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Anti-wrinkle Activity of $\beta$-carotene Extracted & Purified from Recombinant Escherichia coli  

Jo, Ji-Song (Department of Chemical & Biological Engineering and ERI, GyeongSang National University)
Ku, Bo-Mi (Department of Anatomy & Neurobiology, GyeongSang National University)
Kang, Sang-Soo (Department of Anatomy & Neurobiology, GyeongSang National University)
Lee, Jae-Ran (Department of Chemical & Biological Engineering and ERI, GyeongSang National University)
Kim, You-Geun (KB Cosmetics)
Lee, He (Department of Chemical & Biological Engineering and ERI, GyeongSang National University)
Kim, Sung-Bae (Department of Chemical & Biological Engineering and ERI, GyeongSang National University)
Kim, Seon-Won (Division of Applied Life Science, GyeongSang National University)
Kim, Chang-Joon (Department of Chemical & Biological Engineering and ERI, GyeongSang National University)
Chung, In-Young (Department of Electronics and Communications, Kwangwoon University)
Publication Information
KSBB Journal / v.23, no.6, 2008 , pp. 513-518 More about this Journal
Abstract
This paper described the extraction/purification of $\beta$-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified $\beta$-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of $\beta$-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of $\beta$-carotene corresponding to 61.2% of recovery. The formation and separation of $\beta$-carotene crystal improved the purity. 633 mg of $\beta$-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of $\beta$-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. $1.7{\mu}M$ of $\beta$-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of $\beta$-carotene. This indicates that highly purified $\beta$-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
Keywords
Recombinant Escherichia coil; $\beta$-carotene; Extraction; Anti-wrinkle activity; Functional cosmetics;
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Times Cited By KSCI : 1  (Citation Analysis)
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