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Development of Porcine Parthenogenetic Oocytes Activated with Different Combination of Chemicals  

Seo Jin-Sung (National Livestock Research Institute, RDA, Suncheon National University)
Hwang In-Sun (National Livestock Research Institute, RDA)
Kim Se-Woong (National Livestock Research Institute, RDA)
Park Hyo-Suk (National Livestock Research Institute, RDA)
Kim Dong-Hoon (National Livestock Research Institute, RDA)
Yang Byoung-Chul (National Livestock Research Institute, RDA)
Kong Il-Keun (Suncheon National University)
Yang Boh-Suk (National Livestock Research Institute, RDA)
Im Gi-Sun (National Livestock Research Institute, RDA)
Publication Information
Reproductive and Developmental Biology / v.30, no.1, 2006 , pp. 1-5 More about this Journal
Abstract
Artificial activation of oocytes is a prerequisite for the successful cloning by nuclear transfer. This study investigated the effect of the different combination of activation agents such as electric pulse (E), thimerosal (Thi) + dithiothreitol (DTT), 6-dimethylaminopurine (6-DMAP) or cycloheximide (CH) on the developmental ability of porcine embryos derived from parthenogenetic activation (PA). PA embryos activated with chemicals showed significantly higher developmental rate to the blastocyst stage compared to the embryos activated with E alone ($21.5{\sim}28.1%$ vs. 18.0%, respectively). Of chemicals, Thi + DTT supported higher development to the blastoryst stage (28.1%). There was no significant difference in 1 pronucleus (PN) formation rate $(59.9{\sim}64.7%)$, but 2PN formation rate was significantly higher in PA embryos with additional activation using chemicals $(7.2{\sim}9.7%)$. In conclusion, this study shows that chemical activation after electric pulse can increase the development of porcine PA embryos.
Keywords
Porcine; Parthenogenetic activation; Thi+DTT; 6-DMAP; Cycloheximide;
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