• Title/Summary/Keyword: 복합학

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Fermentative characteristics of yogurt using lactic acid bacteria isolated from Korean traditional fermented food (전통 발효 식품에서 분리한 유산균을 이용한 yogurt 발효특성)

  • Park, Na-Young;Lee, Shin-Ho
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.707-713
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    • 2017
  • The objective of this study was to select yogurt starter from Korean traditional fermented foods. The 2 strains (KM24, KM32) among 50 strains of isolated lactic acid bacteria selected as starter based on milk clotting ability, antimicrobial activity against various pathogens, tolerance in artificial gastric and bile juice and growth in 10 % skimmed milk. The strains were identified as Lacobacillus plantarum (KM32) and Pediococcus pentosacesus (KM24) by 16S rRNA gene sequencing. Viable cell number of yogurt fermented with mixed strains (KM24 and KM32) was 9.66 log CFU/mL after fermentation for 48 h and maintained $10^9CFU/mL$ during fermentation for 72 h at $37^{\circ}C$. The pH and titratable acidity of mixed cultured yogurt were 4.25% and 0.83% after fermentation for 48 h at $37^{\circ}C$, respectively. The physico-chemical characteristics of mixed cultured yogurt after fermentation for 48 h were $38.45{\mu}g/mL$ (polyphenol content), 48.57% (DPPH radical scavenging activity) and 465.40 cp (viscosity), respectively. The mixed cultured yogurt maintained $10^9CFU/mL$ of lactic acid bacteria during storage 10 days at $4^{\circ}C$. The viable cell number of yogurt prepared with mixed culture(KM32+KM24) maintained higher and than that of control (L. casei) during storage. These results indicated the potential use of selected strains (KM32+KM24) isolated from kimchi as a yogurt starter with strong acid tolerance and probiotics properties.

Study on Oxidation and Coercivity of Nd2Fe14B Compound Crystal (Nd2Fe14B 화합물 결정의 산화 및 보자력에 관한 연구)

  • Kwon, H.W.;Yu, J.H.
    • Journal of the Korean Magnetics Society
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    • v.22 no.3
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    • pp.85-90
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    • 2012
  • Oxidation of the $Nd_2Fe_{14}B$ compound crystal and its effect on the coercivity of the fine $Nd_2Fe_{14}B$ crystal particles were investigated. Oxidation kinetics of the $Nd_2Fe_{14}B$ compound crystal was investigated using an excessively grown $Nd_2Fe_{14}B$ grains in the $Nd_{15}Fe_{77}B_8$ alloy ingot. Oxidation of the $Nd_2Fe_{14}B$ compound crystal occurred by dissociation of the phase into multi-phase mixture of ${\alpha}$-Fe, $Fe_3B$, and Nd oxides. Oxidation rate of the $Nd_2Fe_{14}B$ compound crystal showed no dependence on the crystallographic direction. The oxidation reaction was modeled according to simple linear relationship. Activation energy for the oxidation of $Nd_2Fe_{14}B$ compound crystal was calculated to be approximately 26.8 kJ/mol. Fine $Nd_2Fe_{14}B$ crystal particles in near single domain size was prepared by ball milling of the HDDR-treated $Nd_{15}Fe_{77}B_8$ alloy, and these particles were used for investigating the effect of oxidation on the coercvity. The near single domain size $Nd_2Fe_{14}B$ crystal particles (${\fallingdotseq}0.3\;{\mu}m$) had high coercivity over 9 kOe. However, the coercivity was radically reduced as the temperature increased in air (<2 kOe at $200^{\circ}C$). This radical coercivity reduction was attributed to the soft magnetic phases, ${\alpha}$-Fe and $Fe_3B$, which were formed on the surface of the fine particles due to the oxidation.

Mechanism of Growth Inhibition in Herbicide-Resistant Transgenic Rice Overexpressing Protoporphyrinogen Oxidase (Protox) Gene (Protoporphyrinogen Oxidase (Protox) 유전자 과다발현 제초제 저항성 형질전환 벼의 생육저해 기작)

  • Kuk, Yong-In;Shin, Ji-San;Yun, Young-Beom;Kwon, Oh-Do
    • Korean Journal of Weed Science
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    • v.30 no.2
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    • pp.122-134
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    • 2010
  • We investigated the levels of resistance and accumulation of terapyrroles, reactive oxygen species, lipid peroxidation, and antioxidative enzymes for reasons of growth reduction in herbicide-transgenic rice overexpressing Myxococcus xanthus, Arabidopsis thaliana, and human protoporphyrinogen oxidase (Protox) genes. The transgenic rice overexpressing M. xanthus (MX, MX1, PX), A. thaliana (AP31, AP36, AP37), and human (H45, H48, H49) Protox genes showed 43~65, 41~72 and 17~70-fold more resistance to oxyfluorfen, respectively, than the wild type. Among transgenic rice lines overexpressing Protox genes, several lines showed normal growth compared with the wild type, but several lines showed in reduction of plant height and shoot fresh weight under different light conditions. However, reduction of plant height of AP37 was much higher than other lines for the experimental period. On the other hand, the reduction of plant height and shoot fresh weight in the transgenic rice was higher in high light condition than in low light condition. Enhanced levels of Proto IX were observed in transgenic lines AP31, AP37, and H48 at 7 days after seeding (DAS) and transgenic lines PX, AP37, and H48 at 14 DAS relative to wild type. There were no differences in Mg-Proto IX of transgenic lines except for H41 and H48 and Mg-Proto IX monomethyl ester of transgenic lines except for MX, MX1, and PX. Although accumulation of tetrapyrrole intermediates was observed in transgenic lines, their tetrapyrrole accumulation levels were not enough to inhibit growth of transgenic rice. There were no differences in reactive oxygen species, MDA, ALA synthesizing capacity, and chlorophyll between transgenic lines and wild type indicating that accumulated tetrapyrrole intermediate were apparently not high enough to inhibit growth of transgenic rice. Therefore, the growth reduction in certain transgenic lines may not be caused by a single factor such as Proto IX, but by interaction of many other factors.

A Study on Comparative Analysis of Socio-economic Impact Assessment Methods on Climate Change and Necessity of Application for Water Management (기후변화 대응을 위한 발전소 온배수 활용 양식업 경제성 분석)

  • Lee, Sangsin;Kim, Shang Moon;Um, Gi Jeung
    • Journal of Korean Society of societal Security
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    • v.4 no.2
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    • pp.73-78
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    • 2011
  • In order to resolve the problem of change in global climate which is worsening as days go by and to preemptively cope with strengthened restriction on carbon emission, the government enacted 'Framework Act on Low Carbon Green Growth' in 2010 and selected green technology and green industry as new national growth engines. For this reason, the necessity to use the un-utilized waste heat across the whole industrial system has become an issue, and studies on and applications of recycling in the agricultural and fishery fields such as cultivation of tropical crops and flatfishes by utilizing the waste heat and thermal effluent generated by large industrial complexes including power plants are being actively carried out. In this study, we looked into the domestic and overseas examples of having utilized waste heat abandoned in the form of power plant thermal effluent, and carried out economic efficiency evaluation of sturgeon aquaculture utilizing thermal effluent of Yeongwol LNG Combined Cycle Power Plant in Gangwon-do. In this analysis, we analyzed the economic efficiency of a model business plan divided into three steps, starting from a small scale in order to minimize the investment risk and financial burden, which is then gradually expanded. The business operation period was assumed to be 10 years (2012~2021), and the NVP (Net Present Value) and economic efficiency (B/C) for the operation period (10 years) were estimated for different loan size by dividing the size of external loan by stage into 80% and 40% based on the basic statistics secured through a site survey. Through the result of analysis, we can see that reducing the size of the external loan is an important factor in securing greater economic efficiency as, while the B/C is 1.79 in the case the external loan is 80% of the total investment, it is presumed to be improved to 1.81 when the loan is 40%. As the findings of this study showed that the economic efficiency of sturgeon aquaculture utilizing thermal effluent of power plant can be secured, it is presumed that regional development project items with high added value can be derived though this, and, in addition, this study will greatly contribute to reinforcement of the capability of local governments to cope with climate change.

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Effects of Local Climatic Conditions on the Early Growth in Korean White Pine (Pinus koraiensis Sieb. et Zucc.) Stands -Relation between Annual Increment and Local Climatic Conditions- (지역별 잣나무 초기생장에 미치는 미기후의 영향 - 연년생장과 미기후와의 관계-)

  • Chon Sang- Keun;Shin Man Yong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.1
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    • pp.41-51
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    • 1999
  • This study was conducted to investigate the effects of local climatic conditions on the annual increment of Korean white pine planted in Gapyung and Yaungdong. For this, stand variables such as mean DBH, mean height, basal area per hectare, and volume per hectare by stand age were measured and summarized for each locality. Based on these statistics, annual increments for 8 years from stand age 10 to 18 were calculated for each of stand variables. A topoclimatological technique which makes use of empirical relationships between the topography and the weather in study sites was applied to produce normal estimates of monthly mean, maximum, minimum temperatures, relative humidity, precipitation, and hours of sunshine. Then, the yearly climatic variables from 1990 to 1997 for each study site were derived from the spatial interpolation procedures based on inverse- distance weighting of the observed deviation from the climatic normals at the nearest 11 standard weather stations. From these estimates, 17 weather variables such as warmth index, coldness index, index of aridity etc., which affect the tree growth, were computed on yearly base for each locality. The deviations of measured annual increments from the expected annual increments for 8 years based on yield table of Korean white pine were then correlated with and regressed on the yearly weather variables to examine effects of local climatic conditions on the growth. Gapyung area provides better conditions for the growth of Korean white pine in the early stage than Youngdong area. This indicates that the conditions such as low temperature, high relative humidity, and large amount of precipitation provide favor environment for the early growth of Korean white pine. A ccording to the correlation and regression an analysis using local climatic conditions and annual increments, the growth pattern of Gapyung area corresponds to this tendency. However, it was found that the relationship between annual increments and local climatic conditions in Youngdong area shows different tendency from Gapyung. These results mean that the yearly growth pattern could not sufficiently be explained by climatic conditions with high variance in yearly weather variables. In addition, the poor growth in Youngdong area might not only be affected by climatic conditions, but also by other environmental factors such as site quality.

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Fatigue fracture of different dental implant system under cyclic loading (반복하중에 따른 수종 임플란트의 피로파절에 관한 연구)

  • Park, Won-Ju;Cho, In-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.4
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    • pp.424-434
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    • 2009
  • Statement of problem: Problems such as loosening and fractures of retained screws and fracture of implant fixture have been frequently reported in implant prosthesis. Purpose: Implant has weak mechanical properties against lateral loading compared to vertical occlusal loading, and therefore, stress analysis of implant fixture depending on its material and geometric features is needed. Material and methods: Total 28 of external hexed implants were divided into 7 of 4 groups; Group A (3i, FULL $OSSEOTITE^{(R)}$Implant), Group B (Nobelbiocare, $Br{\aa}nemark$ $System^{(R)}$Mk III Groovy RP), Group C (Neobiotec, $SinusQuick^{TM}$ EB), Group D (Osstem, US-II). The type III gold alloy prostheses were fabricated using adequate UCLA gold abutments. Fixture, abutment screw, and abutment were connected and cross-sectioned vertically. Hardness test was conducted using MXT-$\alpha$. For fatigue fracture test, with MTS 810, the specimens were loaded to the extent of 60-600 N until fracture occurred. The fracture pattern of abutment screw and fixture was observed under scanning electron microscope. A comparative study of stress distribution and fracture area of abutment screw and fixture was carried out through finite element analysis Results: 1. In Vicker's hardness test of abutment screw, the highest value was measured in group A and lowest value was measured in group D. 2. In all implant groups, implant fixture fractures occurred mainly at the 3-4th fixture thread valley where tensile stress was concentrated. When the fatigue life was compared, significant difference was found between the group A, B, C and D (P<.05). 3. The fracture patterns of group B and group D showed complex failure type, a fracture behavior including transverse and longitudinal failure patterns in both fixture and abutment screw. In Group A and C, however, the transverse failure of fixture was only observed. 4. The finite element analysis infers that a fatigue crack started at the fixture surface. Conclusion: The maximum tensile stress was found in the implant fixture at the level of cortical bone. The fatigue fracture occurred when the dead space of implant fixture coincides with jig surface where the maximum tensile stress was generated. To increase implant durability, prevention of surrounding bone resorption is important. However, if the bone resorption progresses to the level of dead space, the frequency of implant fracture would increase. Thus, proper management is needed.

COMPARATIVE STUDY ON FLUORIDE RELEASE AND RE-UPTAKE CAPACITY OF SEVERAL FLUORIDE-RELEASING RESTORATIVE MATERIALS (수종의 수복재의 불소 적용법에 따른 불소 유리에 관한 비교 연구)

  • Lee, Yeon-Ho;Yoo, Seung-Hoon;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.1
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    • pp.25-34
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    • 2006
  • Fluoride released from dental restorative materials effectively declines the incidence and activity of dental caries and inhibits tooth demineralization. This study investigated the fluoride release and uptake characteristics of one composite resin $(Z-250^{TM})$ three glass ionomer-based restorative material ($Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$, Fuji IX GP $Fast^{(R)}$) Forty discs(6mm diameter and 1mm height) were prepared for each material. Each disc was immersed in 5ml of distilled water within polyethylene vial and stored at $37^{\circ}C$. The distilled water was changed every 24 hours and the release of fluoride was measured for 31 days. At the end of this period, each specimen was subjected to one of four treatments : (A) no fluoride treatment (control), (B) application of a fluoride dentifrice (500ppm) for three minutes three times; (C) application of the 1.23% acidulated phosphate fluoride(APF) foam for one minute once, (D) the same regimen as (B), plus application of the APF foam for one minute once. Then, all samples were reassessed for an additional 7 days. For all samples, the greatest fluoride release was observed after the first day of the study but diminished with time. On the 7th day of the study, fluoride release level was stabilized. Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ released higher amount of fluoride than other materials ; however, no statistically significant difference was found from Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$. The amount of fluoride of $Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ was increased after fluoride treatment, and diminished with time.

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DIRECT PULP CAPPING WITH BONDING RESIN (접착용 레진을 이용한 유치의 직접 치수복조술에 관한 연구)

  • Cho, Hae-Sung;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.2
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    • pp.165-172
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    • 2006
  • A direct pulp capping with different medicaments has been attempted for a long time. The most commonly used among those is the calcium hydroxide. In primary teeth, however, a success rate of direct pulp capping with calcium hydroxide has been reported to be lower than that of pulpotomy. The disappointing results of calcium hydroxide have prompted the search for other capping materials. Lately, several researchers suggested an application of adhesive resin-based composite systems as a capping material. They claimed that when an exposed vital pulp is capped directly with bonding resin, the pulp tissue is free of inflammation or necrosis without clinical symptoms. The aim of this study was to compare short-term effects of the bonding resin which was applied on the mechanically exposed vital pulp tissue and those of direct pulp capping with calcium hydroxide. The second objective was to compare success rates of the primary teeth which already underwent physiologic root resorption and those of the teeth which had not undergone physiologic root resorption yet, in each capping material groups. The vital, healthy pulp of forty-one primary teeth were exposed mechanically during a cavity preparation. They were divided into two groups: Group 1(n=21) underwent capping with bonding resin, and group 2(n=20) underwent capping with calcium hydroxide. Then these two groups were subdivided into two groups in each : the teeth which show physiologic root resorption and the teeth without root resorption. All of the sample teeth were restored with composite resin. Clinical evaluations such as percussion test, ice test, EPT, were recorded and also before- and after- standard x-ray films were compared and evaluated to decide whether the case was successful or not. Evaluation was performed at least 3 months after the capping materials. The results were as follows 1. There was no difference in success rate between group 1 and group 2. 2. Success rate of the teeth with physiologic root resorption was higher than that of the teeth without physiologic root resorption in group 1 and group 2. 3. There was no difference in success rate between anterior teeth and posterior teeth.

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A STUDY OF THE EFFECT OF CHEMO-MECHANICAL CARIES REMOVAL SYSTEM ON THE REMOVAL OF CARIOUS DENTIN AND RESIN ADHESION TO DENTIN (화학제재를 이용한 우식상아질 제거효과 및 레진과의 결합강도에 관한 연구)

  • Kang, Dug-Il;Park, In-Chon;Lee, Nan-Young;Lee, Sang-Ho;Lee, Chang-Seop
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.4
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    • pp.581-592
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    • 2003
  • The aim of this study was to evaluate the effect of chemo-mechanical caries removal system($Carisolv^{TM}$, Medi Team, Sweden) for resin adhesion to carious primary and permanent dentin compared with conventional drilling method. The buccal surface of 92 primary molars and 92 permanent molars were used. Exposed dentins were occurred artificial caries. 32 tooth of primary molars and 32 tooth of permanent molars were prepared to observe treated dentin surface with $Carisolv^{TM}$ and conventional drilling method by SEM. Other tooth were prepared to measure resin-dentin shear bonding strength according to caries removal methods and dentin adhesive system. Two adhesive systems and a composite resin were used; single bonding agent(Scotchbond Multi-Purpose Plus, 3M) and self-etching bonding system(Prompt L-pop, 3M ESPE), and a composite resin (Z-250, 3M). The results were as follows : 1. The removal effect of carious dentin on $Carisolv^{TM}$ was stronger on the primary dentin than that to permanent dentin, and dentin surface became rougher with treated $Carisolv^{TM}$ than drilling method. 2. Acid-etched dentin surfaces were showed smoothening without smear layer. 3. In specimen applied single bonding system hybrid layer and adhesive layer were $2-4{\mu}m$ and $10-15{\mu}m$ in thickness, whereas self-etching bonding system were showed only thin hybrid layer($1-2{\mu}m$). 4. The shear bonding strength of group applied single bonding agent was higher than that applied self-etching priming system(P<0.05). 5. The shear bonding strength of group applied $Carisolv^{TM}$ and self-etching priming system were slightly higher than that applied conventional drilling method and self-etching priming system(P>0.05).

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EFFECT OF UNREACTED RESIN MONOMES ON THE ATIVITY OF CARIOGENIC BACTERIA (미반응 레진단량체가 우식유발성 세균의 활성에 미치는 영향)

  • Park, Seung-Kyu;Kim, Hwa-Sook;You, So-Young;Han, Jin-Ju;Kook, Joong-Ki;Lee, Nan-Young;Lee, Sang-Ho;Lee, Chang-Seop
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.4
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    • pp.684-695
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    • 2003
  • The aim of this study was to investigate the effect of composite resin components on proliferation and glucan synthesis by cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. Light curing pit and fissure sealant was chosen for evluation. Specimens were eluted in deionized water for 10 minutes, 1, 12, and 24 hours. Extracts of specimens were diluted into 1/2, 1/4, and 1/8 with addition of BHI broth and BHI-YS. Bacteria were cultured in media included eluted components, and measured optical density($A_{600}$). The following results were obtained 1. 1/4 concentration of elutes for 10 minutes significantly inhibited the proliferation of S. mutans, whereas 1/2, 1/8 concentration of elutes stimulated it. Also, exacts, especially 1/2, 1/4 concentration, for 1 hours stimulated it. But exacts for 12, 24 hours had not effects on the proliferation of S. mutans. 2. 1/4 concentration of elutes for 10 minutes inhibited growth of S. sobrinus, whereas extracts for 1, 12, 24 hours had not effects on the proliferation of S. sobrinuss. 3. Extracts from composite resin stimulated total growth of S. mutans more than growth control group, where as inhibited it of S. sobrinus. 4. Extracts from composite resin, especially 1/4 concentration of it for 10 minutes increased the formation of water insoluble glucan of S. mutans. But elutes for 1, 12, 24 hours, and 1/8 concentration of it for 10 minutes inhibited it. 5. Except 1/4 concentration of elutes for 10 minutes, extracts decreased the formation of water insoluble glucan of S. sobrinus. 6. Total amount of formated glucan was 3-fold higher in S. mutans than in S. sobrinus.

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