• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.10a
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• pp.113-114
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• 1999

일차 오존 표준 분광기(SRP)를 제작하여 오존 측정의 정확성을 확보하고, 이것을 국가 표준으로 이용하여 현장 분석기를 차례로 교정하는 체계(그림 1 참조)를 구축하기 위하여, 먼저 자외선 흡광 광도법을 근간으로 하여 오존 SRP 후보 기기를 제작하였다.(중략)

• YAKHAK HOEJI
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• v.30 no.4
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• pp.185-192
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• 1986

Authors developed the computer application program (language: APPLE SOFT BASIC) for derivative spectrophotometry. By means of this program, derivative of spectral absorbance with respect to wavelength is recorded versus wavelength. To try this program in connection with spectrophotometer system, the authors have done the simultaneous quantitation of pyridoxine center dot HCl and nicotinamide in the mixture, and the result was compared with that of absorbance method.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.11a
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• pp.243-244
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• 2003

오존은 매우 불안정한 물질로서, 기타의 환경 대기 가스 분석법에서와 같이 해당 성분의 측정기를 표준 가스로 교정한 후 분석할 수 없다. 이러한 이유로, 환경 대기 중의 오존을 분석하는 일반 분석기의 교정을 목적으로, 본 연구실에서는, 국가 일차 오존 표준분광기(O$_3$-SRP)를 제작하여 운영하고 있다. 운영되고 있는 $O_3$-SRP의 측정 원리는 자외선 흡광 광도법이며, 농도값의 결정은 순수하게 Beer-Lambert 법칙에 따른다. Beer-Lambert 법칙에 따라서 오존 농도를 절대적으로 측정하기 위해서는 몰 흡광계수, 광로의 길이, 투과도, 온도 및 압력을 정확히 측정하여야 한다. (중략)

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.25 no.4
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• pp.81-96
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• 2020

Berthlot's reaction spectrophotometric method is generally used for the analysis of dissolved ammonium in seawater, but in recent years, a fluorescence method using an orthophthaldialdehyde-sulfite (OPA) fluorescent reagent is actively used internationally. In this study, we investigated the effects of the detection limit between the analysis methods, the reagent refractive index inherent in the spectrophotometric method, and the use of different calibration curves to understand the cause of the difference in dissolved ammonium concentration (about 0.31 𝜇M) observed in the seawater samples and a nutrient reference material between two institutions (KIOST (spectrophotometric method, one-order linear regression gradient only), Australia CSIRO (fluorescence method, quadratic formula)) conducted onboard the Australian R/V Investigator in 2017. The method detection limit (0.063 𝜇M) and the reagent refractive index background value (0.054 𝜇M) of the spectrophotometric method measured in this study could explain the difference in dissolved ammonium concentration values of the two institutes about 20% and 17%, respectively. However, when the concentration of the calibration curve of the spectrophotometric method was calculated using the same quadratic as the fluorescence method or the slope and intercept of linear regression, the difference in the dissolved ammonium concentration between the two institutions was reduced to less than the detection limit of the spectrophotometric method. Therefore, the difference in the concentration of dissolved ammonium between the two institutions, found in the nutrient reference materials and the seawater field sample during the international onboard nutrient inter-comparison experiment, may be attributed to be the effect of the different calibration curves used in the two methods rather than the effect of the difference in two analytical methods. When comparing the dissolved ammonium data from seawater samples in the future, it is recommended to pay attention to the information on the baseline, number of standard solutions, and calibration curve used in the analysis.

• Analytical Science and Technology
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• v.20 no.5
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• pp.406-412
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• 2007

The formation of inclusion complexes between ${\beta}$-cyclodextrin and diethylenetriamine substituted-pyridine copper(II) perchlorate; [Cu(dien)(sub-py)] $(ClO_4)_2$, were studied by spectrophotometric methods. On account of charge-transfer band(MLCT) and $^2T_2{\rightarrow}^2E$, the two high peaks were observed as an inclusion complex for the [${\beta}$-CD]$[Cu(dien)(p-Cl-py)]^{2+}$ in the ultraviolet region of the spectrum. The ${\beta}$-CD and $[Cu(dien)(sub-py)]^{2+}$ ion formed a 1:1 complex, and the formation constants were decreased with the increasing temperatures, due to weak binding energy between ${\beta}$-CD and $[Cu(dien)(sub-py)]^{2+}$ ion. This reaction was controlled by enthalpy. In a correlation of the Hammett substituent constants and formation constants for the reaction, formation constants were increased by strong binding energy in the inclusion complexes when electron donating groups were substituted in pyridine ring.

• Analytical Science and Technology
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• v.16 no.2
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• pp.152-158
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• 2003

This study was conducted to evaluate two sample digestion procedures and instrumental determination parameters for analysis of lead in bone. Amputated human legs were treated by acid digestion or microwave dissolution prior to spectrometric analysis. Inductively coupled plasma mass spectrometry (ICP-MS) and graphite furnace atomic absorption spectrometry (GF-AAS) were used for determining bone lead levels. Recovery efficiencies using standard reference material from acid digestion measured by ICP-MS were in good agreement with those of the certified value, but in cases of acid digestion by GF-AAS and microwave digestion by both two methods, recovery underestimated and overestimated, respectively. For the bone samples, the lead concentrations obtained by ICP-MS after acid digestionwere in good agreement with those by GF-AAS (correlation coefficient = 0.983), but GF-AAS gave systematically higher values than ICP-MS. While a good agreement between two analytical methods after microwave digestion was also obtained (correlation coefficient = 0.950), bone lead concentrations from microwave were relatively higher than those from acid digestion. In conclusion, the use of the simple nitric acid digestion procedure at an ambient temperature coupled to ICP-MS seems to be efficient for the determination of lead in bone in consideration for both the convenience and validity.

• Bulletin of the Korean Space Science Society
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• 2011.04a
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• pp.21.1-21.1
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• 2011

우리는 분리형 쌍성 11개(원궤도 7개, 타원궤도 4개), 반분리형 쌍성 7개와 접촉형 쌍성 9개(근첩촉형 3개, 접촉형 2개, 초과 접촉형 4개)를 포함하는 27개의 다양한 종류의 표준식쌍성의 분석하여 절대량을 구하였다. 표준 식쌍성은 식쌍성의 광도곡선이 적어도 두 파장 영역 이상에서 그리고 모든 위상에서 골고루 관측되었고, 쌍성의 두 구성원 별에 대하여 시선속도 곡선이 발표된 식쌍성, 즉, 이중분광선 식쌍성 (Double line spectroscopic eclipsing binariy)으로 정의한다. 우리는 표준식쌍성의 370개의 후보에 대하여 문헌을 조사하여 관측 데이터를 확보 하였다. 이중에서 시선속도곡선과 광도곡선 자료가 동시에 존재하는 식쌍성은 80개이다. 이번 연구에서 우리는 표준식쌍성의 관측 질을 고려하여 27개의 식쌍성을 선택하여 절대량을 계산하였고, 이전 연구자들이 발표한 절대량과 비교 하였다. 이 논문에서 제시한 표준식쌍성의 유용성을 확인하기 위해 소마젤란은하에서 발견한 식쌍성의 광도곡선을 분석하여 측광학적인 해를 구하는 작업을 수행하였다. 이를 위하여 표준 식쌍성을 식쌍성의 종류별로 구분하고, 소마젤란은하에서 발견된 식쌍성 20여개를 선정하였다. 선정된 식쌍성을 표준식쌍성의 광도곡선과 형태를 비교하여 초기값을 결정하였고, 반복적인 차등보정법으로 측광학적인 해를 구하였다. 이 논문에서 제시한 표준식쌍성이 소마젤란은하에서 발견된 식쌍성들의 광도곡선의 형태를 대부분 포함하는 다양한 분포로 형성되어 있고, 이에 따른 측광학적인 해가 관측과 잘 일치함을 보여주고 있다.

• Journal of Astronomy and Space Sciences
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• v.14 no.2
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• pp.233-241
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• 1997

New UBV Photometric observations for a close binary RZ Cas were obtained at the Chungbuk National University Observatory for 42 nights from October 1991 to March 1993, and standardized UBV light curves were constructed. From the primary light curves, the eight minimum times were determined by using a new light element. We confirmed the primary minimum was totality and derived the average duration was 18.1 minutes corresponding to $0.^{p}01$. The photometric and spectroscopic solutions were calculated with the obtained light curves and the radial velocity curves of Duerbeck & Hanel(1979) by using Wilson-Devinney method. From these values, the absolute dimensions of this binary system were estimated.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.55.1-55.1
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• 2010

대마젤란 은하에 속한 식쌍성의 시선속도 곡선을 완성하기 위하여 2009년 11월 칠레에 위치한 Cerro Tololo Inter-American Observatory (CTIO)의 1.5m 망원경과 R-C Spectrograph를 사용하여 중 분산 분광관측을 수행하였다. 분광관측은 380nm - 540nm 영역에서 $H_8,\;H_{\varepsilon},\;H_{\delta},\;H_{\Gamma},\;H_{\beta}$ 등의 흡수선을 관측하였다. 중분산으로 관측된 식쌍성의 각 구성원별에서 나오는 흡수선들은 구별되지 않으므로 이를 구별하기 위하여 구성원들의 Flux 비와 함께 gauss curves fitting 방법으로 각 흡수선들을 2개의 성분으로 분해하여 시선속도를 측정하고, 시선속도곡선을 완성하였다. 완성된 이중 시선속도 곡선은 Wilson & Devinney 차등 보정법으로 분석하여, 궤도 장반경, 질량비, 계의 시선속도 등을 구하였다. 또한 각 식쌍성들의 광도곡선을 분석하여 측광학적인 해를 구하고, 최종적으로 분광 및 측광관측의 해를 혼합하여 대마젤란은하 식쌍성들의 절대량을 구하였다. 이후 이를 바탕으로 거리를 구하여 기존에 발표된 마젤란은하의 거리와 비교하였다.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.382-389
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• 2017

Currently, reticulocyte experimental calculation technology used in clinical laboratories are divided two types: manual and automated. Manual reticulocyte counting using a microscopy lacks accuracy due particularly to its low reproducibility, affecting the accuracy of manual reticulocyte count. Moreover, Automatic blood corpuscle analyzer flow cytometry is difficult to be used in underdeveloped countries and small scale laboratories due to relatively high cost. Therefore, this study tried to find a new method to complement these drawbacks. The aim of this study was to compare the stained reticulocytes count by spectrophotometer and also to analyze the statistics of spectrophotometer and flow cytometer. The same 8 EDTA samples were repeated 36 times to compare the agreement between spectrophotometer and flow cytometer. This study measured the specimen diluted 600 times at 700~780 nm by 10 differences. Wavelength between 710 to 730 by absorbance showed a positive correlation between standard data and test data (r=0.967, p<0.01), presenting a correlation between variables. Statistical analyses of regression for test and standard parametric data, the optimal dilution factor was 600 times. Therefore, this study tried to technical utilizes such as contributing economical for the reticulocyte absorbance apply from the auto spectrophotometer, a monitoring system for the reticulocyte relation anemia, etc. Therefore, more extensive studies, including an auto chemical analyzer application, will be needed.