• Title/Summary/Keyword: 분해

Search Result 35,057, Processing Time 0.065 seconds

Anaerobic Biodegradation of Lignin by BMP Test and Measurement of Lignin-derived Compound Using GC & GC/MS (BMP법에 의한 리그닌의 혐기성 분해 및 GC와 GC/MS을 이용한 리그닌 분해산물 측정)

  • Kim, Seog-Ku
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.16 no.3
    • /
    • pp.46-51
    • /
    • 2008
  • The traditional view of the fate of lignin under anaerobic conditions is that it is recalcitrant because molecular oxygen is required for depolymerization. The presence of lignin is apparently the most important factor affecting the biodegradability of ligneous materials. The initial step in the degradation of ligneous material to smaller intermediates is catalyzed by enzymes secreted by microorganisms and is generally regarded as the rate limiting step in the microbial mineralization of organic matter. Biochemical methane potential (BMP) test, typically used to assess anaerobic biodegradability of liquid wastes with added nutrients and bacteria, have been adapted to assess initial biodegradation of ligneous material under anaerobic conditions. A method based on selective inhibition of microorganism activity, by 3% toluene, has been used to measure using the initial degradation rate of ligneous material and the accumulation of lignin-derived compounds.

  • PDF

Effects of heavy metals on the degradation of fenitrothion, IBP, and butachlor in flooded soil (담수토양중(湛水土壤中)에 있어서 fenitrothion, IBP, butachlor의 분해(分解)에 미치는 중금속(重金屬)의 영향(影響))

  • Moon, Young-Hee
    • Applied Biological Chemistry
    • /
    • v.33 no.2
    • /
    • pp.138-142
    • /
    • 1990
  • The effects of heavy metals Cd, Cu, Cr, Ni, and Zn on the degradation of the insecticide fenitrothion (O, O-dimethyl O-4-nitro-m-tolyl phosphorothioate), the fungicide IBP (5-benzyl O, O-diisopropyl phosphorothioate), and the herbicide butachlor (N-butoxymetyl-2-chloro-2', 6'-diethylacetanilide) in flooded soils were examined in the laboratory. The degradation of the 3 pesticides in soil was greatly inhibited by the amendment of the 5 heavy metals. The inhibition rate was high in the order of butachlor>IBP>fenitrothion. Populations of fenitrothion-and butachlor-degrading microbes, which were counted by the MPN method, were lower in heavy metals added soil than in the control soil. The effect of heavy metals on the degradation of the 3 pesticides in soil varied with the kind and concentration of heavy metals and the kind of pesticides.

  • PDF

Microbial Decomposition of s-Triazine Herbicides, Atrazine and Simazine by a TNT-degrading Bacterium (TNT-분해세균에 의한 s-Triazine계 제초제인 Atrazine과 Simazine의 미생물학적 분해)

  • 오계헌;이명석;장효원;소재성
    • Korean Journal of Microbiology
    • /
    • v.36 no.3
    • /
    • pp.209-215
    • /
    • 2000
  • The purpose of U7is work was to iilvestigate the degradation of s-h~azine hel-hicidcs, ahilzine and simazine by TNT-degrader under several relevaut physicochemical environ~nental parameters. TNT-degrader showed effective degradability of atrazine and snnazine as well. Both atrazme (GO 1i1~11) and simazine ( 4 5 rng//) were completely degraded within 30 hrs and 4 days of incubation, respectively. As d ~ e concentrations of atrazine and sunazine increased in the media, the degradation ofthose compounds were delayed. Additional caubans were essential to degrade atrazine and simazule, and no degradation was achieved in the absence of additional carbons. The effect of supplemented nitrogens on the degradation of atrazine and sunazine was evalualed. Addition of a suppleinented nitrogen in he growth medium containing ah-azine or siinazine showed partial degr-adation olihose herbicides duriug the incubation period. However, complete degradation of atrazine and simazu~e was examined ul the absence or any supplemented nitrogens. Addltion of yeast extract in this study was inhibilory to atrazine aud siinazine degradations, respectively. TNT-degrader was a small Gram-negative cocco-bacillus. Physiological analysis using BIOLOG sysleln revealed that this strain was Ste~~ol~~opl~orno~~ns rrialtophilia.

  • PDF

호수 생태계의 생화학적 분석

  • 최승익;안태석
    • Korean Journal of Microbiology
    • /
    • v.33 no.1
    • /
    • pp.50-54
    • /
    • 1997
  • 이 글에서는 호수생태계에서 일어나는 변화 중 .betha.-glucosidase 활성도를 중심으로 생화학적인 분석과정을 통하여 유기물질의 분해, 생성, 소비등에 대한 과정을 설명하고자 한다. .betha.-glucosidase효소는 비교적 기질특이성이 넓고, glucose, celluhexose, carboxy-methylcellulose등의 .betha.고리를 가수분해시켜 monomer로 분해시키는 효소로서 수환경에서는 매우 중요하다. 즉 cellobiohydrolase에 의해 분해된 oligomer나 dimer를 세균의 세포막을 통과할 수 있는 크기로 분해시키는 효소이고, 이 효소의 분해 산물인 포도당은 세균에 의하여 바로 이용되고, catabolite inhibitor로 작용하므로, 이 효소 활성도의 높고 낮음은 수중생태계에서 탄소의 흐름과 세균 군집의 성장을 이해하는데 매우 중요하다(3).

  • PDF

Compound Noun Decomposition by using Bi-LSTM and Linear-chain CRF (양방향 LSTM과 선형체인 CRF를 이용한 복합명사 분해)

  • Lee, Hyun-Young;Kang, Seung-Shik
    • Proceedings of the Korea Information Processing Society Conference
    • /
    • 2018.10a
    • /
    • pp.719-720
    • /
    • 2018
  • 복합명사 분해 문제를 태그열 부착 문제로 정의하고 음절 임베딩과 딥러닝을 이용하여 복합명사를 분해하는 방법을 제안한다. 임베딩 방식으로는 음절 단위로 복합명사에 출현한 음절들을 벡터 공간에 표현하고 양방향 LSTM과 선형체인(linear-chain) CRF를 이용하여 복합명사 분해 태그를 부착하여 복합명사를 단위명사들로 분해하였다.

Korean Compound Noun Decomposition using Noun Bigram Model (명사 brigram 모델을 이용한 한국어 복합명사 분해)

  • Kang, Min-Kyu;Kang, Seung-Shik
    • Annual Conference on Human and Language Technology
    • /
    • 2010.10a
    • /
    • pp.9-14
    • /
    • 2010
  • 본 논문에서는 명사의 띄어쓰기 bigram과 단일명사 정보를 이용하여 복합명사를 분해하는 방법을 제시한다. 붙여쓰기와 띄어쓰기를 모두 허용하는 복합명사의 특징에 따라 띄어쓰기 bigram으로 후보를 선정할 경우, 분해시간과 후보의 수를 크게 줄일 수 있으며, 긴 음절의 복합명사도 bigram의 chain을 통해 빠르게 후보 조합이 가능하다. 분해 후보가 복수일 경우, 명사 간 bigram 확률을 계산하여 최적의 분해 후보를 선정한다.

  • PDF

Characteristics of Enzymatic Hydrolysates of Rice Bran and Rice Protein by Mixing Ratio and Hydrolysis Times (미강과 쌀 단백질의 비율과 분해 시간에 따른 효소분해물의 품질 특성)

  • Seon, Yoo Kyung;Goo, Hoo Mo;Park, Kwang Kun;Yang, Eun Ju
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.45 no.10
    • /
    • pp.1460-1466
    • /
    • 2016
  • This study was conducted to develop a savory ingredient using rice material. We made hydrolysates with ratios of rice bran and rice protein of 4:0, 3:1, 2:2, and 1:3 (w/w) using commercial enzymes, and then investigated their quality properties. At a ratio of 3:1, nitrogen degradation ratio (NDR), savory taste, and overall acceptability were the highest compared to other ratios. Rice bran and rice protein with a ratio of 3:1 were hydrolyzed for 13 days, and characteristics of the hydrolysate were investigated after 3, 5, 7, 10, and 13 days. Total nitrogen, amino nitrogen, and NDR of the hydrolysate after 10 days were higher than those of other hydrolysates. SDS-PAGE showed that the molecular weight of the hydrolysate peptide became smaller as hydrolysis time increased. Glutamic acid content was highest among all amino acids in the hydrolysate for 13 days. Amino acids related to bitter taste decreased from 5 to 13 days, whereas amino acids related to sweet taste substantially increased over time. Sensory evaluation showed that the hydrolysate after 10 days was best. These results suggest that rice bran and rice protein at a mixing ratio of 3:1 and hydrolysis for 10 days were optimal hydrolysis condition for development of natural savory ingredients.

Study on Exfoliation and Recovery of Anesthetized Yound Abalones, Haliotis discus hannai Treated with Ethy-p-aminobenzoate and Freshwater in Different Temperatures of Sea water (온도구간별 파라아미노안식향산에틸과 담수에 의한 양식산 참전복 (Haliotis discus hannai) 치패의 박리 및 마취회복에 관한 연구)

  • 최상덕;정성채;김호진;공용근;백재민;최규정
    • Journal of Aquaculture
    • /
    • v.10 no.3
    • /
    • pp.281-288
    • /
    • 1997
  • We investigated the optimal concentratin of ethyl-p-aminobenzoate for the exfoliation and recovery of young abalone, Haliotis discus hannai in according to different water temperatures, for the purpose of preventing the damage of shell and muscle to ecfoliated from shelter. In the 14$^{\circ}C$ water temperature, young abalones were exfoliated after 16, 35, 35 and 35 minutes in 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 100, 60, 30 and 30 minutes, respectively. Exfoliation rate of abalone were 100% except for 50 ppm (80%) and recovery rate were 100% of all concentration. In the $18^{\circ}C$ water temperature, young abalones were exfoliated after 4, 4, 6, 8, 8 and 12 munutes in 300, 200, 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 210, 180, 90, 60, 30, 20 and 20 minutes, respectively. Exfoliation rate of abalone were 100%, and recovery rate were 100% except for 200 and 300ppm (90%). In the $24^{\circ}C$ water temperature, young abalones were exfoliated after 8, 10, 10 and 12 minutes in 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 70, 50, 30 and 20 minutes, respectively. Exfoliation and recovery rate of abalone were 100%. In the 18$^{\circ}C$water temperature, exfoliation rate that treated with freshwater during 20 minute were 80, 50, 30 and 5% in 100, 75, 50 and 25% of fresh water, and recovery after 60, 15, 10 and 2 minutes, respectively and recovery arate were 100% except of r 100% freshwater. In this study, we suggest the reslults that the exfoliation and recovery by ethly-p-aminobenzoate were more effected in $18^{\circ}C\;and\;24^{\circ}C$ of sea water temperature than those of $14^{\circ}C$. The optimal concentration of ethyl-p-aminobenzoate was 50ppm at those water temperature. We raised 20 individual of young abalones at water temperature of $16^{\circ}C$ in the 1$\ell$ o ftnk and checked the variatin of dissolved oxygen (DO) by respiration of abalones that treated with 75ppm of ethyl-p-aminobenzoate. Before anesthetizion, DO were 6.17~6.20mg/$\ell$ and slowly decreased. But after 60 minutes, DO decreasing were stopped in 5.42~5.46mg/$\ell$. On the other hand, the control was continuously decreased and 5.27mg/$\ell$ after 60 minutes. The heartbeats of abalones were 33~45/minute in the water temperature of $18^{\circ}C$, but that treated with 100 ppm concentration of ethyl-p-aminobenzoate during 60 minutes, was 0/minute. And heartbeats of recovered abalones from anesthetizion were 29~43/minute.

  • PDF

Isolation of a Lignolytic Bacterium for Degradation and Utilization of Lignocellulose (Lignocellulose의 분해 및 이용을 위한 Lignin 분해 세균의 분리)

  • 김용균;김한수;김근기;손홍주;이영근
    • Journal of Life Science
    • /
    • v.12 no.4
    • /
    • pp.392-398
    • /
    • 2002
  • 38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphologcal and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LC-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.

A Study on the Condensation Modeling Method for Fast Pyrolysis Gas of Biomass (바이오 매스 급속 열분해 가스의 응축 모델링 기법에 관한 연구)

  • Park, Hun Chae;Choi, Hang Seok;Choi, Yeon Seok
    • 한국신재생에너지학회:학술대회논문집
    • /
    • 2011.11a
    • /
    • pp.107.1-107.1
    • /
    • 2011
  • 최근 석유, 가스, 석탄을 비롯한 화석연료의 다량 사용으로 기후변화, 대기오염 등의 환경문제 및 자원 고갈의 우려 때문에 바이오매스는 중요한 화석연료 대체 에너지 자원으로써 큰 관심을 받고 있다. 바이오매스 자원을 에너지로 전환하는 방법 중 하나인 급속 열분해 공정은 산소가 없는 상태에서 바이오매스를 열적으로 분해하여 액상 상태의 생성물을 회수하는 공정으로, 증기상의 열분해 가스를 응축하여 회수하게 된다. 바이오매스의 급속 열분해에 관한 연구는 주로 바이오매스의 종류와 열분해 조건에 따라 회수되는 바이오 원유의 수율 및 물리 화학적 특성에 관한 연구가 수행되고 있으나, 열분해 가스의 응축에 관한 연구는 응축에 수반되는 복잡한 물리적 현상 때문에 미진하다. 따라서 본 연구에서는 바이오매스의 급속 열분해를 통해 생성되는 증기상의 열분해 가스의 응축 현상을 모사 할 수 있는 모델링 기법에 대해 연구하였다. 급속 열분해 공정을 통해 생성되는 바이오 원유는 수백개의 화합물로 구성되어 있으며, 동일한 바이오매스를 사용한 경우라도 공정조건에 따라 바이오 원유에 포함된 화합물은 달라진다. 따라서 본 연구에서는 바이오 원유의 주요 화합물인 water, propanal, butanal, pentanal, phenol, guaiacol, coniferyl alcohol, formic acid, acetic acid, propanoic acid, butanoid acid를 대상으로 열분해 가스의 응축을 모사하였다. 본 연구에서는 응축 모델링 기법의 검증을 위해 실험결과와 비교하여 정확성을 검증하였으며, 본 연구의 결과를 활용하여 응축 조건 변화에 따른 급속 열분해 가스의 응축률을 예측하고, 이를 이용한 응축 열교환기 설계에 유용하게 사용될 수 있을 것으로 판단된다.

  • PDF