• Journal of Mushroom
• /
• v.19 no.3
• /
• pp.140-149
• /
• 2021

Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.

• Journal of Mushroom
• /
• v.14 no.2
• /
• pp.44-50
• /
• 2016

In the present study, we analyzed the amino acid and free sugar contentof Flammulina velutipes. We observed that the free sugar content in Flammulina velutipes increased under higher storage temperatures. However, the the packaging material had no effect on the sugar content. Higher storage temperature increased the level Among the packaging film materials tested for the storage of Flammulina velutipes, polyphenol oxidase. ${\beta}$-glucan level of Flammulina velutipes by variation in the packaging film material or storage temperature. We suggest that the ${\beta}$-glucan content is not an acceptable to mushroom storage standards.

• Journal of Mushroom
• /
• v.4 no.1
• /
• pp.39-42
• /
• 2006

Cauliflower mushroom(Sparassis crispa) contains much amount of ${\beta}$-glucan, which make lots of farmer want to cultivate the mushroom. Since a practical cultivation method is not provided yet, the mushroom is considered as a difficult crop to deal with. In this study, we tried to develope a simple method to cultivate the mushroom by use of coniferous sawdust-based medium with wheat flour and molasses. There was no significant differences between the sawdust spawn and the liquid spawn for the mycelial growth of the mushroom. The cold shock in $4^{\circ}C$ for a day was thought to be the best way to seduce primordium formation. The sawdust medium of Pseudotsuga menziesii mixed with wheat flour, corn chip, cottonseed meal and 10% molasses was showed the best yield with 41% followed by that of Larix leptolepis mixed with the same additives with 37% of yield. There was good relations between the fruit-body production and the weight loss of the sawdust substrate.

• Journal of Mushroom
• /
• v.8 no.1
• /
• pp.1-5
• /
• 2010

The multiple functions of mushrooms are production of antibiotics, fodder for animal stock, organic fertilizers, food nutrients, favorite food, physiologically active materials, and decomposing organic matters, and cleaning environmental pollutants. Of these first three functions were evaluated. The value of the muti-functions of mushrooms were estimated to be 112 trillion 922 one hundred million Won, and if non-evaluated ones added the estimation would be doubled. This value is similar to multi-functions value of rice, 100 trillion Won. Especially, the industrial value of antibiotics from B-glucans is estimated to be 112 trillion Won, while animal stock fodder value is 618 one hundred million Won, organic fertilizer 56 one hundred million Won and decomposer of ecology 360 one hundred million Won.

• The Korean Journal of Food And Nutrition
• /
• v.23 no.2
• /
• pp.141-146
• /
• 2010

Cisplatin(cis-diamminedichloroplatium) is one of the most effective anti-cancer drugs being clinically used in the treatment of solid tumors. Despite its therapeutic benefits, its use in clinical practice is often limited because of dose related toxicity. It is known that yeast cell wall beta-glucans possess immuno-modulating properties, which allows for their application in antitumor therapy. IS2 is a kind of beta-glucan derived from the cell wall of mutated Saccharomyces cerevisiae, which exhibits anti-cancer activity in vitro and in vivo. The present study explored the possibility of combination therapy of IS2 with cisplatin. In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous administration of IS-2 in combination with cisplatin effectively inhibited tumor metastasis compared with cisplatin alone or IS-2 treatment in vivo. IS-2 effectively enhanced Th1 type cytokines including IFN-$\gamma$, IL-2, IL-12 and GM-CSF. Simultaneously, this combined treatment inhibited production of Th2 type cytokines compared with control. These results suggested that IS-2 can be applied in combination therapy with anti-cancer drugs to minimize their side effects.

• Journal of Korea Water Resources Association
• /
• v.53 no.3
• /
• pp.167-179
• /
• 2020

Recently, new levee material has been developed to enhance natural soil strength and vegetation growth using biopolymer. In the study, critical tractive force of vegetated mats mixed with biopolymer mixed soil has been evaluated to apply the mixed soil to levee construction material. The mixed soil has been produced by mixing beta-glucan, clay, and sand. Full scale test bodies have been constructed with 3 cm thick of the mixed soil. Total 4 test bodies have been constructed and experimented. Critical tractive forces have been evaluated by observation and measurement of failure conditions and soil loss. Although performance of the vegetated revetments are affected by vegetation coverage conditions, the critical tractive forces are shown about 40 N/㎡ and the critical velocities are shown about 4 m/sec by full scale experiment. Erosion resistance is also enhanced by combination of root and net with mat materials.

• Journal of Radiation Industry
• /
• v.4 no.3
• /
• pp.233-237
• /
• 2010

This study evaluated the change in whitening activity of ${\beta}-glucan$ by gamma-irradiation. Tyrosinase inhibition was significantly increased in the samples with 30, 50, 100 kGy irradiated ${\beta}-glucan$. Melanin synthesis of irradiated ${\beta}-glucan$ was measured from B16BL6 melanoma cell line treated with ${\alpha}-melanin$ stimulating hormone. Melanin synthesis was increased in the ${\alpha}-melanin$ stimulating hormone added group. However, it was decreased in the groups of 30, 50 and 100 kGy gamma-irradiated ${\beta}-glucan$ treated with ${\alpha}-melanin$ stimulating hormone. These results indicate that gamma irradiated ${\beta}-glucan$ may elevate the whitening activity. Therefore, gamma-irradiated ${\beta}-glucan$ could be used for nutraceutical foods in cosmetic industry.

• Journal of Mushroom
• /
• v.17 no.3
• /
• pp.107-112
• /
• 2019

This study was conducted to develop a healthy functional food material using mycelial extract of Phellinus sp. Jangsoo and GMPL48007 were selected as suitable strains because of good mycelial growth and high functionality. The highest total polyphenols and reducing power were observed in the Jangsoo strain and the radical scavenging was shown in GMPL48007. Among grains media, whole-grain wheat had the highest extraction yield from mycelia of selected strains; especially, the GMPL48007 strain showed the highest content of beta-glucan.

• Journal of The Korean Society of Integrative Medicine
• /
• v.12 no.2
• /
• pp.141-153
• /
• 2024

Purpose : This study aimed to determine the effects of a 12-week program of beta-glucan intake and muscle strengthening exercises on the body composition, physical fitness, blood lipids, insulin and insulin resistance of adult women. Methods : For this study, 45 adult women were selected and divided into two groups : the experimental group (n= 25), which received a combination of beta-glucan and strength exercise, and the control group (n= 20), which received only strength exercise. The program was conducted for 50 minutes three times a week for 12 weeks. Results : Several significant differences were found in both groups pre-to post program. First, in both groups, there were significant differences in hip circumferences (p<.05). Second, the results showed significant improvements in left grip strength and sit-ups achieved (p<.05), right grip strength (p<.001). Third although the results show no significant post-program differences in either group, there was a significant pre-to post-program difference in free fatty acids in both groups (p<.05). Fourth, the results show no significant differences in insulin or insulin resistance in either group, nor do they show any significant differences between the groups. Conclusion : The above results indicate that both groups exhibited decreased hip circumference and increased muscle strength (left grip strength) and muscle endurance. Furthermore, they indicate that when the beta-glucan intake and exercises were combined, the effects were more pronounced. Finally, because both groups exhibited significant increases in fat-free mass, muscle mass, skeletal muscle mass, muscle strength (right grip strength), and coordination as well as decreases in free fatty acids, it can be concluded that both the combined program of beta-glucan intake and exercise and a program of exercise alone can positively affect these outcomes.

• Journal of Life Science
• /
• v.24 no.11
• /
• pp.1209-1216
• /
• 2014

The aim of this study was to investigate the effect of water-soluble sulfated ${\beta}$-glucan (SBG) obtained from Ganoderma lucidum mycelia on the antihyperlipidemic and serum lipid levels in high-fat diet-induced obese rats. Five-week-old male Sprague-Dawley rats were fed a high-fat diet for two weeks to induce obesity. They were ten divided into five groups-normal control diet group (NC), high-fat control diet group (HC), high-fat diet and 200 mg/kg of SBG group (HC-HSBG), high-fat diet and 20 mg/kg of SBG group (HC-LSBG), and high-fat diet and 20 mg/kg of lovastatin group (HC-Lov)-and fed one of five diets for two more weeks. Although food intake and final body weight after four weeks of SBG consumption were similar in the five experimental groups, food efficiency ratio was higher in the high-fat diet groups(2, 3, 4, and 5) than in the NC group. In evaluating the hematological parameters of the rats, the neutrophil and monocyte ratios were higher in the HC-HSBG, HC-LSBG, and HC-Lov groups than in the HC group. Serum lipid profiles were analyzed after a 12 hr fast at the end of the study. Total cholesterol, triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels were significantly lower in the HC-HSBG and HC-LSBG groups than in the HC group. These results suggest that chemically engineered sulfated mushroom ${\beta}$-glucan (SBG) might contribute to lower cholesterol and lipid levels in blood.