• Proceedings of the KIEE Conference
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• 2003.07a
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• pp.564-566
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• 2003

전력 계통에서 안정한 전력을 공급하는 것은 매우 중요하다. 전력 계통의 오류는 전압 및 주파수를 감시함으로써 검출 가능하다. 본 논문에서는 디지털 위상 고정 루프를 이용한 전력 계통의 주파수 측정 장치를 제안하고 이를 구현한 결과를 제시하고자 한다. 제안한 주파수 측정 장치는 위상 고정 루프의 기본요소로 구성된다. 위상분별기는 배타적 논리연산을 통해 위상오차를 검출하고 위상의 앞섬 및 뒤짐의 검출이 가능하도록 설계하였으며, 전력 계통의 주파수 동특성을 고려해서 3차의 루프 필터를 설계하였다. DCO는 출력 주파수의 분해능을 고려하여 입력 신호를 정확하게 추정할 수 있도록 설계하였다. 제안한 주파수 측정 장치의 성능을 검증하기 위하여 모의실험을 통해 주파수 변동량의 측정 범위 및 정확도를 검토하였으며, FPGA와 CPU를 포함하는 하드웨어를 구현하였다. FPGA에는 Verilog HDL로 디지털 위상 고정 루프의 위상분별기와 DCO를 구현하였으며 루프필터는 소프트웨어로 구현하였다. 제안한 디지털 위상 고정 루프의 성능 검증을 위해 정밀한 함수 발생기의 출력을 인가한 후 출력 주파수를 측정한 결과 및 전력 계통에 대한 실험 결과를 제시하였다.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.9
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• pp.1299-1305
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• 2013

In this paper, we investigate a method of reducing the computational cost in speech recognition by dimensionality reduction of MFCC feature vectors. Eigendecomposition of the feature vectors renders linear transformation of the vectors in such a way that puts the vector components in order of variances. The first component has the largest variance and hence serves as the most important one in relevant pattern classification. Therefore, we might consider a method of reducing the computational cost and achieving no degradation of the recognition performance at the same time by dimensionality reduction through exclusion of the least-variance components. Experimental results show that the MFCC components might be reduced by about half without significant adverse effect on the recognition error rate.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2009.10a
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• pp.129-131
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• 2009

최근 추세에 따라 수치실험은 높은 해상도와 정확도를 요구하고 있다. 일반적으로 파랑장 계산은 광역모형을 도입하고 연구영역에 대한 지형상태에 대한 분해능을 가지는 격자에 대하여 별도의 상세역의 모형을 구성하게 된다. 여기서, 세역의 입사파는 광역의 결과를 토대로 추출하게 되는데, 이 때 결과의 연속성에서 문체를 가지게 된다. 대체로 이러한 문제점을 극복하기 위하여 가변격자체계와 스펙트럼 추출 둥의 방법을 사용하게 되지만 여전히 수치적 오류는 계속되고 있는 실정이다. 따라서, 본 연구에서는 최근 가장 일반적으로 사용되고 있는 SWAN모형의 Nested 모형에 대한 적용성을 확인하고자 한다. 이를 위하여 각기 다른 조석환경하에 있는 두 개의 항에 대한 실험을 실시하고 이에 대한 결과를 비교 분석하였다. 분석결과, Nested 모형으로 구성된 광역과 세역 경계에서의 추출된 값이 거의 동일한 값을 가지며 각기 다른 조석환경하에서의 결과 또한 다르지 않다는 것을 확인하였다.

• The Transactions of the Korea Information Processing Society
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• v.4 no.10
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• pp.2453-2460
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• 1997

Among the method of developing software there are many object-oriented analysis(OOA) techniques, and the new ones are being introduced continuously. The present OOA techniques, however, have difficulty in the identification and the verification of the objects and tend to ignore high-level control aspects of the problem due to the initial partitioning of them on the basis of the data. As a result, it brings inaccurate understanding and faults in the software which is required by users. Therefore the purpose of this paper is to design the TOVERC to verify completeness and consistency through cross-reference between the state transition diagram and the methods of object model in order to reflect the requirements of users in the analysis of software considering this problem.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.10a
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• pp.721-724
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• 2014

Ultrasonic sensor is used widely for many applications because low cost, simple structure, and low restriction. There are many difficulties to recognize an object by use an ultrasonic sensor, because of low resolution, poor direction, and measurement error. To improve the these problem, we use the various kinds of sensor arrangement methods, large amount of sensor, and change the arrangement pattern of sensor. In this paper, to obtain the most basic parameters for pattern recognition such as distance, dimension of the object, an angle of the object, we get the improved results by use the intelligent calculation algorithm based on Neuro-Fuzzy. This method use the multifarious output voltage of ultrasonic sensor by simple electronic circuit.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.159-167
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• 2014

In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.

• Journal of Life Science
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• v.29 no.12
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• pp.1408-1414
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• 2019

Enzymes are widely used in industrial applications such as detergents, food, feed production, pharmaceuticals and medical applications and major contributors to clean industrial products and processes. To screen, identify, and characterize the enzymes the zymography techniques are routinely used. The zymography technique is a simple, sensitive, and quantifiable technique that is widely used to detect functional enzymes following electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels. The method is a versatile two-stage technique involving protein separation by electrophoresis followed by the detection of enzyme activity in polyacrylamide gels under non-reducing conditions. It is based on SDS-polyacrylamide gel (PAG) copolymerization with substrates, which are degraded by the hydrolytic enzymes restored in enzyme reaction buffer after the electrophoretic separation. Any kind of biological sample can be applied and analyzed on zymography, including culture supernatants of microbes, plants extracts, blood, tissue culture fluids, enzymes in foods extracts and metaproteome. The advantage of zymography is that it is possible to directly detect the protein with activity on the electrophoretic gel as well as confirm the activity at the nanogram level. Thus, this zymography technology can be applied in various fields. However, these advantages are rather disadvantageous and can often lead to experimental errors. In this review, the advantages, disadvantages, and problem solving of zymography technique are described.

• Korean Journal of Heritage: History & Science
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• v.40
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• pp.387-411
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• 2007

The analysis of ancient DNA (aDNA) has become increasingly considerable anthropological, archaeological, biological and public interest. Although this approach is complicated by the natural damage and exogenous contamination of a DNA, archaeologists and biologists have attempted to understand issues such as human evolutionary history, migration and social organization, funeral custom and disease, and even evolutionary phylogeny of extinct animals. Polymerase chain reaction(PCR) is powerful technique that analyzes DNA sequences from a little extract of an ancient specimen. However, deamination and fragmentation are common molecular damages of aDNA and cause enzymatic inhibition in PCR for DNA amplification. Besides, the deamination of a cytosine residue yielded an uracil residue in the ancient template, and results in the misincorporation of an adenine residue in PCR. This promotes a consistent substitution (cytosine thymine, guanine adenine) to original nucleotide sequences. Contamination with exogenous DNA is a major problem in aDNA analysis, and causes oversight as erroneous conclusion. This report represents serious problems that DNA modification and contamination are the main issues in result validation of aDNA analysis. Now, we introduce several criterions suggested to authenticate reliance of aDNA analysis by many researchers in this field.

• KDI Journal of Economic Policy
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• v.34 no.4
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• pp.117-156
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• 2012

This paper decomposes and estimates trend/cyclical components of some key macro variables-GDP, inflation, and interest rate, using a simple DSGE model along with flexible trend specification. The extracted cyclical components of output and interest rate are similar to HP-filtered counterparts, despite some differences in persistence and volatility, while inflation resembles that from BK filtering. This implies that the usual practice of applying a single filtering method to the data of interest may be problematic. When the baseline model is extended to incorporate consumption habit and price indexation, habit turns out to be important in explaining the persistence of business cycles. Comparison of several alternative models shows that the usual practice of estimation of DSGE model using filtered data leads to biased results. Finally, various sensitivity analyses illustrate that (1) allowing for correlation between structural cyclical shocks and trend shocks and (2) including irregular components (in inflation rate) may deliver interesting/important implication for gap estimates.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.1A
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• pp.8-18
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• 2011

Visible light communication (VLC) using optical sources which can be simultaneously utilized for illumination and communication is currently an attractive option for wireless personal area network. Improving the data rate in optical wireless communication system is challenging due to the limited bandwidth of the optical sources. In this paper, we design the singular value decomposition (SVD)-based multiplexing multi-input multi-output (MIMO) system to support two data streams in optical wireless channels. In order to improve the spectral efficiency, the rate adaptation using multi-level pulse amplitude modulation (PAM) is applied according to the channel condition and we propose the method to allocate the optical power, the offset and the size of modulation scheme theoretically under the constraints of the nonnegativity of the modulated signals, the aggregate optical power and the bit error rate (BER) requirement. The simulation results show that the proposed allocation method gives the better performance than the method to allocate the optical power equally for each data stream.