90% acclimatization rate.

Effects of Pot Soil and humidity on Growth for Transparent Cup Cultivation of Climacium japonicum Lindb (나무이끼의 투명컵 재배시 용토 및 습도가 생육에 미치는 영향)

  • Suh, Jong-Taek;Ryu, Seung-Yeol;Yoo, Dong-Lim;Nam, Chun-Woo;Hur, Youn-Young
    • Korean Journal of Plant Resources
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    • v.23 no.4
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    • pp.379-385
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    • 2010
  • This study was carried out to develop cultivation techniques of Climacium japonicum in transparent pot. We analyzed effect of different soil moisture content, air exposure degree, bed soil and ground cover moss on the growth of Climacium japonicum. The best humidity condition in transparent pot culture was 60~80% and a mixture of bed soil with peatmoss : perlite as 7:3 ratio was effective to growth. Two holes(each diameter is 4 mm) in the cover were also effective to the growth and control moisture in the transparent pot. The best mixture of bed soil was moss with peatmoss : pelite(7:3) in the pot culture but price of moss is expensive, the moss treatment was not practical. Total of 10 moss species were selected as ground cover plant; Hypnum erectiusculum Sull. et Lesq., Thuidium kanedae Sak., Hypnum plumaeforme Wils., Trachycystis microphylla Lindb., Bryum argenteum Hedw., Hypnum oldhamii Jaeg., Funaria hygrometrica Hedw., Leucobryum glaucum Aongstr., Polytrichum commune Hedw. and Weissia controversa Hedw. The plant height of selected moss was smaller than that of Climacium japonicum so these moss were very effective to cover the ground.

Effects of Seedling Container, Soil Composition, Seeding Rates and Fertilizer Conditions on Seedling Growth Characteristics of Elsholtzia byeonsanensis M. Kim (변산향유 유묘 생장에 미치는 파종용기, 토양조성, 파종량, 추비조건의 영향)

  • Lee, Sang In;Park, Ji Woo;Kwon, Ye Eun;Kim, Sang Young;Cho, Wonwoo;Jeong, Mi Jin
    • Korean Journal of Plant Resources
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    • v.35 no.1
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    • pp.29-35
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    • 2022
  • Elsholtzia byeonsanensis is a Korean native plant of the Lamiaceae family, and was first introduced in the Korean Journal of Plant Taxonomy. This plant has a short plant length of 20-35 cm, with leathery and glossy leaves. The purple flowers bloom in November, so they are highly useful as potted or ground cover ornamental plants. In this study, tray cell size (128, 162, and 200-cell trays), soil composition [horticultural soil (HS): decomposed granite (DG) 1:1, 1:2, and 1:4 (v:v)], seeding rate (1, 2, and 3 seeds per cell) and additional fertilizer concentrations (0, 250, 500, and 1000 mg/L Hyponex) were tested to find out the optimum seedling growth conditions. As a result of the study, E. byeonsanensis growth increased proportionally as the cell size of the tray increased. The growth was inhibited as the percentage of DG increased. As for the growth according to the seeding rate, plant height, plant width, leaf length, and leaf width decreased as the seeding rate increased, but there was no difference in the number of leaves, stem diameter, and root length. There was an increasing trend in seedling growth as the additional fertilizer concentration increased. Therefore, for producing E. byeonsanensis seedling, it is most effective to fill HS or HS:DG 1:1 in 128-cell trays, sow one seed per cell, and spray the seedling at 1000 mg/L for additional fertilizer.

Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor (홍경천 (Rhodiola sachalinensis A. Bor) 잎절편으로부터 식물체 재분화에 미치는 Cytokinin과 Putresine의 영향)

  • Bae Ki-Hwa;Lim Soon;Yoon Eui-Soo;Shin Cha-Gyun;Kim Yoon-Young;Kim Yun-Soo
    • Journal of Plant Biotechnology
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    • v.32 no.3
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    • pp.195-199
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    • 2005
  • The effects of cytokinin and putrescine on adventitious shoot induction from leaf explant of Rhodiola sachalinensis A. Bor were investigated. Among cytokinin used in this experiment, BA was more effective on adventitious shoot induction and shoot elongation than kinetin. Especially, 1 mg/L BA was the best to increase adventitious shoot induction (71%) and shoot elongation (3.0 mm). In addition, 100 mM putrescine in MS medium with 1 mg/L BA was higher in adventitious shoot induction (93%) and shoot elongation (3.8 mm) than single treatment of 1 mg/L BA. Adventitious shoots induced in this experiment rooted on 1/2 MS medium and acclimated over 95% on composed soil (peatmoss:sand=1:1).