• Journal of the Korea Organic Resources Recycling Association
• /
• v.10 no.3
• /
• pp.74-84
• /
• 2002

This experiment was carried out to evaluate supplemental effects of various preservatives to preserve the Brewers grain and Soymilk from decay and degeneration. The NaCl, organic acid(Ca-propionate), microbial fermenter(H/MF inoculant), and methionine hydroxy analogue(MHA) were used as additives in order to compare preservability of brewers grain and soymilk by-product during 20 days at July to August. Most treatments were appeared rapid decay and degeneration as soften and covered with molds on surface from the early days of experiment. However, MHA treatments showed any change until 20 days of storage. In terms of pH and $NH_3-N$ contents, only MHA treatment showed a stable and low value. The other treatments including Control showed high values as time flowed or unstable states. In conclusion, when high moisture agricultural by-product was treated by over 5% of MHA, there was no significant physicochemical changes in long term preservation over 20 days at summer season.

• Horticultural Science & Technology
• /
• v.30 no.1
• /
• pp.42-49
• /
• 2012

This experiment was conducted to find out the effects of nitrous oxide ($N_2O$) on the postharvest quality of 'Janghowon hwangdo' peach fruits. Fruits were harvested at commercial maturity for marketing in late September, and treated with 70% $N_2O$ + 20% $O_2$ + 10% air, 80% $N_2O$ + 20% $O_2$, and 90% $N_2O$ + 10% $O_2$ for 48 h, and then stored at $15^{\circ}C$. No significant treatments for soluble sugar and titratable acidity contents were detected. However, good appearance and taste in peach fruit were maintained better in 80% $N_2O$ treatment than in air treatment. The treatment with 90% $N_2O$ had negative effects on weight loss and taste because of rotting by anaerobic fermentation. 80% $N_2O$ treated fruit had significantly higher fungus (Botrytis cinerea) growth inhibition of saprogenic approximately than air treatment until 12 days of storage. The browning and rotting at surface of peach were also retarded when peaches were treated with 80% $N_2O$ before they were artificially wounded. The activity of polyphenol oxidase (PPO) was inhibited about 80% in peach of 80% $N_2O$ treatment compared with in air treatment. The result showed that 80% $N_2O$ treatment was able to extend the shelf life of peach fruits through maintaining taste and inhibition of softening and browning by rotting and wounding during storage.

• Ecology and Resilient Infrastructure
• /
• v.6 no.4
• /
• pp.217-226
• /
• 2019

Optimum engineering methods for bank protection were classified based on steepness of bank slope and an existence of waterfront facility in the floodplain, and a new concept of eco-hybrid rolling mat method which could be applicable for the unfitted cases with previously developed countermeasures was suggested in this study. The eco-hybrid rolling mat method can be constructed while maintaining the river environment and ecosystem that does not interfere with the ground and slopes, when bank erosion occurs, it is an economical and efficient construction method that can protect the revetment and the bank slope immediately. The developed eco-hybrid rolling mat method was verified for the designed structure, system, function and effect based on large-scale river experiments including field exposure and decomposition test. As a result, the normal operation and effect of the rolling mat ted under low and high velocity conditions were confirmed with respect to bank protection. The effect of bank erosion prevention was quantitatively validated by sediment concentration monitoring and analysis, and the product specification of the eco-hybrid rolling mat was presented based on the standardized mat applied in real-scale tests.

• Korean Journal of Poultry Science
• /
• v.20 no.1
• /
• pp.33-41
• /
• 1993

Among the several factors that affect shelf-life of washed shell eggs, storage temperature and relative humidity were the most important. Besides those factors listed above, temperature of washing water, composition of foreign substances and washing method of eggs were also the factors affecting the shelf-life of the eggs. The effect of sanitizer treatment was significant in extending the shelf-life of eggs compared with washed and unwashed eggs. In case of oil coating treatment, the shell eggs treated showed the better results than that of washed and unwashed eggs because the coating materials prevented the moisture evaporation from the inner shell eggs and kept the contamination of microorganisms from the environment. Consequently, it is considered that reducing egg shell contamination of microorganisms and proper treatment could be the key to extend the shelf-life of shell eggs.

• The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
• /
• v.5 no.3
• /
• pp.78-87
• /
• 1994

생물학 및 의학등의 생명과학에서는 현미경을 이용하여 생체물질 즉, 생체조직을 관찰하고 이에 대한 조직의 검사 결과를 판정하고 발표한다. 이때 필히 고정과정(fixation process)을 거쳐야 한 다. 즉, 생체조직중 조직의 구조, 특정 세포나 바이러스 및 효소등을 관찰할 때 고정과정을 거쳐 조직을 절편하고 이를 염색하여 현미경으로 검사하게 된다. 고정과정이란 생체물질을 안정화시키고 자기분해 혹은 부패를 방지하여 보존이 가능하도록 변화시키 는 과정으로, 조직내의 용해성 물질을 불용성 물질로 변형시키는 과정이다. 고정과정을 거친 생체조직 은 구조를 보존하고 있기 때문에 조직의 훼손이 없는 상태에서 절편이 가능하고 또한 염색상태를 좋게 하며 관찰시 contrast를 증진시킨다. 만약 고정과정을 거치지 않으면 물질의 세포막이 파괴되고 단백질 등의 물질이 용해되어 조직의 변형을 일으켜 제대로 조직을 관찰할 수 없게 된다. 고정과정에는 크게 화학적 고정법과 물리적 고정법이 있다. 화학적 고정법은 생체조직을 화학용액에 처리하는 방법이며, 물리적 고정법은 직접적인 열 혹은 초음파등으로 물질을 고정시키는 방법이다. 표 면과 내부의 열전도가 달라져 고정이 균일하게 되지 않는 단점을 가지고 있기 때문에 보통 2~6일의 고 정시간을 요하는 화학적 고정법을 사용하고 있다. 따라서 조직에 대한 총 검사시간이 최소 6일에서 최 대 12일이 요구된다. 병원등에서 조직검사의 결과가 늦게 발표되는 사유는 바로 화학적 고정법을 사용 하여 생체조직을 관찰하고 그 결과를 판정하기 때문이다. 본 고에서는 마이크로파를 이용하여 약 3시간만에 조직의 상태를 관찰할 수 있는 고정법을 소개한다. 마이크로파를 이용하여 조직을 고정하는 고정방법을 기존의 고정법과 비교하여 이들의 장단점을 나타 낸다. 본 연구자에 의해 개발된 마이크로파 고정기를 소개하고, 이를 이용하여 생체물질을 고정한뒤 절 편, 염색하여 현미경 관찰결과를 발표하여 본 연구의 방법이 기존 방법보다 우수함을 나타낸다.

• Korean Journal of Microbiology
• /
• v.53 no.4
• /
• pp.344-346
• /
• 2017

Lactobacillus plantarum is widely found in fermented foods and has various phenotypic and genetic characteristics to adapt to the environment. Here we report the complete annotated genome sequence of the L. plantarum strain JBE245 (= KCCM43243) isolated for malolactic fermentation of apple juice. The genome comprises a single circular 3,262,611 bp chromosome with 2907 coding regions, 45 pseudogenes, and 91 RNA genes. The genome contains 4 malate dehydrogenase genes, 3 malate permease genes and various types of plantaricin-synthesizing genes. These genetic traits meet the selection criteria of the strains that should prevent the spoilage of apple juice during fermentation and efficiently convert malate to lactic acid.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.3
• /
• pp.167-171
• /
• 2004

The growth of soybean sprout was greatly influenced by watering systems： Fixed watering system (water tub was loaded at ceiling upper of culture box and water was showered by bottom holes) was estimated the better than that of reciprocating watering and tub immersing watering because it could cool down the temperature in culture box and wash the organic substances on the body of sprout. The fixed watering system showed good body color and preventing effect of partial rotting of sprout because it could discharge $\textrm{CO}_2$ gas effectively in culture box and keep the concentration below 5%. The concentration of gases at the bottom (about 30 cm height from basal plate) of culture box in fourth or fifth days was L6% for $\textrm{CO}_2$ and 13-16% for $\textrm{O}_2$, respectively. The optimum gas concentration in culture box was considered to be over 10% for $\textrm{O}_2$ and below 5% for $\textrm{CO}_2$.

• Korean Journal of Environmental Agriculture
• /
• v.25 no.3
• /
• pp.276-283
• /
• 2006

To evaluate the effectiveness of the foliar spray of calcium on "Baekdo" peach fruit, we carried out experiments in the orchards. The sprays were applied with $CaCl_2\;(Ca:\;400mg.kg^{-1})\;and\;CaCl_2$ with adjuvants (amino acid, $2g.kg^{-1}$; phytic acid, $2ml.kg^{-1}$ and wood vinegar, $2ml.kg^{-1}$) for four times from June 12 through July 4 at weekly intervals. The fruits and leaves were evaluated for Ca content, firmness and incidence of Brown rot caused by Monilinia fructicola at harvest To evaluate fruit quality included Ca content, firmness natural decay during the storage, the fruits were stored at room temperature for 14 days. The Ca content in leaf and fruit flesh at harvest was significantly increased (P=0.05) in $CaCl_2$ + amino acid treatment among $CaCl_2$ treatments. However, there was not significant Ca content in fruit peel. The firmness of flesh increased significantly (P=0.05) in $CaCl_2$ + amino acid treatment. The natural decay (Rhizopus stolonifers) during storage at the room temperature for 14 days, the fruit treated with $CaCl_2$ + wood vinegar exhibited lowest (P=0.05) incidence. Also, the firmness of the fruit during storage was firmer with treated $CaCl_2$ than untreated fruit. In the treatments of $CaCl_2$ + phytic acid and $CaCl_2$ + amino acid, it was possible to reduce incidence of Brown rot caused by M. fructicola most effectively in the field. In addition, inoculation with M. fructicola in fruits was also the most effective treatment for inhibiting disease development in vitro. These results suggested that the foliar spray of $CaCl_2$ with adjuvants increased the content of Ca and firmness of the fruits positively. It also inhibited the natural decay and the Brown rot effectively.

• Korean Journal of Food Science and Technology
• /
• v.45 no.2
• /
• pp.161-166
• /
• 2013

This study was carried out to investigate the physicochemical and microbiological characteristics of beef loin following microwave treatment at $4^{\circ}C$. Two types of microwave treatment were applied. i.e., continued microwave treatment (CW) and holding microwave treatment (HW). The L value increased, while a and b values were not significantly different among the samples as the storage time and microwave dose increased. The initial pH and after 3 days ranged from 5.51-5.74 and 6.32-6.51, respectively (p<0.05). The thiobarbituric acid value of all beef loins increased with increasing storage period and decreased with increasing microwave dose. The volatile basic nitrogen (VBN) content of the control was higher than that of the other samples and the VBN content decreased with increasing microwave dose. The total plate count of beef loins decreased with increasing microwave dose. This result indicated that T2 (100 W, HW) is most effective treatment with regard to the safety of beef loins without decreasing the physicochemical and microbiological characteristics.

• Journal of Marine Bioscience and Biotechnology
• /
• v.2 no.2
• /
• pp.113-119
• /
• 2007

In this study, we investigated antimicrobial and cytotoxicity effects of Gelidium amansii L., which using methanol, dichloromethane and ethanol were extracted and fractionated into four different types : methanol (GAMM), hexane (GAMH), butanol (GAME) and aqueous (GAMA). The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the solvent fractions, The methanol partition layer (GAMM) showed the strongest antimicrobial activities and cytotoxic effects on all cancer cell lines. We also observed quinone reductase (QR) induced effects in all fraction layers of GA on HepG2 cells. The QR induced effects of GAMM on HepG2 cells at $40{\mu}g/mL$ concentration indicated 2.5 with a control value of 1.0.