• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.166-177
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• 2006

Purpose : This investigation was undertaken to evaluate the antiproliferation, atoptosis of Sam-nueng(Sparganii Rhizoma) extract using MCF-7 human breast cancer cells. Methods : MCF-7 cells were cultured in Dulbecco's modified Eagle's medium/F12(DMEM/F12) supplemented with 10 % fetal bovine serum(FBS; Gibco) and antibiotics. At varying times after extract treatment, cells were harvested with scraper and processed for analysis of protein expression, proliferation, cytotoxicity and apoptosis. Results : Our results show that the extract of Sam-nueng(Sparganii Rhizoma) strongly inhibits the proliferation of MCF-7 cells in a dose and time-dependent manner. Sulforhodamine B showed that the addition of Sam-nueng(Sparganii Rhizoma) extract reduced the viability of MCF-7 cells in a dose-dependent manner. Poly[ADP(ribose)] polymerase(PARP) which serves as a marker of cells undergoing apoptosis, a major substrate for caspase-3 was extensively cleaved in the Sam-nueng(Sparganii Rhizoma)-treated cells. Conclusion : So, we can conclude that Sam-nueng(Sparganii Rhizoma) can have an inhibitive effect on MCF-7 human breast cancer cells by variable mechanisms.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.1-13
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• 2006

Purpose : Polygonum cuspidatum extract is an oriental herb which has been used for uterine diseases. In this study, the effects of Polygonum cuspidatum extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Viability of Polygonum cuspidatum extract-induced ME-180 cells was measured by MTT assay. Apoptotic cells were visualized by EtBr/AcOr staining under fluorescent microscope. Nucleosomal DNA fragmentation was assayed by agarose gel electrophoresis. Cell cycle distribution and changes in mitochondrial membrane potential were observed by flow cytometry. Results : Polygonum cuspidatum extract induced ME-180 cell death in a dose- and time-dependent manner. In the cells treated with Pc, the population of cells at sub-G1 phase significantly increased, and the condensed nuclei, apoptotic bodies and nucleosome-sized DNA were detected. Moreover, reduction in mitochondrial membrane potential was detected. Conclusion : Polygonum cuspidatum extract inhibits the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity initiated by depolarization of mitochondria.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.295-304
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• 2006

Inflammatory conditions of the breast are uncommon and may be encountered in the puerperal and nonpuerperal setting. Nonpuerperal mastitis is less common and usually presents with inflammation and chronic abscess formation in the periareolar and peripheral region. These abscess have a high rate of recurrence and are often associated with an underlying disease state, such as diabetes, steroid treatment, trauma or other kinds of a deficient immune system. The diagnosis is suggested by the history of sudden onset and the presense of swelling, tenderness, heat, erythema, and in the case of abscess, fluctuation. Ultrasound can also be useful. Treatment consists of antibiotic administration or incision and drainage of the abscess and, in cases of retroareolar abscess, resection of entire underlying duct system. Recently, we have gotten good result from treating two cases of nonpuerperal mastitis and breast abscess which had treatment of antibiotics and drainage three times but recurred. After taking gamijipaesan(加味芷貝散) and acupuncture treatment, they have recovered and haven't recurred in 8 months. So it proves that herb medication and acupuncture are effective on nonpuerperal chronic mastitis and breast abscess.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.261-270
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• 2006

Purpose : The purpose of this study is to show the effect of acupuncture, moxibustion and magnetic innervation therapy for urinary incontinence symptoms. Methods : Ten female patients suffering from urinary incontinence had been given the treatments of acupuncture, moxibustion and magnetic innervation therapy for more than 8 times. Before and after treatment, they accomplished questionnaire for quality life survey and checked vaginal pressure measurement with perionometer. Results : The results were as follows 1) The mean${\pm}SD$ of Questionnaire score was significantly decreased from $35.40{\pm}11.88$ to $21.20{\pm}7.66$ after treatment(P<&0.047). 2) The mean${\pm}SD$ of maximum vaginal presssure was significantly increased from $48.80{\pm}25.35mmHg$ to $62.20{\pm}25.09mmHg$(p<&0.040). 3) The mean${\pm}SD$ of average vaginal presssure was significantly increased from $33.50{\pm}19.62mmHg$ to$43.10{\pm}16.49mmHg$(p<&0.015). Conclusion : Our results suggested that acupuncture, moxibustion and magnetic innervation therapy is effective for urinary incontinence symptoms.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.178-187
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• 2006

Purpose : The aim of this study was to investigate objective evaluation of dysmenorrhea. Methods : The study was conducted by 37 articles from year 1979 to 2003 on dysmenorrhea, scale and questionaire. The articles was limited by English, Human, and Clinical trial Results : 1. The 37 articles on dysmenorrhea evaluated by scale or questionaire have been published from 1979. There are 27 articles of randomized controlled trial among them. 2. Scale or question has confidence in the assessment of dysmenorrhea. 3. In the initial stage the article on dysmenorrhea was assessed by only one unidimentional scale. However in recent articles multidimensional scales are applied frequently. Moreover it is the modern trend that point scale such as 100mm VAS and MVRS(multidimensional verbal rating scale) are applied together in one article on dysmenorrhea. Conclusion : It can be suggested that scale or questionaire be the method for objective evaluation of dysmenorrhea.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.218-227
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• 2002

Pelvic Inflammatory Disease(PID) is a spectrum of inflammatory disorders of the female genital tract involving at least the endrometrium and may include the fallopian tubes, ovaries, and pelvic cavity. Women with PID acutely experience pain and are at risk for sepsis; however, the long-term complications such as chronic pelvic pain, organic changes like chronic salpingitis, adhesions, etc., and the significant increases in ectopic pregnancy and infertility are more important and hard to control. This study is about one patient who has chronic PID with inflammatory pelvic mass. The patient was treated with retention enema therapy which use herbs effective for reducing inflammation, resolution abdominal mass, promoting blood circulation. Also, treated with herbal medication of Danchisoyosan(丹梔逍遙散) and Moxibution at Zhongwan(中脘), Guanyuan(關元). After total treatment for three months, the patient's symptoms are improved and the size of inflammatory pelvic mass is reduced. This results indicates that the combination treatment, especially the retention enema therapy is effective on reducing symptoms of PID and the size of secondary inflammatory organic changes.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.202-207
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• 2002

Purpose : This study was designed to investigate the effect of herbal medication prescribed for patients with febrile diseases in the pregnancy. Method : We prescribed Kungsosankakambang for 18 patients who had got cold, at Wonkwang University Gwangju Oriental Hospital from January 2001 to September 2002. They had the symptoms of cough, sputum, snivel, headache, whole body pain, etc. Results : The most of patients were improved. This herbal medicine did 9 patients good right after dosage. And 8 patients have improved much in health.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.238-246
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• 2002

Recently we observed and treated a 49-year-old postmenopausal female with enlarged uterine myoma who taking through the hormone replacement therapy(H.R.T). We regard the cause of this case as insufficiency of kidney-yin(腎陰虛), stagnation or the phlegm and pathologic blood(痰瘀阻滯) and H.R.T. ln general cases, myomas grow in size if estrogen and progesterone is present and do not increase in size if estrogen and progesterone levels are low. So if a woman can get to menopause without having symptoms from the myomas, then it is likely that she will never have problems from the growths that require treatment. But Taking postmenopausal estrogen and progestin replacement therapy can cause myomas to grow. So we recommended her to stop the H.R.T. gave herbal-medicine and acupuncture therapy. Thus we could get a good result as follows. The uterine myomas were significantly declined but no change on it's characters. Hot flush and general conditions were also significantly improved And body weight was lessened for about 4.7kg. But vaginal dryness was appeared, So we planed another therapy for that symptom.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.282-294
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• 2006

Purpose : The purpose of this study is to report the effect of oriental treatments to postpartum depression Methods : We treated the patient who had postpartum depression and visited Joong-hwa oriental hospital. The patient in this case, 36-years-old female, was admitted for 15days(161th/May/2005-30th/May/2005) due to postpartum depression which was measured by EPDS(Edinburgh Postnatal Depression Scale) and BDI(Beck's depression Inventory). The symptoms of patient are insomnia, anxiety, palpitation, chest discomfort and depression. This patient was treated with Hominis Placenta Herbal acupuncture at CV4(Kwanwon, Guanyuan), CV6(Kihae, Qihai), B23(Shinsu, shenshu), GVl5(Amun, Yamen), herb medication, acupuncture, auricular acupuncture, moxa treatment, and so forth. Results : As a result, symptoms are remarkably alleviated. Conclusions : Hominis Placenta Herbal acupuncture is expected to have an effect on postpartum depression. After this, further approach and study on postpartum depression might be needed.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.208-217
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• 2002

We had treated one melasma patient occurred by pregnancy with oriental medicine's method and got a good result. Melasma is often occured in pregnancy and dysmenorrhea. Most common pathology of melasma in oriental medicine is Yin deficiency with Fire and Blood Stasis. The Fire and Blood Stasis are made by Qi stagnation which is occurred by depression and stress. So we used the methods of promoting Qi and Blood, increasing Yin with clearing heat, and the result is very successful. We found out that Oriental Gynecologic therapy is most appropriate for treating woman's melasma, and could provide the treatment model.