• Applied Biological Chemistry
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• v.40 no.4
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• pp.277-282
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• 1997

Acetolactate synthase (ALS) was partially purified from Escherichia coli MF2000/pTATX containing Arabidopsis thaliana ALS gene. The partially purified ALS was examined for its sensitivity toward various modifying reagents such as iodoacetic acid, iodoacetamide, N-ethylmaleimide (NEM), 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), p-chloromercuribenzoic acid (PCMB), and phenylglyoxal. It was found that PCMB inhibited the enzyme activity most strongly followed by DTNB and NEM. Since iodoacetic acid did not compete with substrate pyruvate, it appeared that cysteine is not involved in the substrate binding site. On the other hand, the substrate protected the enzyme partly from inactivation by phenylglyoxal, which might indicate interaction of arginine residue with the substrate. The partially purified enzyme was inhibited by end products, valine and isoleucine, but not by leucine. However, the ALS modified with PCMB led to potentiate the feedback inhibition of all end products. Additionally, derivatives of pyrimidyl sulfur benzoate, a candidate for a new herbicide for ALS, were examined for their inhibitory effects.

• Korean Journal of Microbiology
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• v.31 no.4
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• pp.279-285
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• 1993

Alterations of the p53 gene arc among the most frequent genetic changes in human cancer and often result in increased levels of p53 protein within the malignant cells. Detection of accumulated p53 protein can be a useful prognostic tool in human cancer. In order to make polyclonal antibodies for immunohistochemical screening. human p53 gene was expressed in E. coli in the form of GST (glutathione S-transfi.:rase) fusion proteins. Two p53 gene fragments. which were N('()I small fragment encoding amino acid residues of 1-151-: and Ncol large fragment of 159-393. were subeloned into the unique BamHI site present within the pGEX-2T vector using BamHI linker and recombinant plasmids pGTNS and pGTNL were constructed. respectively. The p53 cDNA fragment (from pC53-$SN_3$,) encoding amino acid 38-145 (proline at residue 72) was amplified by polymerase chain reaction(PCR). The amplified DNA was digested with BamHI and Prull and inserted into the BamHI-Smal sites of pG EX-2T and recombinant plasmid pGTBP was constructed. After IPTG induction of these plasmids for 4 hours. fusion proteins were purified from E. coli extracts with glutathione Sepharose beads. The bound proteins were resolved by 10% SDS-polyacrylamide gel electrophoresis and the molecular weights were 54 kDa. 53 kDa and 40 kDa. respectively. Approximately one milligram of fusion proteins were purified from 1 -liter cultures.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.155-155
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• 2011

그래핀(graphene)은 모든 탄소 원자가 표면에 존재하는 이차원 결정이기 때문에 다른 고체 표면에 고착될 때 인장 및 압축 변형(tensile & compressive strain)과 전하 도핑(charge doping)에 취약하다고 알려져 있다. 본 연구에서는 산화실리콘(SiO2/Si) 기판 위에 기계적으로 박리된 그래핀에 혼재되어 나타나는 기계적 변형과 전하 도핑 현상을 분석할 수 있는 라만 분광법 기술을 개발하고자 하였다. 대부분의 시료에서 기계적 변형으로 인해 라만 G-band와 2D-band의 진동수(${\omega}$)가 특별한 상관관계(${\Delta}{\omega}2D/{\Delta}{\omega}G$ = 2.0 ${\pm}$ 0.2)를 가진다는 사실을 확인하였다. 전자 친화도가 큰 F4-TCNQ (tetrafluorotetracianoquinodimethane)를 증착하여 화학적으로 p-형 전하 도핑을 유도한 그래핀에서는 기계적 변형과는 분명히 구별되는 상관관계(${\Delta}{\omega}2D/{\Delta}{\omega}G$ = 1.0 ${\pm}$ 0.3)가 관찰되었다. 본 연구는 라만 분광법을 통해 그래핀의 기계적 변형과 전하 도핑 정도를 정량적으로 분리해서 분석할 수 있는 방법을 제시해 준다.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.11 s.254
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• pp.1417-1424
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• 2006

In order to evaluate variation of fatigue life of mechanical components including engineering plastics, it is important to estimate probabilistic strain-life curves to accurately define the variation of fatigue characteristics. This paper intends to provide new assessment of P-$\varepsilon$-N (probabilistic strain-life curves) for considering the variation of fatigue characteristics in polyacetal. The fatigue strain controlled tests were conducted under constant 50% humidity and room temperature condition by a universal testing machine at strain ratio, R=0. A practical procedure is introduced to evaluate probabilistic strain-life curves. Three probabilistic distributions were used for generating P-$\varepsilon$-N curves such as normal, 2-parameter and 3-parameter Weibull. In this study, 3-parameter Weibull distribution was found to be most appropriate among assumed distributions when the probability distributions of the fatigue characteristic were examined using chi-square and Kolmogorov-Smirnov test. The more appropriate P-$\varepsilon$-N curves for these materials are generated by the proposed method considering 3-parameter Weibull distribution.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1997.04a
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• pp.49-61
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• 1997

본 연구에서는 한국에서 구삼각강(1910-1915)과 정밀1차측지망(1975-1994)을 사용하여 지각변동량을 계산하고, 지체구조의 일반적인 변형패턴을 기하학적으로 조사하였다. 본 연구에서는 변형량을 계산하기 위하여 2차원의 무한소 변형모델을 설정하였으며, 수평변형량은 구좌표와 정밀1차측지망의 정밀동시강조정을 자유강조정법에 의하여 최초로 실시하여 일괄성있는 신좌표를 사용하여 계산된 측지선의 변화량으로 추정하였다. 변형설계결과로부터 1910년부터 1994년까지 누적된 변형량은 평균(1.07$\pm$0.5)$\times$10-5이고, 이로부터 년변형속도는 (0.13$\pm$0.063)$\mu$/yr 임을 알 수 있었으며, 변형의 경향을 보면 변형량이 10$\mu$ 보다 큰 값이 한반도의 동해안 지역에 분포하고 있으며, 서부쪽에는 10$\mu$이하의 값이 분포하고 있는 것으로 나타나 한반도의 동해안에서 지진의 발생빈도가 높은 것을 고려한다면 본 연구로부터 계산된 결과는 장래의 연구를 위해 중요한 데이터가 될 것이다. 본 연구에서 얻은 주변형축의 방향은 전국적으로 $77.6^{\circ}$$\pm$$13.5^{\circ}$방향임을 보여주고 있어 한반도의 지각은 ENE~WSW방향으로 압축상태에 있음을 알 수 있었으며, 이 결과는 지질학자나 지진학자들의 연구로부터 얻은 결과와 P-축의 방향이 일치하고 있고, 최대전단변형 이론과 일치하고 있는 것으로 나타났다.

• Journal of Korean Orthopaedic Sports Medicine
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• v.11 no.2
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• pp.82-86
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• 2012

Purpose: The purpose of this study was retrospectively to evaluate the results of the modified Kidner procedure for symptomatic accessory navicular in athletes. Materials and Methods: Between July 1999 and December 2004, 26 feet in 22 patients with symptomatic accessory navicular who had underwent modified Kidner procedure were available for clinical follow-up, and 12 cases in 9 patients were available for clinical and radiological follow-up with a minimum follow-up of 5 years were included in this study. All those patients had symptomatic accessory navicular bone who underwent modified Kidner procedure. American Orthopaedic Foot and Ankle Society (AOFAS) midfoot score, Visual Analogue Scale (VAS), and satisfaction rate were investigated. Talo-first metatarsal (T-MT1) angle, talo-calcaneal (TC) angle, and calcaneal pitch (CP) angle were measured in standing lateral radiograms. Results: AOFAS score was improved from $40.8{\pm}7.5$ (32~57) preoperatively to $88.7{\pm}8.0$ (72~100) postoperatively, and the difference was significant (p<0.01). VAS was improved from $7.0{\pm}0.9$ (5~9) preoperatively to $1.8{\pm}0.8$ (1~4) postoperatively, and the difference was significant (p<0.01). At the lastest follow up, 11 feet were very satisfied, 11 feet satisfied, and 4 feet unsatisfied (a satisfaction rate 85.0%). No significant difference was observed for T-MT1 angle (p=0.67), TC angle (p=0.93), and CP angle (p=0.49). Conclusion: Modified Kidner procedure for the symptomatic accessory navicular showed satisfactory results and is appeared to be one of the useful treatments.

• Communications of Mathematical Education
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• v.25 no.2
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• pp.381-402
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• 2011

In this paper we study formulae of the triangle's area. We solve problems related with making new formulae of the triangle's area. These formulae is consisted of some elements of triangle, for example side, angle, median, perimeter, radius of circumcircle. We transform formulae $S=\frac{1}{2}acsinB$, $S=\frac{abc}{4R}$, $S=\sqrt{p(p-a)(p-b)(p-c)}$, and make new formulae of the triangle's area. Some formulas are received in the process of Research and Education program in the science high school. We expect that our results will be used in the Research and Education program in the science high school.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.05a
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• pp.796-802
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• 1995

본 연구의 목적은 원자력 배관용 스테인레스강의 J-R곡선을 예측하기 위한 2가지 방법 올 제시하는 것이다. 첫 번째 방법에서는 균열길이/시편폭 비를 변수로 한 탄소성 유한요소해석을 수행하여 파괴변형률에 근거한 P-$\delta$곡선을 얻고, 이 결과로부터 일반궤적법을 응용하여 J-R곡선을 구하였다. 두 번째 방법에서는 $\sigma$-$\varepsilon$곡선과 J-R곡선의 상관관계를 통계처리하여 응력-변형률시험결과로부터 J-R곡선을 예측할 수 있는 실험식을 제시하였다. 본 연구에서 제시한 방법들을 이용하여 구한 예측결과는 실험결과와 대체로 잘 일치하였다.

• Korean Journal of Food Science and Technology
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• v.31 no.5
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• pp.1262-1267
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• 1999

Optimum reaction conditions for gel formation of rapeseed, Brassica napus, protein catalyzed by microbial TGase(transglutaminase) were evaluated by measuring breaking strength and deformation of gel. The polymerization of the protein gel was ascertained by SDS-PAGE and content of GL crosslinking$[{\varepsilon}-({\gamma}-glutamyl)lysine]$. In the reaction between rapeseed protein and TGase at $45^{\circ}C$ for 60 min, the breaking strength and deformation of the gel was the maximum at the ratio of 1 : 40 of enzyme to substrate. 10%(w/v) of rapeseed protein concentrate was optimum for gel production. The maximum breaking strength and deformation was shown at $45^{\circ}C$ The breaking strength increased linearly up to 90 min of the reaction time and remained unchanged. The breaking strength and deformation by TGase treatment was pH dependent and pH 7 was optimum for 10% rapeseed protein solution. SDS-PAGE analysis indicated that new band of highmolecular polymers were formed by the enzyme reaction, with disappearing the original bands of rapeseed protein. According to HPLC analysis. the content of GL crosslinking was increased from 0 to $7.14\;{\mu}mol/g$ gel for 90 min of the reaction time.

• Journal of Internet of Things and Convergence
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• v.6 no.2
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• pp.25-29
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• 2020

The elliptic curve crypto-algorithm is widely used in authentication for IoT environment, since it has small key size and low communication overhead compare to the RSA public key algorithm. If the scalar multiplication, a core operation of the elliptic curve crypto-algorithm, is not implemented securely, attackers can find the secret key to use simple power analysis or differential power analysis. In this paper, an elliptic curve scalar multiplication algorithm using a randomized scalar and an elliptic curve point blinding is suggested. It is resistant to power analysis but does not significantly reduce efficiency. Given a random r and an elliptic curve random point R, the elliptic scalar multiplication kP = u(P+R)-vR is calculated by using the regular variant Shamir's double ladder algorithm, where l+20-bit u≡rn+k(modn) and v≡rn-k(modn) using 2^lP=∓cP for the case of the order n=2^l±c.