• Korean Journal of Ecology and Environment
• /
• v.45 no.1
• /
• pp.129-138
• /
• 2012

We investigated an ecological impact of drought simulated by discharge depletion on the water quality and benthic diatom community in the indoor experimental channel. As artificial substrates slide-glass was installed in acrylic channel for 16 days. Channels were supplied continuously with eutrophic lake water with a discharge rate of 6 L $min^{-1}$ in duplication during the colonized period. And then during the discharge depletion period, three discharge rates were provided: NDF (No depletion of flow rate (Control): 6 L $min^{-1}$), LDF (Low depletion of flow rate: 3 L $min^{-1}$) and HDF (High depletion of flow rate: 1 L $min^{-1}$). Environmental factors in the water, such as suspended solid, Chl-$a$ and nutrients concentration, were measured with periphytic algae including AFDM (ash free dry matter), Chl-$a$ concentration and cell density at 1-day intervals. Light intensity increased significantly with discharge depletion (F=229.5, p= 0.000). $NH_4$-N concentration was highest at HDF. Suspended solid in outflowing water decreased at HDF (88%), LDF (97%) and NDF (99%), compared to inflowing water (100 %). Chl-$a$ in substrates increased more than two times at LDF and HDF than NDF (F= 8.399, p=0.001). Also AFDM and benthic diatom density increased significantly at LDF and HDF than NDF (F=9.390, p=0.001; F=6.088, p=0.007). In all experimental groups, $Aulacoseira$ $ambigua$, $Achnanthes$ $minutissima$ and $Aulacoseira$ $granulata$ were dominant species accounting for greater than 10% of benthic diatom density. The most dominant species, $A.$ $ambigua$ was highest at LDF, followed by HDF and NDF (F=8.551, p=0.001). In conclusion, the effect of drought simulated by discharge depletion in an artificial stream ecosystem caused significant changes on water quality and benthic diatom biomass. This result provides a useful data to understand the effect of draught on stream ecosystem in situ.

• Journal of Korean Society of Steel Construction
• /
• v.17 no.5 s.78
• /
• pp.617-625
• /
• 2005

The brace is often used to resist lateral force such as that exerted by an earthquake. Because of buckling at bifurcation load, the brace shows unstable hysteretic characteristics in the plastic zone. Therefore, in this study, the brace with damper that consists of slit plates were suggested on the purpose of preventing buckling and increasing plastic deformation capacity. The experimental results regarding the brace member were analyzed and the feasibility was also examined.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.2235_2236
• /
• 2009

본 논문에서는 초음파 센서를 이용하여 3차원 마우스 시스템을 설계하였다. 마우스에 부착시킨 초음파 발신부와 모니터에 부착시킨 초음파 수신부를 이용하여 마우스와 모니터에 부착된 수신부사이의 거리를 측정한다. 측정한 거리는 무선네트워크를 이용하여 Host PC에 전송하고, Host PC는 측정거리와 위치 추정 알고리즘을 이용하여 마우스의 위치를 추정한다. 최소자승법을 이용하여 위치 추정 알고리즘의 모의실험을 수행하였으며, 모의실험 결과로부터 최소자승법을 3차원 마우스 시스템의 위치추정 알고리즘으로 활용할 수 있음을 확인하였다.

• Journal of Biosystems Engineering
• /
• v.21 no.1
• /
• pp.10-20
• /
• 1996

관개방제 기술의 변수 및 효력예측을 위한 컴퓨터 시뮬레이션 프로그램이 유화/산화액적의 부착율, 유충의 추계적 난보운동 및 무작위 농약흡수 이론을 이용하여 개발되었다. 시뮬레이션 결과는 밤나방 유충, Spodaptera frugiperda (J.E. Smith) (Lepidoptera : Noctuidae)을 이용한 방제효력 실험결과와 비교하였다. 이론치와 실험치는 서로 일치되었다. 방제율은 농약유효성분량이 증가함에 따라 증가하였으며 크로포(chlorpyrifos) 약제의 표준 권고량인 670g[AI]/ha에서 완전방제가 예상되었다. 유화액적(emulsion)은 상대적으로 직경이 큰 산화액적(dispersion) 보다 작물잎 표면에 부착이 어려워 낮은 방제율을 나타내었다. 액적직경이 방제효력에 미치는 영향은 목화작물에 대하여 낮은 약제량에서 뚜렷하게 나타났고, 그 영향은 약제량이 증가할수록 목화 및 옥수수 모두에서 저하되었다. 엽형계수는 작물의 엽상구조에 따른 액적의 부착 및 계류에 미치는 영향을 의미한다. 고찰된 관계방제기술의 영향요소에 대한 이해는 농약사용의 감소 및 효력의 증가에 중요한 역할을 한다.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2010.05a
• /
• pp.89-90
• /
• 2010

Three post-tensioned lightweight concrete beams were tested to examine the effect of the reinforcement index on the unbonded tendon stress at ultimate strength of the beams. The reinforcement index selected for main variables were 0.06, 0.15, 0.30. Test results showed that the stress of the unbonded tendons in the ultimate strength of the post-tensioned lightweight concrete beams can be conservatively evaluated using the empirical equations specified in ACI 318-08.

• Journal of Life Science
• /
• v.18 no.7
• /
• pp.952-957
• /
• 2008

We evaluated the effects of various factors (e.g., serum, inhibitors of protein synthesis, and cytoskeleton and protein kinases) on early PMA-induced THP-1 cell adhesion using an adhesion assay with Sulforhodamine B (SRB) staining, which was used to assess the proliferation of the attached cells. THP-1 cell adhesion to a plastic substrate was detected 1 hr after exposure to Phorbol 12-Myristate 13-Acetate (PMA) and peaked after 18 hr. At concentrations > 25 nM PMA, the level of adhesion did not change. Based on our preliminary results, we used 25 nM PMA and 5 hr of culture as standard assay conditions. Early PMA-induced cell adhesion was not affected by the presence of serum or PD 98059 in the culture medium, but was affected by the addition of PKC inhibitors and cycloheximide. In the presence of actin inhibitor with PMA, the cell adhesion increased when comparing with PMA treatment only. Thus, early PMA-induced adhesion of THP-1 cells does not require serum in the culture medium, MAP-kinase activation, or actin polymerization, but does require de novo protein synthesis and PKC activation. Our SRB-based cell adhesion assay may be used to screen other PKC inhibitors.

• KSBB Journal
• /
• v.16 no.1
• /
• pp.41-47
• /
• 2001

H. pylori is known to be a key pathogen of chronic gastric and duodenal ulcers. Bacterial adhesion to hosts is an essential step for bacterial infection and the inhibition of this adhesion provides a possible method for the treatment of the infection. The inhibitory effect of antibody lgY, produced from immunized hens with H. pylori antigen, was studied in vitro. The inhibition of H. pylori adhesion to AGS was as high as 90% using 0.5mg/ml of lgY, and almost 80% of the detachmentwas also achieved. The inhibitory effect of adhesion-inhibition candidates was investigated. Additives in combination with lgY increased the adhesion-inhibiting effect by about 30-50%. However, the adhesion molecules of H. pylori were varied and complex, therefore the further studies are necessary to develop an adhesion inhibitor and effective enough to be employed for the treatment of H.pylori, in vivo.

• Journal of the Korea Concrete Institute
• /
• v.26 no.4
• /
• pp.507-515
• /
• 2014

Although the construction joints of a concrete structure are properly treated with some measures, leakage has frequently occurred. A series of tests on the bond characteristics between new and old concrete were carried out in this study, assuming that the leakage at the construction joints has certain relationship with the bond of concrete. To assess the bond characteristics under various conditions, a number of specimens were made that have an interface between new and old concrete and bond strength, flexural strength and splitting tensile strength were measured. Main test variables are type and amount of mineral admixtures, treatment method of the interface and type of waterstops. In addition, the effects of placing interval between the concrete and of the age of the strength tests were investigated. The test results showed a slightly increased bond strength when applying mineral admixtures, which can be attributed to the interface filled with the calcium silicate hydrate that is formed by pozzolanic reaction. On the other hand, the bond strength was higher when the interface was treated rough and dry, and the roughness of a waterstop affected the bond capacity of the waterstop. Also, an assessment is required that considers the type of strength test because the bond strength varied according to the test methods.

• Journal of Periodontal and Implant Science
• /
• v.35 no.2
• /
• pp.475-490
• /
• 2005

본 실험은 흡연이 치주인대세포의 기능에 미치는 영향을 알아보기 위해 담배 부산물 중 하나인 니코틴이 지주인대세포의 부착과 성장 및 세포의 가역성에 미치는 영향을 관찰하였다. 니코틴이 치주인대세포의 부착에 미치는 영향을 관찰하기 위하여 치주인대세포에 니코틴을 투여한 후 1, 3, 6, 12, 24시간째 trypan blue 염색 후 부착된 세포수의 측정과 H&E stain후 형태학적 관찰을 하였고 니코틴이 치주인대세포의 성장에 미치는 영향을 관찰하기 위하여 치주인대세포에 니코틴을 투여한 후 1, 4, 7, 11, 14일째 trypan blue 염색 후 증식된 세포수의 측정과 H&E stain후 형태학적 관찰을 하였다. 또 니코틴의 세포독성효과의 가역성 평가를 위하여 니코틴을 투여하고 1, 4, 7, 11일째 니코틴이 없는 새로운 배지로 갈아주어 2일 더 배양한 후 trypan blue로 염색하여 세포수를 측정, 비교하였고 H&E 염색 후 형태학적 관찰을 하였다. 실험결과 니코틴 농도가 증가함에 따라 치주인대세포의 부착율은 감소되었고 24시간 배양 후 2mg/ml, 0.5mg/ml, 0.1mg/ml니코틴 투여군에서 통계학적으로 유의성 있는 부착억제가 관찰되었고(P<0.01) 형태학적 관찰결과 2mg/ml, 0.5mg/ml 니코틴 투여군에서는 대조해서 관찰되는 세포질의 확장이 관찰되지 않았다. 니코틴이 치주인대세포의 증식에 미치는 효과를 살펴본 결과 2mg/ml, 0.5mg/ml, 0.1mg/ml 니코틴 투여군에서 증식억제가 관찰되었고(P<0.01) 0.005mg/ml이하의 니코틴 투여군에서는 아무런 변화도 관찰되지 않았다. 14일 배양 후 형태학적 관찰결과 0.1mg/ml 니코틴 투여군에서 세포질내 공포를 관찰할 수 있었고 2mg/ml, 0.5mg/ml 니코틴 투여군에서는 세포의 괴사가 나타났다. 니코틴의 세포독성효과의 가역성평가결과 2mg/ml이상의 니코틴은 세포에 비가역적인 손상을 일으키며 0.5mg/ml, 0.1mg/ml 니코틴에 의한 세포독성은 초기에는 가역적이나 장기간 세포에 노출시키면 비가역적인 손상을 야기하는 것으로 나타났다.(P<0.05) 본 실험결과 담배의 구성성분 중 니코틴은 농도 의존적으로 치주인대세포의 부착과 증식에 영향을 주었고 니코틴의 치주인대세포에 대한 독성효과는 농도가 증가할수록, 시간이 지닐수록 비가역적으로 나타났다. 따라서 흡연은 치주조직재생에 영향을 미칠 것으로 생각되며 흡연의 기간과 정도가 치주질환의 심도 및 치주치료 후 불량한 치유반응과 관계가 있을 것으로 사료된다.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.14 no.5
• /
• pp.144-152
• /
• 2010

In this study, cement-based filler is used as an adhesive instead of organic adhesive, epoxy because there were problems under wet condition. First, the bond strength of cement-based filler was measured and the result was satisfied with KS F 4716. However, in case of wet condition, bond strength of epoxy adhesive decreased $0.73N/mm^2$ in 7 days and $0.84N/mm^2$ in 14 days from pilot test. This implies that there would be a problem on reinforced concrete structure in wet condition, such as tunnel and sewage box. In the second experiment, the flexural strength of RC beams with GFRP using different thickness of cement-based filler was investigated, and the result was indicated 113%, 66%, 75% increase in 10mm, 20mm, 30mm thickness, respectively. From the result, it was known that 10mm filler thickness produces stable bond performance.