• Journal of Life Science
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• v.23 no.4
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• pp.586-594
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• 2013

Post-translational O-GlcNAc modification (O-GlcNAcylation) of serine or threonine is a new protein modulation mechanism. In contrast to the classical glycosylation, O-GlcNAcylation occurs in a one-step transfer of O-GlcNAc on both nuclear and cytoplasmic proteins. In contrast to the general consensus that O-GlcNAc is a final modification, a recent paper (J Proteome Res. 2011 10:2725-2733) showed the presence of O-GlcNAc-P on a synaptic assembly protein AP180. This finding raises a fundamental question about its prevalence. To address this question, we used proteomics to identify those proteins that were phospho-signal enriched by GlcNAc kinase (NAGK). Comparison of pDsRed2-$NAGK_{WT}$-transfected HEK293T cell extract with pDsRed2-$NAGK_{D107A}$-transfected control culture revealed 15 phospho-signal increased spots. Excluding those spots that had no detectable amount of protein expression yielded 7 spots, which were selected for ID determination. Among these, two duplicate spots (two $HSP90{\beta}$ and two ENO1 spots) were shown to be O-GlcNAcylated, two (dUTP nucleotidohydrolase mitochondrial isoform 2, glutathione S-transferase P) were not known to be involved in O-GlcNAcylation, and one (heat shock protein gp96 precursor or grp94) was a glycoprotein. The increase in the phospho-levels of O-GlcNAc by NAGK strongly indicates that these proteins are phosphorylated on O-GlcNAc. Our present data support the idea that O-GlcNAc is not a terminal modification.

• Journal of Gifted/Talented Education
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• v.19 no.3
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• pp.585-602
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• 2009

The purpose of this study was to compare the 5 OEs of Korean gifted elementary students and non-gifted and to find out the gender difference in gifted and non-gifted to clarify the best predictor of giftedness in gender group. The total subjects of this study were 372 Korean third, fourth, and fifth grade elementary school children. One hundred sixty six children of them belonged to a gifted group, and 206 were non-gifted group. Two hundreds one out of 372 subjects were boys and 171 were girls. The Overexcitability Questionnaire II(OEQII) used in this study was developed by Falk, Lind, Miller, Piechowski, & Silverman in 1999. The OEQ II is consisted of 50 Likert-type items, classified into five subscale: psychomotor, sensual, imaginational, intellectual, and emotional. And it was translated into Korean. It was administered in group sessions during the winter of 2008. Data were analyzed with SPSSWin 12.0. MANOVA was conducted to determine statistical differences by giftedness and by gender. The results revealed that the level of 5 OEs of gifted elementary children was higher than non-gifted(normal) children. The intellectual OE showed the most powerful explanation among the 5 forms of OEs. This study also found the gender difference within the gifted group as well as with the whole subjects. The mean scores of sensual, imaginational and emotional OEs were higher in girls than in boys. Based on the gender difference within the gifted group and the whole group, it is assumed that imaginational OE may be the best predictor of gifted girls among school-age children.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.26 no.3
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• pp.83-100
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• 2015

This paper aims to survey the supply status and demand of the scientific & technological information among the 1,059 small enterprises demanding the support in Busan, Ulsan and Gyeongsangnam-do which have been supported by the Branch of KISTI and to propose the approaches to improve the scientific & technological information system. As the results of this research, Busan, Ulsan and Gyeongsangnam-do have very poor business and R&D environment to the extent that 96.3% of businesses was small business (medium business only for 3.7%) and 31% of total small & medium-cized businesses has less than 10 R&D workers. Small & medium-sized businesses which have demanded the information to KISTI account for only 0.2% in average for 3 years. The information demand types are market trend (26.8%), industrial property right (22.1%), business proposal (19.9%), item exploration (16.8%) and quality certification (8.4%). The supplied informations include scientific and technological data survey, market survey, translation and information application seminar. The business achievements from the information supply include 379 cases in technological development improvement and launch of products, 88 cases in technology certifications and 414 cases in patents and utility design application and registration. By the analysis results, the small and medium-sized businesses have difficulties in using R&D information by themselves. Thus, it's proposed to establish the science and technology information center in southeastern region and develop the information advisors specialized in local specialty industry categories. It's also proposed to expand the manpower and budget of KISTI concerning the information support for small & medium-sized businesses.

• Phonetics and Speech Sciences
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• v.13 no.4
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• pp.35-46
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• 2021

In this study, we examined the interactive aspects between pitch reduction phenomena considered a universal language phenomenon and native language interference in the production of L2 intonation performed by Chinese learners of Korean. To investigate their interaction, we conducted an acoustic analysis using acoustic measures such as pitch span, pitch level, pitch dynamic quotient, skewness, and kurtosis. In addition, the correlation between text comprehension and pitch was examined. The analyzed material consisted of four Korean discourses containing five and seven sentences of varying difficulty. Seven Korean native speakers and thirty Chinese learners who differed in their Korean proficiency participated in the production test. The results, for differences by language, showed that Chinese had a more expanded pitch span, and a higher pitch level than Korean. The analysis between groups showed that at the beginner and intermediate levels, pitch reduction was prominent, i.e., their Korean was characterized by a compressed pitch span, low pitch level, and less sentence internal pitch variation. Contrariwise, the pitch use of advanced speakers was most similar to Korean native speakers. There was no significant correlation between text difficulty and pitch use. Through this study, we observed that pitch reduction was more pronounced than native language interference in the phonetic layer.