• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.308-311
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• 2007

황해의 25개 조사정점의 표층수에 대한 해양환경요인 및 식물플랑크톤 현존량의 월별 조사결과를 종합하여 주성분분석(PCA)을 실시한 결과 주성분 I에서는 변화를 26.6% 설명할 수 있으며, 이중 질산질소가 가장 많이 기여하였고, 이어서 용존무기질소, 규산규소, 수온, 용존산소, 암모니아질소 순으로 기여 하고 있었다. 주성분 II에서는 20.3%를 설명 할 수 있으며, 이중 용존산소가 가장 많이 기여하였고 그 외 엽록소 a, 수온, 염분, 규산규소, 식물플랑크톤 현존량 순으로 기여 하였다. 주성분 III에서는 15.1%를 설명 할 수 있으며, 이중 인산인이 가장 많이 기여하였고 그 외 염분, 아질산질소, 수온, 용존산소, 질산질소, 용존무기질소 순으로 기여하였다. PCA에 이용된 항목 중 부유물질은 제외한 모든 항목은 주성분 I, II, III에 5%이하 유의수준에서 의미 있는 상관성이 있었다. 주성분 I은 질산성질소, 용존무기질소, 규산규소, 인산인과는 긍정적인 상관성(p<0.0001)을 갖고, 수온, 암모니아질소와는 부정적인 상관성을 보였다(p<0.001). 주성분 II에서는 용존산소, 엽록소 a, 식물플랑크톤 현존량은 긍정적 상관성을 나타내었고, 수온, 염분, 규산규소와는 부정적인 상관성을 보였으며(p<0.0001), 주성분 III에서는 염분, 수온, 질산질소, 용존무기질소, 식물플랑크톤 현존량과는 긍정적인 상관성(P<0.0001)을 나타내었고 인산인, 아질산질소, 용존산소는 부정적인 상관성을 나타내었다 (p<0.01). 공간적인 분포특성은 조사정점에 따라 항목별 결과에 대한 분산이 커서 일정한 특성을 도출하기가 어려운 상태이나 대체로 PCA II축을 기준으로 상부인 $1/4{\sim}2/4$분면에 북부와 중부의 조사정점이 위치하고 음의 방향인 $3/4{\sim}4/4$분면에 중부와 남부의 조사정점이 위치하고 있어 북측의 307선과 308선 및 중부의 309선과 310선 그리고 남측의 311선과 312선으로 구분되어지는데 그 중에서 태안반도의 연안역인 307선의 03점은 계절에 변화가 가장 커서 분산의 폭이 심한 상태로서 다른 조사정점들과 구분되어진다. 전반적으로 북측해역에서는 용존산소, 엽록소 a, 식물플랑크톤 현존량이 남측해역에 비해 상대적으로 많은 반면, 남측해역은 수온과 염분 및 규산규소가 높은 상태이었다. 북쪽인 307선과 308선은 연안역과 외해에서 상대적으로 영양염류가 높았으며, 중부인 309선과 310선은 연안역과 준 외해역인 07점과 09점에서 영양염류가 높았었고, 남부 해역인 311선과 312선에서는 연안역에서 영양염류가 상대적으로 높은 경향을 나타내었다. 즉 황해 동부는 연안역에서는 조석간만의 차가 심하고, 새만금 및 시호호등과 같은 대규모의 간척 매립으로 육상의 오염원이 자정작용을 거치지 못하고 직접 연안으로 유입되고 있는 실정이다. 또한 10여 년 전부터 육지의 모래부족으로 다량의 바다 모래가 채취되어 왔고 그 량이 점차 증가추세이며, 중부외해역에서는 각종 폐기물 투기로 점차 영양염류의 부하량이 증가되고 있어 특히 질소계와 인의 영양염류 농도가 높아지고 있는 추세이다. 시간적 변동특성은 전반적으로 순환하는 형태를 유지하고 있다. 2005년 2월에는 질산질소, 용존무기질소, 규산규소, 인산인, 용존산소가 높았었으며 4월로 접어들면서 항목 간에 분산되는 경향을 보이면서 수온, 엽록소 a 및 식물플랑크톤이 증가 하면서 해역에 따라 좌우로 분산되는 경향을 나타내었고 6월에는 아질산질소를 제외한 영양염류가 감소하는 경향을 보였다. 8월에는 6월에 비해 수온이 높아지고 규산규소, 질산질소, 용존무기질소 등이 약간 증가추세이었으며 10월로 접어들면서 증가추세가 더 높아졌었고 12월에는 질산질소, 용존무기질소, 규산규소, 인산인, 용존산소가 높아졌었다. 2006년 4월에는 2005년 4월에 비해 수온, 엽록소 a, 식물플랑크톤의 현존량이 높아져서 전년 동시기와 약간 다른 양상을 보이고 있었다. 즉 동계인 2월을 시작으로 반 시계 또는 시계 방향으로 순환하는 형태를 유지하고 있으며, 4월은 2개년 비교해 보았을 때 해마다 해양환경에 따라 그 순환 정도 및 형태가 다를 것으로 추정된다.

• Korean Journal of Ecology and Environment
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• v.38 no.3 s.113
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• pp.372-381
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• 2005

This study was conducted to test the efficiency of water quality improvement using the dissolved air flotation (DAF) technique in a shallow eutrophic reservoir. The application of DAF was followed by the addition of a chemical coagulant (poly aluminum chloride; PAC). The experiment was conducted in the mesocosm scale (wide ${\times}$ length ${\times}$ depth: 6 m ${\times}$ 6 m ${\times}$ 3 m). Suspended solids (SS) and volatile SS (VSS) concentration decreased by 54 ${\sim}$ 71% and 57 ${\sim}$ 79% of the initial concentrations, respectively. Total phosphorus and Chl- a concentration also decreased by 74 ${\sim}$ 92% and 54 ${\sim}$ 98%, respectively. BOD decreased by>86% while COD decrease ranged 29 ${\sim}$ 63%. Dissolved inorganic P (DIP) and dissolved total P (DTP) concentration decreased by 34 ${\sim}$ 88% and 62 ${\sim}$ 88%, respectively. After DAF application further onto the sediment, DIP-release rates from the sediment decreased by 17% (0.82 ${\to}$ 0.68 mg $m^{-2}$$day^{-1}$ in the oxic condition and 23% (2.27 ${\to}$ 1.76 mg $m^{-2}$$day^{-1}$) in the anoxic condition, compared to the release rate from the untreated sediment. DTP-release rate from both the oxic and anoxic sediments also decreased by 33% (5.62 ${\to}$ 3.78 mg $m^{-2}$$day^{-1}$) and 20% (6.23 ${\to}$ 4.99 mg $m^{-2}$$day^{-1}$), respectively. These results suggest that the DAF application both to the water column and onto the sediment be effective to improve water quality by removing particulate matters in the water column as well as reducing P-release from the sediment.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.524-540
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• 2013

After the construction of the Geum River estuary dam, we investigated the ecological property of phytoplankton community with physicochemical property in 9 stations of Geum River estuary 4 times seasonally in 2010 about the environmental changes through the change of phytoplankton. Physicochemical property seems to show eutrophication which developed from the tendency of accruing of the nutrients (those involved in intermittency of freshwater which flows from the dike and the surrounding cities), caused by the changing of tidal flow, the decreasing of the inflow of seawater after the construction of the Geum River estuary dam (dike). A total of 233 species of species composition of phytoplankton emerged, showing a highly increased result compared to that of the former research. The reason for the increase in number is considered as a phenomenon resulting from the mix of blackish and freshwater marine species in the flow of intermittency of freshwater discharge. Also, the standing crops of phytoplankton considerably increased compared to when the dike had not yet been built. Highly affected by the discharge of occasional freshwater, the diatoms of blackish water zone dominated in spring and winter, while freshwater species appeared to dominate in summer and autumn. The species diversity showed over 2.0 except in winter season. After a cluster analysis, the result showed that the Geum River estuary was influenced by runoff from cities like Gunsan, the sewage which flows from Gyeongpo River, and the intermittency of freshwater discharge from the Geum River estuary dam.

• Journal of Animal Environmental Science
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• v.14 no.1
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• pp.31-38
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• 2008

Ammonia gas is one of the malodorous gases from swine production facilities, such as manure storage tank, manure fermentation facilities, and livestock houses, etc. Ammonia gas from swine house is being emitted at relatively low concentrations throughout the year. Therefore, livestock facilities were continuously ventilated to supply fresh air for respiration of the animals internal the livestock facilities. The swine facilities need very high ventilation rate to control the inside environmental conditions. The deodorization system of the livestock facilities must be developed considering the ventilation rates. The odor abatement system was installed in order to improve the internal environment of the naturally ventilated growing-finishing pig house. The system which distributes the deodorized air into inner space of the swine house by using plastic duct was installed. Since the internal environment, effected by the operation of the odor abatement system, is monitored by closing the winch curtain installed on the side wall of the pig house, the experiment was practiced at the season when the internal environment becomes aggravated, winter. The effects on the improvement in the internal environment of swine house by operating the odor abatement system are as follows ; 1. By re-distributing the air which was deodorized by the odor abatement system installed in the pig house, the result showed that the concentration of ammonia gas is decreased approximately 33.3% compared with that before operating odor abatement system. 2. The effect on the pig house's ammonia gas reduction was found that the ventilation rate was less than $0.5m^3$/min head. The effect of the operation of the odor abatement system showed to be scarce when the ventilation rate increases because of the influx of external fresh air makes the quantity of diluted air more than those of the odor abatement system. 3. The perishment rate of the pigs which were brooded until slaughtering decreased about 3.8% by operating the odor abatement system in the growing-finishing pig house. Also, after operating the odor abatement system, the stinging of the eyes, suspension dust, etc were decreased when going into swine house for management.

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.161-171
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• 2009

The present study was performed to describe the influence of water temperature and salinity on filtration rates of the venus clam, Gomphina veneriformis, a suspension-feeding (filter-feeding) bivalve species. The calmswere collected from the eastern coastal area of Sokcho, Gangneung and Jumunjin at Kangwon-do, Korea, during December 2006 and May 2007. Isochrysis galbana (KMCC H-002) cells as food organisms were indoor-cultured by f/2 medium, and were used to measure the filtration rate of clam. Filtration rates of clam were measured by indirect method. Cell concentration of food organisms were determined by direct counting cells used the hemacytometer under the light microscope. The filtration rates of clams by water temperature sharply increased with temperatures up to $15^{\circ}C$ as optimum temperature and above this temperature, the filtration rates decreased exponentially. Venus clams showed very low filtration rates at low salinity (10-15 psu) and maximum values at high salinity (30-35 psu). Regardless of water temperature and salt change, 2-year class clams showed high filtration rates, but low in 4-year-class. Polynomial regression curves with water temperature were shifted to the left in low temperature region. Thermal coefficient $Q_{10}$ values showed much higher values at low temperature range than at high temperature range, too. These results indicate that the venus clam is more sensitive in cold water. Polynomial regression curves with salinity were shifted to the right in high saline region. According to this study, the venus clam Gomphina veneriformis, subtidal filter-feeding bivalve, was the stenothermal organism, inhabited mainly in low temperature and the stenohaline, in high saline waters.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.3
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• pp.180-190
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• 2014

It has been customary to exclude top 10-20% of velocity profiles in the Acoustic Doppler Current Profiler (ADCP) measurement due to side lobe effects at the boundary. To better understand the mixing in the Yeongsan estuary, the freshwater advection speed (FAS) was recovered from highly contaminated ADCP data near the surface. The velocity profiles were measured by using ADCP at two stations in the Yeongsan estuary during August 2011: one was located in front of the Yeongsan estuarine dam and the other was deployed near Goha Island. The FAS was recovered from the ADCP data set by applying rigorous post-processing methods and compared with the sediment advection speed (SAS). The SAS was determined by the peak time difference of suspended sediment concentration between two stations in the channel, divided by the distance of two stations. The FAS and the SAS showed very similar value when the freshwater discharge was greater than $2.0{\times}10^7$ ton and the SAS was a bit greater when the freshwater discharge was smaller. Since the FAS was on average about 0.8 m/s greater than the velocity at 0.8 of water depth from the bottom, the net discharge, estimated with recovered FAS and integrated over water depth and tidal cycle, was directed seaward during the high discharge contrary to the onshore direction of the net discharge estimated with 0.8 of water depth from the bottom. Moreover, the velocity shear and Richardson number changed when the FAS was used. Thus, the importance of the true FAS is appreciated in the investigation of the surface layer stability. If currents, temperature and salinity were observed for longer time in the future, it could be possible to more accurately understand the formation and decay of stratification as well as the suspended sediment transport processes.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.3
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• pp.271-277
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• 2008

The biofilm of white-rot fungi fully exposed in atmosphere are that operation is easy, management cost and energy waste is low. To develop biofilm of white-rot fungi fully exposed in atmosphere, basic test are as follows. To select most effective microoganism species, investigated treatment characteristics of wastewater containing non-biodegradable material for three species of white-rot fungi(Phanerochaete chrysosporium PSBL-1, Phanerochaete chrysosporium KCTC 6147, Trametes sp. KFCC 10941) and activated sludge. And then investigated attached and detached biomass of selected white-rot fungi species on HBC ring surface. Among the three strains tested, P. chrysosporium PSBL-1 and P. chrysosporium KCTC 6147 showed higher efficiency for organics removal than Trametes sp. KFCC 10941, and P. chrysosporium PSBL-1 showed higher efficiency for nitrogen removal than P. chrysosporium KCTC 6147 and Trametes sp. KFCC 10941. Respectively, 51$\sim$59.8%, 57.5$\sim$60.3% of NBDCOD was removed for P. chrysosporium PSBL-1 and P. chrysosporium 6147 in pH 3.5$\sim$5.5. TN removal efficiency showed 39.3$\sim$85.3%, 3.4$\sim$7.6% for P. chrysosporium PSBL-1 and P. chrysosporium 6147 in pH 4.5$\sim$11.5 respectively. Considered that white-rot fungi remove organism and nitrogen simultaneously, the microorganism selected white-rot fungi P. chrysosporium PSBL-1. White-rot fungi P. chrysosporium PSBL-1 attached on HBC ring surface 4,538 mg/L, 4,546 mg/L, 4,531 mg/L after 5 minutes, 4,575 mg/L, 4,573 mg/L, 4,568 mg/L after 10 minutes from initial MLSS 4,600 mg/L in pH 4, 7 and 10 respectively. Also detached biomass is negligible from right after attachment to 10 day in pH 4, 7 and 10.

• Journal of Environmental Impact Assessment
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• v.29 no.3
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• pp.157-181
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• 2020

Sediments from rivers, lakes and marine ports serve as end points for pollutants discharged into the water, and at the same time serve as sources of pollutants that are continuously released into the water. Until now, the contaminated sediments have been landfilled or dumped at sea. Landfilling, however, was expensive and dumping at sea was completely banned due to the London Convention. Therefore, this study applied contaminated sedimentation soil of 'Royal Palace Livestock Complex' as soil purification method. Soil remediation methods were applied to pretreatment, composting, soil washing, electrokinetics, and thermal desorption by selecting overseas application cases and domestically applicable application technologies. As a result of surveying the site for pollutant characteristics, Disolved Oxigen (DO), Suspended Solid (SS), Chemical Oxygen Demand (COD), Total Nitrogen (TN), and Total Phosphorus (TP) exceeded the discharged water quality standard, and especially SS, COD, TN, and TP exceeded the standard several tens to several hundred times. Soil showed high concentrations of copper and zinc, which promote the growth of pig feed, and cadmium exceeded 1 standard of Soil Environment Conservation Act. In the pretreatment technology, hydrocyclone was used for particle size separation, and the fine soil was separated by more than 80%. Composting was performed on organic and Total Petroleum Hydrocarbon (TPH) contaminated soils. TPH was treated within the standard of concern, and E. coli was analyzed to be high in organic matter, and the fertilizer specification was satisfied by applying the optimum composting conditions at 70℃, but the organic matter content was lower than the fertilizer specification. As a result of continuous washing test, Cd has 5 levels of residual material in fine soil. Cu and Zn were mostly composed of ion exchange properties (stage 1), carbonates (stage 2), and iron / manganese oxides (stage 3), which facilitate easy separation of contamination. As a result of applying acid dissolution and multi-stage washing step by step, hydrochloric acid, 1.0M, 1: 3, 200rpm, 60min was analyzed as the optimal washing factor. Most of the contaminated sediments were found to satisfy the Soil Environmental Conservation Act's standards. Therefore, as a result of the applicability test of this study, soil with high heavy metal contamination was used as aggregate by applying soil cleaning after pre-treatment. It was possible to verify that it was efficient to use organic and oil-contaminated soil as compost Maturity after exterminating contaminants and E. coli by applying composting.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.163-169
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• 2001

OSBA(oocytes-sperm binding assay) is a tool developed for rapid test of optimal condition of IVF medium and protein source by binding ability of mouse sperm and egg. Mouse oocyte-cumulus complexes were prepared by removing of the cumulus cells with 0.1% hyaluronidase. 10$\pm$2 oocytes per 30 ${mu}ell$ medium drop were inseminated with 3 ${mu}ell$ sperm suspension and were cultured f3r 3 hours and 24 hours, respectively. And the oocytes were recovered gently and the No. of sperm bound on oocytes were counted. In the Exp. 1, the ratio of oocytes bound with one sperm at least were 60.2%(50/83), 2%(2/77) and 100%(79/79) in the medium with no protein, FBS(15%, v/v) and BSA(0.4%. w/v), respectively, Fetal bovine serum(FBS) seriously inhibited sperm binding on oocyte, although bovine serum albumin(BSA) promoted the binding ability. The inhibiting effect of FBS was dependent on the concentration of FBS. The sperm binding ability according to oocyte maturity was tested in the Exp. 2. There was no significant difference between Met. II (mature) and Met. I (intermediate mature) oocytes in the number of oocytes bound with sperm and the number of sperm bound on oocytes. Finally, in Exp. 3, two batches of Ham's F10 medium with good and poor quality by OSBA were tested (The ratios of embryos developed from PN 1-cell stage to hatched blastocyst; 25% vs. 70%). In the medium with good quality, sperm binding ability was significantly increased (P ＜ 0.05). The ratio of oocytes bound with one sperm at least was 66% and 90% in the medium with poor and good quality, respectively. Conclusively, It was possible to test IVF medium condition rapidly and easily by OSBA.

• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.367-375
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• 2010

The bacteriological and physiochemical analysis of sea water and sediments in Tongyeong harbor was conducted to evaluate sanitary conditions. The samples were collected at 8 stations established once a month from June, 2008 to May, 2009. During the study period, the range of temperature was from 6.7 to $25.2^{\circ}C$, transparency ranged from 1.2 to 2.6 m, chemical oxygen demand ranged from 1.90 to 2.92 mg/L, dissolved oxygen ranged from 6.2 to 10.5 mg/L, dissolved nitrogen ranged from 0.052 to 0.098 mg/L, phosphate ranged from 0.044 to 0.065 mg/L, respectively. Seafood, if eaten raw, carries the risk of food poisoning. Seafood poisoning is often cause by pathogenic microorganism originating from fecal contamination, such as Salmonella sp., Shigella sp. and norovirus. Fecal coliforms are an important indicator of fecal contamination. Therefore, data on fecal coliform are very important for evaluating the safety of fisheries in coastal areas. So, we investigated the sanitary indicate bacteria. The coliform group and fecal coliform MPN's of sea water in Tongyeong harbor were ranged from < 1.8~22,000/100 mL (GM 164.9 MPN/100 mL) and < 1.8~7,900 MPN/100 mL (GM 33.7 MPN/100 mL), respectively. Total coliform were detected 97.0% in 96 of samples and 68.9% of total coliforms were fecal coliforms. These results similar to another seawater detection ratio of total coloforms and fecal coliforms. The Vibrios was isolated and identified with VITEK system. Four hundred eighty strains that were obtained from sea water samples in Tongyeong harbor Detection ratio Vibrio alginolyticus, 34.2%, Vibrio parahaemolyticus, 13.8%, Vibrio vulnificus 10.0%, and V. mimicus 12.5% respectively. Vibrio cholerae O1, was not detected. During the study period, the ranges of water content, ignition loss, COD, and acid volatile sulfates in sediments in Tongyeoung harbor were 41.0~57.4%, 7.8~10.5%, 6.51~9.30 mg/g, 0.04~0.09 mg/g, respectively. Heavy metals in sediment of Tongyeoung harbor were Cd, $0.10{\pm}0.05$; Cu, $4.79{\pm}8.20$; As, $1.95{\pm}0.17$; Hg, $0.10{\pm}0.07$; $Cr^{6+}$, $0.34{\pm}0.12$; Zn, $125.33{\pm}16.40$; Ni, $16.43{\pm}1.93$ mg/kg.