• Title/Summary/Keyword: 분리봉

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Antibacterial Activity of Lactobacillus casei Isolated from a Fermented Milk Against Pathogenic Enteric Bacteria (발효유(醱酵乳)에서 분리된 Lactobacillus casei의 병원성 장내세균에 대한 항균작용)

  • Choi, Chul-Soon;Chung, Jae-Bong;Chung, Sang-In;Yang, Yong-Tae
    • The Journal of the Korean Society for Microbiology
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    • v.19 no.1
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    • pp.41-48
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    • 1984
  • The present study was undertaken to elucidate the antibacterial spectrum of L. casei phage type $J_1$ strain isolated from a fermented milk product against pathogenic enteric bacteria. Growth inhibitory effects and minimum inhibitory concentration(MIC) of culture supernatants of L. casei grown in MRS broth were measured by both plate culture method and microplate broth dilution technique against Salmonella typhi, Salmonella typhimurium, Shigella flexneri, Shigella dysenteriae, enterpathogenic E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The results are summarized as follows: 1. The MRS broth culture of L. casei gave a similar extent of growth inhibitory effects against S. typhi, S. typhimurium, S. flexneri, S. dysenteriae, E. coli, K. pneumoniae and P. aeruginosa, respectively. 2. The inhibitory effects of L. casei culture were observed either in whole broth culture or in culture supernatant, but neither the bacterial suspension nor the neutralized culture supernatant showed such as antibacterial activities. 3. The MIC titres of the culture supernatants were ${\log_2}5$ to ${\log_2}6$, whereas those of the neutralized culture supernatant dropped markdely to ${\log_2}2$ to ${\log_2}3$. These results indicated that major portion of growth inhibitory effects of MRS broth culture of L. casei against enteric bacterial pathogens was possibly due to the acids produced, and minor portion to other antibacterial substances.

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Studies on Constituents of Higher Fungi of Korea(XXXIII) -Antitumor Components of Trametes sanguinea- (한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究)(제33보) -간버섯의 항암(抗癌) 성분(成分)-)

  • Hong, Wha-Bong;Chung, Kyeong-Soo;Woo, Myoung-Sik;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.10 no.4
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    • pp.147-154
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    • 1982
  • To find antitumor components with low toxicity from natural resources, antitumor test of the water extract of the carpophores of Trametes sanguinea (L. ex Fr.) Lloyd. was undertaken. The carpophores of this fungus were collected in Gyeong Gi Province and extracted with hot water. The extract was purified by dialyzing through Visking tube and a protein-bound polysaccharide fraction was obtained. The fraction was tested for antitumor activity against sarcoma 180 implanted in mice. The tumor inhibition ratio of the fraction against the tumor was 72.4% at the dose of 10mg/kg/day for the period of ten days. The tumor in one of the eight mice was completely regressed. The antitumor components were found to be a polysaccharide and a protein. The hydrolysis of the polysaccharide moiety yielded three monosaccharides, and from the hydrolysate of the protein moiety 13 amino acids were identified.

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Performance evaluation of Multicast in an Integrated Services Packet Network (종합 서비스 패킷망에서의 멀티캐스트의 성능 평가)

  • Lee, Wang-Bong;Kim, Young-Han
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.36S no.1
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    • pp.9-19
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    • 1999
  • An increase of the number of the real time applications and the Internet hosts make the Internet architecture changed. Current Internet architecture has some problems to process the real time traffics. To solve these problem, a new Internet architecture is proposed as the Integrated Service model. In the current Internet, as multicast protocols, the QoS multicast and the best-effort multicast have been studied in their separate network environments. But, the Integrated Service Packet Network is a heterogeneous network composed of the QoS delivery domain and the best-effort delivery domain. Thus, those separated multicast protocols have limitations in an ISPN. In this paper, we propose a multicast protocol for the ISPN with the QoS and the best-effort multicasting, and analyze the performance of this protocol. As a result, we find that the packet losses are same for hybrid multicast and best-effort multicast when the bandwidth is sufficient. But, if there exist some background traffics, the hybrid multicast has less packet loss than of the best-effort multicast.

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Fatty Acid Composition and Electrophoretic Patterns of Proteins of Korean Soybeans (우리나라 콩의 지방산 조성 및 단백질의 전기영동 패턴)

  • Kim, Jong-Goon;Kim, Sung-Kon;Lee, Joon-Shik
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.263-271
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    • 1988
  • Fatty acid composition and electrophoretic patterns of protein of Korean soybeans were studied, The protein content showed an inverse relation with the lipid content, The content of lipid types of total lipid was in the order of neutral lipid, glycolipid and phospholipid, Cultivar 102-B had higher content of neutral lipid but lower content of phospholipid compared with others. The main component of neutral lipid was triglyceride followed by sterol. The main fatty acids of total lipid were linoleic, oleic and palmitic acids, which comprised over 85% of total fatty acids. The main fatty acid of neutral lipid, glycolipid and phospholipid were linoleic acid. The polyacrylamide gel electrophoretic pattern of protein showed seven bands. The cultivar 102-B had the highest content of IIS globulin. Sodium-dodecyl sulfate PAG electrophoresis demonstrated that all soybeans had similar composition of subunit.

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Determination of Plutonium in HANARO Irradiated Fuel by IDMS (동위원소희석 질량분석법에 의한 조사 후 하나로 핵연료 중 Pu 정량)

  • Jeon, Young Shin;Son, Sae Chul;Kim, Jung Suk
    • Analytical Science and Technology
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    • v.16 no.3
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    • pp.191-197
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    • 2003
  • Two methods, IDMS (Isotope Dilution Mass Spectrometry) and controlled potential coulometry are compared by the determination of Pu for the NBL, CRM No.122, $PuO_2$. The recovery of Pu was found to be $1.002176{\pm}0.000452$ within the relative standard deviation of 0.045% (95% conf. level) although a small size of sample ($0.9{\mu}g$-Pu) was used in IDMS. The recovery using controlled potential coulometry were obtained in the range of 0.9923~0.9960. The analytical results of IDMS and controlled potential coulometry were good agreement within 0.6~1%. Base on these experiment results, The plutonium in HANARO irradiated fuel rod that separated portion of top, middle, and bottom were determined. The measured values of Pu are 1.155 mg, 2.483 mg and 1.920 mg in one gram of sample(fuel+clad), respectively.

Evaluation of Fuel Cladding Failures from the Fission Product Activities in the Reactor Coolant (원자로 냉가수내의 핵분열생성물 방사에 의한 핵연료피복관 파손 평가)

  • Ho Ju Moon;Sung Ki Chae
    • Nuclear Engineering and Technology
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    • v.16 no.3
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    • pp.169-179
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    • 1984
  • An efficient procedure of evaluating the fuel cladding failures occurring in the normal operations of typical PWR's has been investigated through the analysis of fission product(FP) activities in the reactor coolant using an analytical model, FIPREL code. Performed by this code is an extensive study on the sensivities of FP activities to such physical parameters as enrichment, turnup, and operation temperature of failed fuel rod as well as the effective failure size quantified in terms of the magnitude of gap release coefficient. The results of study are generally in agreement with those by PROFIP method. In the presence of tramp uranium the portion of activities released from failed rod is separated by an iterative calculation based on the activity ratios of fission nuclides chemically more stable than iodines. Obtained are the linear power density and the number of failed rods, the effective failure size, and the mass of tramp uranium. The operation experiences of 4 cycles of Kori Unit 1 are analyzed and the results show that the model is highly reliable for the survey and evaluation of fuel rod conditions during reactor operations.

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Assessment of Controlled Low Strength Material using Pond Ash for Pipe Backfill Materials (매립석탄재 활용 CLSM의 관 뒤채움재 적용성 평가)

  • Young-Wook Kim;Young-Cheol Lim;Doo-Bong Yeon
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.11 no.1
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    • pp.32-38
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    • 2023
  • In this study, as part of the practical development of technology for CLSM using pond ash, the characteristics such as flowability, bleeding rate, and strength of the CLSM according to physical properties such as particle size distribution and particulate content of the pond ash were reviewed. As a result of analyzing the properties of the collected pond ash, it was found that the characteristics of density and particle size distribution were different. As a result of evaluating the characteristics of the CLSM for three types of pond ash, it was found that the blending conditions to satisfy the quality stipulated in ACI 229R were different, and mainly affected the particle size distribution characteristics and particulate content of the pond ash. In case of coarse-grained pond ash (PA-3), mixing conditions that satisfy the performance requirements stipulated in ACI 229R were not derived. But it is considered that further review is necessary according to particle size adjustment.

Estimation of Dynamic Displacements of a Bridge using FBG Sensors (FBG센서를 이용한 교량의 동적변위 추정)

  • Shin, Soobong;Yun, Byeong-Goo;Kim, Jae-Cheon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.13 no.3 s.55
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    • pp.101-109
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    • 2009
  • An algorithm is proposed for estimating dynamic displacements of a bridge by using FBG sensors and by superposing some measurable low modes. Modal displacements are obtained from the beam theory and the generalized coordinates are deduced from the strains measured by FBG sensors. By considering flexural and torsional modes occurred in bridges only as flexural modes of a simply supported beam by separating a bridge into multiple girders or parts, the proposed algorithm can be applied to various types of bridges. Guidelines are provided theoretically for determining the number of modes and the number of strain gages to be used. The proposed algorithm has been examined through simulation studies on various types of bridges, laboratory experiments on a model bridge, and field tests on a simple span PC Box girder bridge. Through the simulation study, the effects of the error in the vibration modes and measurement noise on estimating the dynamic displacements are analyzed.

Probiotic Effects of Lactobacillus plantarum and Leuconostoc mesenteroides Isolated from Kimchi (김치에서 분리한 Lactobacillus plantarum과 Leuconostoc mesenteroides의 프로바이오틱 효과)

  • Lee, Kyung-Hee;Bong, Yeon-Ju;Lee, Hyun Ah;Kim, Hee-Young;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.1
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    • pp.12-19
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    • 2016
  • The probiotic effects of kimchi lactic acid bacteria (LAB), Lactobacillus plantarum (Lab. plantarum) and Leuconostoc mesenteroides (Leu. mesenteroides), were studied. Lab. plantarum KCCM 11352P (LPpnu) and Leu. mesenteroides KCCM 11353P (LMpnu) were isolated from kimchi and were the predominant LAB. We compared their probiotic effects with Lactobacillus rhamnosus GG (LRgg), a well-known probiotic LAB. LPpnu showed better probiotic activities than LRgg. LMpnu also exhibited almost equal activities as LRgg. These two kimchi LAB strains exhibited resistance to gastric and bile acid, adhesion to intestines, and thermal stability. In particular, LPpnu showed excellent probiotic properties. In addition, LPpnu showed greater antioxidant activity by scavenging DPPH radicals or hydroxyl radicals than LMpnu or LRgg. LPpnu also inhibited growth of HT-29 human colon cancer cells by inducing apoptosis, increasing Bax and suppressing Bcl-2 expression compared to LMpnu or LRgg. Taken together, LPpnu and LMpnu could be used as probiotics, and LPpnu exhibited the most beneficial probiotic activities with anti-oxidant and anti-cancer properties.

Effect of Adding Freeze-Dried Kimchi Powder on Flavor and Taste of Kimchi Snacks (동결김치분말을 첨가한 스낵류의 맛과 품질에 미치는 영향)

  • Cho, Yong-Bum;Park, Woo-Po;Hur, Min-Su;Lee, Yang-Bong
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.919-923
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    • 2004
  • Effects of adding 0, 2, 4, 6, and 8% freeze-dried kimchi powder (FDKP) on kimchi snack quality were examined. Headspace volatile compounds of 4% FDKP snack, receiving highest overall preference in sensory evaluation results, were analyzed, and 26 compounds were identified, including 13 aldehydes, 3 sulfides, a ketone, an acid, a terpene, and 7 other compounds. Pearson correlation analyses were carried out to determine a correlation between the concentration of FDKP and the amount of each volatile compound. Acetaldehyde, propanal, dimethyl sulfide, dimethyl disulfide, acetic acid, and d-limonene showed correlation coefficients above 0.90; selective ion move (SIM) analysis also showed above 0.97 for highly correlated compounds. Results suggest that these compounds can be used as indicators fur kimchi flavor of FDKP snack.