• Title/Summary/Keyword: 분리공시

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Polymorphism and Genetic Relationships Among Magnaporthe grisea Isolates Obtained from Various Hosts by Using Repetitive DNA Sequences (기주가 다른 Magnaporthe grisea 균주간의 Polymorphism과 유전적 유연관계 분석)

  • 김홍기;김영태
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.389-394
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    • 1996
  • 도열병균, Magnaporthe grisea, 균주간의 유전적 유연관계를 분석하고 그들의 유전에 관한 '기본 정보를 얻고자 DNA polymorphism 분석을 실시하였다. 기주가 다른 도열병 균주들이 공시되었고 cloning에 의해 벼 도열병균 KJ201레이스 균주로부터 생성된 임의 선발 genomic clone들이 공시균주들간의 polymorphism을 밝히기 위해 사용되었던 바 그중 repectitive sequence를 보유한 repeated copy clone 하나가 선발되었다. Clone pMJ6에 의해 밝혀진 repetitive sequence는 Southern hybridization시 벼 분리균주에는 약 30개, 다른 기주 분리균에도 20∼33개의 밴드를 형성하였다. 반면 피 분리균주에는 단지 두 개의 밴드만을 나타내 분리기주가 다른 균주간에 뚜렷한 polymorphism이 존재하였으며 parsimony 분석에서도 역시 아주 먼 cluster를 형성하여 피 분리균은 다른 기주 분리균과 유전적으로 상당히 먼 것으로 추정되었다. 공시균의 genomic DNA를 HindIII로 처리했을 때 pMJ6에 의한 밴드양상은 공시균을 EcoRI으로 처리했을 때의 MGR probe의 밴드 양상과 유사하여 이 repeated copy clone이 도열병균주간의 유전적 유연관계를 분석하는데 MGR 못지않게 유용할 것으로 보인다.

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Exploring Alternative Real Estate Assessment Systems in Korea (부동산가격공시제도의 문제점과 개선방안에 관한 연구)

  • Koo Dong-Hoe
    • Journal of the Korean Geographical Society
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    • v.41 no.3 s.114
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    • pp.267-282
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    • 2006
  • Korea officially appraises and publicizes three different values of the land and buildings for the same lot. The values are assessed by the Ministry of Construction and Transportation, the Ministry of Government Administration and Home Affairs, and National Tax Service. A fundamental problem of this appraisal system is that the values of land and buildings are separately assessed, even though they are bought and sold as a single entity in the real estate market. In order to solve this problem, an alternative real estate assessment system should be developed by the central government.

The Hardening Characteristics of Underwater Concrete According to the Cellulose Underwater Non-segregation Admixture and Its Preparation (Cellulose계 수중재료분리 방지제의 제조 및 첨가에 따른 수중콘크리트의 경화특성)

  • Shin, Dong-Gil;Song, Yon-Ho;Lee, Chul-Tae
    • Applied Chemistry for Engineering
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    • v.16 no.2
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    • pp.267-274
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    • 2005
  • Preparation of cellulose type underwater non-segregation admixture was attempted and the hardening characteristics of underwater concrete according to the addition of this admixture was investigated in order to make underwater concrete with the compressive strength ratio of 0.8 to that of concrete manufactured in common atmosphere. The proposed underwater non-segregation admixture consisted of methyl cellulose of 0.4% by weight, silicon type antifoaming agent of 20% by weight, and sodium aluminate of 0.1% by weight to the amount of cement as setting accelerant, respectively. As the proposed non-segregation admixture was increased, the amount of suspended solid decreased, air content in concrete was increased but the flow loses by elapsed time did not change. The proper amount added of the proposed non-segregation adimixture was 0.8 wt% to the amount of cement. The compressive strength of the test sample underwater concrete manufactured by the addition of the proposed admixture was $325Kg/cm^3$, and the ratio of compressive strength of this sample concrete to that of a concrete manufactured in air was 0.94.

Utilization the Tofu-Residue for Production of the Bacteriocin 1. Cultural Conditions of Bacillus sp. for Amylase (박테리오신의 생산을 위한 두부비지의 이용 1. 두부비지에서 분리한 Bacillus sp.에 의한 Amylase의 생산조건)

  • 이선희;이명숙
    • Journal of Food Hygiene and Safety
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    • v.15 no.3
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    • pp.271-276
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    • 2000
  • A amylase producing bacteria were isolated from tofu residue and identified as Bacillus sp. according to the morphological and biochemical properties, which were named Bacillus sp. GM7330 and Bacillus sp. GM7312. The cultural condition for the production of amylase was showed on 5% tofu residue added 3% glucose and 0.15% yeast extract. And incubated during 72 hrs at 30。C, Bacillus sp. GM7330 and Bacillus sp. GM7312 were producing amylase of 488 units and 341 units.

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Occurrence of Fusarium Wilt on Cruciferous Vegetable Crops and Pathogenic Differentiation of the Causal Fungus (십자화과 채소작물에서의 후사리움 시들음병 발생과 그 원인균의 병원성 분화)

  • 문윤기;김완규;조원대;성재모
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.93-101
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    • 2001
  • Occurrence of Fusarium wilt was surveyed in fields of cruciferous vegetable crops in Korea from 1996 to 1998. The disease severely occurred up to 40% in fields of Chinese cabbage and radish but slightly in Fields of cabbage. A total of 123 isolates was obtained from roots of the diseased plants and identified as Fusarium oxysporum based on the morphological and cultural characteristics. Pathogenicity of nine isolates selected from the isolates was tested by artificial inoculation to the hosts. All the isolates had similar virulence on Chinese cabbage and cabbage, although there were some differences in virulence on cultivars tested among the isolates. The isolates from radish were more virulent to radish than those from Chinese cabbage and cabbage. All isolates from the crucifers were not virulent to eight species of vegetable crops except the crucifers. The results of pathogenicity tests showed that the pathotype of Chinese cabbage-infecting isolates was identical to that of cabbage-infecting isolates, but somewhat different from that of radish-infecting isolates.

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Determination of Optimal Mixture Proportion of Segregation Reducing Type Superplasticizer for High Fluidity Concrete (고유동 콘크리트용 분리저감형 유동화제의 최적배합비 결정)

  • 한천구;김성수;손성운
    • Journal of the Korea Concrete Institute
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    • v.14 no.3
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    • pp.275-282
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    • 2002
  • High fluidity concrete needs high dosage of superplasticizer to acquire sufficient fluidity and high contents of fine powder and viscosity agents to prevent segregation. But it requires high manufacturing cost and has difficult in quality control. Therefore, in this paper, determination of optimal mixture proportion of segregation type superplasticizer for high fluidity concrete and manufacturing high fluidity concrete by applying developed segregation reducing type superplasticizer are discussed using flowing concrete method. According to test results, as dosage of superplasticizer increases, it shows that fluidity and bleeding increase, while air contents and ratio of segregation resistance decrease. It also shows that adding viscosity agent into it reduce bleeding and improve segregation resistance. Dosage of AE agent into it containing viscosity agent recovers loss of air contents during flowing procedure. Combination of proper contents of superplasticizer, viscosity agent and AE agent make possible to develope segregation reducing type superplasticizer Compressive strength of high fluidity concrete applying flowing method with it is higher than that of base concrete. No differences of compressive strength between compacting methods are found.

Isolation and Characterization of Plant Growth Promoting Rhizobacterium Bacillus subtilis YK-5 from Soil (토양에서 식물생육촉진 활성을 가진 균주 Bacillus subtilis YK-5의 분리 및 특성)

  • Yeo, Soo-Hwan;Yook, Young-Min;Kim, Hyun-Soo
    • KSBB Journal
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    • v.24 no.4
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    • pp.334-340
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    • 2009
  • For the isolation of a plant growth-promoting rhizobacteria, strain YK-5 was selected from approximately 400 thermostable strains isolated from special soil samples. Strain YK-5 produced an antifungal compound, and optimum carbon and nitrogen sources for the production of the antifungal compound were investigated against Aspergillus flavus as a test strain. Modified LB medium containing 1% peptone, 1% yeast extract and 5% black sugar was determined to be the optimal medium for growth and antifungal compound production. Culture broth of strain YK-5 potently inhibited growth of the phytopathogenic fungus Fusarium oxysporum KACC 40052 for 7 days. The plant growth-promotion function of strain YK-5 was tested against radish and rice in pot trials. Leaf number, plant height and root length in YK-5-treated radish markedly exceeded (> 60%) those of untreated radish. Leaf length and white rootlet development were markedly more prominent than in commercially-treated rice plants. Strain YK-5 was determined to be Bacillus subtilis YK-5 by physiological, chemotaxonomical, and phylogenetical analyses.

Studies on the evaluation method of structural concrete strength using joint separation test body (접합분리 시험체를 사용한 구조체 콘크리트 강도 평가에 관한 실험적 연구)

  • Kim, Seong-Deok;Lee, Seon-Ho;Kim, Kwang-Ki;Jung, Kwang-Sik;Lim, Nam-Ki;Jung, Sang-Jin
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.993-996
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    • 2008
  • It has been reported that destruction test by core collection is the most reliable of the structural concrete strength in present building construction field. But it causes low efficiency by damage and cutting in structure due to the core collection. It also has some problems in repairing. Additionally in case of strength test with management specimen, different environment compared to the structure environment cause problems about estimation precise structure strength. Therefore, it is required to develop structure direct strength test that has test values and credibility above the ones obtained by core specimen collection strength test and seasonal specimen test to suggest a reasonable and practical management method of structural concrete.

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Nutritional and Cultural characterizations of microorganism capable of producing antagonistic activity against Streptococcus mutans (S. mutans에 항균력(抗菌力)을 나타내는 균주(菌株)의 배양학적(培養學的) 성질(性質))

  • Park, Myung-Ho
    • Journal of Technologic Dentistry
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    • v.21 no.1
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    • pp.139-144
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    • 1999
  • The optimum culture conditions for an antibiotics from Actinomyces sp. were investigated. The optimum composition of medium for antibiotics production was 1% glucose, 1% soybean meal, 0.5% NaCl, 0.1% $CaCO_2$, and the optimum initial pH was 7.0. And the antibiotics showed highest activity when the strain isolated from soil was aerobically cultivated at $28^{\circ}C$ for 72hours under the optimum conditions. A production of the antibiotics from Actinomyces sp. begins at the 36th hours and then reached the maximum at the stationary phase developed at the 72th hours under the optimum conditions.

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An Analysis of Public Noticed Land Prices Using GIS (GIS를 이용한 공시지가 분석)

  • Kang, In-Joon;Song, Seok-Jin;Kang, Ho-Yun
    • Journal of Korean Society for Geospatial Information Science
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    • v.17 no.4
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    • pp.121-125
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    • 2009
  • The Public Noticed Land Price system was developed in order to establish a practical and consistent land price information system, where land prices are appraised and publicly noticed according to the Law Governing the Public Notice of Real Estate Prices and Appraisals.The appraisal process for evaluating and appraising the public noticed land price in conducted in phases, where the nation's land is divided into human and spatial portions according to administrative districts. Depending on the subjective judgement of the appraiser, it is therefore possible for discrepancies to occur in land prices for lots near the boundaries of administrative districts. There is the computerized support system to maintain the public noticed land prices balance between the boundaries of cities, counties, and districts (the units in which evaluation and appraisal are conducted to determine public noticed land prices). But, due to that system is divided into attribute and spatial data information, it is possible for discrepancies to occur in land prices for lots near the boundaries of administrative districts. The purpose of this study to suggest the reasonable methods on discrepancies in public noticed land prices through spatial analysis using GIS.

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