• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.291-300
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• 2018

Background: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215. Methods: Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at $-80^{\circ}C$, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at $4^{\circ}C$, and analyzed for their stability and in vitro quality. Results: No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at $4^{\circ}C$ for 7 days were acceptable to the American Blood Bank Association's standards and European standards. Conclusion: RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.

• Journal of Environmental Impact Assessment
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• v.28 no.3
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• pp.332-345
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• 2019

The purpose of the study is to evaluate the treatability of contaminated groundwater with TPH and (or) $Cr^{6+}$. Laboratory scale tests were performed for oil/water separation, dissolved air flotation (DAF), coagulation and precipitation, and filtration with sand and activated carbon respectively. Two times of oil/water separation tests for total 40 minutes of separation or separating time shows 90.2 % of TPH removal rate. In case of DAF test for high TPH sample, the TPH removal rates were not varied significantly by the variation of microbubble size. However, tests for low TPH samples show that TPH removal rate increases as microbubbles are smaller. When coagulant was added to sample for DAF test, TPH removal rate was increased 12.3 %. SS removal rate by DAF was 97.9 % at $16-40{\mu}m$ and it was increased as the size of microbubble is reduced. Tests for coagulation and precipitation were performed to evaluate the removal of $Cr^{6+}$ in groundwater. The increase of $FeSO_4$ dosage increased $Cr^{6+}$ removal rate in the coagulation and precipitation process. As the amount of activated carbon in the filter media increased TPH removal rate in the filtration process. SS removal rate by the filtration was 96.7 % similar to the results of DAF process tests. The filtration process treats TPH and SS. Best design parameters are determined as the size of sand is $425-850{\mu}m$ and the ratio of activated carbon and sand is 50:50.

• Korean Journal of Remote Sensing
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• v.32 no.3
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• pp.221-234
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• 2016

In Korea, most industrial parks and major cities are located in coastal areas, which results in serious environmental problems in both coastal land and ocean. In order to effectively manage such problems especially in coastal ocean, water quality should be monitored. As there are many factors that influence water quality, the Korean Government proposed an integrated Water Quality Index (WQI) based on in situmeasurements of ocean parameters(bottom dissolved oxygen, chlorophyll-a concentration, secchi disk depth, dissolved inorganic nitrogen, and dissolved inorganic phosphorus) by ocean division identified based on their ecological characteristics. Field-measured WQI, however, does not provide spatial continuity over vast areas. Satellite remote sensing can be an alternative for identifying WQI for surface water. In this study, two schemes were examined to estimate coastal WQI around Korea peninsula using in situ measurements data and Geostationary Ocean Color Imager (GOCI) satellite imagery from 2011 to 2013 based on machine learning approaches. Scheme 1 calculates WQI using estimated water quality-related factors using GOCI reflectance data, and scheme 2 estimates WQI using GOCI band reflectance data and basic products(chlorophyll-a, suspended sediment, colored dissolved organic matter). Three machine learning approaches including Random Forest (RF), Support Vector Regression (SVR), and a modified regression tree(Cubist) were used. Results show that estimation of secchi disk depth produced the highest accuracy among the ocean parameters, and RF performed best regardless of water quality-related factors. However, the accuracy of WQI from scheme 1 was lower than that from scheme 2 due to the estimation errors inherent from water quality-related factors and the uncertainty of bottom dissolved oxygen. In overall, scheme 2 appears more appropriate for estimating WQI for surface water in coastal areas and chlorophyll-a concentration was identified the most contributing factor to the estimation of WQI.

• Korean Journal of Environmental Agriculture
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• v.32 no.2
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• pp.166-170
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• 2013

BACKGROUND: Persimmon growers have often tried various regimens of K fertilization to improve fruit quality. This experiment was conducted to determine the effects of K rates on concentration of inorganic elements in different tree organs and on fruit characteristics. METHODS AND RESULTS: Six-year-old non-astringent 'Fuyu' persimmons, grown in 50-L pots, were used. Total K amounts of 0 (no-application), 12, 25, 37, and 66 g were fertigated to a pot with KCl solution at 3-to 4-day intervals from July to September. The 0 K trees received no K fertilizer for the two previous years. Leaves, fruits, and shoots were sampled in November. K concentrations in leaves and shoots increased significantly by increasing K rate; leaf K, 0.49% for the 0 K, increased to 3.09% for the 37 g and 3.11% for the 66 g trees. Fruit K was notably lower for the 0 K, but there were no significant differences among the trees as long as they were supplied with more than 12-g K. In the trees with 0 K, leaf necrosis in the margin was apparent in June and the symptom progressed toward the midrib. Some leaves scorch-rolled from the margin in August. The greatest effect of K rates was on fruit size; it significantly increased to 181 g for the 12 g, 203 g for the 37 g, and 206 g for the 66 g compared with 150 g for the 0 K trees. However, K rates did not affect firmness and soluble solids of the fruits. The fruits of the 0 K trees were characterized by better coloration. CONCLUSION(S): The K-rate effect on inorganic elements depended on tree organs and fruit size was the major parameter to be affected by the K rates.

• Journal of the Korean Society of Marine Environment & Safety
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• v.23 no.5
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• pp.513-523
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• 2017

We evaluated the viability of phytoplankton along the salinity gradient in the flood and ebb tides of spring tide of February and the ebb tide of neap tide of March 2017 in the Seomjin River Estuary. Additional laboratory experiments were also conducted to determine the reason of the pH changes along the salinity gradient using the field natural sample in February. In field, saltwater was well mixed at downstream vertically and the salinity gradient was horizontally appeared toward upstream of freshwater zone. There were strong negative correlations between salinity and nutrient (nitrate + nitrite R=0.99, p<0.001, and silicate R=0.98, p<0.001), implying that those two nutrients of freshwater origin were gradually diluted with mixing the saltwater. On the other hands, relatively high phosphate concentration was kept in the stations of saltwater over 15 psu, indicating that it was caused by resuspended sediments of Gwangyang Bay and downstream by tidal water mixing.Among phytoplankton community structure in winter, Eucampia zodiacus have occupied to be c.a. 70 % in the most stations. Based on the field survey results for survivability of phytoplankton by phytoPAM instrument, there was positive correlations between salinity and chlorophyll a (R=0.82, p<0.001) and, salinity and active chlorophyll a (R=0.80, p<0.001), implying that the dominant marine diatom species may have significantly damaged in low salinity conditions of upstream. Also, maximum mortality rate of phytoplankton caused by low salinity shock was appered to be 75% in the upstream station. In particular, the pH in spring tides of February had tended to increase with high phytoplankton accmulated stations, suggesting that it was related with absorption of $CO_2$ by the photosynthesis of dominant diatom. In laboratory experiments, phytoplankton mass-mortality caused by low salinity shock was also occurred, which is confirmed with reducing the photosynthetic electron transport activity. Following the phytoplankton mass-mortality, bacteria abundance was significantly increased in 24 hours. As a result, the mass-proliferating bacteria can produce the $CO_2$ in the process of biodegradation of diatoms, which can lead to pH decrease. Therefore, marine phytoplankton species was greatly damaged in freshwater mixing area, depending on along the salinity gradient that was considered to be an important role in elevating and reducing of pH in Seomjin River Estuary.

• Journal of Yeungnam Medical Science
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• v.11 no.2
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• pp.293-302
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• 1994

The objective of this study was to establish a good methodology to isolate single smooth muscle cells that are alive and respond properly to pharmacological agents. Canine urinary bladders were employed as the source of single cells, and acetylcholine, atropine and imipramine were used as indicators of pharmacological responsiveness. Imipramine, an antidepressant drug exhibited the anticholinergic and calcium antagonizing properties on rat detrusor muscle. To establish a control value for a further experiment to elucidate the mechanism of action of imipramine on detrusor muscle, we measured the concentration-response of single cells to acetylcholine in the presesnce of imipramine by length of the cells and compared the result with the response in the presence of atropine. Tiny chops of smooth muscle taken from anesthetized canine urinary bladder were incubated in collagenase solution at $36^{\circ}C$ for 17-20 minutes. The collagenase solution included collagenase 1.2 mg/ml, soybean tryspin inhibitor 0.08 mg/ml, bovine serum albumin 2% in 10 ml Krebs-Henseleit buffer solution aerated with a consistent breeze of 95/5% $O_2/CO_2$, to maintain the pH at 7.4. After washing with plain K-H solution on 450 mesh, cells were dissociated from the digested tissue for 12-15 minutes. Cell suspension was transfered in 5 ml test tubes and acetylcholine was added for the final concentration to be $10^{-14}M{\sim}10^{-9}M$. To find the optimal time to fix the cells to determine the contractile responses, 1% acrolein was added 5, 10, 20, 30, 60 and 120 seconds after the administration of ACh. The length of cells fixed by acrolein were measured by microscaler via CCTV camera on phaes-contrast microscope. The average length of 50 cells from a slide glass was taken as the value of a sample at the very concentration point. Single cells were isolated from canine detrusor. The length of untreated cells varied from 82 ${\mu}m$ to 94 ${\mu}m$. The maximal response to actylcholine $10^{-9}M$ was accomplished within 5 seconds of exposure, and the shortening was $19{\pm}3$%. Atropine reduced the contraction of the cells concentration-dependently. Imipramine which exerts a cholinergic blocking action on some smooth muscles also reduced the contraction concentration-dependently and by a similar pattern as atropine. These findings document that imipramine may exerts a cholinergic blocking activity in the single smooth muscle cells isolated from canine urinary bladder.

• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.166-173
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• 2006

This research has been performed to clarify the relationship between hydrogeochemistry and bacterial community structure in groundwater contaminated with landfill leachate. We collected and analyzed samples from 5 sites such as leachate (KSG1-12), treated leachate (KSG1-16), two contaminated groundwaters (KSG1-07 and KSG1-08) and non-contaminated groundwater (KSG1-13). pH was 8.83, 8.04, 6.87, 6.87 and 6.53 in order; redox potential (Eh) 108, 202, 47, 200 and 154 mV; electric conductivity (EC) 3710, 894, 1223, 559 and 169.9 $\mu$S/cm; suspended solids (SS) 86.45, 13.74, 4.18, 0.24 and 11.91 mg/L. In KSG01-12, the ion concentrations were higher especially in $Cl^-$ and $HCO_3^-$ than other sites. The concentrations of Fe, Mn and $SO_4^{2-}$ were higher In KSG1-07 than in KSG1-08, and vise versa in $NO_3^{2-}$. In the comparison of DGGE fingerprint patterns, the similarity was highest between KSG1-13 and KSG1-16 (57.2%), probably due to common properties like low or none contaminant concentrations. Otherwise KSG1-08 showed lowest similarities with KSG1-13 (25.8%) and KSG1-12 (27.6%), maybe because of the degree of contamination. The most dominant bacterial species in each site were involved in $\alpha$-Proteobacteria (55.6%) in KSG1-12, $\gamma$-Proteobacteria (50.0%) in KSG1-16, $\beta$-Proteobacteria (66.7%) in KSG1-07, $\gamma$-Proteobacteria (54.5%) in KSG1-08 and $\beta$-Proteobacteria (36.4%) in KSG1-13. These results indicate that the microbial community structure might be changed according to the flow of leachate in grounderwater, implying changes in concentrations of pollutants, available electron accepters and/or other environmental conditions.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.129-138
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• 2012

We investigated an ecological impact of drought simulated by discharge depletion on the water quality and benthic diatom community in the indoor experimental channel. As artificial substrates slide-glass was installed in acrylic channel for 16 days. Channels were supplied continuously with eutrophic lake water with a discharge rate of 6 L $min^{-1}$ in duplication during the colonized period. And then during the discharge depletion period, three discharge rates were provided: NDF (No depletion of flow rate (Control): 6 L $min^{-1}$), LDF (Low depletion of flow rate: 3 L $min^{-1}$) and HDF (High depletion of flow rate: 1 L $min^{-1}$). Environmental factors in the water, such as suspended solid, Chl-$a$ and nutrients concentration, were measured with periphytic algae including AFDM (ash free dry matter), Chl-$a$ concentration and cell density at 1-day intervals. Light intensity increased significantly with discharge depletion (F=229.5, p= 0.000). $NH_4$-N concentration was highest at HDF. Suspended solid in outflowing water decreased at HDF (88%), LDF (97%) and NDF (99%), compared to inflowing water (100 %). Chl-$a$ in substrates increased more than two times at LDF and HDF than NDF (F= 8.399, p=0.001). Also AFDM and benthic diatom density increased significantly at LDF and HDF than NDF (F=9.390, p=0.001; F=6.088, p=0.007). In all experimental groups, $Aulacoseira$ $ambigua$, $Achnanthes$ $minutissima$ and $Aulacoseira$ $granulata$ were dominant species accounting for greater than 10% of benthic diatom density. The most dominant species, $A.$ $ambigua$ was highest at LDF, followed by HDF and NDF (F=8.551, p=0.001). In conclusion, the effect of drought simulated by discharge depletion in an artificial stream ecosystem caused significant changes on water quality and benthic diatom biomass. This result provides a useful data to understand the effect of draught on stream ecosystem in situ.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.161-169
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• 2003

Seawater and sediment quality analysed was calculated to examinate the present environmental characteristics and pollution load was also calculated to evaluate the effect of farming area on the coastal environment. The measurements for seawater quality demonstrate the coastal environment has relatively eutrophicated with significantly decreased DO (0.2-8.5 mg/l) and elevated COD (9.6-31.2 mg/l) in summer. It was also evident that the water quality in Jindong Bay has been influenced by residues tide from Masan Bay with high metal concentration in August of 2002. Annual total pollution load (land and farm-driven) was estimated at 37,316 ton (SS) /yr: 9,809 ton/yr (26.3%) of land-driven load, 23,576 ton/yr (63.2%) of coastal sedimentation and 3,932 ton/yr (10.5%) of feces of cultural organisms. When all ark shell seedling farms are permitted species conversion to ascidian farm, the pollution load would increase by 196%, which may be another source for accelerating the eutrophication of the environment in Jindong Bay.

• Korean Journal of Environmental Agriculture
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• v.23 no.1
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• pp.59-67
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• 2004

The Shihwa constructed wetland was established for the treatment of severely polluted water from Banwoul, Donghwa and Samhwa streams. This study was focused on investigating dynamics of phytoplankton communities in open waters and periphytic algae on reed (Phragmites communis) planting area at 5 stations from October 2001 to July 2002. The concentration of T-N and T-P of inlet stations from the streams were decreased by flowed through the wetland. However, the TN/TP ratios at all stations were shown as a little over 16 indicating that the T-P will play some role as a limitation factor. Phytoplankton communities were identified as a total 413 taxa which were composed of 375 species, 21 varieties, 2 forma and 15 unidentified species. Standing crops of phytoplankton communities and chlorophyll-a concentrations ranged from $330{\sim}36,420\;cells/mL$ and $2.5{\sim}170.7\;{\mu}g/L$ respectively, and showed the decreasing tendency after flowing through the wetland at almost all stations. Dominant species were 14 taxa at all stations which were Euglena oblonga and Synura spinosa etc. Periphytic algae on the reed were a total 329 taxa which were composed of 295 species, 13 varieties, 3 forma and 17 unidentified species. The species numbers were recorded in order of Chlorophyceae-Bascillariophyceae-Cyanophyceae-Euglenophyceae-Chrysophyceae. The relative abundance (%) was showed a seasonal variation from Cyanophyceae to Bascillariophyceae and to Chlorophyceae. Chlorophyll-a concentrations, ie. the biomass of periphytic algae were ranged from $5.5{\sim}363.8\;mg/m^2$. Dominant species were 16 taxa which were Lyngbya angusta of Cyanophyceae in the early investigation, and were changed to Stigeoclonium lubricum of Chlorophyceae, and Nitzschia plea of Bacillariophyceae etc. in the late. Species number, standing crops and chlorophyll-a concentrations of phytoplankton and perphytic algae were shown higher values at the inlet stations than the stations after flowing through the wetland.