대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)

  • 제53권4호
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  • Pages.326-332
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  • 2021
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  • 1738-3544(pISSN)
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  • 2288-1662(eISSN)
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)
권호 표지
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면봉시료에서 세균의 보존을 위한 최적 보관 온도와 채취 시약의 비교

Comparison of Optimal Storage Temperature and Collection Reagents for Living Bacterial Cells in Swab Samples

  • 이영주 (을지대학교대학원 임상병리학과) ;
  • 유희상 (을지대학교대학원 BK21 프로그램 시니어헬스케어학과) ;
  • 이송희 (을지대학교대학원 임상병리학과) ;
  • 이소립 (을지대학교대학원 BK21 프로그램 시니어헬스케어학과) ;
  • 이한 (을지대학교 임상병리학과) ;
  • 성호중 (을지대학교대학원 임상병리학과) ;
  • 강희규 (을지대학교대학원 임상병리학과) ;
  • 현성희 (을지대학교대학원 임상병리학과)
  • Lee, Yeong Ju (Department of Biomedical Laboratory Science, Graduate School, Eulji University) ;
  • You, Hee Sang (Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University) ;
  • Lee, Song Hee (Department of Biomedical Laboratory Science, Graduate School, Eulji University) ;
  • Lee, So Lip (Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University) ;
  • Lee, Han (Department of Biomedical Laboratory Science, Eulji University) ;
  • Sung, Ho Joong (Department of Biomedical Laboratory Science, Graduate School, Eulji University) ;
  • Kang, Hee Gyoo (Department of Biomedical Laboratory Science, Graduate School, Eulji University) ;
  • Hyun, Sung Hee (Department of Biomedical Laboratory Science, Graduate School, Eulji University)
  • 투고 : 2021.10.22
  • 심사 : 2021.12.02
  • 발행 : 2021.12.31
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초록

면봉을 사용한 샘플링 방법은 의학, 생태학, 생명공학, 법의학 및 오염 정도 모니터링 시스템과 같은 다양한 연구 분야에서 유용하다. 샘플링에서 채취 시약은 중요한 요소 중 하나이다. 시료를 장기간 보관해야 하는 경우에는 시료 채취 키트와 시료 보관 온도가 매우 중요하다. 이 연구의 목적은 별도의 배지 없이, 세 가지 채취 시약과 세 가지 보관 온도가 살아있는 세균의 생균 수에 미치는 영향을 확인하는 것이다. 대표적인 환경 세균으로 E. coli와 S. aureus를 선정하였다. 증류수(DW), 멸균된 인산염 완충액(PBS), Tris-EDTA (TE) 버퍼를 채취 시약으로 사용하여 샘플링한 후, 22, 4, -70℃에서 보관을 진행했다. 각 채취시약 및 보관 온도가 시료에 미치는 영향은 RLU와 CFU로 결과로 비교하였다. -70℃ 보관 온도와 TE 버퍼를 사용할 때 CFU와 RLU 값은 다른 조건에서보다 일정하게 유지되는 경향이 보였다. 따라서 이 연구는 시료가 채취 직후 -70℃에서 보관되어야 하며, 채취 시약으로 TE 버퍼와 함께 사용하는 것이 좋다는 것을 시사한다.

Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

키워드

과제정보

This research was supported and funded by the Korean National Police Agency. [Project Name: Development of bloodstain analysis system for scene reconstruction/Project Number: POLICE_I_00001_01_101]. This paper was supported by Eulji University in 2021

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