• KSBB Journal
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• v.23 no.6
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• pp.513-518
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• 2008

This paper described the extraction/purification of $\beta$-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified $\beta$-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of $\beta$-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of $\beta$-carotene corresponding to 61.2% of recovery. The formation and separation of $\beta$-carotene crystal improved the purity. 633 mg of $\beta$-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of $\beta$-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. $1.7{\mu}M$ of $\beta$-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of $\beta$-carotene. This indicates that highly purified $\beta$-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.

• Journal of Korea Water Resources Association
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• v.45 no.6
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• pp.531-544
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• 2012

This study suggested the parameter estimation method for given rainfall events to be properly expressed by the beta distribution. For this purpose, this study compared the characteristics of probability density function with the parameter proposed considering the cases with and without addition to the rainfall peak, and the cases of using the real hyetograph and the rearranged hyetograph about the rainfall peak. As an example, this study analyzed the independent rainfall events at Seoul in 2010 and the annual maximum independent rainfall events from 1961 to 2010. The results derived are as follows. First, this study confirmed the necessity of additional consideration on rainfall peak to mimic the real hyetograph of rainfall events by the beta distribution. Second, this study confirmed the case of using rearranged hyetograph about the rainfall peak derived a better beta distribution to well mimic the characteristics of real rainfall than the case using the real hyetograph.

• The Korean Journal of Financial Management
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• v.24 no.4
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• pp.75-106
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• 2007

In this study, it is analyzed whether oil price plays a major role in the pricing return on Koran stock market and examined why the covariance risk between oil and return on stock is different in each industry. Firstly, this study explores whether the expected rate of return on stock is pricing due to global oil price factors as a function of risk premium by using a two-factor APT. Also, it is examined whether spill-over effects of oil price volatility affect the beta risk to oil price. Considering the asymmetry of oil price volatility, we use the GJR model. As a result, it shows that oil price is an independent pricing factor and oil price volatility transmits to stock return in only electricity and electrical equipment. Secondly, the two step-analyzing process is introduced to find why the covariance between oil price factor and stock return is different in each industry. The first step is to study whether beta risk exists in each industry by using two proxy variables like size and liquidity as control variables. The second step is to grasp the systematic relationship between the difference of liquidity and size and beta to oil price factor by using the panel-data model which can be analyzed efficiently using the cross-sectional data formed with time series. Through the analysis, we can argue that oil price factor is an independent pricing factor in only electricity and electrical equipment having the greatest market capitalization, and know that beta risk to oil price factor is a proxy of size in the other industries. According to the result of panel-data model, it is argued that the beta to oil price factor augments when market capitalization increases and this fact supports the first assertion. In conclusion, the expected rate of return of electricity and electrical equipment works as a function of risk premium to market portfolio and oil price, and the reason to make beta risk power differentiated in each industry attributes to the size.

• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.399-404
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• 2009

This paper described the formulation of functional cosmetics and evaluation of anti-wrinkle efficacy in clinical test. Cosmetics were formulated with highly purified $\beta$-carotene obtained from the culture broth of recombinant E.coli cells. Edible oil for solubilizing $\beta$-carotene, vitamine E for long-term storage, detergent/stabilizer (2.0%) for the complete formation of oil/water emulsion, dimethicone (0.35%) for good skin care, and sorbitol for skin moisturizer were also added as ingredients. Physical or chemical degradation of formulated products stored at $0^{\circ}C$, $25^{\circ}C$, or $40^{\circ}C$ was not observed for 60-day testing period. In clinical test, 68% of applicants observed wrinkle decrease after 8-week treatment. This result indicates that newly formulated cosmetics have strong potential for improving wrinkle skin care.

• Proceedings of the Materials Research Society of Korea Conference
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• 2007.05a
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• pp.39.2-39.2
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• 2007

• The Korean Journal of Financial Management
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• v.9 no.2
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• pp.57-75
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• 1992

본 논문은 10개의 기업규모별 자산을 대상으로 최근에 발전된 계량기법인 GMM(generalized method of moments)을 이용하여 베타(beta)를 추정하였다. 분석대상기간으로 $1982.1{\sim}1991.4$사이의 월별자료를 사용한다. 실증분석 결과에 의하면, 기업규모별 구분에 따른 자산의 경우에 규모가 큰 기업보다 규모가 작은 기업의 베타가 상대적으로 작은 것으로 나타났다. GMM의 추정을 위한 수단변수로 회사채수익률과 정기예금금리의 금리차, 분석대상이 되는 자산 수익률과 시장포트폴리오의 자기시차, 그리고 상수가 사용되었다. OLS를 사용한 CAPM추정 결과에 비해 GMM을 사용한 추정 결과가 우월할 수 있음을 보여주고 있는데, 이것은 GMM에 사용된 수단변수들이 수단변수를 포함시킴으로써 관련자산들의 자기시차가 아닌 CAPM추정에 필요한 유용한 대용변수(代用變數)(proxy)를 제공하였고, 나아가 GMM이 잔차항(殘差項)의 자기상관(自己相關) 뿐만 아니라 조건부(條件附) 이분산(異分散)(conditional heteroskedasticity)을 잘 설명하고 있기 때문인 것으로 판단된다. t값 및 P-value에 의하면 GMM을 사용한 단순 CAPM 추정이 우리 나라의 현실경제와 잘 부합될 수 있음을 암시한다.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.11b
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• pp.583-589
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• 1996

원자력발전소에서 발생되는 방사성폐기물들은 폐기물형태 및 방사능 농도가 다양하며 영구처분장으로 이송전까지는 발전소내의 임시 저장고에 안전하게 보관, 관리하고 있다. 생성된 폐기물드럼내에는 감마방출핵종을 비롯하여 알파 및 베타방출 핵종들이 균질 또는 비균질하게 존재하고 있으며 방사능의 세기나 폐기물의 특성에 따라 안정화시키거나 압축처리하여 드럼에 담겨져 있기 때문에 일반적인 파괴분석에 의한 화학분석법으로는 작업자의 피폭, 시료의 대표성 선정 및 장시간의 화학처리 시간소요 등으로 핵종분석이 곤란하다. 따라서 본 논문은 일반적으로 감마핵종분석시 흔히 사용하고 있는 고순도게르마늄(HPGe) 검출기를 이용하여 드럼의 감마핵종농도를 분석하는 방법과 장치의 개발에 대해 언급하였으며 알파나 베타핵종과 같이 직접 분석이 곤란한 핵종들은 각 폐기물드럼내에 존재하는 Co-60이나 Cs-137과의 상관관계를 미리 예측한 척도인자 (scaling factor)를 이용하여 간접적으로 구하는 방법을 사용하고 있으나 본 논문에서는 드럼으로부터 감마핵종만을 분석하는 방법에 대해서만 언급하였다. 또한 핵종분석시스템의 최적 운전조건을 도출하기 위해 드럼회전테이블의 속도결정 및 모의드럼을 이용한 방사능측정 등을 통해 핵종 농도 분석시의 오차를 30% 이내로 유지할 수 있었다.

• Journal of the Korean Chemical Society
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• v.40 no.5
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• pp.357-364
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• 1996

Stereochemistries of acetoxy 1,3-oxathiolane 1 were determined by two methods. First, the structures of $\alpha$ isomer 7 and $\beta$ isomer 9 were confirmed by the difference of their conversion rates to dihydrooxathiin 2 under acid catalysis. When the acetoxy leaving group is located in trans relationship to sulfur, a isomer in which carboxanilide is less hindered sterically against the 1,3-oxathiolane ring is $\beta$ isomer 7, and the other isomer of which the reaction rate is slower than 7 is $\beta$ isomer 9. Second, in the deuterium reactions of diastereomeric sulfoxides, the isomers of which methine hydrogen is substituted to deuterium were cis isomers 15 and 17, and another isomers of which methyl hydrogen is substituted to deuterium were trans isomers 16 and 18. Substitution of either methine or methyl hydrogen to deuterium resulted from stereospecific ring opening followed by recyclization by [2,3] sigmatropic rearrangement.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.12
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• pp.5671-5676
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• 2011

Volatility Index is the index that represents future volatility of underlying asset implied in option price and expected value of market that measures the possibility of stock price's change expected by investors. The Korea Exchange announces a volatility Index, VKOSPI, since April, 13, 2009. This paper used daily data from January, 2002 through December, 2008 and tested power of Volatility index for future returns of portfolios sorted by size, book-to-market equity and beta. As a result, VKOSPI has the predictive power to future returns and then VKOSPI may be determinants of returns. Also if beta is included when sorting portfolio, the predictive power of VKOSPI is stronger for future portfolio returns.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.165-169
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• 2014

Salmonella spp. and Brucella spp. are associated with considerable diseases of both humans and animals. In addition, these microorganisms cause the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a commercial disinfectant, composed to povidone-iodine was evaluated against S. typhimurium and B. ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW condition, the bactericidal activity of the disinfectant against S. typhimurium and B. ovis was 400 and 150 fold dilutions, respectively. On OM condition, the bactericidal activity of the disinfectant was 5 and 20 fold dilutions against S. typhimurium and B. ovis, respectively. As the disinfectant composed to povidine-iodine possesses bactericidal efficacy against animal pathogenic bacteria such as S. typhimurium and B. ovis, the disinfectant solution can be used to control the spread of bacterial diseases.