• Journal of Life Science
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• v.13 no.3
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• pp.298-307
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• 2003

To figure out the conserved genes and newly added genes at each phylogenetic level of Archaea, COG (clusters of orthologous groups of proteins) algorithm was applied. The number of conserved genes within 9 species of Archaea was 340 and that of 8 species of Euryarchaeota was 388. Many of conserved 265 COGs, which are specific to Archaea and absent in Bacteria and S. cerevisiae, were concerned with 'information storage and processing' (94 COG, 35.5%) and 'metabolism' (82 COG, 30.9%). COGs related to these functions were assumed as highly conserved and permit peculiar life form to Archaea. It seemed that there was some difference in 'nucleotide transport and metabolism' and there was little difference in 'information storage and processing' between Euryarchaeota and Crenarchaeota. Marine-origin Euryarchaeota showed different conserved COGs with terrestrial Euryarchaeota. Conserved COGs, related to carbohydrate transport and metabolism and others, were different between marine- and terrestrial-origin Euryarchaeota. Hence it was assumed that their physiology might be different. This study may help to understand the origin and conserved genes at each phylogenetic level of marine-origin Euryarchaeota and may help in the mining of useful genes in marine Archaea as Manco et al. (Arch. Biochem. Biophy. 373, 182 (2000)).

• Journal of the Korean Society for Library and Information Science
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• v.49 no.2
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• pp.111-134
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• 2015

This research was initiated on the basis that public libraries should serve its role as a supplier of intellectual informations to all users and the National Library of Korea should play a role as chief supporter of collection preservation in individual libraries. Situated in such context, this study attempted to investigate several aspects of the present conditions of Korea public libraries, including the current technical ability and technicians available for materials preservation and restoration, current policies for preservation and provisions for disasters, and opinions on establishment of national library preservation and restoration center. Data was drawn from a survey with 208 public libraries in the country. As a conclusion, this study discusses such as 1) securing collection management policies for material preservations and restorations at the level of individual libraries 2) building tripartite system for efficient and methodical material preservations and restorations including public libraries, regional central libraries and the National Library of Korea.

• Journal of Conservation Science
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• v.27 no.3
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• pp.261-268
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• 2011

Traditional paste materials and additives for investigation were selected through careful literature survey. We used the immersed glutinous rice flour, soybean as a traditional paste materials and a yellow wax, alum, and lime as additives. The effect of additives on viscosity, pH, adhesive strength, flexibility, antibacterial activities, mildew resistance, and conservation properties were examined. It was found that there was a relationship between the properties(viscosity, pH, and adhesive strength) and the characteristics of additives. Alum increased flexibilty but decreased viscosity, adhesive strength, and pH. Yellow wax deceased flexibilty, viscosity, adhesive strength, and pH. Lime increased flexibilty, viscosity, adhesive strength, and pH. The conservation properties increased by adding all additive. The present work was therefore carried out with the objective of offering the base datum for keeping traditional papers and textiles using traditional paste.

• Journal of Chest Surgery
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• v.30 no.6
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• pp.553-558
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• 1997

Arterial allografts have known advantages over prosthetic vascular conduit for treatment of heart valvular disease, congenital heart disease and aortic disease. Cell viability may play a role in determining the longterm outcome of allografts. Endothelial cell is one important part in determining the allograft viability. To evaluate the viability of endothelial cells using current allograft preservation technique, porcine heart valve leaflets and arterial wall were subjected to collagenase digestion. Single endothelial cell suspension was labeled with GSA-PITC(Griffonia simplicifolia agglutininfluorescein isothiocyan te), a vascular, endothelial cell specific marker. The cell suspension was washed and incubated with Pl(Propidium iodide), which does not bind with viable cells, Endothelial cell viability was evaluated by calculating the percentage of GSA-FITC(+) and Pl(-) group using flowcytometric analysis. Allografts were treated with $4^{\circ}C$ antibiotic solo!ion for 24 hours for sterilization. After this, half of allografts were stored in $4^{\circ}C$ RPMI 1640 with HEPES buffer culture medium with 10% fetal bovine serum for 1 to 14 days(Group I). Another half of allografts were cryopreserved with a currently used technique (Group II). During the procurement and sterilization of arterial allografts, 22.8% and 24.4% of endothelial cell viability declined, respectively. In Group I, 11.9% of endothelial cell viability declined further steadily during 14 days of storage. In Group II, 13.7% of endothelial cell viability declined. These results show that largest loss of endothelial cell viability occurs during the nitial process. After 14 days of arterial allograft storage under $4^{\circ}C$ nutrient medium or cryopreservation, about 40% of endothelial cell viability is maintained. There were no differences between the endothelial cell viability from aortic valve leaflet, pulmonic valve leaflets, aortic wall and pulmonic wall.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.107-108
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• 2003

This study was conducted to investigate the effects of dilution rate and stored temperature of semen at 5, 25 and 35$^{\circ}C$ on fertility in liquid rooster semen. At 5$^{\circ}C$ cold temperature, no significant difference were found in sperm mobilities on dilution rate(1:1, 1:3, 1.6) among treatments. Sperm mobility for the conservation of 3 hours at 25∼35$^{\circ}C$ were significantly higher for 1:3 and 1:6 dilution rate(semen：diluent) groups than for 1:1 dilution rate group(P<0.05). In Fertility results after artificial insemination with the conservation of 3 hours at 5∼25$^{\circ}C$ temperature, no significant difference were found in fertility on dilution rate among treatments. Fertilities after artificial insemination with the conservation of 3 hours at 35$^{\circ}C$ were significantly higher for 1.3 and 1:6 dilution rate(semen：diluent) groups than for 1：1 dilution rate group(P<0.05).

• Journal of Conservation Science
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• v.34 no.3
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• pp.227-233
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• 2018

Philosophical discussion and ethical approach have been crucial aspects in the rational decision-making of heritage conservation. This study analyses why and how they play an important role in determining the quality of conservation practice. It highlights the key concepts of conservation philosophy and ethics, as well as their relationship, in order to establish a constructive platform through which researchers may understand the role and importance of conservation philosophy and ethics. In conclusion, this study provides a set of recommendation for the government, academic experts, and conservators which will allow them to become actively involved in improving the quality of philosophical and ethical knowledge in the field of conservation.

• Journal of Korean Library and Information Science Society
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• v.35 no.3
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• pp.175-204
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• 2004

Digital information resource requires detailed metadata to ensure its long-term preservation and accessibility for future generations. This paper illuminated the characteristics of digital information resources, the concept of digital preservation and the necessity of metadata for long-term preservation of digital information resources. And this paper examined the 16 elements of RLG metadata for preserving digital information and the Reference Model for an Open Archival Information System(OATS) to support the interoperability of digital archiving. And then this study investigated concretely element sets of the preservation metadata for digital information resources in the Cedars and OCLC/RLG embodied within the OAIS framework. As result of the study, the paper suggested basic plan required to provide preservation metadata elements to preserve digital information resources in Korea.

• Proceedings of the KSFDT Conference
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• 2002.04a
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• pp.33-37
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• 2002

• Journal of Dairy Science and Biotechnology
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• v.7 no.1
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• pp.20-30
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• 1989

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.2
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• pp.243-249
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• 2001

This study was investigated to determine the antimicrobial effect of the ethanol extract of Quercus mongolica leaf on microbial growth. The ethanol extract at the concentration of $250\;\mu\textrm{g}/mL\;and\;500\;\mu\textrm{g}/mL$ inhibited the growth of gram positive and gram negative food-borne disease bacteria for 40 hours in tryptic soy broth, respectively. Antimicrobial activity of the ethanol extract from Quercus mongolica leaf was not affected by pH and heat treatment. The comparision between ethanol extract and commercially available preservatives on antimicrobial activity in food system was conducted. When the 0.1% ethanol extract of Quercus mongolica leaf was added to pine needle drink and carrot juice, antimicrobial activity was similar to those of containing 0.05% benzoic acid and 0.5% grapefruit seed extract. Also addition of 2~3% ethanol extract to the soybean paste inhibited the microbial growth up to 7 week, comparable to the inhibition of 2% ethanol. Thus, this results indicate that the ethanol extract of Quercus mongolica leaf may be useful as natural antimicrobial agents.