• Journal of Life Science
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• v.22 no.9
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• pp.1254-1260
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• 2012

The moringa (Moringa oleifera Lam.) plant is used both as food and an anti-allergic agent. In this study, we investigated skin protection effects of methanol extracts from the root, seed, fruit, and leaves of moringa in HaCaT keratinocyte cells. To investigate the pharmacological potential of various moringa extracts on TNF-${\alpha}$-induced collagen degradation in HaCaT cells, we measured the activity of matrix metallopeptidase-9,2 (MMP-9,2) by zymography analysis. Our results showed that all the moringa extracts inhibit the TNF-${\alpha}$-induced enzyme activity of MMP-9. In particular, moringa root extracts significantly suppressed MMP-9 and MMP-2 in a dose-dependent manner. Next, to investigate the anti-inflammation effect of the moringa extracts, we examined cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and interleukin-6 (IL-6) expression of the extracts. The results showed that both the root extracts and the seed extracts decreased the TNF-${\alpha}$-induced expression of COX-2. In addition, the root and leaf extracts reduced the expression of IL-6. However, none of the moringa extracts affected the expression of iNOS. The results suggest that moringa root extracts down-regulate MMP-9, COX-2, and IL-6 and that the root extracts offer superior skin protection effects compared with other extracts of moringa in HaCaT cells.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1319-1325
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• 2000

Antimicrobial effects of the specific IgY separated from eggs which were laid by hens vaccinated with Streptococcus mutans were investigated. The comparison tests of vaccination, addition levels of crude specific IgY, and innoculation concentration were applied by microscopic observation and turbidity test. Ten% addition of crude specific IgY obtained from vaccinated hens showed agglomerative clusters of S. mutans cells in supernatants and sediments, while crude IgY produced by non-vaccinated hens showed no cluster. IgY addition above 5% showed agglutinating clusters of most S. mutans cells and there was definite difference between IgY addition below 2.5% and above 5%. Concentration tests of crude IgY revealed that antimicrobial effects were differentiated by addition level and addition over 10% produced satisfactory results with turbidity test. The cluster size was dependent upon concentration of crude IgY addition. $10^5\;cfu/mL$ inoculation showed agglutinated cells and extent of agglomeration was proportional to cell numbers. Study of inoculation levels showed that 10% addition of crude IgY decreased turbidity effectively regardless of number of S. mutans cells. Plaque formation decreased to 75% with 15% addition of specific IgY concentration. These results implied that IgY separated from eggs laid by S. mutans-vaccinated hens might prevent dental caries caused by S. mutans.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.484-491
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• 2023

Maize is one of the major crops consumed in worldwide, which nutrients accounts for a large amount of starch, but also functional components, and phenolic acid is known to have a high content. Maize is divided into waxy maize, sweet maize, and normal maize with its shape and use, therefore there is also a difference in nutritional composition. This study was conducted to analyze the content of functional components according to the type of maize and to produce natural variation data in consideration of environmental factors. 3 shapes of maize (waxy maize, sweet maize, and normal maize) samples cultivated in 3 regions (Suwon, Daegu, and Hongcheon) were analyzed using HPLC and GC-TOF-MS. Comparing with type through ANOVA, multivariate statistical analysis, Pearson correlation analysis, 28 components, including carotenoids and tocopherols, showed significant differences among a total of 32 components (p <0.05), 15 of them showed very significant differences (p <0.001). When comparing with regions, 15 components showed significant differences and only vanillate, syringate, C23-ol of them showed most significant differences (p <0.001). As a result of principal component analysis, cluster classification was distinguished by shape than by region, with α-carotene, cholesterol for waxy maize, vanillate and stigmasterol for sweet maize, lutein and β-carotene for normal maize had a great effect on cluster formation. It suggests that the content of functional components is more affected by genetic factors than environmental factors.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.46-52
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• 2014

This study was conducted to compare colony forming unit (CFU) of microorganisms in closed and open soilless culture systems for estimating the possibility for potential disease occurrence. Samples were collected at four different positions in four commercial greenhouses with closed or open soilless culture system using rock wool or coir as substrate, respectively. The distance between sampling positions was 3 cm starting from the substrate and the surface area of each position was $25cm^2$. The CFU of fungi was significantly higher in the open system, while that of bacteria was not significantly different but showed relatively lower in the closed system. Samples collected at the plastic surface of the substrates where little environmental effects occurred from drainage showed lower CFU than any other positions. The principal component analysis showed that samples collected on the drainage pathway highly affected the changes in microbial population in the greenhouse. These results indicated that greenhouses with closed soilless culture are expected to have more advantageous conditions for restraining the microbial growth, resulting in the lower potential of disease occurence in greenhouse ecosystem.

• Current Research on Agriculture and Life Sciences
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• v.24
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• pp.23-27
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• 2006

Seedling characteristics of rice on nursery covered with different weight of polypropylene spunbonded fabric(PSF) were investigated to determine the optimum thickness of PSF for raising of healthy rice seedlings. Four different thickness of PSF 40, 60, 80 and $100g/m^2$ were used in the seedling nursery with a conventional polyethylene(PE) film. PSF and PE were taken off in 20 days after sowing(DAS) on April and in 10 DAS on June. Plant height of 20- and 30- day old seedlings in PSF covering nursery was slightly taller as the increase of PSF thickness from $40g/m^2$ to $100g/m^2$, but number of leaves and shoot dry-weight of the seedlings was reduced. Root solidity of seedlings grown in lighter PSF ($40{\sim}60g/m^2$) covering nursery was more favorable than that in relatively heavier PSF($80{\sim}100g/m^2$). The optimum thickness of PSF for the raising of healthy seedlings was $40{\sim}60g/m^2$ PSF at sowing on April 20 and April 30 for single cropping, and $40g/m^2$ PSF on June for double cropping based on the growth characteristics of seedlings investigated at seedling nursery covered with different thickness of PSF.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.117-129
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• 2020

The body color pattern in fish is a distinctive feature for species identification. The blass bloched rockfish Sebastes pachycephalus is a commercially important marine fish species, distributed in the central and southern parts of Korea and south Hokkaido of Japan. It has a morphological feature divided into four subspecies according to with or lacking distinct spots on the body surface, and to the location of markings on the body surface. However, the genetic basis of body color pattern of S. pachycephalus is still unknown. Thus we analyzed the transcriptome of S. pachycephalus skin samples using RNA-seq analysis to investigate functional genes related to body color patterns. The experimental skin samples were prepared by classified into 'Wild type' (lacking distinct spots and markings) and 'Color type' (with distinct spots and marking). Two skin sample transcriptomes were compared pairwise and the results revealed that were 164 differentially expressed unigenes in the skin samples of 'Wild type' and 'Color type'. Gene Ontology analysis of 164 differentially expressed unigenes showed that these genes were included in the functional group of molecular function (2 genes), biological process (46 genes), and cellular component (6 genes). There were several genes that body color type skin specific expression and the genes were CTL (Galactose-specific lectin nattectin), CUL1 (Cullin-1), CMAS (N-acylneuraminate cytidylyltransferase), NMRK2 (Nicotinamide riboside kinase 2), ALOXE3 (Hydroperoxide isomerase ALOXE3), SLC4A7 (sodium bicarbonate cotransporter 3). Our study is the first attempt to search for functional genes involved in the formation of body color patterns in S. pachycephalus. The differentially expressed unigenes obtained in this study can be used as candidate genes for functional gene study related to body coloration of fish.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.43 no.5 s.311
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• pp.89-100
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• 2006

The carotid intima-media thickness (IMT) is very important, because the severity of it is an independent predictor of transient cerebral ischemia, stroke, and coronary events such as myocardial infarction. The conventional image processing to measure the IMT has not been satisfactory, because the methods have relied on the manual section drawing and a regional segmentation by differential estimation. We propose a new image processing technology effective to extract features from the carotid artery image whose pixels have the directional vector properties with composed color distribution. The technique we presented here is not by differential variation but by verification of the layer properties of carotid artery image. Iterated vertical and horizontal analysis and segmentation of the IMT image show the vector characteristics. This new technique makes it possible to cluster the layers statistically, and to classify mathematical correlation between regions and resulting in correct measurements of thickness and its variation. The advantages and effectiveness of this approach are applicable to region process and character extraction of such a vector image.

• Journal of The Korean Association For Science Education
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• v.34 no.3
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• pp.273-283
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• 2014

The purpose of this study was to examine the potential of the use of the Classroom Response System (CRS), a kind of new ICT medium, in a quiz problem-solving oriented high school life science class. To find the usefulness of CRS as a teaching and learning strategy, the CRS group (n=34) sent prompt individual answers to the teachers' questions using the CRS terminal (Clicker), and the teacher then asked additional reasons of the individuals and gave personalized feedback. In the control group (n=35), the CRS was not used while the teacher asked overall questions and gave feedback in an undifferentiated way. As a result, the CRS increased students' interest and concentration during class, but there were no significant differences in study achievement between the two groups. However, there were significant differences between the medium-level groups when the two groups were divided into smaller ones based on their pre-scores. We suggest that, for effective use of the CRS for diagnostic and formative assessment, teachers should develop a teaching and learning strategy that can produce appropriate questions of various levels in advance, investigate the exact reasons for students' answers, and give customized feedback by individual as much as possible.

• Korean journal of food and cookery science
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• v.18 no.6
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• pp.716-722
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• 2002

Meat(beef, pork, chicken, duck) were cooked by three kinds of instruments (gas grill. electric grill, microwave oven) and extracted with 80% methanol. These methanol extracts were performed the Ames test, employing S. typhimurium tester strain TA100 (in vitro) and micronucleus test (in vivo). The methanol extract of cooked pork showed high mutagenicity in 5.0mg/plate without S9 mix and induced a higher mutagenicity with S9 mix than without S9 mix at 5 mg/plate. In all kinds of cookery methods, pork extracts showed high mutagenicity according to increase of cookery temperature (200$\^{C}$, 260$\^{C}$ and 320$\^{C}$). The methanol extract of cooked pork by electric grill (at 260$\^{C}$, for 5 min) showed high mutagenicity in all kinds of cookery instruments on the Ames test and micronucleus test. In all kinds of meat, the methanol extract of cooked pork showed a higher mutagenicity than the others and chicken showed a lower. The extract after pork soaked in ginger juice showed lower mutagenicity and micronucleus formation than the other vegetable juice.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.4
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• pp.421-428
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• 2012

In this study, the effects of surface oxide film formation on the fatigue behavior of 7075-T6 aluminum alloy were analyzed in terms of the corrosion time of the alloy. The aluminum material used is known to have high corrosion resistance due to the passivation phenomenon that prevents corrosion. Aluminum alloys have been widely used in various industrial applications such as aircraft component manufacturing because of their lighter weight and higher strength than other materials. Therefore, studies on the fatigue behavior of materials and passivation properties that prevent corrosion are required. The fatigue behavior in terms of the corrosion time was analyzed by using a four-pointing bending machine, and the surface corrosion level of the aluminum material in terms of the corrosion time was estimated by measuring the surface roughness. In addition, fractographic analysis was performed and the oxide films formed on the material surface were studied by scanning electron microscopy (SEM). The results indicated that corrosion actively progressed for four weeks during the initial corrosion phase, the fatigue life significantly decreased, and the surface roughness increased. However, after four weeks, the corrosion reaction tended to slow down due to the passivation phenomenon of the material. Therefore, on the basis of SEM analysis results, it was concluded that the growth of the surface oxide film was reduced after four weeks and then the oxide film on the material surface served as a protection layer and prevented further corrosion.