The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Anti-proliferative effect of Sam-nueng(Sparganii Rhizoma) extract on MCF-7 cells

삼릉(三稜) 추출물의 인간 유방암 세포 성장 억제 효과

  • Park, Kyong-Mi (Dept. of Oriental MedicineGraduate School of Dongshin Univ.) ;
  • Cho, Sung-Hee (Dept. of Oriental MedicineGraduate School of Dongshin Univ.) ;
  • Jeong, Kyoung-Ah (Dept. of Oriental MedicineGraduate School of Dongshin Univ.)
  • 박경미 (동신대학교 한의과대학 부인과학교실) ;
  • 조성희 (동신대학교 한의과대학 부인과학교실) ;
  • 정경아 (동신대학교 한의과대학 부인과학교실)
  • Published : 2006.02.28
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Abstract

Purpose : This investigation was undertaken to evaluate the antiproliferation, atoptosis of Sam-nueng(Sparganii Rhizoma) extract using MCF-7 human breast cancer cells. Methods : MCF-7 cells were cultured in Dulbecco's modified Eagle's medium/F12(DMEM/F12) supplemented with 10 % fetal bovine serum(FBS; Gibco) and antibiotics. At varying times after extract treatment, cells were harvested with scraper and processed for analysis of protein expression, proliferation, cytotoxicity and apoptosis. Results : Our results show that the extract of Sam-nueng(Sparganii Rhizoma) strongly inhibits the proliferation of MCF-7 cells in a dose and time-dependent manner. Sulforhodamine B showed that the addition of Sam-nueng(Sparganii Rhizoma) extract reduced the viability of MCF-7 cells in a dose-dependent manner. Poly[ADP(ribose)] polymerase(PARP) which serves as a marker of cells undergoing apoptosis, a major substrate for caspase-3 was extensively cleaved in the Sam-nueng(Sparganii Rhizoma)-treated cells. Conclusion : So, we can conclude that Sam-nueng(Sparganii Rhizoma) can have an inhibitive effect on MCF-7 human breast cancer cells by variable mechanisms.

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