Browse > Article
http://dx.doi.org/10.5352/JLS.2012.22.9.1254

Suppression of TNF-α-induced Inflammation by Extract from Different Parts of Moringa in HaCaT Cells  

Lee, Hyo-Jin (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine)
Chang, Young-Chae (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine)
Publication Information
Journal of Life Science / v.22, no.9, 2012 , pp. 1254-1260 More about this Journal
Abstract
The moringa (Moringa oleifera Lam.) plant is used both as food and an anti-allergic agent. In this study, we investigated skin protection effects of methanol extracts from the root, seed, fruit, and leaves of moringa in HaCaT keratinocyte cells. To investigate the pharmacological potential of various moringa extracts on TNF-${\alpha}$-induced collagen degradation in HaCaT cells, we measured the activity of matrix metallopeptidase-9,2 (MMP-9,2) by zymography analysis. Our results showed that all the moringa extracts inhibit the TNF-${\alpha}$-induced enzyme activity of MMP-9. In particular, moringa root extracts significantly suppressed MMP-9 and MMP-2 in a dose-dependent manner. Next, to investigate the anti-inflammation effect of the moringa extracts, we examined cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and interleukin-6 (IL-6) expression of the extracts. The results showed that both the root extracts and the seed extracts decreased the TNF-${\alpha}$-induced expression of COX-2. In addition, the root and leaf extracts reduced the expression of IL-6. However, none of the moringa extracts affected the expression of iNOS. The results suggest that moringa root extracts down-regulate MMP-9, COX-2, and IL-6 and that the root extracts offer superior skin protection effects compared with other extracts of moringa in HaCaT cells.
Keywords
Inflammation; metallopeptidase-9,2 (MMP-9,2); cyclooxygenase-2 (COX-2); moringa (Moringa oleifera Lam.);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Aktan, F. 2004. iNOS-mediated nitric oxide production and its regulation. Life Sci. 75, 639-653.   DOI
2 Anggakusuma, Yanti and Hwang, J. K. 2010. Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells. J. Dermatol. Sci. 57, 114-122.   DOI
3 Anwar, F., Latif, S., Ashraf, M. and Gilani, A. H. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Phytother. Res. 21, 17-25.   DOI   ScienceOn
4 Atawodi, S. E., Atawodi, J. C., Idakwo, G. A., Pfundstein, B., Haubner, R., Wurtele, G., Bartsch, H. and Owen, R. W. 2010. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J. Med. Food 13, 710-716.   DOI   ScienceOn
5 Bachmeier, B. E. and Nerlich, A. G. 2002. Immunohistochemical pattern of cytokeratins and MMPs in human keratinocyte cell lines of different biological behaviour. Int. J. Oncol. 20, 495-499.
6 Beissert, S., Cavazzana, I., Mascia, F., Meroni, P., Pastore, S., Tessari, G. and Girolomoni, G. 2006. Mechanisms of immune- mediated skin diseases: an overview. Clin. Exp. Rheumatol. 24, S1-6.
7 Buckman, S. Y., Gresham, A., Hale, P., Hruza, G., Anast, J., Masferrer, J. and Pentland, A. P. 1998. COX-2 expression is induced by UVB exposure in human skin: implications for the development of skin cancer. Carcinogenesis 19, 723-729.   DOI   ScienceOn
8 Diker-Cohen, T., Koren, R., Liberman, U. A. and Ravid, A. 2003. Vitamin D protects keratinocytes from apoptosis induced by osmotic shock, oxidative stress, and tumor necrosis factor. Ann. N. Y. Acad. Sci. 1010, 350-353.   DOI
9 Eberhardt, W., Huwiler, A., Beck, K. F., Walpen, S. and Pfeilschifter, J. 2000. Amplification of IL-1 beta-induced matrix metalloproteinase-9 expression by superoxide in rat glomerular mesangial cells is mediated by increased activities of NF-kappa B and activating protein-1 and involves activation of the mitogen-activated protein kinase pathways. J. Immunol. 165, 5788-5797.   DOI
10 Faizi, S., Siddiqui, B. S., Saleem, R., Siddiqui, S., Aftab, K. and Gilani, A. H. 1994. Isolation and structure elucidation of new nitrile and mustard oil glycosides from Moringa oleifera and their effect on blood pressure. J. Nat. Prod. 57, 1256-1261.   DOI   ScienceOn
11 Han, Y. P., Tuan, T. L., Hughes, M., Wu, H. and Garner, W. L. 2001. Transforming growth factor-beta - and tumor necrosis factor-alpha -mediated induction and proteolytic activation of MMP-9 in human skin. J. Biol. Chem. 276, 22341-22350.   DOI   ScienceOn
12 Faizi, S., Siddiqui, B. S., Saleem, R., Siddiqui, S., Aftab, K. and Gilani, A. H. 1995. Fully acetylated carbamate and hypotensive thiocarbamate glycosides from Moringa oleifera. Phytochemistry 38, 957-963.   DOI   ScienceOn
13 Grewe, M., Trefzer, U., Ballhorn, A., Gyufko, K., Henninger, H. and Krutmann, J. 1993. Analysis of the mechanism of ultraviolet (UV) B radiation-induced prostaglandin E2 synthesis by human epidermoid carcinoma cells. J. Invest. Dermatol. 101, 528-531.   DOI
14 Guevara, A. P., Vargas, C., Sakurai, H., Fujiwara, Y., Hashimoto, K., Maoka, T., Kozuka, M., Ito, Y., Tokuda, H. and Nishino, H. 1999. An antitumor promoter from Moringa oleifera Lam. Mutat. Res. 440, 181-188.   DOI
15 Heck, D. E., Laskin, D. L., Gardner, C. R. and Laskin, J. D. 1992. Epidermal growth factor suppresses nitric oxide and hydrogen peroxide production by keratinocytes. Potential role for nitric oxide in the regulation of wound healing. J. Biol. Chem. 267, 21277-21280.
16 Hur, S., Lee, Y. S., Yoo, H., Yang, J. H. and Kim, T. Y. 2010. Homoisoflavanone inhibits UVB-induced skin inflammation through reduced cyclooxygenase-2 expression and NF-kappaB nuclear localization. J. Dermatol. Sci. 59, 163-169.   DOI
17 Ishida, H., Ray, R. and Ray, P. 2008. Sulfur mustard downregulates iNOS expression to inhibit wound healing in a human keratinocyte model. J. Dermatol. Sci. 49, 207-216.   DOI
18 Isoherranen, K., Punnonen, K., Jansen, C. and Uotila, P. 1999. Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes. Br. J. Dermatol. 140, 1017-1022.   DOI
19 Kahari, V. M. and Saarialho-Kere, U. 1997. Matrix metalloproteinases in skin. Exp. Dermatol. 6, 199-213.   DOI   ScienceOn
20 Johansson, N., Westermarck, J., Leppa, S., Hakkinen, L., Koivisto, L., Lopez-Otin, C., Peltonen, J., Heino, J. and Kahari, V. M. 1997. Collagenase 3 (matrix metalloproteinase 13) gene expression by HaCaT keratinocytes is enhanced by tumor necrosis factor alpha and transforming growth factor beta. Cell Growth Differ. 8, 243-250.
21 Kean, W. F. and Buchanan, W. W. 2005. The use of NSAIDs in rheumatic disorders 2005: a global perspective. Inflammopharmacology 13, 343-370.   DOI   ScienceOn
22 Kobayashi, T., Hattori, S., Nagai, Y., Tajima, S. and Nishikawa, T. 1998. Differential regulation of MMP-2 and MMP-9 gelatinases in cultured human keratinocytes. Dermatology 197, 1-5.   DOI
23 Kwon, D. J., Bae, Y. S., Ju, S. M., Goh, A. R., Choi, S. Y. and Park, J. 2011. Casuarinin suppresses TNF-alpha-induced ICAM-1 expression via blockade of NF-kappaB activation in HaCaT cells. Biochem. Biophys. Res. Commun.
24 Lawrence, T., Willoughby, D. A. and Gilroy, D. W. 2002. Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat. Rev. Immunol. 2, 787-795.   DOI
25 Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y., Furuta, S., Suda, I. and Sato, T. 2002. Polymeric procyanidins as radical- scavenging components in red-hulled rice. J. Agric. Food Chem. 50, 7524-7529.   DOI   ScienceOn
26 Rathi, B. S., Bodhankar, S. L. and Baheti, A. M. 2006. Evaluation of aqueous leaves extract of Moringa oleifera Linn for wound healing in albino rats. Indian J. Exp. Biol. 44, 898-901
27 Roig, R., Cascon, E., Arola, L., Blade, C. and Salvado, M. J. 2002. Procyanidins protect Fao cells against hydrogen peroxide-induced oxidative stress. Biochim. Biophys. Acta. 1572, 25-30.   DOI
28 Sirsjo, A., Karlsson, M., Gidlof, A., Rollman, O. and Torma, H. 1996. Increased expression of inducible nitric oxide synthase in psoriatic skin and cytokine-stimulated cultured keratinocytes. Br. J. Dermatol. 134, 643-648.   DOI   ScienceOn
29 Sakai, M., Shimizu, Y., Nagatsu, I. and Ueda, H. 1996. Immunohistochemical localization of NO synthases in normal human skin and psoriatic skin. Arch. Dermatol. Res. 288, 625-627.   DOI   ScienceOn
30 Schaffer, M. R., Tantry, U., van Wesep, R. A. and Barbul, A. 1997. Nitric oxide metabolism in wounds. J. Surg. Res. 71, 25-31.   DOI   ScienceOn
31 Staples, G. and Herbst, D. R. 2005. A tropical garden flora : plants cultivated in the Hawaiian Islands and other tropical places. Honolulu, Hawai'i: Bishop Museum Press. p xxiv, 908 p.
32 Ursini, F., Rapuzzi, I., Toniolo, R., Tubaro, F. and Bontempelli, G. 2001. Characterization of antioxidant effect of procyanidins. Methods Enzymol. 335, 338-350.   DOI
33 Vane, J. R. 1971. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat. New Biol. 231, 232-235.   DOI
34 Williams, T. J. 1983. Interactions between prostaglandins, leukotrienes and other mediators of inflammation. Br. Med. Bull. 39, 239-242.
35 Woessner, J. F. Jr. 2002. MMPs and TIMPs--an historical perspective. Mol. Biotechnol. 22, 33-49.   DOI
36 Yayeh, T., Oh, W. J., Park, S. C., Kim, T. H., Cho, J. Y., Park, H. J., Lee, I. K., Kim, S. K., Hong, S. B., Yun, B. S. and Rhee, M. H. 2011. Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells. J. Nat. Med. 66, 49-54.