• Microbiology and Biotechnology Letters
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• v.35 no.4
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• pp.334-338
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• 2007

The disinfection effects of trichloroisocyanuric acid (TICA), calcium hypochlorite (CH), and Bromochlorodimethylhydantoin (BCDMH) on various bacteria in aqueous suspension were comparatively characterized at various concentrations and exposure times of each disinfectant. When various bacteria cells ($10^8\;CFU/ml$) including Escherichia coli, Pseudomonas aeroginosa, Enterococcus faecalis, Bacillus cereus, Legionella pneumophila, and Staphylococcus aureus were exposed with a solution containing 8 ppm each of TICA, a 99% of the initial cells were killed in 60 sec, 368 sec, 372 sec, 506 sec, 812 sec, and 909 sec, respectively. In addition, the minimum exposure time required to kill 99% of E. coli ($10^8\;CFU/ml$) with 8 ppm of each TICA, BCDMH and CH was measured at 60 sec, 114 sec, and 7,100 sec, respectively. These comparative studies demonstrate that disinfection efficacy is highly variable depending on microbial species as well as disinfectant type, concentration, and exposure time.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.1
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• pp.1-8
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• 2009

EMMC(Entrapped Mixed Microbial Cell) process which is a kind of active cell immobilizing method was applied to treat fisheries processing wastewater biologically. Kinetic constants were calculated for organic and nitrogen removal and effect of effluent recycling on system performance was evaluated also. Yield coefficient, Y showed relatively low value compared with Y value obtained from conventional activated sludge process. It means that EMMC process can reduce amount of excess sludge significantly compared with conventional activated sludge process. Endogenous respiration coefficient $k_e$ of EMMC process also showed relatively low value compared with that of conventional activated sludge process. Yield coefficient Y, endogenous respiration coefficient $k_e$ and half saturation constant $k_s$ obtained from EMMC process in terms of nitrification were compared with reported value from literature based on suspended growth nitrification system. The value of Y obtained from this study has no difference compared with values obtained from literature review and $k_e$ of this study was low but $k_s$ of this study was high compared than values obtained from suspended growth nitrification system. To evaluate the effect of internal recycling on system performance, system was operated with internal recycling ratio of 1.5Q, 2.0Q, 2.5Q and 3.0Q. increase of internal recycling ratio effect more greatly on improvement of denitrification efficiency than that of nitrification efficiency. Accordingly, optimization of internal recycling ratio has to be based on improvement of anoxic reactor performance.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.207-211
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• 2011

하천공사의 매립 및 준설 시 뿐만 아니라 최근에는 호우 시에도 발생하는 고농도 오탁수의 유출 확산으로 인하여 주변 수역의 수산자원 및 자연환경에 심각한 영향을 미치고 있다. 특히 유사는 하천과 호소에 유입되어 탁도를 증가시켜 수자원의 이용가치를 저하시키며, 박테리아나 각종 미생물의 이동 매개체가 되고 있다. 또한 퇴적된 유사는 유량이 증가하면 다시 침식되어 부유상태가 되며 이때에 영양염류나 독성물질이 재용출 되기도 하는 등 하천의 물리 및 토양환경의 장 단기적인 변화를 유발하는 원인이 된다. 결국 유사는 다양한 방식으로 하천과 호소의 생태환경에 큰 변화를 초래한다. 부유토사의 발생 가능한 피해의 범위와 정도는 유역의 부유토사 발생율과 하천, 호소 퇴적물의 성분종류와 입도분포, 그리고 유속에 따라 결정된다. 따라서 탁수의 영향범위를 예측하고, 악영향이 예측될 경우에는 탁도를 저하시킬 수 있는 오탁물질의 저하기법을 적용하여 그 대책을 수립하여야 한다. 현재 정부가 역점을 두고 있는 4대강 살리기 사업 중 하나인 하천 준설 사업 시 발생되는 고농도 오탁수는 주변 수역의 수자원 및 자연환경에 심각한 영향을 미치므로 자연환경의 보존, 해양자원을 보호하기 위하여 오탁물질의 제어가 반드시 필요한 실정이다. 기존의 오탁 저감시설이나 방법의 경우 오탁물질의 확산을 제어하는데 한계가 있고 오탁물질의 종류와 관계없이 일률적으로 시공함으로서 오탁제어 효과가 의문 시 되고 있다. 이러한 문제점을 개선하기 위하여 오탁물질의 확산 범위를 줄이고 침강효율을 높이는 방법을 찾아 오탁확산 제어 효율을 개선할 필요가 있다. 본 연구에서는 오탁물질 제어 시 2차 응집물로 인한 오염이 발생하는 않는 천연광물 제올라이트를 이용한 실내실험을 통하여 고농도 탁수의 탁도 저감효율을 파악하여 고농도 탁수의 탁도 저감에 대한 제올라이트의 적용 가능성을 살펴보았다. 4대강 살리기 사업 중인 OO강 OO공구의 OO보 현장에서 하천 준설 시 발생하는 고농도 탁수를 이온을 첨가한 제올라이트를 이용하여 확산제어에 관한 효과를 검토한 결과 2,000NTU 정도의 고농도로 유하하는 탁수에 대하여 제올라이트 혼합 후 20분이 경과한 시점에 제거효율이 90%이상으로 제올라이트의 탁수저감 효과가 대단히 크다는 것을 알 수 있었다.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.380-384
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• 2016

홍수완화의 관점에서 강을 관리하기 위하여 수자원의 이용과 생태계보전, 하천흐름과 형태학적 변화들에 대하여 충분히 이해하고 합리적으로 설명하는 것이 필요하다. 최근에 수변지역에서 발달된 식생은 홍수시에 감속영역과 생물들의 피난처를 제공하는 것 이외에 횡단방향의 혼합작용을 활성화하여 유사와 식물의 씨앗 및 입자성 유기물(POM)을 포착하는 기능을 하고 특히 흐름과 유사이송 및 하도 지형변화에 큰 영향을 주기 때문에 중요하다. 입자성 유기물(POM)은 하천생태계를 지탱하는 에너지원으로서 다양한 형태로 존재하고 미생물의 분해를 받아 무기화된 식생의 번무와 물질순환의 시발점이 되기도 하지만 현재까지 식생영역 내에서 그 공급과정에 관련이 있는 운동기구에 관한 연구는 아직 부족한 실정이다. 따라서 본 연구는 식생영역의 유사퇴적 및 분급작용, 입자성 유기물의 포착을 일으키는 원인과 흐름의 특징 중 식생영역 내에서 흐름방향으로 감속에 주목하여 수리 모형실험을 실시하였고 수변식생에서 부유사와 POM의 퇴적과정에 대하여 검토 및 모델링을 실시하였다. 수리 모형실험 결과 CPOM과 FPOM 모두 유사의 퇴적없이 그 자체로는 퇴적이 되지 않았고 수변식생의 종방향 이송의 경우 식생에 의해 퇴적된 부유사가 사련 형태로 형성이 되어 CPOM이 사련의 배후에서 캡쳐 되었다. 또한 두가지 샘플 움직임의 상호작용은 사련의 파고와 파장의 전파속도를 감소시켰다. 식생지역에서 횡방향 분산의 경우에 대해서는 각각의 유사 크기에 대한 퇴적물의 능선은 식생의 경계를 따라 형성되었고 운동의 범위는 유사 퇴적의 능선에 의해 촉진되어 횡방향으로 확산하며 확장되었다. 이러한 결과를 바탕으로 제한된 경험적인 지식보다 오히려 실험을 통하여 식생을 동반하는 장소에서 유사와 POM의 거동특성 차이 및 간섭작용을 명확히 한 후 현장에서 관측된 현상과 비교 검증이 필요하다고 사료되며 추후에 운동기구를 모델링 및 업그레이드 해 나가는 것이 앞으로의 하천생태계 예측평가에 필요하다고 판단된다.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.3
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• pp.143-148
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• 2012

This study was performed to investigate the concentration variation of airborne bacteria and endotoxin by the temperature in the compost pile in order to identify the generation characteristics of biological factors in the exhaust gases generated from lab-scale sludge compositing reactors (0.06 $m^3$ of total reactor volume). Airborne bacteria showed the highest concentration of generation ($1.03{\times}10^5\;CFU/m^3$) in the composting reactor without mechanical agitation, and similar change tendency to temperature variation of composting, but somewhat lower statistical significance (p>0.05). In the compost reactor with mechanical agitation, endotoxin showed similar generation characteristic to temperature variation of composting (statistical significance; p<0.05) and the highest generation concentration to 1,415 EU/$m^3$. Mechanical agitation of the composting process affected activity of microorganism and positive generation of endotoxin in exhaust gases. Endotoxin and airborne bacteria showed similar tendency of generation, especially the highest statistical correlation was observed in the compost reactor without mechanical agitation (statistical significance: p<0.01).

• Journal of Environmental Science International
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• v.6 no.1
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• pp.61-67
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• 1997

Heavy metal adsorption by microbial cells is an alternative to conventional methods of heavy metal removal and recovery from metal-bearing wastewater The waste Sac-chuomyces cerevisiae is an inexpensive, relatively available source of biomass for heavy metal biosorption. Biosorption was investigated by free and immobilized-S. cerevisiae. The order of biosorption capacity was Pb>Cu>Cd with batch system. The biosorption parameters had been determined for Pb with free , cells according to the Freundlich and Langmuir model. It was found that the data fitted reasonably well to the Freundlich model. The selective uptake of immobilized-S. cerevisiae was observed when all the metal ions were dissolved in a mixed metals solution(Pb, Cu, Cr and Cd). The biosorption of mixed metals solution by immobilized-cell was studied in packed bed reactor. The Pb uptake was Investigated in particular, as it represents one of the most widely distributed heavy metals in water. We also tested the desorption of Pb from immobilized-cell by us- ing HCI, $H_2SO_4$ and EDTA.

• Food Science and Preservation
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• v.16 no.4
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• pp.573-578
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• 2009

Microbial contamination levels in a fresh-cut leafy vegetable processing plant were evaluated. Total plate counts of samples collected from the walls, equipment, and raw materials ranged from $10^1{\sim}10^2$ CFU/100 $cm^2$, $10^0{\sim}10^4$ CFU/100 $cm^2$, and $10^4{\sim}10^6$ CFU/g, respectively. No coliforms were detected on walls; however, equipment and raw materials contained coliforms in concentrations ranging from ND (not detected)to $10^2$ CFU/100 $cm^2$ and $10^4{\sim}10^5$ CFU/g, respectively. Additionally, total plate counts for falling and floating bacteria in the processing plant were $10^0{\sim}10^1$ CFU/plate and $10^1{\sim}10^3$ $CFU/m^3$, respectively. Pathogenic microorganisms such as Escherichia coli, Salmonella spp, Staphylococcus aureus, or Listeria monocytogenes were not detected on walls, equipment, or raw materials. Overall, the results of this study indicate that hygiene control in the fresh-cut processing plant should be improved.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.6
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• pp.687-692
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• 2006

This experiment was performed to evaluate the characteristics of BNR system performance, behavior of pollutants as organic and nitrogen at each basin and the effects of C/N ratio on biological nitrogen removal with methanol as an external carbon source for a low C/N ratio municipal wastewater. A lab-scale $A_2O$ system by employing the aerobic basin with the fluidized polyurethane media, which was $10{\sim}20$ mm rube type like a sponge, was used. The aerobic basin was hybrid type to be suspended and fixed biomass. The obtained results from this study were as follows; When no methanol was added, suspended biomass was 3 times more than that of the fluidized media in this system(total biomass 80 g). Biomass growed by an external carbon was firstly attached on media, and then suspended. $COD_{Cr}$ concentration for the effluent was a range of 13 to 29 mg/L regardless of pouring an external carbon. For nitrogen, the effluent concentration was $20.0{\sim}35.9mg/L$(removal efficiency; 18%) in case of no addition of an external carbon, but was $2.5{\sim}9.0mg/L$(removal efficiency ; $71{\sim}83%$) with addition of methanol. For the characteristics of pollutants removal, most of $COD_{Cr}$ were removed at the anaerobic basin when no external organic carbon was added, and were removed at the anoxic basin in case of adding external organic carbon but at the aerobic basin in case of adding excess external organic carbon. On the other hand, most TIN(total inorganic nitrogen) were removed at the anaerobic basin when no external organic carbon was added, but when an external organic carbon was added, they were removed at the anaerobic basin under unstable condition and at the anoxic basin under stable condition.

• Journal of Korea Water Resources Association
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• v.50 no.11
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• pp.715-723
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• 2017

Extracellular Polymeric Substances (EPS) in the water environment assemble fine, colloidal particles, such as clays, microorganisms and biomass, in large flocs, which are eventually subject to sedimentation and deposition and determine water/sediment quality and quantity. This study hence aimed to investigate the way that water and colloidal chemistry affects EPS-mediated flocculation of colloidal particles, using a jar-test experiment. Especially, ionic strength, divalent cation and humic substances concentrations were selected as experimental variables in the jar-test experiments, to elucidate their effects on EPS-mediated flocculation. A higher ionic strength increased flocculation capability, reducing electrostatic repulsion between EPS-attached colloidal particles and enhancing particle aggregation. 0.1 M NaCl ionic strength had higher flocculation capability, with 3 times larger floc size and 2.5 times lower suspended solid concentration, than 0.001 M NaCl. Divalent cations, such as $Ca^{2+}$, built divalent cationic bridges between colloidal particles and EPS (i.e., $colloid-Ca^{2+}-EPS$ or $EPS-Ca^{2+}-EPS$) and hence made colloidal particles to build into large, settelable flocs. A small $Ca^{2+}$ concentration enhanced flocculation capability, reducing suspended solid concentration 20 times lower than the initial dosed concentration. However, humic substances, adsorbed on colloidal particles, reduced flocculation, because they blocked EPS adsorption on colloidal particles and increased negative charges and electrostatic repulsion of colloidal particles. Suspended solid concentration in the tests with humic substances remained as high as the initial dosed concentration, indicating stabilization rather than flocculation. Findings about EPS-mediated flocculation in this research will be used for better understanding the fate and transport of colloidal particles in the water environment and for developing the best management practices for water/sediment quality.

• Journal of the Mineralogical Society of Korea
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• v.26 no.4
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• pp.219-228
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• 2013

To understand characteristics of biogeochemical corrosion for the metal canisters that usually contain the radioactive wastes for a long-term period below the ground, some metal materials consisting of cast iron and copper were reacted for 3 months with D. desulfuricans, a sulfate-reducing bacterium, under a reducing condition. During the experiment, concentrations of dissolved metal ions were periodically measured, and then metal specimen and surface secondary products were examined using the electron microscopy to know the chemical and mineralogical changes of the original metal samples. The metal corrosion was not noticeable at the absence of D. desulfuricans, but it was relatively greater at the presence of the bacterium. In our experiment, darkish metal sulfides such as mackinawite and copper sulfide were the final products of biogeochemical metal corrosion, and they were easily scaled off the original specimen and suspended as colloids. For the copper specimen, in particular, there appeared an accelerated corrosion of copper in the presence of dissolved iron and bacteria in solution, probably due to a weakening of copper-copper binding caused by a growth of other phase, iron sulfide, on the copper surface.