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http://dx.doi.org/10.5010/JPB.2005.32.3.195

Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor  

Bae Ki-Hwa (Korea Ginseng Institute, Chung-Ang University)
Lim Soon (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Yoon Eui-Soo (Department of Biological Science, Kong-ju National University)
Shin Cha-Gyun (Korea Ginseng Institute, Chung-Ang University)
Kim Yoon-Young (Korea Ginseng Institute, Chung-Ang University)
Kim Yun-Soo (Korea Ginseng Institute, Chung-Ang University)
Publication Information
Journal of Plant Biotechnology / v.32, no.3, 2005 , pp. 195-199 More about this Journal
Abstract
The effects of cytokinin and putrescine on adventitious shoot induction from leaf explant of Rhodiola sachalinensis A. Bor were investigated. Among cytokinin used in this experiment, BA was more effective on adventitious shoot induction and shoot elongation than kinetin. Especially, 1 mg/L BA was the best to increase adventitious shoot induction (71%) and shoot elongation (3.0 mm). In addition, 100 mM putrescine in MS medium with 1 mg/L BA was higher in adventitious shoot induction (93%) and shoot elongation (3.8 mm) than single treatment of 1 mg/L BA. Adventitious shoots induced in this experiment rooted on 1/2 MS medium and acclimated over 95% on composed soil (peatmoss:sand=1:1).
Keywords
Adventitious shoot; BA; kinetin; putresine; Rodiola sachalinensis;
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Times Cited By KSCI : 2  (Citation Analysis)
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