• Horticultural Science & Technology
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• v.19 no.3
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• pp.352-357
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• 2001

This experiment was carried out in order to develop some chip budding techniques related to the production of high quality maiden apple trees. Optimum chip budding time for apple trees appeared to be from mid-August to early September. Untying wrapping tapes after 6 8 weeks from budding led to higher graft success and better shoot growth than removing the tapes earlier or later than the 6-8 weeks. Dipping pre-cut bud chips into ordinary water of $25^{\circ}C$ for 5 hours did not hamper the graft-success nor the early season growth of the graft compared with non-treated control. Dipping the pre-cut bud-chips into the $35^{\circ}C$ water for 3 hours or for 5 hours hampered the union formation and shoot growth of the 'Fuji' apple trees.

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.212-220
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• 2022

When it comes to single node leafy stem cuttings of rose (Rosa hybrida), environmental management such as air temperature, relative humidity, and light affect productivity. In order to investigate the effect of air temperature and relative humidity on the cutting success rate and rooted cuttings quality, a transparent airtight box was used to implement a closed system. We have also tried to find out the most effective photoperiod and the number of leaflets in closed system using artificial light (white LED, 104.0 µmol·m^-2·s^-1 photosynthetic photon flux density). The first experiment was conducted for a total of 6 weeks under 4 airtight period conditions. The number of roots and longest root length decreased as the airtight period increased. But there were no significant differences in the survival rate, shooting rate, and rooting rate according to airtight periods. In the second experiment the results indicated that survival and shooting rate were significantly affected by the photoperiod (0/24, 2/22, 4/20, 8/16, and 16/8 h), the number of leaflets (0, 2, and 4 leaflets) of the cuttings and their interaction. The survival rate was the highest in the 16-h day length and 4 leaflets. By considering survival rate and shooting rate with energy efficiency, the 8-h day length and 2 or 4 leaflets were judged to be the most effective.

• Reproductive and Developmental Biology
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• v.30 no.1
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• pp.1-5
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• 2006

Artificial activation of oocytes is a prerequisite for the successful cloning by nuclear transfer. This study investigated the effect of the different combination of activation agents such as electric pulse (E), thimerosal (Thi) + dithiothreitol (DTT), 6-dimethylaminopurine (6-DMAP) or cycloheximide (CH) on the developmental ability of porcine embryos derived from parthenogenetic activation (PA). PA embryos activated with chemicals showed significantly higher developmental rate to the blastocyst stage compared to the embryos activated with E alone ($21.5{\sim}28.1%$ vs. 18.0%, respectively). Of chemicals, Thi + DTT supported higher development to the blastoryst stage (28.1%). There was no significant difference in 1 pronucleus (PN) formation rate $(59.9{\sim}64.7%)$, but 2PN formation rate was significantly higher in PA embryos with additional activation using chemicals $(7.2{\sim}9.7%)$. In conclusion, this study shows that chemical activation after electric pulse can increase the development of porcine PA embryos.

• Korean Journal of Plant Resources
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• v.36 no.2
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• pp.181-190
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• 2023

Domestic hop (Humulus lupulus L.) production has been suspended since the early 1990s due to foreign imports, but interest in local production is rising due to the recent craft beer boom in Korea. This study was conducted focusing on the development of growth characteristics and propagation technology for 6 introduced hop cultivars as a basic study for domestic hop production and breeding program. In the hop growth survey conducted in 2021 and 2022, the 5-year-old plants after planting generally showed a tendency to increase the height of strobile setting, strobile size, number and weight of strobile per hill compared to the 4-year-old plants. As a result of the experiment with hop vine cuttings, the average rooting rate of all cultivars was as high as 88% even in only water treatment that were not added with Atonik (Atonik, Arysta, Japan), a rooting agent. There were differences between cultivars in rooting length and rooting rate according to the Atonik treatment method. When checking the survival rate of the rooted cuttings seedlings after transplanting into the soil, it was confirmed that the survival rate of the cuttings in the tissue culture room was significantly lower than that of the cuttings in the greenhouse. However, in transplanting step, cutting plants from culture room condition was strongly inhibited plant growth because of changing environment conditions. As a results of tissue culture, the thidiazuron (TDZ) 1 ㎎/L treatment in the media generated 6 to 9 shoots/explant, while the 6-benzylaminopurine (BAP) 1 ㎎/L treatment generated only 1 to 2 shoots/explant. Therefore, it is more effective to culture by adding TDZ rather than BAP. These results indicated that the development of technology to manage stably after transplanting of cutting or micropropagating plants into potting soil is important for mass propagation of hops.