• Title/Summary/Keyword: 분해율

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Isolation of a Seaweed Hydrolytic Strain from the Microflora in Water of a Paddy Field (논물의 미생물군으로부터 해조분해능을 갖는 균주의 분리)

  • 김해섭;최옥수;강동수;박욱민;백승한;배태진
    • Journal of Life Science
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    • v.14 no.1
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    • pp.98-103
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    • 2004
  • Various bacterial strains were isolated from water in a paddy field, and their seaweed degrading activities were investigated. As the result, 16 strains were obtained from the microflora sample. They were incubated in a liquid medium of sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of the liquid medium were measured once a week during the incubation period. Ratio of reduced sugar to total sugar of 30A412 strain was highest. Accordingly, 30A412 strain was incubated in three different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week during the incubation period. Ratios of reduced sugar to total sugar and cell growth were increased with the incubation period. Cell growth and degrading ratio were highest in the liquid medium with sea mustard powder.

The Effects of Processing Methods of Corn on In sacco Starch and Protein Degradability in the Rumen (옥수수 가공방법이 In sacco 전분 및 단백질 분해율에 미치는 영향)

  • Son, K.N.;Kim, Y.K.;Lee, S.K.;Kim, H.S.
    • Journal of Animal Science and Technology
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    • v.45 no.3
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    • pp.421-432
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    • 2003
  • The objective of this study was to examine the effects of processing methods of corn grains on protein and starch degradability in the rumen by three ruminally cannulated dry Holstein cows. The corns for these experiments were untreated; whole corn L(density; 660 g/$\ell$), whole corn H(density; 740 g/$\ell$), and treated by four different types: Ground corn, 3.8 mm, 2.8 mm, and 1.5 mm flaked corn. The results obtained were summarized as follows: For 48 hrs, the protein degradabilities were high in order, ground corn, 1.5 mm, 2.8 mm, and 3.8 mm flaked corn(82.6, 76.5, 64.5, and 33.9%, respectively). Flaked corn grains were degraded lower than ground corn. However, as increasing the processing degree of flaking, the protein degradabilities, from 4 hrs to 48 hrs, were increased. The starch degradabilities on 48 hrs were higher in 1.5 and 2.8 mm flaked corns, ground corn, 3.8 mm flaked corn(99.1, 91.5, 89.5, and 68.9%, respectively) than whole corn L(32.0%) and whole corn H(20.5%)(P<0.05). By increasing the processing degree of flaking, the protein degradabilty between 2.8 mm and 3.8 mm was increased significantly from 68.9% to 91.5%, however, that of 1.5 mm flaked corn, processed thinner, tended to be increased slightly, but was not significantly different. From 12 hrs to 24 hrs, whole corn L was degraded little more than whole corn H in starch, was not significantly different. However, after 48 hr incubation in the rumen, whole corn L was degraded more 50% than whole corn H(P<0.05). The value of degradation parameter “a” of protein was lower in all flaked corns than in ground corn. In contrast, the value of degradation parameter “a” of starch was significantly higher in all flaked corns than in ground corn(P<0.05). It seemed that by flaking the corn grains, starch particles were gelatinized, and then, starch was degraded more rapidly, while protein was degraded more slowly. Referring to these kinds of physical characteristics of grain sources in ruminal degradabilities, it is possible to synchronize the fermentation of nitrogen and carbohydrate sources, in formulating the cattle diets.

Cellulose Degradation and Extracellulat Enzymatic Activity of the Mud Flat in Sunchon Bay (순천만 갯벌 토양의 섬유소 분해능 및 체외효소 활성)

  • 백근식;최지혁;성치남
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.130-135
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    • 2000
  • Decomposition rate of organic matiter in the mud flat of Sunchon Bay was estimated. Physicochemical parameters, cellulose degradation rate. distribution of heterotrophic bacteria, and extracellular enzymatic activities were measured from August 1997 to July 1998. Soil temperatures, water contents, concentration of $PO_4$-P and organic matter were -1-~$30^{\circ}C$, 42.1-53.1%, 0.0779-0.1961 mgig and 1.99-7.64%, respectively. Decomposition rate of cellulose film ranged from 7.7 to 100%imonth, high in summer and low in winter. The number of heterotrophic bacteria ranged from $0.87{\times}10^6 to 3.6{\times}10^7 $CUFsIg dq soil. Enzymatic activities of phosphatase, $\alpha$-D-gluEosidase, $\beta$-D-glucosidase and cellobiohydrolase, which were measured as decomposition rate of methylumbelliferyl(MLiF)-substrate, were 152.23-1779.80 nMIhr, 2.67-202.18 nM/hr, 5.03-258.26 M h r and 3.42-63.07 nM/hr, respectively Cellulose degradaaon rate and extracellular extracellular enzymatic activities were conelated with each other, and showed high correlation coefticiency with soil temperature.

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Changes of Chemical Compounds in Compost of Municipal Refuse;1. Changes of Carbon Compounds (폐기물의 퇴비화 과정중 물질 변화;1. 탄소화합물 변화)

  • Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.7 no.2
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    • pp.136-145
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    • 1988
  • To investigate the seasonal changes of various organic and inorganic compounds in compost, carbon compounds in compost were analyzed at various composting periods. Contents of organic matter, cellulose, total carbon, organic carbon and biodegradable carbon in compost were decreased with the progress of composting. In contrast, contents of lignin and nonbiodegradable carbon were increased a little with the progress of composting, but effective contents of lignin were decreased with the lapse of composting time, while effective contents of nonbiodegradable carbon were not changed. Total carbon contents in organic matter in compost were decreased within 9 weeks after composting, and then increased thereafter. Difference between average values of total and biodegradable carbon contents was 6.2%. Actual decay rates of all the carbon compounds were higher than decay rates of the compounds at all the experimental periods. Both of actual decay rate and decay rate of all the carbon compounds were increased rapidly within 2 weeks after composting, and thereafter the rates were increased slightly with the lapse of composting time. Especially the decay rates of cellulose were increased from 9 to 21 weeks after composting. Actual degradation capacity showed the same tendency to degradation capacity of all the carbon compounds in compost. Decay rate and degradation capacity of lignin in compost had minus values, while actual decay rate and actual degradation capacity had plus values. Highly positive correlations were observed among organic matter, cellulose, total carbon and biodegradable carbon one another. Nonbiodegradable carbon showed highly negative correlation with organic matter, cellulose, total carbon, organic carbon and biodegradable carbon, respectively. The same tendencies were observed between lignin and organic matter, cellulose, total carbon, organic carbon and biodegradable carbon. Highly positive correlation was observed between lignin and nonbiodegradable carbon in compost.

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Degradation of eriochrome black T by potassium ferrate (VI) (칼륨 페레이트에 의한 Eriochrome Black T 분해 연구)

  • Hoang, Nguyen Minh;Kim, Il-Kyu
    • Journal of Korean Society of Water and Wastewater
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    • v.36 no.3
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    • pp.167-175
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    • 2022
  • The degradation of EBT (Eriochrome Black T) in an aqueous solution was investigated at various values of pH, Ferrate (VI) dosage, initial concentration, aqueous solution temperature. The maximum degradation efficiency was 95.42% at pH 7 and in that experimental condition, the kapp value was 872.87 M-1s-1. The degradation efficiency was proportional to the dosage of Ferrate (VI). Also, the initial rate constant of EBT degradation increased with decreasing of the EBT initial concentration. In addition, the degradation rate of EBT was increased from 74.04% to 95.42% when the temperature in the aqueous solution was increased from 10℃ to 45℃. The activation energy value was 11.9 kJ/mol for EBT degradation. Overall, the results of the degradation experiment showed that Ferrate (VI) could effectively oxidize the EBT in the aqueous phase.

Effect of Cacao Extract on Hydrolysis of Oil vs. Emulsion in pH-Stat Digestion Model (pH-Stat Digestion Model에서 카카오 추출물이 Oil과 Emulsion의 가수분해에 미치는 영향)

  • Lee, Ji-Hyun;Shin, Jung-Ah;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.4
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    • pp.533-541
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    • 2016
  • Effect of polyphenols-rich cacao extract (CE) on lipid hydrolysis by pancreatic lipase was investigated by pH-stat digestion. Two types of substrate (oil vs. emulsion) prepared from soybean oil and CE were studied as types I and II. In the case of type I, addition of CE did not show retardation of lipid hydrolysis, showing that pancreatic lipase was not inhibited. Final digestibility rate (${\Phi}$ max, %) and initial rate (mM/s) of the 24-h aged control (52.31%, 0.03 mM/s) were similar to those of the CE-added sample (58.88%, 0.03 mM/s). However, in the case of typeII, the hydrolysis rates of the control and CE-added emulsion showed distinct differences as aging time increased to 43 days, showing lower digestion in the CE-added emulsion than the control. After 43 days, ${\Phi}$ max values of the control and CE-added emulsion were 92.13% and 77.68%, respectively.