• Journal of Korean Ophthalmic Optics Society
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• v.9 no.2
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• pp.381-389
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• 2004

To investigate changes of multi-purpose solutions for soft contact lens(MPS) depending on using period or keeping temperature, we evaluate four brands of MPS. No significant difference was seen in protein deposit removing efficacy after samples had used for 24 weeks and kept at $4^{\circ}C$, $20^{\circ}C$ or $30^{\circ}C$. The pH values of the samples of 4 brands measured weekly over the 24 week testing period. The initial average pH value of samples were 7.0, 7.5, 7.6 or 8.2. One brand of MPS was in the range of the threshold for ocular awareness, which is outside the zone of 6.6 ~ 7.8. During the testing period, the pH value were decreased in using period-dependent manner. At the 24th week, the average pH values of samples turned to 6.6, 7.2, 7.2 or 7.7. However, the difference of keeping temperature was not associated with decreased levels of pH values. After 24 weeks, one of total 36 samples was contaminated by bacteria. Furthermore, the change of components was shown after 24 weeks in the analysis using thin layer chromatography and the analysis of UV absorption pattern. The results of our study provides that the keeping temperature of MPS is not the important factor of changes of MPS, but the using period of MPS can cause contact lens wearers discomfort.

• Membrane Journal
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• v.22 no.5
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• pp.301-308
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• 2012

Phosphazene diagnostic membranes were prepared to measure blood glucose level of diabetics. Final absorbances at 680nm through activated phosphazene membranes were measured at various temperature and humidity by varing concentration of glucose in blood. Measuring temperature and humidity did not seriously affected the end-point results of varing absorbance values as time (K/S). The effects of storage temperatures on the measurements of glucose concentration were studied after activated phosphazene diagnostic membranes were stored at various temperatures for 3 days, 1 week, 3 weeks, and 5 weeks. The stabilities of phosphazene dianostic membranes were confirmed even at RH 80%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.8 no.1
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• pp.15-20
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• 1979

The nitrite and nitrate levels of carrot juice at various temperature and periods were studied. The nitrite level of carrot juice at high temperature increased rapidly as the bacterial level increased. When carrot juice was held at $30^{\circ}C$, nitrite concentration began to decline after 14 hours, although there was no decrease in bacterial population, The nitrate level of carrot juice at high temperature decreased rapidly. The bacteria in carrot juice were supposed to reduce nitrates to nitrites, No increase in nitrite and no decrease in nitrate occured when bacterial growth was prevented by holding the juice at $5^{\circ}C$ or by adding potassium dehydroacetate.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.101-101
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• 2001

• Korean Journal of Clinical Laboratory Science
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• v.53 no.4
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• pp.326-332
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• 2021

Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

• Korean Journal of Food Science and Technology
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• v.42 no.3
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• pp.373-376
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• 2010

The objective of this study was to present the proper probability distribution model based on the data obtained from surveys on domestic refrigerator food storage temperatures in home. Domestic refrigerator temperatures were determined as risk factors in foodborne disease outbreaks for microbial risk assessment (MRA). The temperature was measured by directly visiting 139 homes using a data logger from May to September of 2009. The overall mean temperature for all the refrigerators in the survey was $3.53{\pm}2.96^{\circ}C$, with 23.6% of the refrigerators measuring above $5^{\circ}C$. Probability distributions were also created using @RISK program based on the measured temperature data. Statistical ranking was determined by the goodness of fit (GOF, i.e., the Kolmogorov-Smirnov (KS) or Anderson-Darling (AD) test) to determine the proper probability distribution model. This result showed that the LogLogistic (-10.407, 13.616, 8.6107) distribution was found to be the most appropriate for the MRA model. The results of this study might be directly used as input variables in exposure evaluation for conducting MRA.

• Membrane Journal
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• v.17 no.1
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• pp.31-36
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• 2007

Polyurethane diagnostic membranes were prepared to measure blood glucose level of diabetics. Final absorbances at 680 nm through activated polyurethane membranes were measured at various concentration of glucose in plasma or blood. The effects of storage temperatures on the measurements of glucose concentration were studied after storage of 3 days, 1 week, 3 weeks, and 5 weeks at various temperatures. The stabilities of polyurethane diagnostic membranes were examined at RH 80%.

• Applied Microscopy
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• v.42 no.1
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• pp.43-48
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• 2012

This study shows that ceresin wax, candellila wax and microcrystalline wax mixed together with liquid paraffin oil to produce lipsticks (LS-1, LS-2) and capric/caprylic triglyceride oil added to produce lipsticks (LS-3, LS-4). After each type of lipsticks were molded, LS-1 and LS-3 was put into a cooling chamber ($5^{\circ}C$). LS-2 and LS-4 was put into a cooling chamber ($5^{\circ}C$) for 18 hours and kept in an incubator ($45^{\circ}C$) for 5 hours and put again into a cool chamber ($5^{\circ}C$). After that, the wax's three dimensional network structure was observed under scanning electron microscopy. Regardless of the kind of oil, the LS-1 and LS-3 wax structure had more distinct shape than the lipstick wax structure of LS-2 and LS-4. Also, regardless of the kind of wax, the three dimensional network structure was modified as the storage temperature increased. As a result, the lipstick's molding temperature increased, the wax's structure size also increased and the shape irregularly modified. This modification causes sweating phenomenon which affected lipstick's surface rheological property.

• The Korean Journal of Nuclear Medicine Technology
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• v.25 no.1
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• pp.29-33
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• 2021

Purpose Plasma renin activity (PRA) test is important for the diagnosis of primary aldosteronism. PRA is an easily deformed substance in vitro and affected by temperature changes. Laboratory of ASAN medical center has consistently found that there was a difference between the initial and re-experimental results. We compared and analyzed the differences in PRA test results according to the sample storage status. Materials and Methods The measurement of PRA was performed by using the radioimmunoassay. From August to September 2020, 43 PRA re-test samples were tested with different sample storage condition. The first group was re-examined by freezing the plasma-separated samples at -18℃, and the second group was re-examined with refrigerated EDTA sample. Also, additional tests were conducted on 13 PRA samples to verify the effect on thawing temperature differences in plasma-separated samples. The same samples were divided into two parts and stored frozen at -18℃, respectively, and thawing samples in room temperature and those in refrigerator were were conducted. Each result was compared and analyzed based on the initial experimental results. Results The results of re-examination after frozen storing plasma separation samples showed a lower correlation than the results of re-examination with EDTA plasma samples in refrigerator. When calculating the percentage based on the initial test results, the average percentage of each was 404.9% and 133.8%. The correlation coefficient was also R=0.8501 and R=0.9966, respectively, showing a higher correlation between plasma in the refrigerated sample EDTA tube. In comparison experiments with differences in thawing temperature, average percentage of the results of initial test and room temperature thawing was 94.3% and the average percentage of the results of refrigerated thawing was 88.0%. After again freezing the sample, the average percentage of the second room temperature thawing result is 107.5%, and the second refrigerated thawing group is 112.7%. Both groups showed an increase from first thawing. Conclusion A comparative analysis of retesting according to differences in sample storage methods in PRA tests showed a higher correlation between the results of retesting of the refrigerated EDTA plasma. And repeated freezing and melting of plasma separation samples, regardless of temperature during defrosting, has been shown to affect results. Therefore, retest of PRA should re-collect plasma from original EDTA plasma to increase reproducibility.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.2
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• pp.277-281
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• 1997

The cell density changes of Vibrio mimicus K-1 in sea water and arkshell feeding it were examined at various temperature. The strain was suspended in sterilized sea water and storaged at experimental temperature $(5,\;10,\;15,\;20,\;and\;28^{\circ}C)$). At intervals of up to 10 days, aliquots of each suspension were plated onto BHI agar. At 5 and $10^{\circ}C$, the plate counts of V. mimicus K-1 showed a rapid decline, which 3s known to be a reault of this bacterium's entering into the viable but non culturable state. At 20 and $28^{\circ}C$, however, V. mimicus K-1 are stable over the 10 days experimental periods. V. mimicus K-1 was fed to arkshell, which was subsequently stored at temperatures ranging from 5 to $20^{\circ}C$ for 10 days. The samples of arkshell were homogenized and plated at intervals to determine the cell density of V. mimicus K-1 and total aerobic population of bacteria present. At 5 and $10^{\circ}C$, the numbers of V. mimicus K-1 in sea water rapid decreased over the 10 days experimental periods. However, little change of V. mimicus K-1 density was observed in shellstock arkshell at 5 and $10^{\circ}C$. While, V. mimicus K-1 density was decreased more rapidly to level below limit of dectection in shucked arkshell at same temperature. Incubation at the higher temperature $(20^{\circ}C)$ resulted in large increase in total aerobic bacterial number of shellstock arkshell. These results suggest that even with proper storage, indigenous levels of V. mimicus may remain sufficiently high in shellstock arkshell to produce infection in compromise hosts.