• Journal of Veterinary Clinics
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• v.30 no.4
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• pp.320-323
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• 2013

Spinal cord infarction is becoming recognized as an important cause of acute myelopathy in cats. Although the definitive diagnosis is confirmed through postmortem histopathologic examination, MR imaging features provide valuable informations for the diagnosis of spinal cord infarction. The aim of this report is to describe MR findings of acute spinal cord infarction in two cats and to evaluate usefulness of low field MRI (0.3Tesla) as a potential diagnostic tool of acute spinal cord infarction. A cat (unknown age, neutered male mixed breed cat) was referred one day after the acute onset of non-ambulatory spastic tetraparesis and the other cat (a 9-year-old, neutered female domestic short hair cat) was presented due to the acute onset of non-ambulatory paraparesis and one day later paraplegia. The lesions of the MR images were shown on the spinal cord parenchyma over C2 to C6 in case 1 and L2 to L5 in case 2. The MR images in these two cases were characterized by focal intramedullary lesions, mainly involving grey matter which were hyperintense T2 weighted and FLAIR images and hyperintense on DWI and hypointense on ADC map. The MR findings in both cases were highly suggestive of acute spinal cord infarctions, based upon previous reported small animal cases and human cases. In conclusion, based on MR features, together with the history and clinical examination findings, MRI modality can be used as an antemortem tool for the diagnosis of acute spinal cord infarction in cats.

• Journal of Preventive Medicine and Public Health
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• v.36 no.2
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• pp.117-124
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• 2003

Objectives : This study was performed to examine the relationship between maternal exposure to environmental tobacco smoke (ETS) and pregnancy outcomes (low birth weight or preform baby) in a prospective cohort study. Methods : We made a pregnant women's cohort, and followed the pregnancy outcomes, between May 1st 2001 and August 31st 2002. We surveyed 2,250 women who visited our hospital during their 35th gestational week, with a self-administered questionnaire. The final total of mother-infant pairs analyzed in this study was 1,712. We used a multiple logistic regression analysis to analyze the effect of maternal ETS on the incidence of preform or low birth weight, and a linear regression analysis for the birth weight and gestational age. Results : Higher exposure to ETS (>=1 hours/day) during pregnancy was more negatively associated with the gestational age and birth weight, than no exposure to ETS (no or less than 1 hour). Maternal exposure to ETS was associated with preform baby(adjusted odds ratio (AOR) 1.7; 95% confidence interval (CI) 0.9, 3.3) and low birth weight (AOR 2.3; 95% CI 0.9, 5.5). In addition, we found that maternal ETS may reduce the birth weight by 70g after adjusting for potential confounding factors. Conclusions : This study suggests that maternal exposure to ETS during pregnancy may increase the frequency of low birth weights and preform births.

• Journal of Oriental Neuropsychiatry
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• v.24 no.4
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• pp.419-434
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• 2013

Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric diseases such as dementia, for the mildly stroke rats, with 60 minutes of reperfusion transient middle cerebral artery occlusion (tMCAO). Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor behaviors were also observed with water maze tests. Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were observed with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate obvious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are mediated by neuro-protective effects through known antioxidant effects of components.

• Korean Journal of Veterinary Research
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• v.45 no.4
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• pp.469-475
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• 2005

Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=0.0005404D^2+0.04237D+0.00833$ [y = number of MN/cytokinesis-blocked (CB) cells and D = irradiation dose in Gy]. MN rates per 1,000 CB lymphocytes of pig from the HBRA (Cheongwon-gun, Boeun-gun) and the control area were $6.70{\pm}2.36$, $9.00{\pm}3.50$ and $11.00{\pm}2.98$, respectively. The MN frequencies of CB lymphocytes from pigs bred in three areas means that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the pigs were bred in the HBRA or the control area.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.243-248
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• 2016

RNase E (Rne) is an essential enzyme involved in the processing and degradation of a large portion of RNAs in Escherichia coli. The enzymatic activity of RNase E is controlled by regulators of ribonuclease activity, namely, RraA and RraB. Gram-positive bacterium Streptomyces coelicolor also contains homologs of Rne and RraA, designated as RNase ES (Rns), RraAS1, and RraAS2. In the present study, we investigated the effect of S. coelicolor RraAS1 on the ribonucleolytic activity of RNase E in E. coli. Coexpression of RraAS1 with Rne resulted in the decreased levels of rpsO, ftsZ, and rnhB mRNAs, which are RNase E substrates, and augmented the toxic effect of Rne overexpression on cell growth. These in vivo effects appeared to be induced by the binding of RraAS1 to Rne, as indicated by the results of co-immunoprecipitation analysis. These results suggested that RraAS1 induces ribonucleolytic activity of RNase E in E. coli.

• Journal of Conservation Science
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• v.34 no.5
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• pp.387-396
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• 2018

Exposure to the natural environment can cause damage to domestic wooden cultural assets, such as temples. Deterioration is accelerated by biological damage and various environmental factors. In this study, meteorological factors were monitored by equipment installed at Beopjusa temple of Boeun province and Seonamsa temple of Suncheon province. A statistical model was applied to these data to predict the meteorological factors and to compare the predictive performance of each meteorological factor. The resulting correlation coefficient between air and dew point temperatures was highest, at 0.95, while the correlation coefficient for relative humidity had a moderate value(0.65) at both the Beopjusa and Seonamsa temples. Thus, a general linear model was found to be suitable for predicting air and dew point temperatures. An analysis of correlation between meteorological factors showed that there was strong positive correlation between air temperature and dew point temperature, and between solar radiation and evaporation at both sites. There was a weak positive correlation between air temperature and evaporation at Beopjusa temple. Wind speed was negatively correlated with both air temperature and relative humidity at Seonamsa temple. The wind speed at this location is higher than average in winter and lower than average in summer, and it was hypothesized that the low wind speed plays a role in reducing water evaporation in summer, when both air temperature and relative humidity are high. As a result, damage to the wooden buildings of Seonamsa temple is accelerated.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.5
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• pp.564-573
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• 2006

This study was performed to examine the effect of particulate matter less than 10 ${\mu}m$ in diameter($PM_{10}$) on respiratory-related admission in Seoul, 1999. Daily counts of respiratory-related admission were analyzed by generalized additive model with adjustment for effects of air temperature, humidity, and day of the week as confounders in a nonparametric approach. The results follow associations between $PM_{10}$ and asthma, acute upper respiratory disease, acute lower respiratory disease, pneumonia, and chronic respiratory disease. The relative risks were 1.30(95% CI=1.14$\sim$1.50) for pneumonia, 1.18(95% CI=1.01$\sim$1.37) for acute lower respiratory disease in less than 15 years, respectively. The relative risks were 1.85(95% CI=1.22$\sim$2.81) for acute lower respiratory disease, 1.28(95% CI=1.04$\sim$1.57) for asthma, 1.25(95% CI=1.01$\sim$1.54) for pneumonia and 1.19(95% CI=1.01$\sim$1.41) for acute upper respiratory disease in 15 to 64 years, respectively The relative risks were 1.54(95% CI=1.15$\sim$2.08) for asthma, 1.38(95% CI=1.06$\sim$l.80) for chronic respiratory disease in more than 65 years, respectively. The study showed that $PM_{10}$ was considerably affects daily counts of respiratory-related admission in Seoul, 1999 Statistically significant associations were mostly found in the adult group like If to 64 years. The highly relative risks come out in the elderly.

• Journal of Life Science
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• v.28 no.5
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• pp.597-604
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• 2018

Although the core mechanisms of Attention Deficit/Hyperactivity Disorder (ADHD) are unknown, several ADHD-associated proteins have been studied. G-protein - coupled receptor kinase interacting protein-1 (GIT1) is a multifunctional adapter protein that affects neuron growth and dendrite formation. GIT1-deficient mice have shown ADHD-like behavior and also recovered through amphetamine treatment. In this study, gliotransmitters were investigated in both intracellular and extracellular space from GIT1-deficient mice. To measure the amount of gliotransmitters, primary astrocyte cultures were taken from the cerebral and cerebellar cortices of wild (WT), hetero (HE), and knock-out (KO) mice. Major gliotransmitters were analyzed using high-performance liquid chromatography. It was observed that the amount of excitatory and inhibitory gliotransmitters were dependent on genotype and showed a change in excitation/inhibition ratios. Interestingly, the major excitatory gliotransmitter, glutamate, existed at the lowest level in WT mice, but the amount of inhibitory gliotransmitters, gamma-aminobutyric acid (GABA) and glycine, varied depending on brain region. Remarkably, an increased amount of GABA was measured at the intracellular cerebrum in WT mice compared with KO mice. It was presumed that KO mice would secrete more inhibitory gliotransmitters to compensate for GIT1 depletion or else acquire a defect to reuptake-secreted GABA. This may be a possible mechanism for ADHD pathology.

• Journal of Veterinary Clinics
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• v.31 no.5
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• pp.449-453
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• 2014

A 2-year-old, intact male, Persian exotic cat, weighing 2 kg with a history of a palpated abdominal mass was admitted to Veterinary Medical Teaching Hospital, Seoul National University. On routine complete blood count (CBC) and serum biochemical analyses, there were anemia and 0.45 of albumin to globulin ratio. A feline infectious peritonitis (FIP) virus antibody test kit was negative. Radiography, ultrasonography and computed tomography (CT) were performed. A markedly enlarged abdominal mass was found. On cytologic examination of this mass, it was diagnosed as alimentary lymphoma or pyogranulomatous inflammation. Chemotherapeutic treatment for intestinal lymphoma was provided for several weeks, but the mass size was increased and clinical signs were not improved. The cat died six days after discontinuing chemotherapy. On postmortem examination, the definitive diagnosis was FIP. This case describes a cat with FIP in which an abdominal tumor had been suspected clinically.

• Journal of Conservation Science
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• v.32 no.4
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• pp.535-548
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• 2016

Hanging paintings are used for special ceremonies in Buddhist temple. They are stored in special storage box called "Gwaebulgwae" which means storage box for a hanging painting. Studies regarding pigments, background fibers and textiles of hanging paintings are carried out with many data in the field of conservation science. However, there are only few studies for the axes of hanging paintings and storage boxes. In this study, species identification for wooden axes of hanging paintings and storage boxes from the Joseon dynasty are carried out. Hanging painting of Beopjusa temple, Magoksa temple, Sudeoksa temple, Gaeamsa temple, Sudosa temple, Bukjangsa temple, Dorimsa temple, Tongdosa temple and Daeryeonsa temple are selected. Sampling is carried out from the axes of hanging paintings and storage boxes. 81 samples are collected from hanging paintings and storage boxes. As a result, 51 hard pines of Pinus spp., 8 Tilia spp., 6 Juniperus spp., 5 soft pines of Pinus spp., 4 Tsuga spp., 3 Pseudotsuga spp., 2 Berchemia spp., 1 Pyrus spp., 1 Bambusoideae are identified. Tsuga spp. and Pseudotsuga spp. might be used in conservation and restoration works in modern times because those species do not naturally grow in Korea. Most hanging paintings and storage boxes are made of hard pine. Also, some of are made of Tilia spp., because it represents the symbol of Buddha. Some unique kinds of trees used for wooden sculpture are identified, such as Juniperus spp. and Berchemia spp. Authentic conservation and restoration works could be carried out with this data-base.